The goal of this research project was to determine if eDNA isolated from water samples could be used to estimate the population of the northern crested newt (Triturus cristatus) and the common newt (Triturus vulgaris), results that will be summarized in a second report. (nina.no)
High temperature can induce female-to-male sex 3 College of Fisheries, Huazhong Agricultural University, reversal in Triturus cristatus (Wallace et al. (docslib.org)
Great crested newt ( Triturus cristatus ) - listed in Annexes II and IV of the EC Habitats Directive and Appendix II of the Bern Convention, also protected under Schedule 2 of The Conservation (Natural Habitats, &c. (forestresearch.gov.uk)
Newts5
A During 2010-2016, we collected samples of free-living mphibians provide an iconic example of disease- populations of newts and salamanders from 48 sites in driven global loss in biodiversity. (cdc.gov)
Although recent studies 2 have indicated the Great crested newts DNA can be found in the water at any time of the year, Natural England will only accept eDNA survey results from samples collected between 15 April and 30 June. (greatcrestednewtedna.co.uk)
Reciprocal non-monophyly has been established through molecular studies for the European mountain newts (Calotriton, Euproctus) and the modern European newts (Ichthyosaura, Lissotriton, Neurergus, Ommatotriton, Triturus) since Calotriton was identified as the sister lineage of Triturus. (hbz-nrw.de)
Previous molecular trees based on seemingly large nuclear data sets, but analysed together with mitochondrial data, did not reveal monophyly of modern European newts since taxon sampling and nuclear gene coverage was too poor to obtain conclusive results. (hbz-nrw.de)
EDNA8
As part of a research project financed by the Norwegian Environment Agency (Miljødirektoratet), NINA has collected water samples from 34 different ponds in Akershus and Buskerud County for analysis of environmental DNA (eDNA). (nina.no)
eDNA is DNA that is collected from a range of environmental samples including soil and water rather than being directly sampled from a plant or animal. (greatcrestednewtedna.co.uk)
eDNA analysis can provide a presence/absence result after just a single site sampling visit. (greatcrestednewtedna.co.uk)
eDNA analysis is a relatively new methodology where the presence of a species is detected by analysis of the DNA contained within an environmental sample (e.g. water or soil). (greatcrestednewtedna.co.uk)
The steps in eDNA methods include water sample collection from the field, DNA extraction, design and validation of species-specific primer, sequencing, and taxonomic assignment. (crimsonpublishers.com)
The eDNA method is more cost and time effective than these traditional sampling techniques. (crimsonpublishers.com)
The eDNA signal and detection probability of the introduced species were strongest near the keepnets, resulting in the highest community variance of different sampling events at this position. (worktribe.com)
Published probe-based qPCR assays for species detection in eDNA samples (updated May 2019). (wsu.edu)
Vulgaris2
Formålet har vært å undersøke om DNA isolert fra vannprøver kan brukes til å anslå tetthet av stor (Triturus cris-tatus) og liten salamander (Triturus vulgaris) og å teste ut om metodikken kan brukes til fremtidig overvåkning av soppene Batrachochytrium dendrobatidis (Bd) og Batrachochytrium salamandrivorans (Bsal), som parasitterer amfibier. (nina.no)
A controlled study in 1977-1984 in two lakes in south western Sweden (Aronsson & Stenson 1995) found that fish elimination using rotenone resulted in a rapid increase in the smooth newt Triturus vulgaris population. (conservationevidencejournal.com)
Species4
Sometime there is an issue of false positive or negative detection of species that can be reduced by following the protocol from sample collection to analysis and standardization of method for species monitoring. (crimsonpublishers.com)
Meetnetten.be - Occupancy estimates of priority vascular plants species in Flanders, Belgium is a sampling event dataset published by the Research Institute of Nature and Forest (INBO). (vlaanderen.be)
Meetnetten.be - Population counts of priority vascular plants species in Flanders, Belgium is a sampling event dataset published by the Research Institute of Nature and Forest (INBO). (vlaanderen.be)
The introduced species were no longer detected at any sampling positions on 48 hrs after removal from the ponds. (worktribe.com)
Alpestris1
Among the fauna inhabiting the reservoirs of the Upper Nalon, we find many amphibians -most of them protected- like the common toad (Bufo bufo), the midwife toad (Alytes obstetricans), the green frog (Rana perezi), the Alpine newt (Triturus alpestris), the Iberian newt (Triturus boscai), the fire salamander (Salamandra salamandra) and the Gold-striped salamander (Chioglossa lusitanica). (amber.international)
Tissue samples4
The LVL genetic sampling tool was designed in collaboration with European fisheries scientists and fishing industry organizations and represents a significant advance in the collection and storing of fish tissue samples for genetic analyses. (lvl-technologies.com)
This removes the need for sub-sampling of tissue samples thus representing a significant time saving and increasing sample throughput. (lvl-technologies.com)
It also removes any potential of sources contamination by eliminating direct handling of tissue samples. (lvl-technologies.com)
All assays should be re-validated with tissue samples when using them under new conditions. (wsu.edu)
Biodiversity1
Single samples can be used for the estimation of biodiversity of a particular area. (crimsonpublishers.com)
Occurrence1
Here it is published as a standardized Darwin Core Archive and includes for each sampling event an eventID, date, location and sampling protocol (in the event core)and for each occurrence an occurrenceID, the occupied m² recorded, status (present/absent) and scientific name (in the occurrence extension). (vlaanderen.be)
Population3
NHANES III is a stratified multistage probability sample of the civilian non- institutionalized population of the U.S. (cdc.gov)
Drift fences are frequently used to sample amphibians for population studies. (researchgate.net)
It is now possible to genotype large numbers of samples at large numbers of genetic markers, in order to identify population or stock structure, for relatively low costs. (lvl-technologies.com)
Enables3
We would like to reassure you that our extensive experience which often involves working with challenging DNA samples enables us to deliver a "Forensic Lab Quality" service for you. (greatcrestednewtedna.co.uk)
As levels of sampling increase and international genetic sampling programs are developed, there is a need for a specific genetic sample collection tool in order to ensure that a standardized collection and storage method is implemented, which ensures high quality samples and enables downstream automation of sample processing. (lvl-technologies.com)
When used in conjunction with the LVL Manual 1-Channel Capper/Decapper/Picker, it enables the user to select a sampling tool from a specific tube, to collect a genetic sample from the fish and return the tool, with the sample in it, to the collection tube without ever touching the tube or sample directly, thus reducing the possibility of cross contamination. (lvl-technologies.com)
Common1
There was not a common sampling method for all the specimens. (gbif.org)
Extraction1
During DNA extraction the semi-automated SAFE ® 8-channel capper/decapper can be used to remove eight sample tool caps, with samples, from the sample tubes and transfer them to the LVL low profile deep-well plates (product code: 225.DW.1.2.PP) for the digestion stage of DNA extraction. (lvl-technologies.com)
Collection1
The unique sampling tool is designed specifically for use on fish and the pointed tip and rear facing cutting edge enable collection of a c.30mg tissue sample from beneath the skin of the fish being sampled, thus avoiding surface contamination. (lvl-technologies.com)
Years1
The phylogenetic relationships among members of the family Salamandridae have been repeatedly investigated over the last 90 years, with changing character and taxon sampling. (hbz-nrw.de)
Protocol1
Water samples collected are sent to Cellmark's laboratories for testing using the protocol recommended by Natural England . (greatcrestednewtedna.co.uk)
Skin1
Technische Universität Braunschweig, skin ( 7 , 8 ) of live animals and collecting skin samples from Braunschweig, Germany (E.K. Bales, M.C. Bletz, J. Sabino-Pinto, dead animals. (cdc.gov)
Animals1
The olfactory system of Triturus pyrrhogaster , changes morphologically and physiologically when the animals are kept either in a terrestrial or an aquatic environment [ 15 , 16 ]. (biomedcentral.com)
Tool1
The novel system is based on LVL's range of SAFE ® biobanking consumables and pairs a standard SBS format 96-tube barcoded rack and internal threaded 2D barcoded tubes with a genetic sampling tool incorporated into the screwcap of the tubes. (lvl-technologies.com)
Data1
We would appreciate it if you follow the INBO norms for data use (https://www.inbo.be/en/norms-data-use) when using the data. (vlaanderen.be)
Status1
Ensure correct functioning and status of the Triturus ELISA with highly precise and stable hardware and software. (cytomed.ae)
Biological1
A completely open and fully automated ELISA analyzer for testing and processing batches of samples for infectious diseases, autoimmunity, and biological drug monitoring. (cytomed.ae)
Open1
To obtain samples, monkeys are often restrained in open restraint chairs (ORC) with the "pole and collar" technique. (awionline.org)
Single2
Perform up to 8 different tests per batch on a single sample or group of samples (e.g. (cytomed.ae)
The HELIOS is a revolutionary platform that both processes and analyzes patient samples in a single run. (aesku.com)
Analysis1
On the management side of things, I also handle the receipt, analysis, and storage of pharmacokinetic samples. (sheffield.ac.uk)