Scaffolds embryonic cell differentiation. Medical search. Frequent questions

Scaffolds play an important role in tissue engineering as a substrate that can mimic the native extracellular matrix and the properties of scaffolds have been shown to affect the cell behavior such as the cell attachment, proliferation and differentiation. (wjgnet.com)
Interaction with extracellular ligands induces receptor clustering at the nanoscale, assembly of the signaling complexes in the cytosol and activation of downstream signaling pathways, regulating cell response. (ibecbarcelona.eu)
On the other hand, even if 3D aggregate culture methods are able to recapitulate in vitro the cell-extracellular matrix interactions properly observed in vivo, and the synthetic/natural matrix and scaffolds have biochemical and mechanical properties, in order to mimic the native extracellular matrix, both of these systems possesses some limitations and some methodological improvements are needed. (razavihospital.ir)
Czyz J, Wobus A. Embryonic stem cell differentiation: the role of extracellular factors. (razavihospital.ir)
Chemical factors such as soluble growth factors and insoluble components of the extracellular matrix are known to affect the differentiation of murine ESCs. (ox.ac.uk)
Approaches of bioengineering and regenerative medicine aim to create different types of materials, implants, or scaffold mimicking structure of extracellular matrix, functionalized with bioactive molecules or living cells. (hindawi.com)
The three key elements of regenerative medicine and tissue engineering are morphogenetic signals, stem cells, and scaffolds of extracellular matrix. (ucdavis.edu)
These elements include somatic cells (such as Leydig cells, myoid cells, and Sertoli cells), extracellular matrix (ECM) components (including laminin, collagen type IV and collagen type I), as well as growth factors and hormones [including bFGF, glial cell-derived nerve factor, glial cellderived nerve factor (GDNF), and testosterone] that are capable of forming a complex microenvironment where spermatogenesis occurs (6). (ijfs.ir)

In some cases, an increase in oligodendrocyte differentiation alongside neurons is further highlighted for functional improvements where the biomaterial was then tested for increased myelination both in vitro and in vivo. (karger.com)
At this time I also began working with mouse and human embryonic stem cells resulting in the publication of some of the first papers describing the osteogenic differentiation of mouse ES and human cells in vitro and in vivo . (nottingham.ac.uk)
2- Human cortical organoids develop the typical hallmarks of senescent cells when maintained in vitro for prolonged periods of time, and moderate upregulation of endogenous KL expression in cortical organoids inhibits neuronal senescence (Shaker MR et al, NPJ Aging and Mechanisms of Disease 2021). (edu.au)
A defined synthetic mixture of amino acids, salts, carbohydrates, vitamins and serum was shown to support cells in vitro[ 3 ], thus unifying a major variable in cell culturing experiments and providing a possibility for rapid development of this novel method. (intechopen.com)
Results: The processes by which re-aggregated adult single cell types or adult and embryonic explanted tissues are able to recapitulate embryogenesis in vitro, when cultured in adhesion or embedded in 3D gels, is not surprising and is clearly under the control of a reminiscent cellular memory which recapitulates early developmental stages. (razavihospital.ir)
Moscona A. The Development in Vitro of Chimeric Aggregates of Dissociated Embryonic Chick and Mouse Cells. (razavihospital.ir)
Kurosawa H. Methods for inducing embryoid body formation: in vitro differentiation system of embryonic stem cells. (razavihospital.ir)
Culture and differentiation of preadipocytes in two-dimensional and three-dimensional in vitro systems. (razavihospital.ir)
Our results suggest a fundamental role for mechanosensing in mammalian development and illustrate that the mechanical environment should be taken into consideration when engineering implantable scaffolds or when producing therapeutically relevant cell populations in vitro. (ox.ac.uk)
1 One strategy is to seed the appropriate cells on a biodegradable scaffold engineered with the desired mechanical properties, followed by stimulation of cell growth and differentiation in vitro, such that, on implantation in vivo, the engineered construct undergoes remodeling and maturation into functional tissue. (ashpublications.org)
The Biolaminin 521 cell culture substrates successfully recreate the biologically relevant hPSC milieu in vitro . (biolamina.com)
Discuss how we can use stem cells combined with biomaterials for in vitro and in vivo applications. (manchester.ac.uk)
Human testisderived cells (hTCs) are suitable candidates for the initiation of in-vitro spermatogenesis for these types of patients. (ijfs.ir)
As of 2004, several studies have shown that embryonic stem cells may restore the spermatogenesis and functional sperms in mouse and human, known as in vitro gametogenesis (IVG) (2-4). (ijfs.ir)

Therefore, researchers have begun exploring combined therapies with biomaterials that promote cell growth and differentiation while localizing cells in the injured area. (karger.com)
mEScell growth and differentiation on PLDLA-co-TMC membrane, demonstrated the cyto compatibility of this polymer, and provided evidence of minimal to no toxicity on the cells, allowing adhesion and cell proliferation, as well as differentiation into cardiomyocytes. (jscimedcentral.com)

1 Neural stem/progenitor cell proliferation and differentiation: role of sonic hedgehog and wingless/int-1 proteins. (vinastemcelllab.com)

2012 ) Transgenic enrichment of mouse embryonic stem cell-derived progenitor motor neurons. (neurotree.org)
Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. (razavihospital.ir)
In this study, the interaction of mouse embryonic stem cells (mESC) with poly L-co-D, L lactic acid co-trimethylene carbonate (PLDLA-co-TMC) was assessed. (jscimedcentral.com)

Preliminary biological studies were conducted to evaluate adhesion (6 h) of human mesenchymal stem cells (hMSC) to the patterns in terms of cell number and morphology. (ibecbarcelona.eu)
Mariano García Arranz has the following conflict of interest: MGA is inventor on 2 patents entitled "Identification and isolation of multipotent cells from non-osteochondral mesenchymal tissue" (10157355957US) and "Use of adipose tissue-derived stromal stem cells in treating fistula" (US11/167061). (wjgnet.com)
Mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) were derived from syngenic mouse bone marrow. (vinastemcelllab.com)
13 Proliferation of bone marrow-derived human mesenchymal stem cells: role of enamel matrix proteins. (vinastemcelllab.com)
16 Microencapsulation procedures for the immunoisolation of wharton's jelly mesenchymal stem cells: a review. (vinastemcelllab.com)
19 Mesenchymal stem cell-natural killer cell interactions. (vinastemcelllab.com)
23 Enhanced growth and metastasis of colon cancer: role of mesenchymal stem cells. (vinastemcelllab.com)
24 Proteomic characterization of mesenchymal stem cell-like populations derived from various tissue types. (vinastemcelllab.com)
26 Transplanted mesenchymal stem cells aid the injured brain through trophic support mechanisms.Index. (vinastemcelllab.com)
Different sources of living cells are described, as mesenchymal stem cells (MSCs), adipose-tissue derived stem cells, skin derived multipotent stem cells, or oral cavity MSCs, presenting compatible immunophenotype or morphology [ 2 , 3 ]. (hindawi.com)
Thus, the strategy of functionalization of nanofibers by nanoreservoirs of BMP-2 or BMP-7 showed a great efficiency for bone regeneration and increased the differentiation of MSCs (mesenchymal stem cells), accelerating the tissue regeneration in vivo [ 9 , 10 , 13 , 14 ]. (hindawi.com)

Induced pluripotent stem cells for neural tissue engineering. (ca.gov)
Induced pluripotent stem cells (iPSCs) hold great promise for cell therapies and tissue engineering. (ca.gov)
6 Induced pluripotent stem cells from human extra-embryonic amnion cells: role of dna methylation in mainting stemness. (vinastemcelllab.com)
Her project focuses on combining human pluripotent stem cells and biomaterials to create defined, 3D culture models to study the role of glycosaminoglycans in human development and diseases such as Multiple Osteochondroma. (merrylab.co.uk)
Promotes differentiation of cardiomyocytes from human pluripotent stem cells (PSCs) that have been induced to mesoderm by addition of Activin A and/or BMP4 (Ren et al. (stemcell.com)
To exploit the full potential of human pluripotent stem cells for regenerative medicine, developmental biology and drug discovery, defined culture conditions are needed. (nih.gov)
Our results indicate that synthetic substrates that recognize cell-surface glycans can facilitate the long-term culture of pluripotent stem cells. (nih.gov)
Biolaminin 521 (laminin-521 protein) is a key cell adhesion protein of the natural stem cell niche, expressed and secreted by human pluripotent stem cells (hPSCs) in the inner cell mass of the embryo. (biolamina.com)
Via integrin binding, Biolaminin 521 induces the PI3K/Akt signaling pathway, promoting high survival and robust long-term self-renewal of human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC). (biolamina.com)

This study demonstrates the effect of EBM-fabricated cp-Ti and Ti6Al4V porous scaffolds on hES-MPs behavior, in terms of cell attachment, growth and osteogenic differentiation. (diva-portal.org)
Displaying different chemical composition but similar surface properties, EBM-fabricated cp-Ti and Ti6Al4V scaffolds supported cell attachment and growth, and did not seem to alter the expression of genes involved in osteogenic differentiation and affect the alkaline phosphatase activity. (diva-portal.org)
Finally, we demonstrated that osteogenic differentiation of ESCs was enhanced on stiff substrates compared to soft substrates, illustrating that the mechanical environment can play a role in both early and terminal ESC differentiation. (ox.ac.uk)
The main interest of the use of bone marrow derived stem cells is their osteogenic potential for neoangiogenesis. (hindawi.com)
Clinical use of human embryonic stem cells (hESCs) in bone regeneration applications requires that their osteogenic differentiation be highly controllable as well as time- and cost-effective. (trinity.edu)
The main goals of the current work were thus (a) to assess whether overexpression of pluripotency regulator Forkhead Box D3 (FOXD3) can enhance the osteogenic commitment of hESCs seeded in three-dimensional (3D) scaffolds and (b) to evaluate if the degree of FOXD3 overexpression regulates the strength and specificity of hESC osteogenic commitment. (trinity.edu)
Tuning forkhead box D3 overexpression to promote specific osteogenic differentiation of human embryonic stem cells while reducing pluripotency in a three-dimensional culture system. (trinity.edu)

These biomembranes or scaffolds constitute a support for osteoblastic adhesion and proliferation, but also microenvironment for stem cells' chemotactism and differentiation [ 1 ]. (hindawi.com)
In this study, it was observed that the ion implantation stimulated the neural proliferation and the implantation of different ions on cell culture surfaces was essential to determine the effects of this technique on adhesion, proliferation, differentiation and apoptosis properties of cells in details. (omicsonline.org)
The adhesions between cultured cells and the material surface are called focal attachments or adhesion plaques. (omicsonline.org)

Aizawa, Y., N.D. Leipzig, T. Zahira, M.S. Shoichet (2008) The effect of immobilized platelet derived growth factor AA on neural stem/progenitor cell differentiation on cell-adhesive hydrogels. (karger.com)
Delivery of osteoinductive growth factors from degradable PEG hydrogels influences osteoblast differentiation and mineralization. (ucdenver.edu)
As a support to the project, Kasia will provide 3D models grown in peptide hydrogels for testing the methodology being developed by the team, as well as carry out phenotypic characterisation of the cells. (merrylab.co.uk)

however, stem cell injection alone has been unsuccessful in many areas of neural regeneration. (karger.com)
Overall, these results present the polymer as a potential scaffold to be exploited in cardiac tissue regeneration. (jscimedcentral.com)
Specifically, research is directed at fundamental characterization and obtaining a scientific understanding of structure and property relationships of novel materials and platforms for batteries, fuel cells, electrocatalysts, photoelectrocatalysts, degradable scaffolds, functionalization of scaffolds for tissue regeneration, drug and protein delivery, non-viral gene delivery, as well as embryonic stem cell differentiation. (pitt.edu)
These different nanofiber scaffolds with nanoreservoirs are efficient proregenerative biomimicking implants for bone regeneration. (hindawi.com)
Many studies have shown the utility of embryonic or adult stem cells for forming teeth and for regeneration of bone and soft tissues. (bvsalud.org)
Stem Cells, Dentistry, Regeneration. (bvsalud.org)
Stem cells are nonspecific cells with powerful self-regeneration properties and they are capable of organizing other cell types in the body. (bvsalud.org)
The present study results indicated that compared with the same number of 2D-cultured cells, microtissue could secrete more nerve regeneration related cytokines to promote SCs proliferation and axons growth. (biomedcentral.com)
Regeneration recapitulates embryonic development and morphogenesis. (ucdavis.edu)
Changing surface characteristics of scaffolds was a way to promote regeneration and ion implantation was one of the methods to modify surface properties which play a huge role in enhancing the proliferation and integration of cells. (omicsonline.org)

2006 ) Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells. (neurotree.org)
Among these is the isolation of the first line of murine stem cells [ 5 , 6 ] in 1981, followed by establishment of the first human embryonic stem cell lines by Thompson [ 7 ]. (intechopen.com)

Diminished myelin levels lead to further cell death in which unmyelinated neurons die. (karger.com)
Parkinsons Congress 2019: Derivation of dopaminergic neurons from embryonic stem cells using a silk nanofibrous scaffold- Maryam Nazm Bojnordi, Ebrahimi-Barough S, Vojoudi E and Ghasemi H- University of Medical Sciences, Iran. (alliedacademies.org)

2007 ) The effects of soluble growth factors on embryonic stem cell differentiation inside of fibrin scaffolds. (neurotree.org)
Asmani, M.N., J. Ai, G. Amoabediny, A. Noroozi, M. Azami, S. Ebrahimi-Barough, M. Navaei-Nigjeh, A. Ai, M. Jafarabadi (2013) Three-dimensional culture of differentiated endometrial stromal cells to oligodendrocyte progenitor cells (OPCs) in fibrin hydrogel. (karger.com)

Bone marrow appears to contain three stem cell populations: hematopoietic stem cells, stromal stem cells and endothelial precursor cells. (bvsalud.org)
Hematopoietic stem cells are cells isolated from blood and bone marrow that can differentiate into a variety of different specialized cells and suffer apoptosis 1 . (bvsalud.org)

The fibrocartilaginous effects of fibrochondrogenesis on chondrocytes has shown potential as a means to produce therapeutic cellular biomaterials via tissue engineering and manipulation of stem cells, specifically human embryonic stem cells. (wikipedia.org)
2019 ) Intracellular delivery of mRNA to human primary T cells with microfluidic vortex shedding. (neurotree.org)
Noninvasive detection and imaging of molecular markers in live cardiomyocytes derived from human embryonic stem cells Biophysical Journal. (nottingham.ac.uk)
Here we derived NCSCs from human iPSCs and embryonic stem cells (ESCs), and investigated the potential of NCSCs for neural tissue engineering. (ca.gov)
This platform prompted the discovery of NELL2 expression in human oligodendroglial cell types and NELL2 was further linked with human white mater development and diseases by using artificial intelligence prediction (Shaker MR et al, Frontiers in Cell and Developmental Biology, 2022). (edu.au)
This will improve our capacity to how cells arrange into a continuous elongating neural tube to provide insight into the cause of defects in human neural tube. (edu.au)
Neurotrophin-induced differentiation of human embryonic stem cells on three-dimensional polymeric scaffolds. (ucdenver.edu)
Shih CC, Forman SJ, Chu P, Slovak M. Human embryonic stem cells are prone to generate primitive, undifferentiated tumors in engrafted human fetal tissues in severe combined immunodeficient mice. (razavihospital.ir)
Teratoma formation by human embryonic stem cells is site dependent and enhanced by the presence of Matrigel. (razavihospital.ir)
17 Human hair follical stem cells: markers, selection and perspective clinic application. (vinastemcelllab.com)
Promotes self-renewal and maintains pluripotency of human embryonic stem cells and mouse Epi-stem cells when used in combination with CHIR99021 (Kim et al. (stemcell.com)
Induces the differentiation of human PSC-derived alveolar epithelial type II (AETII) to AETI cells (Ghaedi et al. (stemcell.com)
Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate. (ca.gov)
Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. (ca.gov)
We previously demonstrated that human embryonic stem cell-derived mesodermal progenitors (hES-MPs) hold a great potential to provide a homogeneous and unlimited supply of cells for bone engineering applications. (diva-portal.org)
Media of known composition that maintain human embryonic stem (hES) cells have been developed, but finding chemically defined, robust substrata has proven difficult. (nih.gov)
To test this, EPCs isolated from human umbilical cord blood or from adult peripheral blood, and human saphenous vein smooth muscle cells (HSVSMCs) as a source of perivascular cells, were combined in Matrigel and implanted subcutaneously into immunodeficient mice. (ashpublications.org)
Quantitative analyses showed the microvessel density was significantly superior to that generated by human dermal microvascular endothelial cells (HDMECs) but similar to that generated by human umbilical vein endothelial cells (HUVECs). (ashpublications.org)
Together, Biosilk and Biolaminin 521 create a biologically relevant 3D culture environment for the expansion and differentiation of human ES and iPS cells. (biolamina.com)
The laminin 521 protein is also one of the most common laminins expressed in the human adult body and therefore Biolaminin 521 also supports the culture of specialized tissue cell types, such as pancreatic cells, liver cells, vascular cells, cardiac and skeletal muscle cells, and different neural cells. (biolamina.com)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)
Finally, in situ hybridization analyses revealed the presence of RP11-295G20.2 in the nuclei of corneal epithelial cells, but not in the stromal cells of the human cornea, while RP11-322M19.1 was present in epithelial and non-epithelial cells. (molvis.org)
Electrical stimulation of human embryonic stem cells: Cardiac differentiation and the generation of reactive oxygen species. (unipd.it)
Cells harvested from the testicular sperm extraction (TESE) samples of the azoospermic patients were cultured on the 3D porous scaffolds containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs) for two weeks. (ijfs.ir)
Therefore, 3D HSA/TCP NPs scaffolds could be used for the reconstitu- tion of the artificial human somatic testicular niche for future applications in regenerative medicine for male infertility. (ijfs.ir)
Following our previous animal studies performed on mice (9, 10), the aim of this study was to evaluate the viability and proliferation of the cells derived from human TESE samples, which were cultivated on a novel threedimensional (3D) nano-scaffold containing human serum albumin (HSA)/tri calcium phosphate nanoparticles (TCP NPs), as examined by MTT and H&E histological staining assays. (ijfs.ir)

We hypothesize that blood-derived endothelial progenitor cells (EPCs) have the required proliferative and vasculogenic activity to create vascular networks in vivo. (ashpublications.org)
Using zebrafish as the main model system, we combine cutting-edge epigenomic, transcriptomic, chromosome conformation capture techniques (ChIP-seq, ATAC-seq, RNA-seq, HiC, HiChIP, UMI-4C) and single-cell technologies with functional studies using CRISPR-Cas systems, to understand these processes in an in vivo context. (cabd.es)
In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel. (unipd.it)
More recently, few clinical attempts have offered encouraging results with ex vivo or stem cell-based engineered airways and tracheal allografts implanted after heterotopic revascularization. (biomedcentral.com)
The biomaterial scaffold design should consider physicochemical properties, microstructure, and degradation in vivo . (openorthopaedicsjournal.com)

Molecular cues for pluripotency, genetic reprogramming of adult stem cells, and widely multipotent adult cells. (razavihospital.ir)
The resulting cells maintained a normal karyotype and had high levels of pluripotency markers. (nih.gov)
Moreover, in parallel, several groups have demonstrated the pluripotency of germ-line stem cells (GSCs) following SSCs culture in rodents, though, there is a debate about pluripotency of GSCs in primates and humans (5). (ijfs.ir)

My research interests focus mainly on stem cells (embryonic, 'adult' and fetal origins) and their applications in tissue engineering, particularly the osteoblast and bone tissue. (nottingham.ac.uk)
There are basically two types of stem cells: embryonic and somatic (or adult). (bvsalud.org)
These cells are classified as totipotent and they can form any of the tissue types found in the adult body, in addition to having unlimited proliferation potential 6 . (bvsalud.org)
Adult stem cells are classified as pluripotent and are undifferentiated cells that remain quiescent in tissues until stimulated, when they can create cell types that are compatible with the tissue in which they reside. (bvsalud.org)
By definition, adult stem cells are capable of differentiation into at least two lineages and have the property of self-renewal. (bvsalud.org)
Building upon this work we are exploring the differentiation of embryonic and adult stem cells for articular cartilage and bone repair. (ucdavis.edu)
The unit will demonstrate various types of stem cells: adult, embryonic, induced pluripotent and their specialised culture methods. (manchester.ac.uk)

Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. (razavihospital.ir)
Given the important role of both cytokine-mediated inflammatory microenvironment and cytolytic NK cell activities in immune-oncology therapies, our findings present a compelling argument for further clinical investigation. (frontiersin.org)

Embryonic stem cells (ESC) are both a potential source of cells for tissue replacement therapies and an accessible tool to model early embryonic development. (ox.ac.uk)

This study demonstrates that iPSC-derived multipotent NCSCs can be directly used for tissue engineering and that the approach that combines stem cells and scaffolds has tremendous potential for regenerative medicine applications. (ca.gov)
15 Cellularized scaffolds: new clothes for cardiac regenerative medicine. (vinastemcelllab.com)
Philippa Francis-West is a Professor in Developmental and Cell Biology at the Centre for Craniofacial and Regenerative Biology, King's College London. (royalsociety.org)

Embryonic stem cells (ESC) provide a great therapeutic potential for cardiac tissue. (jscimedcentral.com)

This article reviews recent advancements in biomaterial strategies for the differentiation of neural stem cells into oligodendrocytes, and presents new data indicating appropriate properties for oligodendrocyte precursor cell growth. (karger.com)
Among the current biomaterial applications, the use of biopolymers as cell scaffolds could aid the development of stem cell-based therapies aimed at replacing tissue loss due to injuries or diseases, such as during myocardial infarction. (jscimedcentral.com)

Bakshi, A., C.A. Keck, V.S. Koshkin, D.G. LeBold, R. Siman, E.Y. Snyder, T.K. McIntosh (2005) Caspase-mediated cell death predominates following engraftment of neural progenitor cells into traumatically injured rat brain. (karger.com)
5 Differentiation of periodontal stem/progenitor cells: roles of tgf-β1. (vinastemcelllab.com)
In recent years, the lab has identified stem and progenitor cells in articular cartilage, muscle and synovial tissues. (ucdavis.edu)

The regrowth of myelinating oligodendrocytes from neural stem cells through a biomaterials approach may prove to be a beneficial strategy following the onset of demyelination. (karger.com)
In conclusion, interfacing hES-MPs to EBM-fabricated scaffolds may represent an interesting strategy for design of third-generation biomaterials, with the potential to promote implant integration in clinical conditions characterized by poor bone quality. (diva-portal.org)
Explain how biomaterials can be used to fabricate 3D scaffolds. (manchester.ac.uk)

Also, stem cell therapy accompanied with scaffolds, is a promising treatment in neural tissue engineering to induce neural differentiation in damaged tissue of brain. (alliedacademies.org)

The group aims to understand the mechanisms of morphogenesis and cell differentiation within an embryo. (royalsociety.org)

Even though the integration of stem cells was promising, neural stem cell studies still have many milestones to reach. (omicsonline.org)

The current study aimed to assess the proliferation of hTCs through the cell culture on the three-dimensional (3D) porous scaffolds. (ijfs.ir)

We used an array of self-assembled monolayers to identify peptide surfaces that sustain pluripotent stem cell self-renewal. (nih.gov)
A stem cell is an undifferentiated unit with powerful self-renewal properties that is capable of organizing other cell types in the body. (bvsalud.org)
Stem cells are defined as undifferentiated cells that have precursor properties, are capable of forming many different cell types and have the property of unlimited self-renewal 5 . (bvsalud.org)

Fibrochondrogenesis alters the normal function of chondrocytes, fibroblasts, metaphyseal cells and others associated with cartilage, bone and connective tissues. (wikipedia.org)
Overwhelming disorganization of cellular processes involved in the formation of cartilage and bone (ossification), in combination with fibroblastic degeneration of these cells, developmental errors and systemic skeletal malformations describes the severity of this lethal osteochondrodysplasia. (wikipedia.org)
Fibrous Scaffolds with Varied Fiber Chemistry and Growth Factor Delivery Promote Repair in a Porcine Cartilage Defect Model. (ucdenver.edu)
Stromal cells are a mixed population that can create bone, cartilage and fat and also fibrous and connective tissues. (bvsalud.org)

Komura H, Ogita H, Ikeda W, Mizoguchi A, Miyoshi J, Takai Y. Establishment of cell polarity by afadin during the formation of embryoid bodies. (razavihospital.ir)

Patterning the mechanical properties of hydrogen silsesquioxane films using electron beam irradiation for application in mechano cell guidance THIN SOLID FILMS. (nottingham.ac.uk)
However, there is also evidence to suggest that undifferentiated cells can both sense the mechanical properties of their environment and differentiate accordingly. (ox.ac.uk)
This reflects their primary function, maintaining the mechanical stability of individual cells and epithelial tissues (Moll et al. (uni-freiburg.de)
Mechanical forces are critical for embryonic development - yet very little is known about their roles. (royalsociety.org)

Tissue-engineered nerve conduits were fabricated by seeding NCSCs into nanofibrous tubular scaffolds, and used as a bridge for transected sciatic nerves in a rat model. (ca.gov)

21 The cancer stem cell hypothesis and its impact on the design of new cancer therapies. (vinastemcelllab.com)
Natural killer cells are cytolytic lymphocytes belonging to the innate immune system and are involved in anti-viral and anti-tumor responses ( 1 ) and are recognized as major players in immune-mediated anti-tumor therapies ( 2 ). (frontiersin.org)
As such, preserving the integrity of sensitive samples is a daily challenge for cell culture laboratories in their search for innovative new therapies. (ddw-online.com)
For research purposes, autologous cell therapies are often favoured because they do not require immunological matching and are personalised to the patient. (ddw-online.com)
Immuno-oncology therapies typically use genetically modified T-cells to combat and kill cancerous tissue by identifying the cancer cells as a foreign body in need of attack. (ddw-online.com)
T-cell therapies based on chimeric antigen receptor (CAR) T-cells are widely employed for this purpose, as they have the capacity to proliferate within the patient after infusion and offer sustained functional immunity. (ddw-online.com)
These innovative therapies rely on the use of techniques and technologies that minimise the risk of contamination and achieve the strictly-controlled, secure environment necessary for growing healthy, viable cells. (ddw-online.com)
While cell therapies show great promise in bringing innovative and much needed treatments to patients, there are a number of key issues currently limiting their broader adoption. (ddw-online.com)
Safety concerns over the administration of live cells to patients, uncertainties over the way in which these therapies should be regulated, unknown downstream effects and the need for sufficient investment to fully commercialise research are all challenges that must be overcome in order to accelerate further development of cell therapies. (ddw-online.com)
The regulations surrounding cell therapies must therefore be sufficiently broad to cover this wide range of therapeutic processes, while still safeguarding product safety and quality. (ddw-online.com)
From a manufacturing point of view, autologous cell therapies can be more challenging to produce than allogeneic cell therapies, as each dose is a single batch and is derived from a different source. (ddw-online.com)

It is a lethal rhizomelic (malformations which result in short, underdeveloped limbs) form of dwarfism, exhibiting both skeletal dysplasia (malformations of bone) and fibroblastic dysplasia (abnormal development of fibroblasts, specialized cells that make up fibrous connective tissue, which plays a role in the formation of cellular structure and promotes healing of damaged tissues). (wikipedia.org)
A neuroscientist, Dr Shaker received his PhD in 2017 from Korea University, Seoul-South Korea, after working on the cellular behaviour of embryonic neural stem cells during the brain and spinal cord development as well as axial elongation. (edu.au)
Current knowledge of cellular behavior is mainly acquired by studies concerning homogenous populations of cells cultured as monolayers. (intechopen.com)
The mechanotransduction of SZP is also being studied at the cellular level, in relation to cell shape and cytoskeletal morphology. (ucdavis.edu)
Involving the direct use of whole cells or cellular material in patients, cell therapy is an umbrella term for many subdivisions of biological research, encompassing fields such as stem cell therapy, gene therapy and immunotherapy. (ddw-online.com)
8) indicates that tissue engineering might be helpful for the transplantation of germ cells by improving the cellular environment using scaffolds to enhance graft outcomes for prepubertal boys exposed to gonadotoxic treatments. (ijfs.ir)

Introduce students to various types of stem cells, their differentiation capabilities and their potential applications. (manchester.ac.uk)

In order to augment the interaction with the biological tissue, stem cells have recently been combined with metallic scaffolds for skeletal engineering applications. (diva-portal.org)

Cells Tissues Organs (2016) 202 (1-2): 85-101. (karger.com)
Additionally, they can form specialized cell types from other tissues if they are transplanted 6 . (bvsalud.org)
During animal embryonic development, gene expression must be tightly regulated in space and time to generate the plethora of cell types that compose animal tissues. (cabd.es)

Aguado, B.A., W. Mulyasasmita, J. Su, K.J. Lampe, S.C. Heilshorn (2012) Improving viability of stem cells during syringe needle flow through the design of hydrogel cell carriers. (karger.com)
Engineering synthetic hydrogel microenvironments to instruct stem cells. (ucdenver.edu)
Hydrogel microspheres increase cell survival and increase new bone volume in a gene therapy bone formation model. (biomaterials.org)

Evidence Acquisition: It is widely accepted that cells behave more natively if cultured in three-dimensional conditions. (razavihospital.ir)

12 Induced pluripotent stem cell production and characterization: an overview of somatic cell reprogramming. (vinastemcelllab.com)

By growing ESCs on flexible polydimethylsiloxane substrates with varying stiffness, we tested the hypothesis that substrate stiffness can influence ESC differentiation. (ox.ac.uk)

I cover mainly cell biology and physiology. (nottingham.ac.uk)
4 ] in 1951 cell culturing has become one of the most widely used methods with exceptional contribution to the advances in almost all fields of contemporary biology - cell biology, genetics, cell biochemistry, physiology etc. (intechopen.com)
check the tag ADOLESCENCE HN - 2008 BX - Nutrition in Adolescence FX - Adolescent Nutrition Physiology MH - Peritoneal Stomata UI - D054048 MN - A01.047.025.600.700 MN - A10.810 MS - Natural openings in the subdiaphragmatic lymphatic plexus in the PERITONEUM, delimited by adjacent mesothelial cells. (bvsalud.org)

Stem cells have attracted much interest in tissue engineering as a cell source due to their ability to proliferate in an undifferentiated state for prolonged time and capability of differentiating to different cell types after induction. (wjgnet.com)
I returned full time to Imperial College in 1999 to take up a lectureship in cell biology and tissue engineering and was also actively involved in establishing the Tissue Engineering Centre. (nottingham.ac.uk)
I was also affiliated with the Wolfson Centre for Stem Cells, Tissue Engineering and Modelling (STEM), part of the Centre for Biomolecular Sciences, which is now the Biodiscovery Institute. (nottingham.ac.uk)
After a productive and fun sabbatical spent working with Prof. Lena Kjellén at Uppsala University (2014-2015) Cathy returned to the UK to join the STEM (Stem Cells, Tissue Engineering and Modelling) Centre at the University of Nottingham. (merrylab.co.uk)
Tissue-engineered microtissue is an effective strategy for stem cell culture and therapy in nerve tissue engineering. (biomedcentral.com)
By means of scaffolds, cells, and growth factors, tissue engineering has provided enormous hope and interest in academia, industry, and the public to cure various disorders (7). (ijfs.ir)

This report demonstrates that Synthemax II-SC supports long-term culture of H9 and H14 hESC lines and permits efficient differentiation of hESCs into functional RPE. (ca.gov)

The characterization of this material was made by means of SEM, DSC, TGA, GPC, Tensile Test, Viability Assay, and stem cells behavior by qPCR and Immunofluorescence techniques. (jscimedcentral.com)

The limited capcacity of the central nervous system in repairmen of neuronal population such as dopaminergic neuron cells suggests stem cell therapy for neurogenesis in Parkinson? (alliedacademies.org)
Mora MV, Ibán MAR, Heredia JD, Laakso RB, Cuéllar R, Arranz MG. Stem cell therapy in the management of shoulder rotator cuff disorders. (wjgnet.com)
Stem cell therapy has the potential to overcome these limitations and may offer the best outcomes for treating diabetes mellitus. (vinastemcelllab.com)
Based on the pathophysiology of diabetes mellitus, stem cell therapy targets two mechanisms, namely cell replacement and immune correction. (vinastemcelllab.com)

2006). They have highly specific expression patterns, related to the epithelial type and stage of differentiation. (uni-freiburg.de)

While cell attachment was unaffected by the stiffness of the growth substrate, cell spreading and cell growth were all increased as a function of substrate stiffness. (ox.ac.uk)

Cells on Synthemax II-SC were compared with cultures grown with xenogeneic and xeno-free control substrates. (ca.gov)
The effective substrates displayed heparin-binding peptides, which can interact with cell-surface glycosaminoglycans and could be used with a defined medium to culture hES cells for more than 3 months. (nih.gov)
Similarly, several genes expressed in the primitive streak during gastrulation and implicated in early mesendoderm differentiation, such as Brachyury, Mixl1 and Eomes, were upregulated in cell cultures on stiffer compared to softer substrates. (ox.ac.uk)

2008 ) Embryonic stem cells as a platform for analyzing neural gene transcription. (neurotree.org)
2007 ) Polony analysis of gene expression in ES cells and blastocysts. (neurotree.org)
Deletion of Jarid2 leads to impaired orchestration of gene expression during cell lineage commitment. (sdbonline.org)
2 Sensitivity of hematopoietic and leukemic stem cells to hoxa gene levels. (vinastemcelllab.com)
However, the link between TADs and gene expression remains controversial and we know very little about the dynamics of TADs, E-P interactions and gene expression during cell fate decisions in differentiation and development. (cabd.es)
My goal is to shed light into the relationship between the 3D structure of the genome and gene regulation during cell fate decisions, as well as the role that lineage-determining TFs play in this connection. (cabd.es)
thus, Pnn may affect crucial alternative splicing (AS) decisions and, in turn, impact cell-type specific gene expression. (molvis.org)

Cell therapy is poised to play a pivotal role in the development of precision and personalised medicines that will transform healthcare for millions of patients worldwide. (ddw-online.com)
The meeting focused on the role of mechanobiology in multiple aspects of embryonic development. (royalsociety.org)

Neural crest stem cells (NCSCs) are multipotent and represent a valuable system to investigate iPSC differentiation and therapeutic potential. (ca.gov)

Advances in 3D non-destructive, non-invasive analysis methods and improvements in the multi-scale techniques and bioreactors to obtain test and culture 3D cell aggregates have been made. (razavihospital.ir)

Cells sense their environment through the cell membrane receptors. (ibecbarcelona.eu)
Nanoclusters of receptors can be further organized hierarchically in the cell membrane at the meso- and micro-levels to exert different biological functions. (ibecbarcelona.eu)
Drug release assays showed that Met exhibited sustained release after encapsulation by the macrophage cell membrane. (bvsalud.org)
Focal attachments have 10-15 nm distance between the cell membrane and the material surface and they are tight connections. (omicsonline.org)
Keratin filaments bundle into tonofilaments that span the cytoplasm and bind to desmosomes and other cell membrane structures (Waschke 2008). (uni-freiburg.de)
2016). Keratinocytes produce specialized proteins and lipids which are used to construct the cornified envelope (CE), a heavily crosslinked submembranous layer that confers rigidity to the upper epidermis, allows keratin filaments to attach to any location in the cell membrane (Kirfel et al. (uni-freiburg.de)

Furthermore, RPE generated on Synthemax II-SC are functional when seeded onto parylene-C scaffolds designed for clinical use. (ca.gov)
Paediatric germ cell tumours (GCT) are rare tumours and are unique because of varied clinical presentation and locations. (bvsalud.org)

Anatomy61

Organisms17

Diseases6

Chemicals and Drugs77

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes62

Disciplines and Occupations1

Technology, Industry, Agriculture9

Information Science3

Extracellular8

Vitro14

Growth and differentiation2

Proliferation and differentiation1

Mouse embryonic3

Mesenchymal11

Pluripotent stem9

Osteogenic7

Adhesion3

Hydrogels3

Regeneration10

Murine2

Neurons2

Fibrin2

Hematopoietic stem2

Human26

Vivo5

Pluripotency3

Adult7

Microenvironment2

Early embryonic1

Regenerative3

Cardiac1

Biomaterial2

Progenitor cells3

Biomaterials3

Induce1

Morphogenesis1

Neural stem1

Porous1

Self-renewal3

Cartilage4

Embryoid bodies1

Mechanical4

Nanofibrous1

Therapies11

Cellular6

Types of stem cells1

Skeletal1

Tissues3

Hydrogel3

Cultured in three-dimensional1

Somatic1

ESCs1

Physiology3

Tissue Engineering6

HESC1

Behavior1

Stem cell th4

Epithelial type1

Attachment1

Substrates3

Gene7

Development2

IPSC1

Aggregates1

Membrane6

Clinical2