Sample of cells. Medical search. Frequent questions

There were 15 primary lung cancer cases with sufficient tissue for analysis: 8 were men, 7 were stage I, 7 had adenocarcinoma, 8 had squamous cell carcinoma, 8 were former smokers, 7 were current smokers, 9 were Caucasian, and 6 were African-American. (aacrjournals.org)

Briefly, extracellular RNA (most commonly released upon cell lysis) is captured along with each cell in its reaction chamber, contributing counts to genes that are not otherwise expressed in that cell (see Advanced Section 7.2 ). (bioconductor.org)
Two sets of genes that make normal hemoglobin (hemoglobin A). These people have normal red blood cells unless they have some other blood disease. (healthwise.net)
One set of genes that makes normal hemoglobin (hemoglobin A) and one set that makes hemoglobin S. These people carry the sickle cell trait (and are called " carriers "), but they do not have sickle cell disease. (healthwise.net)
Two sets of genes that make hemoglobin S. These people have sickle cell disease. (healthwise.net)
In sickle cell disease, a person has two abnormal hemoglobin S genes. (medlineplus.gov)
A person with sickle cell trait has only one of these abnormal genes and no symptoms, or only mild ones. (medlineplus.gov)
A multiplicity of genes was shown to be differentially expressed in immediately processed CD4+ T-cells from atopic versus healthy subjects . (bvsalud.org)
These differences disappeared when comparing delayed processed cells due to a drastic change in expression levels of atopy-related genes in delayed processed CD4+ T-cells from atopic donors . (bvsalud.org)
Its scope is to categorize the 37 trillion cells of the human body to determine which genes each cell expresses by sampling cells from all parts of the body. (wikipedia.org)
Sickle-cell anaemia is a common genetic condition due to a haemoglobin disorder - inheritance of mutant haemoglobin genes from both parents. (who.int)
About 5% of the world's population carries genes responsible for haemoglobinopathies and each year about 300 000 infants are born with major haemoglobin disorders - in more than 200 000 cases sickle-cell anaemia in Africa. (who.int)
Although a single abnormal gene may protect against malaria, inheritance of two abnormal genes leads to sickle-cell anaemia and confers no such protection, and malaria is a major cause of ill-health and death in children with sickle-cell anaemia. (who.int)

These cells come standard with HPLC fittings which transform your polarimeter into a chiral detector when connected to a chiral column after HPLC (Picture D). (rudolphresearch.com)
The best way to check for sickle cell trait or sickle cell disease is to look at the blood using a method called high-performance liquid chromatography (HPLC). (healthwise.net)
Heterozygous individuals ried out using high performance liquid have sickle cell trait, a generally benign, as- chromatography (HPLC). (who.int)

We also describe integrated modules for immunofluorescent staining of sectioned tissue, bulk tissue genomic analysis and fluorescence- or magnetic-activated cell sorting of digested tissue for subsequent culture or transcriptomic analysis by RNA sequencing. (nature.com)
Cytopainter (ab187964) is designed to uniformly label live cells in green fluorescence. (abcam.com)
Abcam's CytoPainter Live Cell Labeling Kits are a set of tools used to label cells for fluorescence microscopic investigations and flow cytometric investigations of cellular functions. (abcam.com)
Image of CPA cells stained with CytoPainter Live Cell Labeling Kit - Green Fluorescence (ab187964) in a black wall/clear bottom 96-well plate. (abcam.com)
In this study, we developed a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by combining (i) nontoxic fluorescence staining of polyphosphate granules in viable microbial cells and (ii) fluorescence-activated cell sorting (FACS) for the rapid detection and collection of target cells. (frontiersin.org)
In this study, we propose a strategy for the isolation of polyphosphate-accumulating organisms (PAOs) by coupling (i) specific staining of an intracellular accumulate without compromising cell viability, and (ii) rapid detection and sorting of target cells by using fluorescence-activated cell sorting (FACS), for which wastewater sludge samples were utilized. (frontiersin.org)
The fluorochrome-labeled cells are analyzed by using a flow cytometer, which categorizes individual cells according to size, granularity, fluorochrome, and intensity of fluorescence. (cdc.gov)
The authenticity of the histamine like fluorescence was checked by treating some of the samples with diamine-oxidase before fluorometric analysis. (cdc.gov)

Leukocytes prepared from venous blood samples drawn from two volunteers were incubated alone or with opsonized zymogen for 1 hour at 37 degrees-C to stimulate the neutrophils. (cdc.gov)

Fig. 3: Variation in immune cell abundance and cell viability across different brain (tumor) tissue types. (nature.com)
It has been discovered that a minimal change in the resonance of the acoustic field (i.e., introducing a sample with a slightly different density) can lead to a collapse of the trapping force within the cell, limiting the viability of this method to acoustically closely matched samples and solvents. (tuwien.at)

The assay is ideal if working with limited sample amounts, hard to culture cell lines, or small tissue biopsies. (activemotif.com)

Background: Circulating tumour cells (CTCs) are cells that have detached from a primary tumour, circulate in the peripheral blood, and are considered to be the main root of distant metastases. (iiarjournals.org)
The easier access for multiple sampling from peripheral blood makes it the method of choice. (iiarjournals.org)
CTL's cryopreserved peripheral blood mononuclear cells (PBMC) are ideally suited for T cell monitoring in ELISPOT and ELISA assays, cytokine bead arrays, tetramer and pentamer staining, cytokine capture assays, and any assays that require live PBMC. (immunospot.eu)
This pilot study was conducted to investigate the potential effects of timing of CD4+ T-cell processing from peripheral blood of atopic and non-atopic adults on their transcriptomic and epigenetic profiles. (bvsalud.org)

Human tissue samples represent an invaluable source of information for the analysis of disease-specific cellular alterations and their variation between different pathologies. (nature.com)
In this workshop, learn tips and tricks for preparing cancer tissue samples for a variety of single cell applications. (the-scientist.com)
This Technique Talk will highlight the best practices for preparing cancer tissue samples for optimal target cell recovery in single cell protocols. (the-scientist.com)
However, most GEP studies have typically been performed on whole tissue samples, containing varying degrees of tumor cell content, which results in uncertainties in data analysis. (lu.se)
Formalin‑fixed paraffin embedded tissue samples were used for the mutation analysis and the content of the neoplastic cells was evaluated using light microscopy. (spandidos-publications.com)
A total of 429 tissue samples (83.3%) were obtained by biopsy and the remaining samples were obtained by surgery. (spandidos-publications.com)
A total of 75 tissue samples were analyzed with an average sample weight of 2.9 mg. (aacrjournals.org)
Tissue samples might be collected ante-mortem (per clinical discretion and after obtaining patient consent) and/or post-mortem (if permission for autopsy). (cdc.gov)

ABSTRACT In January 2002, a pilot programme of neonatal screening for sickle cell disease was launched in the United Arab Emirates (UAE) in 3 districts of Abu Dhabi emirate. (who.int)

Clinical features of the syndrome depend on tumor cell type. (medscape.com)

The analysis of complex biological samples is an important challenge in many bioanalytical and clinical applications. (confex.com)
B cell receptor (BCR) signaling plays a critical role in driving proliferation and survival of the malignant clone in chronic lymphocytic leukemia (CLL), supported by the clinical activity of inhibitors targeted toward BCR-associated kinases [ 1 ]. (nature.com)
Estimated time burden of collection of clinical data and samples, per participant, in minutes. (cdc.gov)
Sickle cell disease: clinical features and management. (medlineplus.gov)
Although nonfunctional islet cell tumors are not uncommon at autopsy, most islet cell tumors with clinical manifestations are functional. (medscape.com)

Cells;11(19)2022 09 22. (bvsalud.org)

2004) Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib. (scielo.br)

Nowadays, a huge body of literature describes CTCs as cells that, after detaching from the primary tumour and circulating through the blood or lymphatic system, are the main cause of the occurrence of metastases in patients with epithelial cancer ( 2 - 4 ). (iiarjournals.org)
Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)
NRG1α was detected in serous cells within normal submucosal glands and in epithelial cells lining honeycombing areas in IPF, and was not detected in other patients. (bmj.com)
Conclusion Expression of SOX2 and MUC5B and lack of SPDEF in atypically differentiated cells of bronchiolised distal airspaces are consistent with abnormal programming of airway epithelial cells in IPF. (bmj.com)

Cumulative incidence of KI polyomavirus (KIPyV) and WU polyomavirus (WUPyV) detection after transplantation in 222 hematopoietic cell transplantation recipients. (cdc.gov)
Many limited samples used in the Low Cell ChIP Kit will be below the limit of detection for instruments commonly used for DNA quantification (e.g. (activemotif.com)
Numerous studies suggest that detection of CTCs in the blood or disseminated tumour cells (DTCs) in the bone marrow of patients with cancer is linked to poor outcomes of the disease and reduced treatment efficiency ( 5 , 6 ). (iiarjournals.org)
Therefore efforts should be made to improve detection efficiencies from these samples. (iiarjournals.org)
When the end point of the chemical reaction is reached, which is shown by an inflection point on the titration curve using a specified detection system, the amount of titrant required is used to generate a result which is reported in milligrams of potassium hydroxide equivalent per gram of sample (mg of KOH/g). (wikipedia.org)

Fig. 6: Cross-method validation of brain TIME and different isolation methods provide distinct advantages for the extraction of immune cells from brain tumor tissue. (nature.com)
The polyP positive cells were recovered by FACS, purified by colony isolation and phylogenetically identified by 16S rRNA gene sequencing. (frontiersin.org)
However, methods to analyze accumulation of specific compounds in individual living cells from a community, followed by recovery and isolation, are still limited. (frontiersin.org)
Optimal pre-analytical conditions for blood sample processing and isolation of selected cell populations for subsequent transcriptomic and epigenomic studies are required to obtain robust and reproducible results. (bvsalud.org)

Using the Low Cell Optimization Module, a qPCR-based quantification of samples can be performed following chromatin preparation. (activemotif.com)
ICP-OES is a well established method for identification and quantification of elements in solid and aqueous samples but lacking information about the chemical nature of the element in the sample if not used in combination with a hyphenated separation technique. (tuwien.at)

This experiment will allow us to study the effects of microgravity on muscle cell biology. (adventistworld.org)
Since SLAMF receptors play a role in NK cell biology, we reasoned that these receptors may impact NK cell-mediated CLL immunity. (nature.com)

Single cell sequencing experiments allow scientists to measure differences in gene expression to characterize cell types and quantify cell population heterogeneity. (the-scientist.com)
Transcription factors (TFs) are critical for B-cell differentiation, affecting gene expression both by repression and transcriptional activation. (lu.se)
The team will study the gene expression changes in the cells sent to space compared to the cells that remained on earth. (adventistworld.org)
Results: Cytokeratin gene expression increased in all samples, when as few as 10 tumour cells were added. (iiarjournals.org)
In this regard, we used blood samples from a healthy donor mixed with different numbers of established breast cancer cells (CAMA-1, MCF7, ZR-75-1) and carried out subsequent gene expression analysis for cytokeratins 8, 18 and 19. (iiarjournals.org)

Fig. 5: Immune cell yield and activation in brain (tumor) tissues differs across tissue dissociation methods. (nature.com)
We investigated the expression of immune checkpoints (ICs) on lymphoid populations within the CRC TME and compared with cells from normal colon tissues using samples from 50 patients with varying disease stages. (mdpi.com)
In this work, pathways governing mucus cell differentiation were investigated in lung tissues from patients with IPF and other chronic pulmonary disorders. (bmj.com)
The Translational Genomics Research Institute received a grant to develop a standard for the "processing and storage of solid tissues for single-cell RNA sequencing", compared to the typical practice of relying on the average of sequencing multiple cells. (wikipedia.org)

Mucus cells lining abnormal airways from patients with IPF expressed MUC5B and SOX2 but lacked SAM pointed domain containing ETS transcription factor (SPDEF) and forkhead box A3(FOXA3). (bmj.com)
The red blood cells deform because they contain an abnormal type of hemoglobin , called hemoglobin S, instead of the normal hemoglobin, called hemoglobin A. (healthwise.net)
Depending on the other type of abnormal hemoglobin, these people may have mild or severe sickle cell disorder. (healthwise.net)
In sickle cell trait, more than half of the hemoglobin is normal (hemoglobin A) and less than half is abnormal (hemoglobin S). (healthwise.net)
The sickle cell test looks for the abnormal hemoglobin in the blood that causes the disorder sickle cell disease . (medlineplus.gov)
This test is done to tell if a person has abnormal hemoglobin that causes sickle cell disease and sickle cell trait. (medlineplus.gov)
This means the person might have the abnormal hemoglobin for sickle cell, but these other factors are making their test results appear negative (normal). (medlineplus.gov)
The sickle-cell gene has become common in Africa because the sickle-cell trait confers some resistance to falciparum malaria during a critical period of early childhood, favouring survival of the host and subsequent transmission of the abnormal haemoglobin gene. (who.int)

Follow-up analysis confirmed that these isolates accumulate polyP, indicating that DAPI can be implemented in staining living cells and FACS can effectively and rapidly screen and isolate individual cells from a complex microbial community. (frontiersin.org)

Conclusion: By this methodology, cells from mammary carcinoma cell lines can be detected in blood samples. (iiarjournals.org)

This method is based on the immunomagnetic enrichment of CTCs from patient's blood samples, with subsequent staining of these cells. (iiarjournals.org)

The high throughput was achieved by either a sample pooling or a cassette dosing approach, along with the use of a rapid, simple and sensitive direct injection/on-line guard cartridge extraction/tandem mass spectrometric assay that was also developed in this study. (nih.gov)

These replicate into multiple colonies of the infectious form, known as elementary bodies, which are then released during cell lysis to start the cycle again. (medscape.com)

There were instances in which sperm cells could not be recovered, because they were out of cage. (ojp.gov)

In this study, global GEP analyses were performed on highly purified, flow-cytometry sorted tumor-cells from eight subgroups of BCLs. (lu.se)
The last stage in the process of measuring the percentage of CD4+ T-lymphocytes in the whole-blood sample is referred to as 'immunophenotyping by flow cytometry' (22-28). (cdc.gov)
The CDC guidelines concerning CD4+ T-cell determinations (33) were first published in the MMWR in 1992 to provide laboratorians with the most complete information about how to measure CD4+ T-lymphocytes in blood from HIV-infected persons by using flow cytometry. (cdc.gov)

In April 2018, the first data set from the project was released, representing 530,000 immune system cells collected from bone marrow and cord blood. (wikipedia.org)

Sickle cell disease is an inherited blood disease that causes red blood cells to be deformed ( sickle-shaped ). (healthwise.net)
The main problems arise from the tendency of the red blood cells to become sickle-shaped and block capillaries at low oxygen tension. (who.int)
In children, sickle-shaped red blood cells often become trapped in the spleen, leading to a serious risk of death before the age of seven years from a sudden profound anaemia associated with rapid splenic enlargement or because lack of splenic function permits an overwhelming infection. (who.int)

DNA standards and GAPDH-2 primers were used to analyze DNA from Fixed Jurkat Cell Pellet samples (equivalent to 100,000 Jurkat cells). (activemotif.com)
Nonetheless, there a need to further analyze if these systems can adequately separate samples more closely related to those found naturally in biological settings, such as blood samples. (confex.com)
We focused on validating this method in a model system, since this is a necessary step before starting to analyze patient samples. (iiarjournals.org)

However, distant non-malignant lung samples had significantly higher Cd levels compared to primary lung tumor (p=0.013). (aacrjournals.org)
Indeed, our experiments showed significantly decreased degranulation capacity of primary NK cells from CLL patients expressing low levels of SLAMF1 and SLAMF7. (nature.com)
We found that the levels of B cells, T cells, and NK cells were similar, IC-expressing CD4 + and CD4 + CD8 + double positive T cells were higher, while CD8 + T cells and CD4 − CD8 − double negative T cells were significantly lower in CRC tumors. (mdpi.com)
A pattern of reimbursements that deviates significantly from a normal course of cell phone use in the employerâ s business (e.g., an employee received reimbursements for cell phone use of $100/quarter in Q1, Q2, Q3, but receives a reimbursement of $500 in Q4). (proyectosmetalicos.es)

The protocol maximizes the information yield from limited tissue and includes both the preservation of bulk tissue, which can be performed within 1 h following surgical resection, as well as tissue dissociation for an in-depth characterization of individual TIME cell populations, which typically takes several hours depending on tissue quantity and further downstream processing. (nature.com)
In sub-Saharan Africa mortality will be much higher, and in some areas estimates derived from the age structure of populations attending clinics suggest that half of those with sickle-cell anaemia have died by the age of five years usually from infections including malaria and pneumococcal sepsis, and from the anaemia itself. (who.int)

Of particular interest has been the use of single-cell genomics to study cell fate decisions in haematopoeisis. (bioconductor.org)

When analyzing tissue for oncology research, samples must transform into clean single cell suspensions free of debris, aggregates, and dead cells for best results. (the-scientist.com)
The present study will focus on the selective capture, enrichment, and separation of intact biological cells from samples containing cell debris and cell organelles. (confex.com)

In this application note, RETSCH GmbH discusses the importance of sample preparation for various biological materials. (selectscience.net)
RETSCH offers a range of mills and grinders for pulverization, cell disruption, and homogenization of biological samples. (selectscience.net)
The research activity, performed at the institute for Microelectronic and Microsystems, has been devoted to the study and the development of non-destructive optical techniques for morphological analysis of biological samples. (unina.it)
A digital holographic microscope has been designed and set up, compatible with micro-manufactured devices, for a label-free, non-destructive characterization of biological samples, as particular regard to bovine and human spermatozoa. (unina.it)
Moreover, dedicated algorithms have been developed in order to elaborate the acquired hologram and to retrieve the needed biological parameters of the analyzed sample. (unina.it)

The impact of the type and quantity of malignant cells of the neoplastic specimen on the quality of mutation analysis remains to be elucidated, and only empirical and sporadic data are available. (spandidos-publications.com)
Islet cell tumors in this group are often multiple and malignant. (medscape.com)

This first-of-its-kind module includes 'positive control' samples and antibody, as well as reagents to examine chromatin quantity and quality following sonication and ChIP enrichment. (activemotif.com)
It is important to maximize retainment of limited samples throughout the ChIP protocol, which is why Active Motif has developed a module to examine samples at intermediate steps in the workflow: following chromatin preparation and ChIP enrichment (see purple steps on Days 1 and 4 below). (activemotif.com)
Early examination of fragmentation allows users to optimize sample sonication efficiently, without wasting time and reagents on enrichment and library preparation. (activemotif.com)
To ensure specific and successful antibody enrichment of your sample, we recommend using primers targeted towards positive and negative control regions to amplify and characterize fold-enrichment. (activemotif.com)

Agar gel, cellulose acetate, and starch gel electrophoresis are all capable of diagnosing sickle-cell anaemia, sickle-cell haemoglobin C disease, and haemoglobin C disease in cord blood samples. (bmj.com)
Paper electrophoresis can reliably and rapidly detect sickle haemoglobin and haemoglobin C in cord blood samples. (bmj.com)

Cell-free RNA (cfRNA) transcripts known to be expressed by the fetal brain are detectable by quantitative reverse transcription PCR (RT-qPCR) in amniotic fluid and represent potential biomarkers of neurodevelopment. (biomedcentral.com)
There appears to be no benefit in collecting AF in Streck RNA BCT for quantitative studies of AF cell-free RNA. (biomedcentral.com)

The end point is detected using a glass electrode which is immersed in an aqueous solution containing the sample, and connected to a voltmeter/potentiometer. (wikipedia.org)

Metz H, Busch SE, Hanke ML, Kargl J, Kim KH and Houghton M (2014) Insulin receptor substrate-1 regulates immune cell content in lung adenocarcinoma. (scielo.br)

The authors sought to identify mechanisms driving the differentiation of mucus cells during the bronchiolisation process. (bmj.com)
Methods Pathways governing airway mucus cell differentiation include SRY (sex determining region Y)-box 2 (SOX2), Notch, forkhead box A3(FOXA3)/SAM pointed domain containing ETS transcription factor (SPDEF), epidermal growth factor (EGF) and the EGF-related neuregulins NRG1α and NRG1β. (bmj.com)
Neuregulin1α, which drives mucus cell differentiation in vitro, was expressed in normal airway submucosal glands and in lungs from patients with IPF. (bmj.com)

This is problematic for DE analyses between conditions, as DEGs detected for a particular cell type may be driven by differences in the ambient profiles rather than any intrinsic change in gene regulation. (bioconductor.org)
This applies to both the sample preparation and data analyses. (ojp.gov)

As early as 1869, cells with the morphology of cancer cells had been found by Thomas Ashworth in the blood of a person who died from metastatic cancer ( 1 ). (iiarjournals.org)

However, each tissue type is unique, so scientists should optimize their sample preparation protocols prior to starting any single cell experiment. (the-scientist.com)
Differences in the ambient profile across samples are not uncommon when dealing with strong experimental perturbations where strong expression of a gene in a condition-specific cell type can "bleed over" into all other cell types in the same sample. (bioconductor.org)
The aim of the present study was to investigate the impact of tissue type and content of neoplastic cells in the specimen on the quality of EGFR mutation analysis among patients with lung adenocarcinoma. (spandidos-publications.com)
Signaling Lymphocytic Activation Molecule Family (SLAMF) receptors are a group of nine type I transmembrane receptors that are mainly expressed on a variety of immune cells. (nature.com)
Type 40T TempTrol™ Polarimeter Sample Cells: These cells are constructed of stainless steel and Delrin ® and come with Luer ® fittings at either end of the cell for easy filling with a plastic syringe or pipette. (rudolphresearch.com)
If your application demands 1ml or less, Type 32 Cells offer less than 0.5ml volumes. (rudolphresearch.com)
Rapid operation is achieved by using a Type 31 or 33 Flow Through Polarimeter Sample Cell as seen in Picture C. Once the sample cell is filled, each successive sample poured into the funnel will displace the previous sample through the drain tube. (rudolphresearch.com)
The Human Cell Atlas will catalogue a cell based on several criteria, specifically the cell type, its state, its location in the body, the transitions it undergoes, and its lineage. (wikipedia.org)

Also, our mutants induced proliferation, glucose uptake, inhibited the migration of 293T cells and affected the responsiveness of the cells to cisplatin and radiation. (scielo.br)
Overexpression of SLAMF1 or SLAMF7 in IGHV mutated CLL cell models resulted in reduced proliferation and impaired responses to BCR ligation, whereas the knockout of both receptors showed opposing effects and increased sensitivity toward inhibition of components of the BCR pathway. (nature.com)

The most sober explanation is that the background samples contain more hemoglobin transcripts in the ambient solution due to leakage from erythrocytes (or their precursors) during sorting and dissociation. (bioconductor.org)
In sickle cell disease, almost all hemoglobin is hemoglobin S with some hemoglobin called hemoglobin F . (healthwise.net)
Hemoglobin is a protein in red blood cells that carries oxygen. (medlineplus.gov)

Using this module, researchers can monitor low cell samples throughout the ChIP protocol, identify and optimize steps responsible for sample loss, and save time and money by only moving forward with samples that will generate valuable ChIP-Seq data. (activemotif.com)
This module is intended to be used together with the Low Cell ChIP Kit, either to optimize protocol steps prior to using your precious samples, or to troubleshoot the point in the protocol responsible for sample loss. (activemotif.com)
This provides users the ability to optimize workflow conditions prior to using their low input, precious samples. (activemotif.com)

The capacity of this iDEP system is evaluated for the analysis of samples containing low concentrations of the desired intact cells in mixtures with high concentrations of cell lysate. (confex.com)
However, the apparent permeability coefficients of the test drugs across Caco-2 monolayers measured by the sample pooling or cassette dosing (up to five drugs co-administered in this study) strategy were in good conformity with the data obtained by single-drug dosing followed by discrete sample analysis. (nih.gov)
The sample sheet is used by analysis software after the sequencing. (lu.se)
Since cyanide forms volatile hydrogen cyanide gas, tissue sampling techniques, storage, and cyanide analysis must be done with caution. (cdc.gov)

This is the Final Technical Report on the findings and methodology of a study that examined whether and how an automated dielectrophoretic-based single cell separation technique (DEPArray) can improve forensic laboratory efficiency, mixture deconvolution, and combat sample inhibition. (ojp.gov)
The implementation of a separation cell using an acoustic standing wave removes the centrifugation step from the conventional DPE method, therefore making it automatable and suitable for on-line application. (tuwien.at)
Experiments indicated that this method allows continuous and stable measurements without the need for changing syringes, and without disturbing the flow within the separation-cell. (tuwien.at)

When health impact is measured by under-five mortality, sickle-cell anaemia contributes the equivalent of 5% of under-five deaths on the African continent, more than 9% of such deaths in west Africa, and up to 16% of under-five deaths in individual west African countries. (who.int)

This study involved approximately 100 DEPArray runs, including those that focused on training, optimization, and experimental samples. (ojp.gov)
Combining the experimental procedure for phenotyping living cells in a microbial community with the cell recovery necessary for further cultivation will allow for an efficient initial screening process. (frontiersin.org)

Therefore, we recommend a qPCR-based approach to examine samples following chromatin preparation, to ensure that you have retained enough sample to proceed through the protocol. (activemotif.com)
The Low Cell Optimization Module allows sample fragmentation profiles to be examined following chromatin preparation (Day 1), rather than following NGS library preparation (Day 4). (activemotif.com)

Pancreatic islet cell tumors may secrete 2 or more polypeptide hormones. (medscape.com)
This article reviews the role of imaging in the management of islet cell tumors. (medscape.com)
No universally agreed-upon algorithm exists in the radiologic investigation of pancratic neuroendocrine (islet cell) tumors of the pancreas. (medscape.com)

This enabled identification of TFs that can be uniquely associated to the tumor cells of chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), hairy cell leukemia (HCL), and mantle cell lymphoma (MCL). (lu.se)

The RoToR is revolutionising genetic, genome and cancer research by being able to manipulate over 200,000 yeast or bacteria cell samples per hour - an order of magnitude faster than previous lab automation . (dpaonthenet.net)
The note highlights the use of RETSCH mills for applications such as cell disruption of yeasts, microalgae, and bacteria, homogenization of soft and tough samples like sputum or liver, cryogenic grinding, and pulverization of forensic samples. (selectscience.net)

These positive control reagents, not provided with any other low cell ChIP kit on the market, save users the frustration of optimizing and troubleshooting protocols with samples they care about. (activemotif.com)

Materials and Methods: Blood samples of a healthy donor were mixed with specific numbers of cells from different breast carcinoma cell line cells. (iiarjournals.org)
Methods: Tissue collected from treatment-naïve stage I and II NSCLC patient samples that underwent surgical resection was analyzed by inductively coupled plasma mass spectroscopy (ICP-MS) for concentration of Cd, As, Hg, and Pb. (aacrjournals.org)
Many of the analytical methods used for environmental samples are the methods approved by federal agencies and organizations such as EPA and the National Institute for Occupational Safety and Health (NIOSH). (cdc.gov)

In addition to HIV antibody testing in NHANES, whole-blood samples were collected and stored for future CD4 testing once the HIV status of the sample was known. (cdc.gov)
Using immunophenotyping, HIV-positive blood samples and age-matched controls were tested for the proportion of lymphocytes that are T cells, B cells, natural killer (NK) cells, CD4+ T cells (helper T cells), and CD8+ T cells (suppressor/inducer T cells). (cdc.gov)
Lesion swabs, mucosal swabs, and blood samples will be collected at each visit including enrollment. (cdc.gov)
Two heparinized blood samples were drawn from each of three atopic and three healthy individuals. (bvsalud.org)
Participants completed survey questionnaires assessing subjective smoking experience, exhaled carbon monoxide (eCO) testing, and provided blood samples for monitoring plasma nicotine. (who.int)

Scientists have investigated how pig brain cells behave after the animal has died. (newsweek.com)
Scientists have revived the brain cells of dead pigs in a study that experts say calls into question our understanding of what makes an animal alive. (newsweek.com)
After pumping the brains with the liquid for six hours, the scientists noticed the activity of some molecules, cells and synapses were restored. (newsweek.com)

We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote p.S668T mutation in nine of 42 samples and four of nine also had the p.D674H mutation. (scielo.br)
Lung cancer is divided into two major groups: non small cell lung cancer (NSCLC) and small cell lung cancer (SCLC). (scielo.br)
We surveyed tissue from current/former smokers with non-small cell lung cancer (NSCLC) for heavy metal content. (aacrjournals.org)

Dermabrasion versus microneedling in transplantation of autologous noncultured melanocyte-keratinocyte cell suspension in patients with vitiligo. (medscape.com)

HIV infection is characterized by a decrease and, eventually, a depletion of CD4+ T-lymphocytes (helper T cells). (cdc.gov)
Accurate and reliable measures of CD4+ T-lymphocytes (CD4+ T-cells) are essential to the assessment of the immune system of human immunodeficiency virus (HIV)-infected persons (1-3). (cdc.gov)
and the percentage of lymphocytes that are CD4+ T-cells. (cdc.gov)

Currently, only one methodology for the discovery and enumeration of CTCs is approved, at least for the metastatic setting by the US Food and Drug Administration (FDA), namely the so-called Cell Search® System ( 10 ). (iiarjournals.org)
Although flow cytometric immunophenotyping is a highly complex technology, methodology for performing CD4+ T-cell determinations has become more standardized between laboratories. (cdc.gov)

For precious samples and essential oils that have a large optical rotation, cells with only 0.05ml volumes and 10mm optical path lengths are available. (rudolphresearch.com)

Accurate sample fragmentation is integral for high quality ChIP-Seq data. (activemotif.com)
A prior study has demonstrated that prolonged storage of AF at −80 °C leads to fragmentation of the cell-free nucleic acids [ 4 ]. (biomedcentral.com)

the aim of the experiment was to determine if blocking of the erythroid lineage diverted cells to other developmental fates. (bioconductor.org)
Muscle cells from research-study participants at Florida Hospital's Translational Research Institute for Metabolism and Diabetes (TRI) will be blasting off toward the International Space Station in mid-November 2018 as part of an experiment to examine the effects of a weightless environment on muscle health. (adventistworld.org)
On June 11, ESA astronaut Thomas Pesquet will be installing sample cells for a new experiment, FOAM-C, for the Belgian space control centre (B.USOC) on board the International Space Station. (aeronomie.be)

2019 ) . This is very similar to the WT chimera dataset we previously examined, only differing in that the Tal1 gene was knocked out in the injected cells. (bioconductor.org)

This is due to the quality of the cell and inability of the cell to respond properly to the dielectrophoresis. (ojp.gov)

Together, our data indicate that SLAMF receptors are important modulators of the BCR signaling axis and may improve immune control in CLL by interference with NK cells. (nature.com)
The sample sheet is a tab-separated text file with data about the samples that are put into a flow cell, including information about barcoding. (lu.se)
The Tabula Sapeins data was published on a dedicated website List of distinct cell types in the adult human body Human Genome Project ENCODE - Encyclopedia of DNA Elements (ENCODE) Human Protein Atlas Preidt 2016. (wikipedia.org)
Larger sample studies are needed to provide more detailed data regarding use of CG for treatment of vitiligo. (medscape.com)
There are, however, no firm data on the survival of patients with sickle-cell anaemia on the African continent. (who.int)

A total of 456 samples (88.5%) were observed from primary lung adenocarcinomas, while 59 (11.5%) were from metastatic lesions. (spandidos-publications.com)
2004) Akt phosphorylation and gefitinib efficacy in patients with advanced non-small-cell lung cancer. (scielo.br)
Translational Inflammation Research Division & Core Facility for Single Cell Multiomics, Medical Faculty, Philipps University of Marburg, Member of the German Center for Lung Research (DZL) and the Universities of Giessen and Marburg Lung Center, D-35043 Marburg, Germany. (bvsalud.org)

Squamous cell carc1

Genes12

HPLC3

Fluorescence8

Venous1

Viability2

Biopsies1

Peripheral4

Tissue samples8

Abstract1

Tumor cell1

Clinical5

20221

Epidermal growth f1

Epithelial4

Detection5

Isolation4

Quantification2

Biology2

Gene expression5

Tissues4

Abnormal8

FACS1

Mammary1

Subsequent1

Assay1

Lysis1

Sperm cells1

Flow-cytometry3

Bone marrow1

Sickle-shaped3

Analyze3

Significantly4

Populations2

Genomics1

Debris2

Biological5

Malignant2

Enrichment4

Cord blood2

Quantitative2

Aqueous1

Content in lung1

Differentiation3

Analyses2

Morphology1

Type8

Proliferation2

Hemoglobin3

Optimize3

Analysis4

Separation3

Equivalent1

Experimental2

Preparation2

Tumors3

Chronic lymphocy1

Bacteria2

Protocols1

Methods3

Blood samples5

Scientists3

NSCLC3

Autologous1

Lymphocytes3

Methodology2

Precious samples1

Fragmentation2

Experiment3

20191

Dielectrophoresis1

Data5

Lung3