Saline hepes buffer. Medical search. Frequent questions

In contrast, it can be used with phosphate-buffered saline or MOPS. (wikipedia.org)
Preparation of mind fractions Brains from adult Wistar rats (220 g) were rapidly eliminated and put into ice-cold phosphate-buffered saline (PBS). (cell-signaling-pathways.com)

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl 2 solution containing the DNA. (sigmaaldrich.com)
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl 2 is recommended.For preserving solutions stored at 4 ºC, 0.8% sodium azide can be used. (vectorlabs.com)

For this reason, DEPC cannot be used with Tris or HEPES buffers. (wikipedia.org)

Remove the supernatant and re-suspend the pellet in HEPES buffered saline to double the final desired cell concentration. (jove.com)
This buffer contains an optimal concentration of calcium that facilitates the binding of Annexin V to phosphatidylserine on the cell surface, which is essential for the detection of early-stage cell apoptosis by flow cytometry. (stemcell.com)
for incompatible buffers and low antibody concentrations, use our rapid antibody purification and concentration kits . (abcam.com)

Whole brains were homogenized in 20 mM HEPES (pH 7.4) containing 0.32 M sucrose 150 mM NaCl 1 mM MgCl2 0.5 mM CaCl2 and 20 mM NEM. (cell-signaling-pathways.com)

1 mg/mL in Hepes-Buffered Saline (HBS)-Buffered Saline (HBS) with 0.02% azide as a preservative. (mitosciences.com)

Remove cover slips from slides and place slides in a staining jar containing lysis buffer. (neb.com)

Terbium-labeled anti-STAT1 [pTyr701] antibody in HEPES buffered saline. (fishersci.com)

Prepare 1X enzyme reaction buffer . (neb.com)
Wash slides in a staining jar with enzyme buffer 3 times, 5 minutes each wash. (neb.com)
Place 50 µl of the enzyme solution or buffer alone onto gel surface and cover with a 22 x 22 mm cover slip. (neb.com)

If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected] . (abcam.com)

After many washes in extraction buffer proteins were eluted from the streptavidin beads by boiling in reducing sample buffer and then resolved by SDS-PAGE and immunoblotted using rabbit polyclonal antibodies against GluR1 (1/2 0 Upstate Biotechnology) and/or GluR6/7 (1/2 0 Upstate Biotechnology). (cell-signaling-pathways.com)

A chemical gradient was still detected at pH 7.4 in the usual HEPES-based transport buffer in contrast to what was observed in a bicarbonate/CO 2 -buffered medium. (aspetjournals.org)

This antifolate gradient correlated with an alkaline intracellular pH in the former (pH 7.85), but not the latter (pH 7.39), buffer and was abolished by the protonophore carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone. (aspetjournals.org)
The augmentation of intracellular pH, when cells are in a HEPES buffer, should be taken into consideration in studies that encompass all proton-coupled transporter families. (aspetjournals.org)
and the buffer exchanged to the intracellular remedy for patch pipette using NAP-5 Sephadex G-25 columns. (cell-signaling-pathways.com)

3. Dilute 1 mM Fura 2-AM DMSO solution with HEPES buffer saline to prepare 1 mM Fura 2-AM working solution. (dojindo.com)
According to the methods, a mixture of lipids containing a protonatable or deprotonatable lipid, for example an amino lipid and a lipid such as a PEG- or Polyamide oligomer-modified lipid is combined with a buffered aqueous solution of a charged therapeutic agent, for example polyanionic nucleic acids, to produce particles in which the therapeutic agent is encapsulated in a lipid vesicle. (justia.com)

From buffer solutions to cell culture media formulations at bench top or production sales, Caisson Labs gives you the products you need in a way that makes sense to you. (caissonlabs.com)
Hi, I'm planning to use the Cell-ID 20-Plex Pd Barcoding Kit, and I noticed that some of the buffer solutions (e.g. the 1X Barcode Perm Buffer) are prepared by dilution in PBS. (stanford.edu)

Saline-treated male mice with HSC-3 tumors exhibited less oral cancer-induced nociceptive behavior and had more β-endorphin protein in the cancer microenvironment than saline-treated female mice with HSC-3 tumors. (frontiersin.org)

So, something like HEPES would probably work, or TBS if you really want to keep the saline. (stanford.edu)

The remaining surface biotin was removed with GSH buffer (pH 9 2 min) then lysed and incubated with streptavidin beads to isolate internalized biotinylated proteins. (cell-signaling-pathways.com)