Satellite cells in adult muscles skeletal muscle cells progenitor satellite cell. Medical search. Frequent questions

Cellular and molecular regulation of muscle regeneration. (scielo.br)
Satellite cells are essential for skeletal muscle regeneration:the cell on the edge returns centre stage. (scielo.br)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
After a second round of injury, muscle regeneration is impaired in C/EBPβ conditional knockout mice compared to wild-type control mice. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
Further, C/EBPβ-deficient SCs are unable to self-renew after muscle injury leading to a reduction in the SC pool such that it cannot efficiently support muscle regeneration after a second round of injury. (biomedcentral.com)
Regeneration of muscle after injury normally occurs rapidly with satellite stem cells that reside in the space between the basal lamina and the sarcolemma, playing a major role [ 11 ]. (hindawi.com)
Skeletal muscle regeneration is regulated by coordinated activation of multiple signaling pathways. (elifesciences.org)
However, the role of individual arms of the UPR in skeletal muscle regeneration remain less understood. (elifesciences.org)
Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
However, the mechanisms of muscle regeneration are not yet fully understood. (elifesciences.org)
Stem cells are considered the most promising source for such meat, retaining as they do the capacity to transform into the required tissues, and the scientists pointed to satellite cells, which are the natural muscle stem cells responsible for regeneration and repair in adults. (livescience.com)
in vivo proliferation of these satellite cells will then contribute to repeated regeneration and further therapeutic effect by increasing the amount of healthy muscle fiber.To treat a muscular disease which is caused by the defect of interstitial protein, we aim to achieve therapeutic effect by supplementing the insufficient protein in the interstitial tissue through transplant of mesenchymal stromal cells generated from the iPS Cell Stock. (kyoto-u.ac.jp)
In particular, her work has been focusing on the molecular mechanisms involved in the modulation of the peripheral synaptic transmission, skeletal muscle adaptation and regeneration in physiological and pathological conditions. (units.it)
The research activity of the group is focused on the identification of new strategies to improve skeletal muscle regeneration. (units.it)
Direct isolation of satellite cells for skeletal muscle regeneration. (medecinesciences.org)
Alameddine HS, Dehaupas M, Fardeau M. Regeneration of skeletal muscle fibers from autologous satellite cells multiplied in vitro. (medecinesciences.org)
Identification of a novel population of muscle stem cells in mice: potential for muscle regeneration. (medecinesciences.org)
In this study, we investigate the effects of neural cell integration into the bioprinted skeletal muscle construct to accelerate functional muscle regeneration in vivo. (nature.com)
20% of the original mass invariably results in functional impairment with limited regeneration capacity, which requires reconstructive surgical procedures such as autologous muscle flap transfers 1 . (nature.com)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)
Defective muscle regeneration can severely affect re-innervation by motor axons, and neuromuscular junctions (NMJs) development, ultimately leading to skeletal muscle atrophy. (biomed.news)
In particular, in skeletal muscle, specific semaphorin molecules are involved in the restoration and remodeling of the nerve-muscle connections, thus emphasizing their plausible role to ensure the success of muscle regeneration. (biomed.news)
This review article aims to discuss the impact of aging on skeletal muscle regeneration and NMJs remodeling and will highlight the most recent insights about the role of semaphorins in this context. (biomed.news)

Gros J, Manceau M, Thomé V, Marcelle C. A common somitic origin for embryonic muscle progenitors and satellite cells. (scielo.br)
Embryonic myogenic progenitor cells express CXCR4, G-protein coupled cell surface receptor, and migrate towards regions of SDF-1 expression during limb muscle development, suggesting that CXCR4/SDF-1 plays a role in muscle cell homing. (biomedcentral.com)
Embryonic stem cells could also be used, but they are obviously plagued by ethical concerns, and they could grow into tissues besides the desired muscles. (livescience.com)
As constitutive knockout of Lkb1 in mice leads to embryonic lethality, whether Lkb1 is required for the growth and survival of adult mice is unclear. (omicsdi.org)
In Drosophila , a population of muscle-committed stem-like cells called adult muscle precursors (AMPs) keeps an undifferentiated and quiescent state during embryonic life. (biologists.com)
The embryonic AMPs are at the origin of all adult fly muscles and, as we demonstrate here, they express repressors of myogenic differentiation and targets of the Notch pathway known to be involved in muscle cell stemness. (biologists.com)
In this project, the group intends to investigate the nature and the role of adenosine and AR signalling pathways on the two isoforms of muscle AChRs: the embryonic (g-AChR) regulating synaptogenesis and muscle development and the adult (e-AChR) with a role in nerve-muscle communication. (units.it)

The proliferation and differentiation of satellite cells are regulated by a number of extracellular signals ( Wang & Rudnicki, 2012 Wang YX, Rudnicki MA. (scielo.br)
The balance between differentiation and self-renewal is critical to maintain muscle tissue homeostasis, and alterations in this equilibrium can lead to chronic muscle degeneration. (biomedcentral.com)
The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Researchers at the Fujian Provincial Research Institution of Traditional Chinese Medicine conclude that acupuncture reduces muscle cell death while promoting the proliferation and differentiation of muscle satellite cells. (acupunctureofiowa.com)
A number of different local transcription factors called myogenic regulatory factors (MRFs), including MyoD, myf-5, myogenin, and myf-6, stimulate proliferation and differentiation of satellite cells [ 9 ]. (hindawi.com)
In addition, insulin, such as growth factor (IGF) 1Ea and/or IGF-1Ec (MGF), also stimulates proliferation and differentiation of satellite cells. (hindawi.com)
We make research on how to induce highly efficient differentiation of adult skeletal muscles. (kyoto-u.ac.jp)
We also investigate the possibility to reproduce the disease state of muscular dystrophy by culturing differentiation-induced skeletal muscle with the physical stress culturing system. (kyoto-u.ac.jp)
Fracture Healing in Diabetes Fracture repair entails recruitment of mesenchymal stem cells after which differentiation to chondrocytes that type cartilage (see Chapter 12). (dnahelix.com)
All cells have been tested for myotube differentiation. (zen-bio.com)
Human skeletal myoblast are differentiated using Skeletal Muscle Differentiation Medium (cat# SKM-D) for at least 6 days. (zen-bio.com)
Specially formulated media are available for maintenance and differentiation of our adult skeletal myoblasts. (zen-bio.com)

Satellite cells help to repair and regenerate muscle fibers. (acupunctureofiowa.com)
Further compounding the loss in function with age is preferential loss of cross-sectional area of stronger and faster contracting type II muscle fibers [ 9 ]. (hindawi.com)
Diprotin A stimulation of CXCR4, however, significantly increased the number of canine dystrophin-positive muscle fibers and canine-derived satellite cells by enhancing donor cell proliferation. (biomedcentral.com)
As cell transplant therapy for muscular dystrophy, we envisage regenerating and repairing damaged muscle fibers by transplanting skeletal muscle stem cells generated from our iPS Cell Stock for Regenerative Medicine. (kyoto-u.ac.jp)
Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro. (medecinesciences.org)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

Acupuncture is effective for the alleviation of denervated skeletal muscle atrophy. (acupunctureofiowa.com)
The results of the aforementioned research indicates that acupuncture is an effective treatment modality for skeletal muscle atrophy. (acupunctureofiowa.com)
Effect of electroacupuncture on denervated skeletal muscle atrophy and expression of IGF-1/PI3K/AKT in rats [J]. Chinese Acupuncture and Moxibustion, 2018,38(12):1311-1317. (acupunctureofiowa.com)
Experimental study of rat denervated skeletal muscle atrophy [J]. Journal of Shanghai Medical University, 1999, 26(4): 265-268. (acupunctureofiowa.com)
Since myostatin and follistatin bind to the same receptor, the follistatin to myostatin (F:M) ratio is associated with muscle atrophy or hypertrophy [ 12 ]. (biomedcentral.com)

Skeletal muscle progenitors (satellite cells), murine cell line and immortalized human myoblasts are studied in vitro to identify, in a controlled environment, the pathways regulating the regenerative potential of the skeletal muscle. (units.it)
Transplanted myoblasts can migrate several millimeters to fuse with damaged myofibers in nonhuman primate skeletal muscle. (medecinesciences.org)
Human primary cultured skeletal myoblasts can directly reflect a patient's metabolic phenotype, because many of the signaling pathways are maintained intact. (zen-bio.com)
ZenBio offers human primary skeletal myoblasts from a variety of donors, including obese donors with Type 2 diabetes. (zen-bio.com)
Human skeletal myoblasts satellite cells are isolated from consented patients with good representation from all levels of adiposity and age. (zen-bio.com)
Skeletal myoblasts are available from both male and female donors. (zen-bio.com)

The Q+ and Q- isoforms of PAX3 are generally co-expressed in cells. (wikipedia.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

To determine their possible roles in tissues and satellite cells in vitro, their expression pattern was examined in tissues from 40-day-old chickens and in satellite cells from the breast muscles of 1-week-old and 2-week-old chickens using RT-PCR and immunocytochemistry analyses. (scielo.br)
Regarding the latter, we use iPS cells established from somatic cells taken from patients as a tool for drug discovery, by making study on the model construction in order to reproduce disease states in vitro. (kyoto-u.ac.jp)
Neural input into this bioprinted skeletal muscle construct shows the improvement of myofiber formation, long-term survival, and neuromuscular junction formation in vitro. (nature.com)
These approaches have achieved bioengineered skeletal muscle tissue fabrication with cellular alignment in vitro, and some showed a degree of therapeutic potential in vivo. (nature.com)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

Upon increasing culture times in chicken´s primary skeletal muscle satellite cells, SULF1 and SULF2 expression in 1-week-old chickens was significantly higher than in 2-week-old chickens, suggesting that sulfatases play a key role in satellite cell development. (scielo.br)

In more detail, the team is studying the role of electrical activity and local trophic factors in the microenvironment of satellite cell niche and in the post-mitotic myogenesis. (units.it)

Cell Stem Cell 2013;13(5):590-601. (scielo.br)
Therefore, these results establish the efficacy of diprotin A in stimulating muscle cell engraftment, and highlight the pre-clinical utility of a xenotransplantation model in assessing the relative efficacy of muscle stem cell populations. (biomedcentral.com)
We used a clinically acceptable regimen of hematopoietic stem cell transplantation to establish mixed donor/host blood cell chimerism and immune tolerance in a canine model of Duchenne muscular dystrophy ( cxmd ) [ 9 ]. (biomedcentral.com)
Stem cell function, self-renewal, and behavioral heterogeneity of cells from the adult muscle satellite cell niche. (medecinesciences.org)
The rapid advance of stem cell clinical trials for a broad spectrum of conditions warrants an update of the review by Trounson (2009) [ 1 ]. (biomedcentral.com)
There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)

Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
Morgan and Partridge note that skeletal muscle satellite cells "are normally quiescent in adult muscle, but act as a reserve population of cells, able to proliferate in response to injury and give rise to regenerated muscle and to more satellite cells. (acupunctureofiowa.com)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Both miRNAs and siRNAs need to be delivered into the target tissue or cell in order to activate the desired therapeutic effect," Muthiah Manoharan, Ph.D., senior vp, drug discovery, Alnylam Pharmaceuticals, explained. (genengnews.com)
The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
Skeletal muscle, the most abundant tissue of the body, has remarkable regenerative capacity mainly due to its resident muscle stem cells, also known as satellite cells. (elifesciences.org)
To grow meat in labs from satellite cells, the researchers suggested current tissue-engineering techniques, where stem cells are often embedded in synthetic three-dimensional biodegradable matrixes that can present the chemical and physical environments that cells need to develop properly. (livescience.com)
These results demonstrate that Lkb1 plays an important role in maintaining body weight, liver and adipose tissue function, blood glucose homeostasis and survival in adult mice. (omicsdi.org)
A beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion in mice. (omicsdi.org)
The results support the concept that fuel excess can drive obesity and diabetes via hyperinsulinaemia, and that an islet beta cell ATGL-lipolysis/adipose tissue axis controls energy homeostasis and body weight via insulin secretion. (omicsdi.org)
She has been reviewer for international Journals ( FASEB J , Cell & Tissue Res , Toxicon, Exp Cell Res ), for national grant agencies (MIUR) and international Foundations (AFM, Association Francaise contre les Myopathies). (units.it)
With ageing, skeletal muscle undergoes a severe reduction in tissue mass, leading to a decrease in strength (sarcopenia). (units.it)
More importantly, the bioprinted constructs with neural cell integration facilitate rapid innervation and mature into organized muscle tissue that restores normal muscle weight and function in a rodent model of muscle defect injury. (nature.com)
Skeletal muscle is a complex tissue composed of thousands of individual multinucleated myofibers. (arvojournals.org)
These kind of medical grade honey examines are usually mostly tissue-or population-based, while the specific connection is from cell-to-cell. (mrt67307inhibitor.com)
The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)
These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)

In murine-to-murine transplantation experiments, CXCR4 expression marks a population of adult murine satellite cells with robust engraftment potential in mdx mice, and CXCR4-positive murine muscle-derived SP cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice. (biomedcentral.com)
In this study, we used a canine-to-murine xenotransplantation model to quantitatively compare canine muscle cell engraftment, and test the most effective cell population and modulating factor in a canine model of DMD using allogeneic transplantation experiments. (biomedcentral.com)
Furthermore, the canine-to-murine xenotransplantation model accurately predicted results in canine-to-canine muscle cell transplantation. (biomedcentral.com)
Yet, it remains unclear from murine transplantation experiments which cell population most effectively engrafts into diseased skeletal muscle. (biomedcentral.com)

Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells. (medecinesciences.org)

When skeletal muscle is injured due to physical or chemical insult, a pool of self-renewing muscle stem cells residing within the skeletal muscles, called satellite cells, can give rise to differentiated myofibers to repair injured muscle ( Charge & Rudinicki, 2004 Chargé SBP, Rudnicki MA. (scielo.br)
Isolation and culture of individual myofibers and their satellite cells from adult skeletal muscle. (scielo.br)
We previously showed that bioprinted human skeletal muscle constructs were able to form multi-layered bundles with aligned myofibers. (nature.com)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)
The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)

Mesodermal iPSC-derived progenitor cells functionally regenerate cardiac and skeletal muscle. (medecinesciences.org)
Skeletal muscle is characterized by a remarkable capacity to rearrange after physiological changes and efficiently regenerate. (biomed.news)

Inducible depletion of satellite cells in adult, sedentary mice impairs muscle regenerative capacity without affecting sarcopenia. (scielo.br)
These adverse changes in muscle quantity, muscle quality, and the resulting functional deficits are referred to under a common name of "sarcopenia. (hindawi.com)
4 ] used the relative skeletal muscle mass index which is calculated by normalizing appendicular skeletal muscle mass, measured by dual-energy X-ray absorptiometry, to height squared in meters to diagnose sarcopenia. (hindawi.com)
The European Working Group on Sarcopenia in Older People (EWGSOP) recommends diagnosis of sarcopenia based on the presence of low muscle mass and low muscle strength or low physical performance [ 5 ]. (hindawi.com)
For the purposes of this paper, we define sarcopenia as originally defined by Rosenberg [ 3 ], an age-related loss of muscle, and view the functional aspects of the most recent definitions as consequences of sarcopenia. (hindawi.com)
Significant loss of muscle mass may occur in cachexia and sarcopenia, which are major causes of mortality and disability. (mdpi.com)
However, during aging, extensive injury, or pathological conditions, the complete regenerative program is severely affected, with a progressive loss of muscle mass and function, a condition known as sarcopenia. (biomed.news)

Among the PAX3 target genes, there is one group associated with muscle development and a second group associated with neural and melanocyte development. (wikipedia.org)
During development, one of the major lineages expressing Pax3 is the skeletal muscle lineage. (wikipedia.org)

Cell Metabolism 2013;17(2):210-24. (scielo.br)
Liver kinase B1 (LKB1) plays pivotal roles in cellular processes such as apoptosis, metabolism, and cell cycle regulation. (omicsdi.org)
Myokines are peptides or proteins released from skeletal muscle and are involved in multiple physiological processes, including metabolism and hypertrophy in the autocrine and paracrine manner [ 6 ]. (biomedcentral.com)

Also, a vascularization strategy using co-culture with endothelial cells (ECs) and fibroblasts improved the survival of the bioengineered skeletal muscle tissues 20 , 21 . (nature.com)
Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)

MicroRNA-133 controls brown adipose determination in skeletal muscle satellite cells by targeting Prdm16. (scielo.br)
To directly assess the role of beta cell lipolysis in insulin secretion and whole-body energy homeostasis, inducible beta cell-specific adipose triglyceride lipase (ATGL)-deficient (B-Atgl-KO) mice were studied under normal diet (ND) and high-fat diet (HFD) conditions. (omicsdi.org)
There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)

In living organisms, if the temperature exceeds the normal range (thermo-neutral zone), it disturbs the normal physiological functioning and induces cell injury. (frontiersin.org)

These results suggest that the 3D bioprinted human neural-skeletal muscle constructs can be rapidly integrated with the host neural network, resulting in accelerated muscle function restoration. (nature.com)
More research teams are accelerating the use of other types of adult stem cells, in particular neural stem cells for diseases where beneficial outcome could result from either in-lineage cell replacement or extracellular factors. (biomedcentral.com)

A perivascular origin for mesenchymal stem cells in multiple human organs. (medecinesciences.org)
While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)

Methods: Adult C57BL/6J mice (n=10) were intrathecally injected with a recombinant human NR1 antibody over 14 days and then studied using rs-fMRI at 7 Tesla. (sciencegate.app)
Results: Adult NR1-antibody injected mice showed significantly impaired functional connectivity within the dentate gyrus of the left hippocampus in comparison to controls, resembling impaired hippocampal functional connectivity patterns observed in human patients with NMDAR encephalitis. (sciencegate.app)
Indeed, CXCR4-positive muscle-derived side population (SP) cells home more effectively to dystrophic muscle after intra-arterial delivery in mdx 5cv mice [ 10 ]. (biomedcentral.com)
In the present study, we demonstrate that IRE1α (also known as ERN1) and its downstream target, XBP1, are activated in skeletal muscle of mice upon injury. (elifesciences.org)
Myofiber-specific ablation of IRE1α dampens Notch signaling and canonical NF-κB pathway in skeletal muscle of adult mice. (elifesciences.org)
Deletion of Lkb1 in adult mice results in body weight reduction and lethality. (omicsdi.org)
At 8 weeks of age, these mice were injected with tamoxifen to induce deletion of beta cell-specific Atgl (also known as Pnpla2), and the mice were fed an ND or HFD. (omicsdi.org)
Transplantation of genetically corrected human iPSC-derived progenitors in mice with limb-girdle muscular dystrophy. (medecinesciences.org)

However, diprotin A, a positive modulator of CXCR4-SDF-1 binding, significantly enhanced engraftment and stimulated sustained proliferation of donor cells in vivo . (biomedcentral.com)
Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)

Together, these data suggest that CXCR4 plays an important role in donor cell engraftment. (biomedcentral.com)
Therefore, we sought to translate these results to a clinically relevant canine-to-canine allogeneic transplant model for Duchenne muscular dystrophy (DMD) and determine if CXCR4 is important for donor cell engraftment. (biomedcentral.com)
We show that CXCR4 expressing cells are important for donor muscle cell engraftment, yet FACS sorted CXCR4-positive cells display decreased engraftment efficiency. (biomedcentral.com)
Intramuscular injection of donor muscle-derived cells into chimeric cxmd recipients restored dystrophin expression for at least 24 weeks in the absence of post-transplant immunosuppression, indicating that cell transplantation may be a viable therapeutic option for muscular dystrophy. (biomedcentral.com)
We used the xenotransplant model to show that CXCR4 expression on canine donor muscle cells is also important for cell engraftment, but FACS sorting for CXCR4-positive cells decreased their engraftment efficiency. (biomedcentral.com)

Other key factors would involve electrically stimulating and mechanically stretching the muscles to exercise them, helping them mature properly, and perhaps growing other cells alongside the satellite cells to provide necessary molecular cues. (livescience.com)

Pericytes of human skeletal muscle are myogenic precursors distinct from satellite cells. (medecinesciences.org)

Findings also indicated that acupuncture increased the number of progenitor and satellite cells. (acupunctureofiowa.com)
The findings showed that FT was effective in increasing circulating biomarkers involved in hypertrophy in older adults while adding BFR to FT had a slight increase in these biomarkers but had a tremendous increase in muscle strength. (biomedcentral.com)

These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)

7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)

Intramuscular transplantation of myogenic cells in primates: importance of needle size, cell number, and injection volume. (medecinesciences.org)
Dystrophin expression in muscles of Duchenne muscular dystrophy patients after high-density injections of normal myogenic cells. (medecinesciences.org)

While host nerve integration and formation of NMJs were evident within the implanted skeletal muscle constructs, we observed that the constructs did not support the full restoration of defected muscles at 8-week post-implantation, which is likely due to the delayed integration of host nerve. (nature.com)

Our laboratory aims to establish remedies for refractory muscle diseases, specifically, muscular dystrophy. (kyoto-u.ac.jp)
Taking this advantage, we conduct joint research with pediatricians and neurologists for the purpose of constructing disease models with the use of iPS cells derived from muscular dystrophy patients. (kyoto-u.ac.jp)
Systemic delivery of allogenic muscle stem cells induces long-term muscle repair and clinical efficacy in Duchenne muscular dystrophy dogs. (medecinesciences.org)

Studies previously conducted show that HS negatively affects the skeletal muscle growth and development by changing its effects on myogenic regulatory factors, insulin growth factor-1, and heat-shock proteins. (frontiersin.org)
ATGL in beta cells regulates insulin secretion via the production of signalling MAGs. (omicsdi.org)
Skeletal muscle is an important site of insulin-stimulated glucose disposal and often the site of insulin resistance in obesity. (zen-bio.com)

Transplantation of myogenic stem cells possesses great potential for long-term repair of dystrophic muscle. (biomedcentral.com)
One is cell transplantation and the other is drug development. (kyoto-u.ac.jp)
Regarding the former, we use progenitor cells differentiated from iPS cells as a source for cell transplantation, and evaluate therapeutic effects with disease-model animals. (kyoto-u.ac.jp)
Autologous transplantation of muscle-derived CD133 + stem cells in Duchenne muscle patients. (medecinesciences.org)

Evidence suggests that loss of muscle fiber size and number accelerates after the fifth decade of life [ 10 ]. (hindawi.com)

Cytoskeleton, U-93631 which is a network of interconnected filaments of different types, connects the cell membrane with underlying sub-cellular components. (globaltechbiz.com)

A bioengineered skeletal muscle construct that mimics structural and functional characteristics of native skeletal muscle is a promising therapeutic option to treat extensive muscle defect injuries. (nature.com)

True acupuncture produced superior patient outcomes including improvements in muscle strength, quadriceps angles, thigh circumference, knee range of motion, and Lysholm scores. (acupunctureofiowa.com)
Understanding the effects of strabismus surgery on muscle cell biological reactions and myofiber remodeling may suggest new approaches for improving surgical outcomes. (arvojournals.org)

To obtain a more detailed analysis of the cell mechanics with all U-93631 its major underlying parts, researchers have utilized modeling. (globaltechbiz.com)

Cellular and molecular mechanisms regulating skeletal muscle development. (scielo.br)
The nucleus is the largest organelle among sub-cellular components, demonstrating solid-elastic behavior [4], and it is typically stiffer than the cell itself [5]. (globaltechbiz.com)
This strategy allows the particular elucidation regarding cell-specific prohormone processing along with the finding of mobile or portable cellular signaling peptides.Enterohaemorrhagic Escherichia coli (EHEC) O157:H7 can be a mainly food-borne bacterial virus that's competent at creating life-threatening human being infections and also positions a life threatening obstacle in order to public wellness worldwide. (mrt67307inhibitor.com)

Introduction Cell mechanics has proved to be a widely used label-free biomarker to discern phenotypes, detect pathologies and more importantly, monitor existence or progression of a disease [1C3]. (globaltechbiz.com)
Secondly, they can improve our understanding of the adjustments that occur during disease progression which, in turn, might uncover reasons for corresponding alterations occurring in cell mechanics [10, 11]. (globaltechbiz.com)

however, priority needs to be given to resistance exercise training, and secondarily to adequate protein intake should be part of this dietary regime to mitigate losing muscle mass. (biomed.news)

L1s exert their function as structural elements in the genome, as transcribed RNAs to influence chromatin structure and as retrotransposed elements to shape genomic variation in somatic cells. (sciencegate.app)

Computational approaches to model cell deformation through microfluidic products as complementary of experimental investigations are prominent for multiple reasons. (globaltechbiz.com)

In principle, we could harvest the meat progenitor cells from fresh human cadavers and grow meat from them," Post said. (livescience.com)
Mature human red blood cell (RBC) is perhaps among the simplest cells to model, lacking nucleus and internal cytoskeleton. (globaltechbiz.com)
Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities. (medecinesciences.org)
This strategy has been applied to develop a three-dimensional (3D) human muscle model with endothelium specificity and endomysium for the study of fibrosis 22 . (nature.com)
We also demonstrated the feasibility of using these 3D bioprinted human skeletal muscle constructs to treat critical-sized muscle defect injuries with structural and functional restoration in a rodent model 2 . (nature.com)
Comparison of the Drug-Induced Efficacies between Omidenepag Isopropyl, an EP2 Agonist and PGF2α toward TGF-β2-Modulated Human Trabecular Meshwork (HTM) Cells. (rockland.com)

When activated by injury, satellite cells reenter the cell cycle and proliferate in response to extracellular growth factors ( Relaix & Zammit, 2012 Relaix F, Zammit PS. (scielo.br)

The cell membrane is really a viscous fluid-like matter which includes several lipids, cholesterol, and inserted proteins. (globaltechbiz.com)

Anatomy66

Organisms18

Diseases12

Chemicals and Drugs76

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes56

Disciplines and Occupations1

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture1

Information Science5

Health Care2

Regeneration29

Embryonic7

Differentiation13

Fibers6

Atrophy5

Myoblasts6

Isoforms4

Vitro5

Primary skeletal1

Myogenesis1

Stem Cell6

Quiescent3

Tissue16

Murine4

Precursor cells1

Myofibers6

Regenerate2

Sarcopenia7

Pax32

Metabolism3

Tissues2

Adipose3

Physiological1

Neural2

Mesenchymal stem2

Mice8

Vivo2

Donor5

Cues1

Precursors1

Findings2

Basal lamina1

Limb muscle1

Myogenic cells2

NMJs1

Muscular dystrophy3

Insulin3

Transplantation4

Accelerates1

Network of interconnected1

Therapeutic option1

Outcomes2

Researchers1

Cellular3

Progression2

Mitigate losing1

Somatic cells1

Approaches1

Human6

Proliferate1

Proteins1