Satellite cell myofibers pax7 transcription factor. Medical search. Frequent questions

These cells are located beneath the basal lamina of myofiber in a dormant state. (elifesciences.org)
2011). Satellite cells are located in a distinct segment between a layer of connective tissue (basal lamina) and muscle fibers (myofibers). (sciencetrend.ca)
1 Adult Stem Cells 1.1 Satellite Cells Studies in the last decade have clearly proven that the regenerative ability of adult skeletal muscle is due to the satellite cell a quiescent stem cell population of muscle precursors located between the basal lamina and sarcolemma of each myofiber. (biotech-angels.com)
1 2 Residing outside the sarcolemma but within the basal lamina of individual myofibers is a population of cells that are responsible for muscle repair and regeneration, the satellite cells. (arvojournals.org)
For example, muscle stem cells (MuSCs), located beneath the basal lamina, exist in the quiescent state but can transition to an activated, proliferative state upon injury. (biomed.news)

Furthermore, we have successfully predicted and validated that the transcription factor myogenic differentiation (MYOD) binds to the MEG3 core promoter though utilizing chromatin immunoprecipitation (CHIP) and luciferase reporter assays. (bvsalud.org)
The results indicated that MYOD acts as a transcription factor of MEG3 to promote MEG3 transcription. (bvsalud.org)
It is concluded that MYOD acts as a transcription factor to induce MEG3 expression. (bvsalud.org)
The bHLH transcription factor MyoD is a master regulator of myogenic differentiation, and its sustained expression in fibroblasts suffices to differentiate them into muscle cells. (bvsalud.org)
MyoD expression oscillates in activated muscle stem cells of developing, postnatal and adult muscle under various conditions: when the stem cells are dispersed in culture, when they remain associated with single muscle fibers, or when they reside in muscle biopsies. (bvsalud.org)
Unstable MyoD oscillations and long periods of sustained MyoD expression are observed when stem cells undergo myogenic differentiation. (bvsalud.org)
The oscillatory expression of MyoD is driven by the oscillatory expression of the bHLH transcription factor Hes1 that periodically represses MyoD. (bvsalud.org)
Thus, oscillations of MyoD and Hes1 control the balance between the proliferation and differentiation of muscle stem cells. (bvsalud.org)
Here, we describe time-lapse imaging methods using luciferase reporters, which can monitor dynamic MyoD gene expression in myogenic cells. (bvsalud.org)
Here, we examined the time-dependent expression patterns of multiple biomarkers associated with satellite cell fate, including the transcription factor paired box 7 (Pax7), myoblast determination protein (MyoD), myogenin, and insulin-like growth factor (IGF-1), using immunohistochemistry, western blotting, and quantitative real-time PCR in contused skeletal muscle. (bvsalud.org)
Morphometrically, the data obtained from the numbers of Pax7 + , MyoD + , and myogenin + cells were highly correlated with the wound age. (bvsalud.org)
Pax7, MyoD, myogenin, and IGF-1 expression patterns were upregulated after injury at both the mRNA and protein levels. (bvsalud.org)
Pax7, MyoD, and myogenin protein expression levels confirmed the results of the morphometrical analysis. (bvsalud.org)
Myogenic regulatory factors (MRFs): MyoD, Myf5, Myf6 and Myogenin. (wikipedia.org)
PAX3 mediates the transcription of c-Met and is responsible for the activation of MyoD expression-one of the functions of MyoD is to promote the regenerative ability of satellite cells (described below). (wikipedia.org)
Associated Genetic Factors: PAX3, c-Met, Mox2, MSX1, Six, Myf5, and MyoD Mox2 (also referred to as MEOX-2) plays an important role in the induction of mesoderm and regional specification. (wikipedia.org)
Persistent expression of C/EBPβ upregulates Pax7, inhibits MyoD, and blocks myogenic differentiation. (biomedcentral.com)
When overexpressed in the murine myoblast cell line C2C12 or in primary myoblasts, C/EBPβ reduced MyoD and other myogenic protein levels during differentiation, in addition to reducing myoblast fusion. (biomedcentral.com)
Their fate is regulated by the expression of various transcription factors, including paired box 7 (Pax7) and myogenic differentiation (MyoD) [ 6 ]. (biomedcentral.com)
Upon activation satellite cells rapidly initiate MyoD expression which leads to the activation of myogenin and terminally differentiated structural muscle mass genes such as myosin heavy chain (MHC) [1] [3]. (healthyguide.info)
Interestingly recent data indicated that although MyoD is not indicated in quiescent satellite cells in the adult it is transiently indicated in satellite cell progenitors in the embryo suggesting that satellite cells are derived from committed embryonic precursors of myogenic lineage [4] [5]. (healthyguide.info)
All superior and inferior rectus muscles from both globes were examined for BrdU incorporation, MyoD expression, neonatal and developmental myosin heavy chain (MyHC) isoform expression, and myofiber cross-sectional area alterations. (arvojournals.org)
In the resected muscle and in the passively stretched antagonist muscle, there was a dramatic increase in the number of myofibers positive for neonatal MyHC and in the number of BrdU- and MyoD-positive satellite cells. (arvojournals.org)

If placed in cell culture, most myoblasts will proliferate if enough fibroblast growth factor (FGF) or another growth factor is present in the medium surrounding the cells. (wikipedia.org)
When the growth factor runs out, the myoblasts cease division and undergo terminal differentiation into myotubes. (wikipedia.org)
Loss of C/EBPβ in SCs results in precocious differentiation at the expense of self-renewal in both primary myoblasts and myofiber cultures. (biomedcentral.com)
After several rounds of proliferation, most SCs differentiate into myoblasts, which fuse to form new myofibers [ 5 ]. (biomedcentral.com)
3 20 21 Major factors underlining this poor outcome included low ability of myoblasts to migrate beyond the injection site[22 23 and poor survival of injected cells. (biotech-angels.com)
26-29] As an alternative to poorly-engrafting myoblasts much recent interest has developed around the idea of therapy with stem cells. (biotech-angels.com)
34] Upon damage satellite television cells become activated providing rise to proliferating myoblasts which in turn fuse to existing muscle tissue fibers or even to additional myoblasts to URMC-099 create new myofibers to correct muscle tissue harm. (biotech-angels.com)
Using synchronized cultures of C2C12 myoblasts to model these divergent programs, we show that p8 (also known as Nupr1 ), a G1-induced gene, negatively regulates the cell cycle and promotes myogenic differentiation. (biologists.com)

Associated Genetic Factors: PAX3 and c-Met Mutations in PAX3 can cause a failure in c-Met expression. (wikipedia.org)
PAX3 is needed for the transcription of c-Met. (wikipedia.org)
Finally we found that in an mouse model of fracture healing where muscle mass progenitor cells were lineage-traced Nkx3.2 and Sox9 are significantly upregulated while Pax3 is significantly downregulated in the muscle mass progenitor cells that give rise to chondrocytes during fracture restoration. (healthyguide.info)
Therefore our and analyses suggest that the balance of Pax3 Nkx3.2 and Sox9 may act as a molecular Talniflumate switch during the chondrogenic differentiation of muscle mass progenitor cells which may be important for fracture healing. (healthyguide.info)
Collectively our data suggest that the balance of Nkx3.2 Sox9 and Pax3 can act as a molecular switch during the chondrogenic differentiation of satellite cells which may play an important part in the healing process experiments were normalized to GAPDH. (healthyguide.info)
30] The additional approach used a transgenic reporter mouse for Pax3 a paralog of Pax7 which allowed for the immediate isolation of Pax3+ (GFP+) muscle satellite television cells by movement cytometry. (biotech-angels.com)
We demonstrate that mdPCR can give exact molecule counts per cell for mRNA isoforms encoding the myogenic transcription factor Pax3. (biomed.news)

Myofiber-specific ablation of IRE1α or XBP1 in mice diminishes skeletal muscle regeneration that is accompanied with reduced number of satellite cells. (elifesciences.org)
Finally, targeted ablation of IRE1α also reduces Notch signaling, abundance of satellite cells, and skeletal muscle regeneration in the mdx mice, a model of Duchenne muscular dystrophy. (elifesciences.org)
Collectively, our experiments suggest that the IRE1α-mediated signaling promotes muscle regeneration through augmenting the proliferation of satellite cells in a cell non-autonomous manner. (elifesciences.org)
The findings shed new light on mechanisms of regeneration of healthy myofibers after severe tissue injury and suggest interplay between these fibers and muscle satellite cells though mechanisms remain to be elucidated. (elifesciences.org)
Using genetic tools to conditionally abrogate C/EBPβ expression in Pax7 + cells, we examined the role of C/EBPβ in self-renewal of satellite cells during muscle regeneration. (biomedcentral.com)
After a single muscle injury, C/EBPβ-deficient satellite cells fail to self-renew resulting in a reduction of satellite cells available for future rounds of regeneration. (biomedcentral.com)
These findings demonstrate that C/EBPβ is a novel regulator of satellite cell self-renewal during muscle regeneration acting at least in part through Notch2. (biomedcentral.com)
Satellite cells (SCs), which are precursors of skeletal muscles, such as the TA muscle, play a key role in muscle homeostasis and regeneration. (biomedcentral.com)
Muscle satellite cells make up a stem cell populace that is capable of differentiating into myocytes and contributing to muscle mass regeneration upon injury. (healthyguide.info)
In recent research, scientists unlocked ways to boost muscle regeneration in the experimental animal by activating the precursors of muscle cells. (sciencetrend.ca)
In recent research, scientists managed to activate the precursors of muscle cells, which contributes to aiding muscular regeneration and rebuilding damaged tissue. (sciencetrend.ca)
Salk Institute researchers are finding methods to speed up methods to accelerate the regeneration of muscle tissue, utilizing a combination of molecular compounds that are generally utilized in the stem-cell analysis. (sciencetrend.ca)
2021), the investigators confirmed that utilizing these compounds increased the regeneration of muscle cells in mice by activating the precursors of muscle cells, known as myogenic progenitors. (sciencetrend.ca)
Within the myofiber-specific model, they discovered that adding the Yamanaka factors accelerated muscle regeneration in mice by decreasing the levels of a protein referred to as Wnt4 in the area of interest, which activated the satellite cells. (sciencetrend.ca)
In contrast, Yamanaka factors didn't activate satellite cells within the satellite-cell-specific model and didn't enhance muscle regeneration, suggesting that Wnt4 performs an essential role in muscle regeneration. (sciencetrend.ca)
An absolute requirement for Pax7-positive satellite cells in acute injury-induced skeletal muscle regeneration. (sciencetrend.ca)
In vivo partial reprogramming of myofibers promotes muscle regeneration by remodeling the stem cell niche. (sciencetrend.ca)
Loss of either Dll4 or Mib from the myofiber reduces SC numbers and impairs muscle regeneration. (sciety.org)
6 These manipulations result in satellite cell activation and proliferation, which in turn are responsible for muscle repair and regeneration. (arvojournals.org)

The investigators are also studying different methods to rejuvenate cells, including utilizing mRNA and genetic engineering. (sciencetrend.ca)
Quantifying the exact expression levels of the mRNA isoforms encoding these myogenic transcription factors is therefore key to understanding how MuSCs switch between cell states. (biomed.news)
We isolated both damaged and intact myofibers from a mouse model of muscular dystrophy using laser-capture microdissection and found that the levels of myoferlin mRNA and protein were increased in damaged myofibers. (biologists.com)

Ex vivo cultures of myofiber explants demonstrate that ablation of IRE1α reduces the proliferative capacity of myofiber-associated satellite cells. (elifesciences.org)
30-32] The satellite cell was first described by Mauro in 1961 using electron microscopy[33] and later by Bishoff in 1986 utilizing phase-contrast microscopy on solitary myofiber explants. (biotech-angels.com)

Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population. (biomedcentral.com)
These cells have the ability to self-renew and to differentiate into specialized cell types and can be primarily classified as adult and pluripotent stem cells which differ significantly in regard to their differentiation potential and expansion capability. (biotech-angels.com)
Adult stem cells are tissue specific and have limited capacity to be expanded while pluripotent stem cells have the ability to differentiate into any cell type of the body while possessing unlimited self-renewal. (biotech-angels.com)

Proliferating, BrdU-positive cells in muscle sections were detected with immunohistochemistry indicating different cell types and decreasing proliferation with age. (nature.com)
Cell culture experiments indicated that Gln could promote cell proliferation in a dose dependent manner, but expression of myogenesis regulatory genes was not altered. (nature.com)
Overall, Gln supplementation stimulated cell proliferation in muscle tissue and in vitro in myogenic cell culture, whereas muscle growth regulatory genes were barely altered. (nature.com)
A single, early local administration of high-dose bFGF prevented atrophic changes in the thyroarytenoid muscles by activating satellite cell proliferation and reforming neuromuscular junctions. (biomedcentral.com)
Objective: The aim of this study was to reveal the role and regulatory mechanism of miR-188-5p in the proliferation and differentiation of goat muscle satellite cells. (koreascience.kr)
Overexpression and interference of miR-188-5p showed that miR-188-5p inhibited the proliferation and promoted the differentiation of goat muscle satellite cells. (koreascience.kr)
Further functional studies revealed that CAMK2B promoted the proliferation and inhibited the differentiation of goat muscle satellite cells, whereas si-CAMK2B restored the function of miR-188-5p inhibitor. (koreascience.kr)
Conclusion: These results suggest that miR-188-5p inhibits the proliferation and promotes the differentiation of goat muscle satellite cells by targeting CAMK2B. (koreascience.kr)
31] Down the road Sacco and colleagues proven that intra-muscular transplantation of an individual luciferase-expressing muscle stem cell isolated from Myf5 reporter mice led to intensive proliferation and contribution to muscle materials. (biotech-angels.com)

Furthermore these authors demonstrated that Pax7(+)luciferase(+) mononuclear cells could possibly be readily re-isolated offering proof for the self-renewal of the cell human population. (biotech-angels.com)

One such alternative cell fate is the adipogenic fate as Pax7(+) satellite cells isolated from solitary myofibers used adipogenic fate in addition ARHGAP1 to muscle mass fate significance of these factors we used a mouse fracture healing model inside a genetically altered reporter mouse where muscle mass progenitors were lineage-traced. (healthyguide.info)

Satellite cells from your trunk and the limb are derived from an embryonic populace of progenitor cells in the somites transient mesodermal constructions that develop on either part of the neural tube [1]. (healthyguide.info)
Adult regenerative myogenesis is dependent on progenitor cells. (biologists.com)

This interferes with the maintenance of activated muscle stem cells and impairs muscle growth and repair. (bvsalud.org)

Previous studies have shown that lncRNA-MEG3 promotes the differentiation of porcine skeletal muscle satellite cells (PSCs), but the regulatory mechanism of MEG3 interaction with target protein has not been well studied. (bvsalud.org)
Methods: Goat skeletal muscle satellite cells isolated in the pre-laboratory were used as the test material. (koreascience.kr)
In addition, miR-188-5p was transfected into goat skeletal muscle satellite cells by constructing mimics and inhibitors of miR-188-5p, respectively. (koreascience.kr)

We find that loss of C/EBPβ leads to precocious differentiation at the expense of self-renewal in primary myoblast and myofiber cultures. (biomedcentral.com)

The use of a muscle creatine kinase germline knockout of the gene encoding Ire1/Ern1 shifts the focus from traditional studies of tissue repair toward the regenerating myofibers. (elifesciences.org)
Myf5 is considered to be the earliest expressed regulatory factor gene in myogenesis. (wikipedia.org)
The abundance of Dll4 captured by the QSC correlates with levels of the SC identity gene, Pax7. (sciety.org)

The addition of BrdU-positive myonuclei increased from 1 per 1000 myofibers in cross sections of control muscles to 2 to 3 per 100 myofibers in the resected muscles. (arvojournals.org)
Single myofiber reconstructions showed that multiple BrdU-positive myonuclei were added to individual myofibers. (arvojournals.org)
Addition of new myonuclei occurred in random locations along the myofiber length of single fibers. (arvojournals.org)
There was no correlation between myofibers with BrdU-positive myonuclei and neonatal MyHC isoform expression. (arvojournals.org)
Both active and passive stretch of the rectus muscles produced by strabismus surgery dramatically upregulated the processes of satellite cell activation, integration of new myonuclei into existing myofibers, and concomitant upregulation of immature myosin heavy chain isoforms. (arvojournals.org)
It is considered postmitotic, in that the myonuclei residing within these myofibers do not divide in adult muscle. (arvojournals.org)

There are a number of factors that initiate myofiber remodeling in adult skeletal muscles. (arvojournals.org)

The transcription factor CCAAT/enhancer binding protein beta (C/EBPβ) is expressed in Pax7 + satellite cells of healthy muscle and is downregulated during myoblast differentiation. (biomedcentral.com)

Research on regenerative medicine using basic fibroblast growth factor (bFGF) has recently advanced in the field of laryngology. (biomedcentral.com)
The basic fibroblast growth factor (bFGF) is a member of the fibroblast growth factor family and is a key protein in regenerative medicine. (biomedcentral.com)

Upon injury or when challenged with a variety of mechanical or biochemical stimuli satellite cells re-enter the cell cycle and give rise to Talniflumate differentiated myocytes which form new muscle mass materials or fuse with existing materials and contribute to muscle mass growth and restoration [1]. (healthyguide.info)

30 31 One group took the approach of transplanting sole muscle fibers which demonstrated that every myofiber including 7 or fewer satellite television cells could create over 100 new myofibers in engrafted muscles. (biotech-angels.com)
3 When a muscle is injured, this specialized cell population becomes activated, divides, and either repairs damaged fibers or forms new myofibers. (arvojournals.org)

The quiescent muscle stem cell (QSC) pool is heterogeneous and generally characterized by the presence and levels of intrinsic myogenic transcription factors. (sciety.org)

Muscle samples were collected and myogenic cells were isolated and cultivated. (nature.com)

The first stage, involves cell cycle exit and the commencement of expression of certain genes. (wikipedia.org)
Serum response factor (SRF) plays a central role during myogenesis, being required for the expression of striated alpha-actin genes. (wikipedia.org)
First, the expression of miR-188-5p in goat muscle tissues at different developmental stages was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). (koreascience.kr)
One significant issue with the manuscript is the "discovery" of an SC continuum related to the relative levels of Pax7 expression. (sciety.org)
In the original manuscript we acknowledged that SCs exist on a continuum-a gradual transition from one state to another, based on scRNA-seq studies and the present data (Dll4, Pax7 and Ddx6 expression). (sciety.org)
31] Cells isolated from mature skeletal muscles displayed homogenous expression of Pax7 and contributed to both dietary fiber repair also to the muscle satellite television cell URMC-099 compartment subsequent their transplantation into dystrophic mice. (biotech-angels.com)
The control of MuSC state depends on the expression levels of myogenic transcription factors. (biomed.news)

The compromised tissue repair program is attributable to the gradual depletion of stem cells and to altered regulatory signals. (biomed.news)
Adult stem cells play key roles in homeostasis and tissue repair. (biomed.news)

Immunohistochemistry indicated that bFGF significantly increased the number of satellite cells in the thyroarytenoid muscle up to day 14 and that of neuromuscular junctions on days 28 and 56. (biomedcentral.com)

In the embryo, zygotic transcription of Eph is confined to the nervous system. (sdbonline.org)

Results: It was highly expressed in adult goat latissimus dorsi and leg muscles, goat fetal skeletal muscle, and at the differentiation stage of muscle satellite cells. (koreascience.kr)
Irradiation treatment, which eliminates satellite cells from these muscles, prevents the compensatory hypertrophy in these types of experiments. (arvojournals.org)

Each stage has various associated genetic factors lack of which will result in muscular defects. (wikipedia.org)

However satellite cells have since been shown to have the ability to adopt option cell fates. (healthyguide.info)
conclude that Dll4 and Mib present on myofibers maintain a continuum of SC fates providing SCs capable of regenerating muscle and repopulatin the SC niche. (sciety.org)

Therefore, we complemented our investigations into the cellular development of the skeletal muscle of neonatal piglets with in vitro studies using a primary porcine myogenic cell culture model with Gln supplementation. (nature.com)

In lab analysis, they're used to transform specialized cells, like skin cells, into extra stem-cell-like cells that are pluripotent, suggesting they have the ability to become many various kinds of cells. (sciencetrend.ca)

35-39] Meanwhile a little subset of satellite television cells will not undergo differentiation but wthhold the ability to go back to a quiescent state and therefore preserve the satellite television cell pool. (biotech-angels.com)

The identification of heterogenous distribution of factors across the myofiber is an important contribution for dissecting how muscle stem cell diversity in a tissue is achieved. (sciety.org)

For example, these manuscripts establish that elevated Pax7 levels drive quiescence and low Pax7 levels correlate with differentiation. (sciety.org)
Jyotsna Dhawan ABSTRACT Adult stem cells persist in mammalian tissues by entering a state of reversible quiescence, referred to as G 0 , which is associated with low levels of transcription. (biologists.com)

Our data shows that a post-translational mechanism controls spatial heterogeneity of Notch ligands in a multinucleated niche cell to maintain a continuum of diverse states within the SC pool during tissue homeostasis. (sciety.org)
There is certainly proof for heterogeneity inside the satellite television cell compartment having a subset of satellite television cells having higher potential to engraft the satellite television cell area. (biotech-angels.com)

In this research, the team used two completely different mouse models to pinpoint the muscle stem-cell-specific or niche-specific modifications following the addition of Yamanaka factors. (sciencetrend.ca)

CCAAT/enhancer binding proteins (C/EBPs) are a family of transcription factors that regulate cellular growth and differentiation, metabolism, and inflammation [ 18 ]. (biomedcentral.com)

Semaphorins are a wide family of extracellular regulatory signals with a multifaceted role in cell-cell communication. (biomed.news)

Associated Genetic Factors: c-Met/HGF and LBX1 Mutations in these genetic factors causes a lack of migration. (wikipedia.org)
These consequences further reveal the complexity of myogenesis and the importance of each genetic factor in proper muscle development. (wikipedia.org)

J Cell Sci (2022) 135 (15): jcs259789. (biologists.com)

Originally discovered as axon guidance factors, they have been implicated in cancer progression, embryonal organogenesis, skeletal muscle innervation, and other physiological and developmental functions in different tissues. (biomed.news)

7 8 9 In fact, passive stretching alone can activate limb muscle satellite cells. (arvojournals.org)
10 Even more compelling is a recent study that subjected individual quiescent satellite cells isolated from adult limb muscle to stretching in vitro, and showed that direct stretching of the satellite cells themselves results in activation and bromodeoxyuridine (BrdU) incorporation. (arvojournals.org)

Anatomy26

Organisms14

Diseases7

Chemicals and Drugs116

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes61

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science5

Health Care1

Basal lamina5

MyoD23

Myoblasts8

PAX37

Regeneration19

MRNA3

Explants2

Differentiate3

Proliferation9

Luciferase1

Solitary1

Progenitor cells2

Impairs muscle1

Skeletal muscle satellite cells3

Cultures1

Gene3

Myonuclei6

Initiate1

Myoblast differentiation1

Fibroblast growth2

Fuse1

Fibers2

Quiescent muscle1

Myogenic cells1

Expression7

Tissue repair2

Immunohistochemistry1

Embryo1

Muscles2

Muscular1

Fates2

Porcine1

Pluripotent1

Undergo1

Contribution1

Quiescence2

Heterogeneity2

Muscle stem cell1

Regulate1

Regulatory1

Genetic2

20221

Developmental1

Limb2