• We report RNA-catalysed RNA synthesis on structured templates when using trinucleotide triphosphates (triplets) as substrates, catalysed by a general and accurate triplet polymerase ribozyme that emerged from in vitro evolution as a mutualistic RNA heterodimer. (elifesciences.org)
  • Here we report the map-based cloning of mop1 , an RNA-dependent RNA polymerase gene (RDRP), most similar to the RDRP in plants that is associated with the production of short interfering RNA (siRNA) targeting chromatin 5 , 6 . (nature.com)
  • Mutations causing amyotrophic lateral sclerosis (ALS) often affect the condensation properties of RNA-binding proteins (RBPs). (crick.ac.uk)
  • However, some scientists think that before life adopted DNA and proteins, it relied primarily on RNA. (elifesciences.org)
  • Yet, some RNA strands can also fold to form ribozymes, 3D structures that could have guided life's chemical processes the way proteins do now. (elifesciences.org)
  • All life on Earth today uses a triplet RNA code to make proteins from DNA, and these experiments showed how RNA triplets might have helped RNA sustain early life forms. (elifesciences.org)
  • The replicase proteins p33 and p92 of Cymbidium ringspot virus (CymRSV) were found to support the replication of defective interfering (DI) RNA in Saccharomyces cerevisiae cells. (microbiologyresearch.org)
  • His fields of research comprise RNA-mediated gene silencing processes with a focus on epigenetic phenomena, including studies on RNA-directed DNA methylation, the characterization of virus silencing suppressor proteins, the development of plant bioreactor platforms and viroid research. (degruyter.com)
  • Viroids are non-encapsidated, single-stranded (ss), 250-400 nucleotide (nt)-long circular RNA molecules that do not encode proteins (1, 2). (degruyter.com)
  • We have all learned the central dogma of molecular biology: the DNA code is translated to messenger RNA which, in turn, is transcribed to proteins. (cancernetwork.com)
  • QIAGEN offers a broad range of consumable kits, instruments and bioinformatics to study DNA, RNA and proteins. (eacr.org)
  • Our theory explains, energetically, why a truncated RNA is encapsidated by STMV capsid proteins more favorably. (escholarship.org)
  • More than 240 million people throughout the world are chronically infected with hepatitis B virus (HBV), and approximately 15-25 million are co-infected with hepatitis D virus (HDV), a satellite virus which requires HBV envelope proteins for particle assembly and spread [1] . (researchsquare.com)
  • Similar results were obtained with sucrose density gradient fractions containing RNA molecules of the size expected for ssRNA-3. (microbiologyresearch.org)
  • Small RNAs have proven to be essential regulatory molecules encoded within eukaryotic genomes. (biomedcentral.com)
  • The replication of cymbidium ringspot tombusvirus defective interfering-satellite RNA hybrid molecules. (microbiologyresearch.org)
  • At least in plants, DNA regions that become de novo methylated can be defined by homologous RNA molecules in a process termed RNA-directed DNA methylation (RdDM). (degruyter.com)
  • Upon inoculation, mature Pospiviroidae RNA molecules enter the plant cell nucleus. (degruyter.com)
  • This reservoir has dramatically increased in the past few years and it now contains over 1700 tertiary RNA structures, both, in the form of isolated molecules as well as RNA-protein and ligand complexes. (biomedcentral.com)
  • Labelling with BrUTP showed that peroxisomes were the sites of nascent viral synthesis, whereas in situ hybridization indicated that DI RNA progeny were diffused throughout the cytoplasm. (microbiologyresearch.org)
  • Using mean-field theory, we explore the role of the secondary structure of RNA on the viral assembly by modeling the RNA as an annealed branched polymer. (escholarship.org)
  • The HDV virions possess an outer layer containing hepatitis B surface antigens (HBsAg) and host lipid surrounding the inner nucleocapsid that consists of viral RNA. (researchsquare.com)
  • 1,000 HIV-1 RNA copies/mL) and with unsuppressed viral load (viremic), the prevalence of unsuppressed HIV (population viremia), sex-specific HIV transmission ratios (number female incident HIV-1 infections/number unsuppressed male PLHIV per 100 persons-years [PY] and vice versa) and examined correlations between a variety of VL metrics and incident HIV. (cdc.gov)
  • The bamboo mosaic virus satellite RNA cis-regulatory element is an RNA element found in the 5' UTR of the genome of the bamboo mosaic virus. (wikipedia.org)
  • These short RNAs participate in a diverse array of cellular processes including gene regulation, chromatin dynamics and genome defense. (biomedcentral.com)
  • As part of the genome sequencing initiative for the tammar, we have explored the evolution of each of the major classes of mammalian small RNAs in an Australian marsupial for the first time, including the first genome-scale analysis of the newest class of small RNAs, centromere repeat associated short interacting RNAs (crasiRNAs). (biomedcentral.com)
  • We examined each of these small RNA classes with respect to the newly assembled tammar wallaby genome for gene and repeat features, salient features that define their canonical sequences, and the constitution of both highly conserved and species-specific members. (biomedcentral.com)
  • This study encompasses the first analyses of the major classes of small RNAs for the newly completed tammar genome, validates preliminary annotations using deep sequencing and computational approaches, and provides a foundation for future work on tammar-specific as well as conserved, but previously unknown small RNA progenitors and targets identified herein. (biomedcentral.com)
  • Small RNAs play important roles in many aspects of pre- and post-transcriptional gene regulation, epigenetic modifications, chromosome segregation and genome structure. (biomedcentral.com)
  • Our results verify that RNA branchedness maximizes the amount of encapsulated genome into a relatively small capsid and makes assembly more efficient. (escholarship.org)
  • In somatic cells, mitotic transcription of major satellite non-coding RNAs is tightly regulated and essential for heterochromatin formation and the maintenance of genome integrity. (bvsalud.org)
  • The recruitment of this machinery results in the production of viroid-derived small RNAs (vd-sRNAs) that mediate RNA degradation and DNA methylation of cognate sequences. (degruyter.com)
  • The RNA FRABASE 2.0 search engine algorithms operate on the database of the RNA sequences and the new library of RNA secondary structures, coded in the dot-bracket format extended to hold multi-stranded structures and to cover residues whose coordinates are missing in the PDB files. (biomedcentral.com)
  • An intuitionally operated web server platform enables very fast user-tailored search of three-dimensional RNA fragments, their multi-parameter conformational analysis and visualization. (biomedcentral.com)
  • RNA FRABASE 2.0 also stores data and conformational parameters in order to provide "on the spot" structural filters to explore the three-dimensional RNA structures. (biomedcentral.com)
  • One of the most difficult issues in mining available structural data is the search of RNA structural motifs and fragments in a conformational space. (biomedcentral.com)
  • Post-transcriptional steps of gene expression (pre-mRNA splicing, polyadenylation, stability, translation, localization and modifications) as well as the functions of non-coding RNAs are increasingly diverse. (rnasociety.org)
  • The characterization of new miRNA target genes and a unique profile for crasiRNAs has allowed for insight into multiple RNA mediated processes in the tammar, including gene regulation, species incompatibilities, centromere and chromosome function. (biomedcentral.com)
  • The first class of small RNAs identified were the micro RNAs (miRNAs), which are small (~22 nt) non-coding RNAs that regulate gene expression by base pairing to mRNAs where they direct either mRNA cleavage or repress translation [ 8 ]. (biomedcentral.com)
  • The fate of yeast and plant DNA, as monitored by yeast chitinase gene and the plant chlorophyll a/b binding protein gene and micro-satellite markers showed that large DNA markers were present in must (the starting fermentation mixture of mainly yeast and grape juice), while the 250 base pair micro-satellites were present in both must and young wine for up to six months [9]. (i-sis.org.uk)
  • Gene expression analysis at the bulk and single-cell level revealed that these cells are distinct from Itga7-expressing muscle satellite cells. (jci.org)
  • A satellite-like nucleic acid of arabis mosaic virus associated with hop nettlehead disease. (microbiologyresearch.org)
  • How much yeast nucleic acid (DNA and RNA) is carried over into wine? (i-sis.org.uk)
  • Here, we use validated locked nucleic acid gapmers for major satellite RNA depletion followed by live cell imaging, and superresolution analysis to determine the role of pericentric non-coding RNAs during female meiosis. (bvsalud.org)
  • Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
  • Depletion of satellite RNA induces mesoscale changes in pericentric heterochromatin structure leading to chromosome instability, kinetochore attachment errors and abnormal chromosome alignment. (bvsalud.org)
  • However, the role of RBP condensation in the specificity and function of protein-RNA complexes remains unclear. (crick.ac.uk)
  • Satellites of plant viruses. (microbiologyresearch.org)
  • College of Agriculture and Life Sciences, Seoul National University, Korea): Use of high-density latex agglutination test for detection of plant viruses Tien Po (Institute of Microbiology, CAS): Plant Virus Satellite RNA and its roles in Plant Virus Disease Control (Temporary) Tang Jiliang (Guanxi Agricultural University): The Pathogenesis of Bacterial Plant Pathogens Fang Rongxiang (Institute of Microbiology, CAS): Molecular Aspects of Rice Virus The plenary lectures may be changed in case. (bio.net)
  • Two yeast strains were used, differing in the biogenesis of peroxisomes, the organelles supplying the membranous vesicular environment in which CymRSV RNA replication takes place in infected plant cells. (microbiologyresearch.org)
  • DI RNA replication occurred in yeast cells, as demonstrated by the presence of monomers and dimers of positive and negative polarities. (microbiologyresearch.org)
  • DI RNA replication also took place in yeast cells devoid of peroxisomes. (microbiologyresearch.org)
  • To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
  • Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)
  • When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
  • Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
  • When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)
  • Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
  • MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
  • The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
  • Satellite cells, the engines of muscle repair. (scielo.br)
  • Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
  • Günther S, Kim J, Kostin S, Lepper C, Fan CM, Braun T. Myf5-positive satellite cells contribute to Pax7-dependent long-term maintenance of adult muscle stem cells. (scielo.br)
  • In the United States, a Harvard professor, Derrick J. Rossi, PhD, found these mRNA congeners could reprogram stem cells, and this led to founding of Moderna ("Modified RNA"), in conjunction with the Massachusetts Institute of Technology investigators and entrepreneurial funding. (cancernetwork.com)
  • Skeletal muscle satellite cells (SC) play an important role in muscle repair following injury. (mcmaster.ca)
  • Previous studies have derived MRF expression from whole muscle homogenates, but little work has examined whether the mRNA expression of these transcripts reflects the pattern of expression in the actual population of satellite cells (SC). (mcmaster.ca)
  • From the biopsies, muscle stem cells (satellite cells) are isolated and later used for preclinical measurements. (lu.se)
  • Our exercise studies include muscle biopsies and skeletal muscle satellite cells. (lu.se)
  • Presumably, this process involves the interaction between the host's bromodomain-containing viroid RNA-binding protein 1 (VIRP1) and the viroid's TR domain (6). (degruyter.com)
  • We offer gold-standard extraction kits, assays and bioinformatics for CTCs , exosomes and cell-free DNA / RNA . (eacr.org)
  • This element is thought to be essential for efficient RNA replication. (wikipedia.org)
  • RNA-catalyzed RNA replication is widely believed to have supported a primordial biology. (elifesciences.org)
  • However, RNA catalysis is dependent upon RNA folding, and this yields structures that can block replication of such RNAs. (elifesciences.org)
  • To address this apparent paradox, we have re-examined the building blocks used for RNA replication. (elifesciences.org)
  • The triplets cooperatively invaded and unraveled even highly stable RNA secondary structures, and support non-canonical primer-free and bidirectional modes of RNA synthesis and replication. (elifesciences.org)
  • Triplet substrates thus resolve a central incongruity of RNA replication, and here allow the ribozyme to synthesise its own catalytic subunit '+' and '-' strands in segments and assemble them into a new active ribozyme. (elifesciences.org)
  • A blowout in Ohio made little news in 2018, but satellite images show it was a major global event. (livescience.com)
  • The satellite] observed the blowout emission on 27 February, 2018, which was the 13th day in the blowout period, which likely does not represent the peak emission rate," the researchers wrote in the paper. (livescience.com)
  • RNA silencing genes control de novo DNA methylation. (nature.com)
  • Since then, he has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and his main field of interest is RNA-interference (RNAi) and RNA-directed DNA methylation (RdDM) in plants. (degruyter.com)
  • For example, these triplets work together to bind tightly to RNA strands and unravel structures that block RNA copying. (elifesciences.org)
  • Just as Google maps have a satellite view, a traffic view, a street view - we can create a "Google medical map" of a human being from external features to anatomy (by scans) to physiology (by sensors), to DNA, RNA and chemical composition. (sas.com)
  • In this respect, a deeper knowledge of complex three-dimensional RNA structures is essential to understand their new biological functions. (biomedcentral.com)
  • A number of bioinformatic tools have been proposed to explore two major structural databases (PDB, NDB) in order to analyze various aspects of RNA tertiary structures. (biomedcentral.com)
  • One of these tools is RNA FRABASE 1.0, the first web-accessible database with an engine for automatic search of 3D fragments within PDB-derived RNA structures. (biomedcentral.com)
  • RNA FRABASE 2.0 has stored information on 1565 PDB-deposited RNA structures, including all NMR models. (biomedcentral.com)
  • An instant visualization of the 3D RNA structures is provided. (biomedcentral.com)
  • A deep knowledge of complex three-dimensional folds of RNA structures is essential to understand the range of their biological functions. (biomedcentral.com)
  • The first program calculates a complete set of rigid-body parameters that describe the detailed geometry of double helices extracted from the tertiary RNA structures. (biomedcentral.com)
  • Accumulating evidence supports the importance of RNA biology in the hallmarks of cancer. (rnasociety.org)
  • The internationally renowned experts invited to this symposium will present the recent advances in our understanding of RNA biology in cancer. (rnasociety.org)
  • Since then, she has been working as a postodoc in Dr. Wassenegger's lab (RLP AgroScience GmbH, AlPlanta) and her main field of interest is RNA-interference (RNAi) and viroid biology in plants. (degruyter.com)
  • Notably, a capacity for condensation was required for efficient TDP-43 assembly on subsets of RNA-binding regions, which contain unusually long clusters of motifs of characteristic types and density. (crick.ac.uk)
  • We establish that RBP condensation can occur in a binding-region-specific manner to selectively modulate transcriptome-wide RNA regulation, which has implications for remodeling RNA networks in the context of signaling, disease, and evolution. (crick.ac.uk)
  • When RNA is copied, one strand acts as a template, and a replicase ribozyme would accurately guide which letters are added to the strand under construction. (elifesciences.org)
  • Such allodynia was associated with elevation of markers for activated microglia/macrophages (CD11b) and astrocytes/satellite glia (GFAP) in the lumbar dorsal spinal cord and dorsal root ganglia (DRG). (cdc.gov)
  • This Microsymposium is a three-day international conference to discuss their latest findings in the exciting field of small RNAs. (rnasociety.org)
  • Swarms of small satellites could communicate with one another to collect data on important weather patterns at different times of the day or year, and from multiple angles. (scitechdaily.com)
  • However, a small satellite covering a large area cannot make high spatial resolution observations. (scitechdaily.com)
  • Genetic and functional diversification of small RNA pathways in plants. (nature.com)
  • Herr, A. J. Pathways through the small RNA world of plants. (nature.com)
  • Using next generation sequencing, we have characterized the major classes of small RNAs, micro (mi) RNAs, piwi interacting (pi) RNAs, and the centromere repeat associated short interacting (crasi) RNAs in the tammar. (biomedcentral.com)
  • A survey of the tammar gonadal piRNAs shows that these small RNAs are enriched in retroelements and carry members from both marsupial and tammar-specific repeat classes. (biomedcentral.com)
  • These small RNAs are derived largely from centromere-enriched retroelements, including a novel SINE. (biomedcentral.com)
  • Each class of small RNAs is synthesized by a distinct mechanism and each has discrete biological functions. (biomedcentral.com)
  • Another class of important small RNAs is the piRNAs. (biomedcentral.com)
  • Of course, Christy's comparisons between models and satellite data are always apples-to-apples, so the small influence of the stratosphere on the MT channel is included in both satellite and climate model data. (drroyspencer.com)
  • Inhibition of transcription or RNA splicing phenocopies the chromosome alignment errors and spindle defects, suggesting that pericentric transcription during oocyte meiosis is required to regulate heterochromatin structure, chromosome segregation and maintenance of spindle organization. (bvsalud.org)
  • Quantitative determination of HDV RNA can be used to monitor the response of antiviral therapy and to determine HDV genotype by sequencing HDV RNA positive samples. (researchsquare.com)
  • For early life to be built on RNA, though, this molecule must have had the ability to make copies of itself. (elifesciences.org)
  • Biochemical techniques were used to engineer a ribozyme that copies RNA strands by adding letters not one-by-one, but three-by-three. (elifesciences.org)
  • NASA-NOAA's Suomi NPP satellite passed over Tropical Depression Nora on October 14 and saw strong wind shear pushing the bulk of clouds and showers northeast of the center. (phys.org)
  • The Suomi NPP satellite is co-managed by both NASA and NOAA. (phys.org)
  • These new images come straight from the Suomi NPP weather satellite, which is equipped with the Visible Infrared Imaging Radiometer Suite. (iflscience.com)
  • 2014) A bromodomain-containing host protein mediates the nuclear importation of a satellite RNA of Cucumber mosaic virus. (forth.gr)
  • From new RNA delivery mechanisms, to managing clinical trials of Lecanemab, hailed as a "historic moment" in Alzheimers treatment. (ukspa.org.uk)
  • The second one finds all base pairs and multiplets in the RNA structure and offers the classification of canonical and non-canonical base-pairs. (biomedcentral.com)
  • Even when we use the latest satellite datasets used by Santer, these are the results which back up my testimony. (drroyspencer.com)
  • But it wasn't clear how large the leak was until researchers in the new paper, studying data from a new European Space Agency (ESA) methane-monitoring satellite, spotted the plume. (livescience.com)
  • The sheer scale of the Ohio incident might have gone unnoticed if the team of Dutch and U.S. researchers behind this paper hadn't decided to specifically look for the leak in the satellite data after hearing reports of the blowout, the Times wrote. (livescience.com)
  • In 2016, for example, NASA used the satellite to gather data on power outages for federal authorities. (iflscience.com)
  • Like DNA, strands of RNA contain genetic data. (elifesciences.org)
  • In a recently published paper in J. Climate entitled Comparing Tropospheric Warming in Climate Models and Satellite Data , Santer et al. (drroyspencer.com)
  • use a combination of lesser-known satellite datasets and neglect of radiosonde data to reduce the model bias to only 1.7 times too much warming. (drroyspencer.com)
  • twist themselves into a pretzel by cherry picking data, using a new hot satellite dataset that appears to be undocumented, ignores independent (radiosonde) evidence (since it does not support their desired conclusion), and still arrives at a substantial 1.7x average bias in the climate models warming rates. (drroyspencer.com)
  • RNA FRABASE 2.0 provides a novel database and powerful search engine which is equipped with new data and functionalities that are unavailable elsewhere. (biomedcentral.com)
  • These tools will be applied to 2 years of GEO ephemeris data of active satellites at DF&NN. (sbir.gov)
  • Individuals attending RNA2017 may also be interested in attending these satellite meetings, sponsored by the RNA Society, to be held before or after the RNA meeting, in or near Prague. (rnasociety.org)
  • SIG and Satellite Meetings will be held during the two days prior to the main conference: Friday and Saturday, July 8 and July 9. (iscb.org)
  • NASA-NOAA's Suomi satellite flew over Nora at 12:15 UTC (8:15 a.m. (phys.org)
  • To download a certificate of analysis for chicory yellow mottle nepovirus with satellite RNA ( PV-577 ), enter the lot number exactly as it appears on your product label or packing slip. (atcc.org)
  • The certificate of analysis for that lot of chicory yellow mottle nepovirus with satellite RNA ( PV-577 ) is not currently available online. (atcc.org)
  • The latest University of Wisconsin- Cooperative Institute for Meteorological Satellite Studies vertical wind shear analysis showed 44 knots (50.6 mph / 81.4 kph) of wind shear from the southwest and ships guidance showed 34 knots (39.1 mph/62.9 kph) of shear from the same direction. (phys.org)
  • Itga7 + cell heterogeneity revealed by single-cell RNA-Seq analysis. (jci.org)
  • The advent of remote methane-sensing equipment on ESA satellites is offering researchers a new window onto the problem. (livescience.com)
  • Improvements in satellite monitoring might help researchers get a better handle on the issue, the researchers added. (livescience.com)
  • Our intention is that this advanced version of the RNA FRABASE will be of interest to all researchers working in the RNA field. (biomedcentral.com)
  • We invite you to submit a proposal to organize a Special Interest Group (SIG) or Satellite Meeting (SM) at ISMB 2016 conference, which will be held at the Swan and Dolphin Hotel, Orlando, United States, July 8 and 9, 2016. (iscb.org)