Sample concentration and separation. Medical search. Frequent questions

Chromatographic separation is achieved using an IonPac AS16 column with sodium hydroxide as the eluent. (cdc.gov)
The GC column is the heart of a GC system, where chromatographic separation occurs. (sigmaaldrich.com)
The method consists of extraction of soil samples with methanol, filtration, liquid chromatographic separation of methanol-soluble components using a C18 column and UV detection at 275 nm. (epa.gov)
Inline gamma-spectrometry of fission product elements after rapid high-pressure ion chromatographic separation. (ornl.gov)
Chromatographic separation of nalbuphine and naloxone (internal standard) was achieved on a Phenomenex Kinetex PFP (2.6 μm, 100 A, 100 × 2.1 mm) column using a mobile phase consisting of 0.1% formic acid, 15 mM ammonium acetate in deionized water and acetonitrile (60:40, v/v). The flow rate was 0.3 mL/min and the total run time was 2 min. (unboundmedicine.com)

On the other hand, recent rapid nucleic acid-based diagnostic methods are prone to false positives from human DNA mainly due to a lack of efficient sample preparation methods. (kth.se)
This Ph.D. work was aimed at the development of novel sample preparation methods for rapid and efficient separation and identification of bacteria from blood for sepsis diagnostics. (kth.se)
All in all, the ability of these sample preparation methods for rapid and effective separation and detection of key pathogens in blood will help in decreasing the time of sepsis diagnosis and aid towards efficient phenotypic or genotypic analysis. (kth.se)
The goal was to evaluate the effect of (a) dissolving methods, (b) sampling separation, and (c) quantification technique on the accuracy of solubility data. (aiche.org)
This Afghanistan standard prescribes requirements, methods of sampling and tests for fortified edible fats and oils intended for human consumption. (who.int)
Analyzing complex protein samples using traditional two-dimensional electrophoresis methods is tedious and time-consuming. (thermofisher.com)
The method might be less likely to compete as directly with label-free CTC capture methods - like Vortex Biosciences' VTX-1 , Angle's Parsortix , or the CTC iChip - which rely on fluidic separation and concentration of CTCs based on their size, shape, or malleability, pulling out a larger population of cells for analysis, regardless of whether they carry a particular genomic target. (genomeweb.com)
Three-tube most-probable-number (MPN) assays and anti-Salmonella immunomagnetic separation methods were utilized to determine the levels of Salmonella and aid with the recovery of Salmonella species, respectively. (nih.gov)
These methods have the disadvantage of completely destroying the sample, and any species contained within it. (newworldencyclopedia.org)
Analytical methods rely on scrupulous attention to cleanliness, sample preparation, accuracy and precision. (newworldencyclopedia.org)
The quantification of gold nanoparticles (AuNP) in environmental samples at ultratrace concentrations can be accurately performed by sophisticated and pricey analytical methods. (muni.cz)
Small volumes of sample (5 mL), dispersive solvent (50 mu L), and extraction agent (70 mu L) were reported also for surfactant-assisted dispersive liquid-liquid microextraction (SA-DLLME) coupled with electrothermal vaporization inductively coupled plasma mass spectrometry (ETV-ICP-MS). The limits of detection (LOD) achieved using different combinations of methods as well as enrichment factors (EF) varied greatly, being 0.004-200 ng L-1 and 8-250, respectively. (muni.cz)
However, the major challenge with MS-based methods is the large concentration difference between HCPs (usually in low ppm levels) and the target protein (high concentration). (pharmiweb.com)
To address this challenge, two general strategies are available: the addition of separation methods such as 2D-LC and/or ion mobility to remove interfering peptides and improve separation efficiency, or focusing on sample preparation by enriching HCPs through affinity purification or molecular weight cut-off (MWCO) ultrafiltration. (pharmiweb.com)
Petrographic studies and beneficiation methods including gravity concentration, froth flotation, and magnetic separation were used to determine mineral association and to attempt to concentrate the platinum values with a primary mineral value such as chromite, copper sulfide, or magnetite. (cdc.gov)

Comparison of relative response factors (ratio of native analyte to stable isotope labeled internal standard) with known standard concentrations yields individual analyte concentrations. (cdc.gov)
In GC, an analyte is injected into the instrument's sampling port and enters an oven where it is vaporized. (sigmaaldrich.com)
Mass spectrometry is used to determine the molecular mass, the elemental composition, structure and sometimes amount of chemical species in a sample by ionizing the analyte molecules and observing their behavior in electric and magnetic fields. (newworldencyclopedia.org)

Compounds in the sample partition between the column's stationary phase and the carrier gas. (sigmaaldrich.com)
Many samples can be analyzed by GC so long as the compounds are reasonably volatile (can be vaporized) yet are thermally stable (do not degrade at high temperature). (sigmaaldrich.com)
The purpose of this work was to review the options for the characterization of HILIC stationary phases and their applications for separations of polar compounds in complex matrices. (springer.com)
NP-LC has been widely used to separate various compounds, from nonpolar to highly polar compounds (note that chromatography was first introduced as a method used in separation science). (springer.com)
Hydrophilic interaction liquid chromatography has established itself as the separation mode of choice for uncharged highly hydrophilic and amphiphilic compounds that are too polar to be well retained in RP-LC but have insufficient charge to allow effective electrostatic retention in ion-exchange chromatography. (springer.com)
Ten out of the previous eleven model compounds could be quantified in the oxidized humic acid sample. (lu.se)

Applied pressure overcomes osmotic pressure and forces solvent to move from a high solute concentration to a low solute concentration. (sigmaaldrich.com)
Ultrafiltration using Amicon® Ultra centrifugal devices for concentrating virus in preparation of high titer virus stocks and isolation of virus from samples. (sigmaaldrich.com)
The Mn-microparticles contained particularly high amounts of manganese, which accounted for a mean 42% of the total manganese in the oxic sediment samples ( Figure 4 ). (go.jp)
The concentration was determined by three quantification techniques: (1) off-line gravimetrically, or (2) off-line high liquid chromatography method (HPLC) or (3) on-line Raman method. (aiche.org)
The list of places where microplastics are found in high concentrations gets longer every month, making the analysis of microplastic pollution a challenging, but very important task. (bruker.com)
We get very high efficiencies in spiked samples where we are capturing both higher EPCAM and lower EPCAM cells. (genomeweb.com)
If the concentration of element or compound in a sample is too high for the detection range of the technique, it can simply be diluted in a pure solvent. (newworldencyclopedia.org)
Sodium borohydride is used to produce arsine gas, but high metal concentrations can suppress arsine production. (usgs.gov)
Separation of interfering metal cations using cation-exchange prior to hydridegeneration permits accurate arsenic(III) determinations in acid mine waters containing high concentrations of interfering metals. (usgs.gov)
Mass spectrometers often struggle to identify and detect low concentrations of HCPs amidst high concentrations of target proteins. (pharmiweb.com)
high concentrations of insulin thus lower serum potassium concentration. (msdmanuals.com)
Calcium concentration, both total and free, is characterized by a high physiological variation, depending on age, sex, physiological state (eg, pregnancy), and even season (owing to the seasonal variation of vitamin D, which is directly involved in the regulation of calcium concentration). (medscape.com)
and their products play an important role in A total of 257 samples of ultra-high the food supply, job generation and income for temperature (UHT) treated milk (n=125), society. (bvsalud.org)
The samples were collected at random according view of its high nutritional value and represents to market availability by local health offices a natural source of carbohydrates, proteins, (state and municipal) and sent to the laboratory fats, vitamins and minerals in different dispersion properly cooled, when it is required. (bvsalud.org)
In the presence of high concentrations of inert macromolecules, the self-association of proteins is strongly enhanced through an entropic, excluded-volume effect variously called macromolecular crowding or depletion attraction. (lu.se)
The high separation power and short analysis time obtained demonstrate for the first time that supercritical fluid chromatography is a fast and reliable technique for the analysis of lignin-derived phenols in complex environmental samples. (lu.se)
However, less recognized by many employers and workers is that many of the chemicals found in volatile hydrocarbons are acutely toxic at high concentrations. (cdc.gov)
Volatile hydrocarbons can affect the eyes, breathing, and the nervous system (3,4,5,6,7) and at high concentrations may also affect the heart causing abnormal rhythms (8,9). (cdc.gov)
5) Use appropriate respiratory protection in areas where potentially high concentrations of volatile hydrocarbons can occur as an interim measure until engineering controls are implemented. (cdc.gov)

Filtration is used in dissolution testing to end the dissolution process and prepare the sample for HPLC analysis. (sigmaaldrich.com)
Watch our step-by-step video to understand how the Samplicity ® G2 filtration system works and use our application notes to learn more about how the Samplicity ® G2 filtration system has been applied for HPLC sample preparation. (sigmaaldrich.com)
The reason for the standard deviation difference is probably due to the multiple-step sample dilution procedure for HPLC. (aiche.org)
Modern separation techniques such as HPLC often seek to separate and determine amount or identity in a single automated analysis by integrating a detector. (newworldencyclopedia.org)
In the in-chemico direct peptide reactivity assay (DPRA), test item solutions were tested by incubating the samples with the peptides containing either cysteine or lysine for 24 ± 2 h at 25 ± 2.5 °C. Subsequently samples were analysed by HPLC. (europa.eu)
After the 24 h ± 2 h incubation period but prior to the HPLC analysis samples were inspected for precipitation, turbidity or phase separation. (europa.eu)

If the amount in the sample is below an instrument's range of measurement, the method of addition can be used. (newworldencyclopedia.org)

Filtration is a separation technique used to concentrate or purify substances based on their physical or chemical properties. (sigmaaldrich.com)
Filtration is used to reduce sample complexity, improve clarity of viscous samples, and reduce background signals resulting in increased signal-to-noise ratios in analytical tests. (sigmaaldrich.com)
Through analysis of means, there were no significant differences between using centrifugation or filtration as the sampling separation method. (aiche.org)
The filtration method cannot be used in a silty environment with silt concentrations larger than about 50 mg/1 because of rapid filter blocking by the fine silt particles. (coastalwiki.org)

In GC, external and internal standards are commonly employed in order to ensure reliable quantification of the test sample. (sigmaaldrich.com)
We discuss several combinations of techniques that are suitable for separation/preconcentration and quantification of AuNP in complex and challenging aqueous matrices, such as tap, river, lake, brook, mineral, and sea waters, as well as wastewaters. (muni.cz)

Achieve better gas chromatography separations with an appropriate column. (sigmaaldrich.com)
For historical reasons, it has been reported that HILIC is a variant of normal phase liquid chromatography, but the separation mechanism used in HILIC is more complicated than that in NP-LC. (springer.com)
Figure 2 shows how HILIC complements other areas of chromatography and extends the range of separation options. (springer.com)
The first step may also involve a separation technique, such as chromatography , and the second a detection / measuring device. (newworldencyclopedia.org)

The second dimension consists of a conventional SDS-PAGE electrophoretic separation where proteins are differentiated based on their molecular weights. (thermofisher.com)
Through a separation process developed by Barreto, a liquid concentration packed with the patient's own inflammation-fighting and growth proteins is created. (i2e.org)
Unless serum proteins contain abnormalities, total serum calcium concentration is normally between 8.5 and 10.2 mg/dL of serum. (medscape.com)
Because ionized calcium is the only component of the total serum calcium level that is regulated by calciotropic hormones, decisions on the total serum calcium concentration should not be made unless changes in concentrations of plasma proteins, particularly albumin , are considered. (medscape.com)

This data release contains eight datasets and metadata related to streamwater constituent load estimation and E. coli bacteria concentration predictions at 15 watersheds in DeKalb County, Georgia for 2012 to 2016 (the water-quality model calibration data goes through 9/22/2017 and the water-quality assurance samples goes through 11/7/2017). (usgs.gov)
A standard method for analysis of concentration involves the creation of a calibration curve. (newworldencyclopedia.org)

To better characterize micrometer-scale sedimentary microstructures in the entirely oxic SPG sediments, JAMSTEC and Kochi University researchers investigated the ultra-fine structures of marine sediment core samples of various oxidation states, ranging from continental margins to an open-ocean gyre. (go.jp)
By measuring the absorption or emission of light by a substance we can calculate the amounts of species or characterize the chemical species, often without separation. (newworldencyclopedia.org)
to review the experience with lamotrigine therapeutic drug monitoring in a tertiary epilepsy centre aiming to characterize the plasma concentrations profile. (bvs.br)

After taking a sample, the filter system is opened and the filter material with the sand catch is removed and returned to the laboratory for drying, weighing and size analysis. (coastalwiki.org)
To determine the overall accuracy of the pump-filter method, some concentration measurements were carried out in a laboratory flume using a siphon sampler for comparison. (coastalwiki.org)
To determine the sampling efficiency of the pump-bottle method, laboratory measurements were carried out in a flume using sand with D 10 = 150 um, D 50 = 220 um and D 90 = 330 um. (coastalwiki.org)
The Bureau of Mines conducted laboratory batch beneficiation tests on samples from four potential platinum resources in southeastern Alaska to concentrate platinum and other precious metals. (cdc.gov)

The table below lists reagents commonly used for sample preparation. (thermofisher.com)

This strategy effectively narrows the dynamic concentration range, significantly improving the sensitivity of detecting low-abundance HCPs. (pharmiweb.com)

In early sepsis, the concentration of bacteria in the bloodstream is typically low, making diagnosis challenging. (kth.se)
The optimized parameters were extended to demonstrate that when bacteria is spiked into diluted blood, blood remains fully focused at the outer wall throughout the channel length while smaller bacteria differentially migrate towards the inner wall for rapid separation. (kth.se)
Using E.coli spiked into the diluted blood sample, bacteria separation is demonstrated at an efficiency of 82 to 90% depending on the blood dilution using a single spiral chip (Paper-II). (kth.se)

Ultrafiltration (macromolecule separation) separates particles and dissolved molecules from fluids based on particle size. (sigmaaldrich.com)
After separation, flowback fluids are typically stored temporarily in tanks (figure 1) or surface impoundments (lined pits, ponds) at the well site. (cdc.gov)

This approach was also further optimized to perform antibiotic susceptibility testing to determine the minimum inhibitory concentration (MIC). (kth.se)

It is a simple and routine method used in many laboratories to remove insoluble particles from solutions and to prepare samples for analysis. (sigmaaldrich.com)
This study presents palladium results obtained in the analysis of biological samples and reference materials using instrumental thermal and epithermal neutron activation analysis (INAA and ENAA). (usp.br)
A wide variety of matrix-specific sample preparation techniques are available to help isolate and concentrate analytes and sample matrices prior to GC analysis. (sigmaaldrich.com)
A bulk sample of a separation material was taken for asbestos (1332214) analysis. (cdc.gov)
Sample preparation is a fundamental step in any protein analysis workflow, but it is even more crucial in 2DE electrophoresis where no universal method exists. (thermofisher.com)
The chemical analysis usually starts with a liquid sample that was prepared differently depending on its origin. (bruker.com)
The first commercial test in this area is marketed by Genomic Health and uses a CTC analysis method developed by Epic Sciences, which does not isolate CTCs, but rather spreads out all of the cells in a blood sample, and uses microscopy and imaging techniques to locate the CTCs and assess protein expression in their nuclei. (genomeweb.com)
Analytical chemistry is the analysis of material samples to gain an understanding of their chemical composition and structure . (newworldencyclopedia.org)
Qualitative inorganic analysis seeks to establish the presence of a given element or inorganic compound in a sample. (newworldencyclopedia.org)
Plan for optimal sample preparations and analysis depending on sample type and aim of the analysis. (lu.se)
Practical parts including protein and peptide sample preparations, deposition with different techniques and the use of chromatographic separations for complex samples prior to mass spectrometry analysis. (lu.se)
A drawback of SSFs is that difficult sampling and complex microbial dynamics pose an obstacle for analysis and predictions. (lu.se)
stored at -20ºC until analysis (not more than one week after sampling). (bvs.br)

Additionally, they developed a new technique for separating Mn-microparticles from sedimentary matrices by combining a sequential density concentration *4 and flow cytometry/particle sorting *5 , which enabled in-depth analyses of the texture and major and trace element compositions of Mn-microparticles. (go.jp)

Geochemical and microbiological studies of sediment core samples *3 from six SPG drill sites at water depths of 3740-5695 m revealed the presence of dissolved oxygen and aerobic microbial communities throughout the sedimentary sequence. (go.jp)

The water-sediment sample is pumped through a filter (see Figure 2) which separates all particles larger than the mesh size of the applied filter material. (coastalwiki.org)
in which: G= dry mass of sediment (mg), V= volume of water sample (l). (coastalwiki.org)

Optimal sampling requires a discharge of about 0.5 1/min (2 liter bottle in 1 minutes). (coastalwiki.org)

After sample collection and preparation, the analytes of interest are separated inside the column and then a detector measures the quantity of the components that exit the column. (sigmaaldrich.com)
from preparation to separation and blotting as well as antibody incubation and, lastly, detection. (ahdiagnostics.se)
The preparation of your samples is the first step in Western blotting. (ahdiagnostics.se)
Whether you are working with cultured cell lines or tissue samples, sample preparation is often a process of trial and error in which you, through troubleshooting, need to choose the right lysis buffers, enzyme inhibitors, and appropriate stoichiometry. (ahdiagnostics.se)
Solid phase extraction SPE is the sample preparation process. (reportsnreports.com)
Preparation of Specimens and Samples for the U.S. National Nuclear Materials Archive. (ornl.gov)

CTDPH reviews new fish tissue data sampled by the US EPA or CT DEP and evaluates whether the current fish consumption advisory needs to be modified to protect public health based on the level of contamination. (cdc.gov)
The objective of this study was to conduct a year-long market survey (1,322 samples) to help bridge the data gaps on the contamination rates and levels of Salmonella on raw poultry by product type (i.e., breast, thighs, drums, wings, and split breast) and production method (conventional versus organic). (nih.gov)
Many practitioners will keep all their glassware in acid to prevent contamination, samples will be re-run many times over, and equipment will be washed in specially pure solvents. (newworldencyclopedia.org)

Thus, relatively small alterations in serum potassium concentration can have significant clinical manifestations. (msdmanuals.com)
In the absence of factors that shift potassium in or out of cells, the serum potassium concentration correlates closely with total body potassium content. (msdmanuals.com)
Once intracellular and extracellular concentrations are stable, a decrease in serum potassium concentration of about 1 mEq/L (1 mmol/L) indicates a total potassium deficit of about 200 to 400 mEq (200 to 400 mmol). (msdmanuals.com)
Total serum calcium is less difficult to measure than the ionized calcium component is, and ionized calcium measurements are rarely needed if serum protein concentrations can be measured. (medscape.com)
In patients multiple myeloma, the globulin concentration is often increased, leading to excessive binding of calcium to the monoclonal paraprotein and occasional elevation of the total serum calcium concentration, yet the ionized calcium level may be normal in these individuals. (medscape.com)

Results from initial field studies suggest that certain flowback operations/activities can result in elevated concentrations of volatile hydrocarbons in the work environment that could be acute exposure hazards. (cdc.gov)
exposure concentrations. (cdc.gov)

The article presents the methodology of preparing a cosmetic sample for analysi, and the creation of a dataset for teaching artificial intelligence to recognize specific species of microorganisms in cosmetic samples in terms of compliance with the ISO standard document, to develop of the Microorganism Detection System (SDM). (aaem.pl)
Stabilization of the arsenic redox species for as many as 15 months is demonstrated for samples that have been properly filtered and acidified with HCl in the field. (usgs.gov)
In order to overcome this, the biofilm growth in SSFs is modelled using a system of non-linear balance laws of convection-diffusion-reaction type with discontinuous flux for the concentration of species in the biofilm matrix and the flowing liquid suspension. (lu.se)

The assay was linear over the concentration range 0.50-500.00 ng/mL, with correlation coefficients ≥0.995. (unboundmedicine.com)
Colloid concentration and size distribution will be measured by photon correlation spectroscopy. (dtu.dk)
There was no correlation between lamotrigine concentrations and dosages neither for pediatric nor for adult groups. (bvs.br)

Preconcentration of a sample when trace anions (low µg/L) are quantified. (thermofisher.com)
Preconcentration requires extra hardware such as a concentration column and pump, and takes extra time. (thermofisher.com)

Sensitising potential of the test item was predicted from the mean peptide depletion of both analysed peptides (cysteine and lysine) by comparing the peptide concentration of the test item treated samples to the corresponding reference control. (europa.eu)
Make an experimental plan for preparing and analyzing protein and peptide samples using mass spectrometry. (lu.se)

An example that could improve your sample prep optimization would be to validate for total protein concentration or have options for protein purification through a tag affinity strategy. (ahdiagnostics.se)
Set up and perform experimental work including purification, concentration and separation of samples. (lu.se)

Rapid concentration and isotopic measurements of ultra-trace 235U fission products with comparison to an ORIGEN isotope deple. (ornl.gov)

Lemos, D. , Camargo, C. , Camargo, M. and Landgraf, M. (2019) Evaluation on the Concentration of Heavy Metals in Surface Waters in the Municipality of Pratápolis-MG. Open Access Library Journal , 6 , 1-13. (scirp.org)

FLU is almost completely absorbed after oral administration, due to first-pass hepatic metabolism, bioavailability is about 70%, with peak plasma concentration between 6 and 8 hours after administration and extensive tissue accumulation ( Catterson, Preskorn, 1996 Catterson ML, Preskorn SH. (scielo.br)

The concentration range is 50 to 1000 mg/1. (coastalwiki.org)

Subsequently, most of the target protein is removed through MWCO ultrafiltration, while the HCPs are enriched, reducing the concentration difference between HCPs and the target protein. (pharmiweb.com)

Drug therapy adherence was assessed by validated questionnaires [Brief Medication Questionnaire (BMQ) and Morisky-Green test (MG)] and by the blood concentration of fluoxetine and its active metabolite norfluoxetine. (scielo.br)

Results from clinical studies suggest that pregnancy alters hepatic drug metabolism in a cytochrome P450 (P450) isoform-specific manner, and rising concentrations of female hormones are potentially responsible for the changes. (aspetjournals.org)

Reverse osmosis (ionic separation) separates ions or molecules using a semipermeable membrane or barrier. (sigmaaldrich.com)
Salts, ionic molecules and ionic detergents interfere with isoelectric focusing and compromise protein separation. (thermofisher.com)

Consequently, there is a need to determine Pd concentrations in biological and environmental samples. (usp.br)
The peak retention time and area of the standard is compared to the test sample's results to determine the unknown concentration. (sigmaaldrich.com)
This article describes how different pump samplers can be used to determine the silt and sand concentration in a suspended load . (coastalwiki.org)
An optical sensor can be used to determine the silt concentration in the bottle after settling of the sand particles. (coastalwiki.org)
A siphon sampler was used to determine the actual concentration (co) at the position of the intake nozzle. (coastalwiki.org)
The method was applied successfully to determine nalbuphine concentrations in human plasma samples obtained from subjects receiving intravenous administration of nalbuphine. (unboundmedicine.com)
A chromatographic method was validated to determine lamotrigine concentrations. (bvs.br)

Samples can be collected from solid, liquid, or gaseous materials. (sigmaaldrich.com)
A solid-phase extraction-liquid chromatographic-tandem mass spectrometry method for the determination of nalbuphine concentrations in human plasma has been developed. (unboundmedicine.com)
Liquid hydrocarbons from the separation process are routed to production tanks (figure 2). (cdc.gov)

The MWCO-based HCP characterization technique involves the following steps: first, the sample is treated with surfactant to release the interaction between the target protein and HCPs. (pharmiweb.com)

Area and personal air samples were taken and analyzed for mercury and methyl-methacrylate (80626). (cdc.gov)
Mercury concentrations in the air ranged from 4.1 to 13 micrograms per cubic meter (microg/m3) for 22 personal samples and from 5.3 to 11microg/m3 in eight area samples. (cdc.gov)
Area air samples revealed mercury concentrations all below 20microg/m3. (cdc.gov)

For information on calculating appropriate amounts of local anesthetic/epinephrine, please refer to the Calculating Concentrations and Dilutions section below. (medscape.com)

The plasmatic concentration of fluoxetine and norfluoxetine indicated that 58.5% volunteers were within the recommended therapeutic range and thus considered adherent to drug therapy. (scielo.br)
Most fluoxetine users showed a plasma concentration of fluoxetine and norfluoxetine within the therapeutic range, despite the low adherence to the drug therapy evaluated by the questionnaires. (scielo.br)
1B in Figure 3 presents the average error in the concentration as a function of the discharge Q s showing an average error smaller than 20% for a discharge Q s in the range 0.2 to 1 1/min. (coastalwiki.org)
Arsenic(III) recoveries for samples spiked with aluminum, chromium(III), cobalt, iron(II), lead, manganese, nickel, selenium(VI), and zinc ranged from 84 to 107 percent over the entire concentration range tested. (usgs.gov)
1.5 was found in the tested concentration range. (europa.eu)

Kelley and her coauthors wrote that one important next step will be to test the method on earlier-stage cancer patients, whose samples typically exhibit much lower numbers of CTCs. (genomeweb.com)
Patients with stable potassium concentration 3 mEq/L ( 3 mmol/L) typically have a significant potassium deficit. (msdmanuals.com)

Contemporary cnidarian-algae symbioses are challenged by increasing CO 2 concentrations (ocean warming and acidification) affecting organisms' biological performance. (nature.com)

Engineering, Lund University in environmental samples are generally time consuming. (lu.se)

A rapid, sensitive method is described for the determination of 5-(methylamino)-2-phenyl-4-(3-(trifluromethyl)phenyl)-3-(2H)-furanone (RE-40885) concentrations in three soil types. (epa.gov)
The degree of interference for each metal without cation-exchange on the determination of total arsenic and arsenic(III) was evaluated by spiking synthetic samples containing arsenic(III) and arsenic(V) with the potential interfering metal. (usgs.gov)

This sample is filtered onto an IR-transparent substrate and measured in its entirety by FT-IR IMaging to capture all particles present on the filter. (bruker.com)
The objective of this project in to improve the understanding of element transport from the continets to the oceans by using radioisotpoes of Sr and Nd.Suspended particles, colloidal size fractions and truly dissolved material in oxic and anoxic brackish water, comprising a vertical profile in the central baltic Sea will be samples in 1997. (dtu.dk)

Examples of this include ICP-MS (Inductively-Coupled Plasma - Mass Spectrometry), where volatilization of a sample occurs in the first step, and measuring of the concentration occurs in the second. (newworldencyclopedia.org)
Plan and execute the best suited sample preparations and mass spectrometry acquisition. (lu.se)

The shake-flask method was performed as the base scenario to examine solubility differences by two off-line sampling separation techniques: (1) centrifuging or (2) filtering. (aiche.org)
2-DE is a robust method in which the resolution acquired during the first dimension separation is preserved during the second dimension. (thermofisher.com)
In a study published this week in Nature Chemistry , the researchers tested the method, called single-cell mRNA cytometry, first on cell lines, and then in a small number samples from patients with prostate cancer, hinting at potential clinical applications in oncology. (genomeweb.com)
A PCR method was utilized for initial screening of samples after an overnight enrichment in tryptic soy broth. (nih.gov)
In this method a known quantity of the element or compound under study is added, and the difference between the concentration added, and the concentration observed is the amount actually in the sample. (newworldencyclopedia.org)
Briefly, the method was consisted of a 4 mL venous blood epileptic seizures, primarily and secondarily generalized tonic-clonic sample collected in heparinized tube previously to the morning LTG seizures, absence seizures and the drop-attacks associated to Lennox- dose ingestion (trough level). (bvs.br)

The main challenge with the use of low-grade resources is the difficulty with the efficient and economical separation of phosphorus from the other constituent elements, such as iron, aluminum, and magnesium. (rsc.org)

The concentration of each component needs to be determined empirically and experimental conditions require optimization. (thermofisher.com)
our observations about the influence of polytherapies on lamotrigine pharmacokinetics confirm the relevance of quantifying this antiepileptic drug plasma concentrations in the process of treatment optimization. (bvs.br)

When preparing samples for GC, it's important to minimize sample complexity because the quality of the sample impacts the accuracy and precision of your final chromatographic results. (sigmaaldrich.com)

In this study, we develop an integrated process for the direct recovery and separation of dicalcium phosphate dihydrate for fertilizer and livestock feed additive production from a low-grade (2.0 wt% P) iron-rich (19.7 wt% Fe) phosphate ore. (rsc.org)

Then, to provide a hint at potential clinical utility, they picked two targets - TMPRSS2/ERG and AR-V7 - to search for in blood samples collected from a small cohort of patients who were undergoing treatment for metastatic castration-resistant prostate cancer. (genomeweb.com)

Consequently, higher levels of LMWH or UH in the sample lead to lower chromogenic intensity. (medscape.com)

The induction was mainly observed when the average hormone concentrations were at the levels reached during pregnancy, suggesting that these effects are likely pregnancy-specific. (aspetjournals.org)

In the MWCO-based technique, target protein samples containing HCPs are dried and dissolved in Tris HCl dissociation buffer containing surfactants. (pharmiweb.com)

The other samples yielded low-grade mineral concentrates with low platinum-group values, and/or the platinum-group metals did not concentrate with the primary mineral value. (cdc.gov)

A low level of anti-Xa may be seen if the specimen is not collected at the right time or if there was a delay in separation of the plasma from the cellular component of the blood. (medscape.com)

For each concentration the largest and smallest deviation are also indicated. (coastalwiki.org)

Polar samples always show good solubility in the aqueous mobile phase used in HILIC, which overcomes the drawbacks of the poor solubility often encountered in NP-LC. (springer.com)

It is built from methylated aromatic alcohols that are linked contributed to the total organic carbon in territorial samples by ether and carbon bonds. (lu.se)

Anatomy15

Organisms7

Diseases1

Chemicals and Drugs71

Analytical, Diagnostic and Therapeutic Techniques and Equipment83

Psychiatry and Psychology6

Phenomena and Processes60

Disciplines and Occupations11

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture18

Information Science7

Health Care7

Chromatographic separation5

Methods15

Analyte3

Compounds6

High19

HPLC6

Instrument's1

Filtration4

Quantification2

Chromatography4

Proteins4

Calibration2

Characterize3

Laboratory4

Reagents1

Significantly1

Bacteria3

Fluids2

Antibiotic1

Analysis13

Matrices1

Microbial1

Sediment2

Optimal1

Preparation6

Contamination3

Serum5

Exposure2

Species3

Correlation3

Preconcentration2

Peptide2

Purification2

Measurements1

Waters1

Tissue1

10001

Ultrafiltration1

Blood concentration1

Potentially1

Ionic2

Determine7

Liquid3

Characterization1

Mercury3

Refer1

Range5

Typically2

Organisms1

Environmental1

Determination2

Particles2

Mass2

Method6

Phosphorus1

Optimization2

Accuracy and preci1

Process1

Patients1

Higher1

Pregnancy1

Protein samples1

Concentrate1

Plasma1

Deviation1

Solubility1

Total1