Saccharomyces interactions of membrane proteins. Medical search. Frequent questions

Here, we develop a highly parallel protein-protein interaction sequencing (PPiSeq) platform that uses a novel double barcoding system in conjunction with the dihydrofolate reductase protein-fragment complementation assay in Saccharomyces cerevisiae . (nature.com)
Many different protein fragment designs have been used with PCA, however the highest-throughput version uses a split murine dihydrofolate reductase (mDHFR) that, once reconstructed, allows colony growth on agar containing the S. cerevisiae DHFR inhibitor methotrexate (MTX). (nature.com)
Em Saccharomyces cerevisiae , Rrp43p é uma proteína que faz parte do exossomo, um complexo multiproteíco que atua no processamento de snoRNAs, snRNAs e rRNAs e na degradação de mRNA. (usp.br)
Utilizando Rrp43p como " isca" estudamos interações com outras proteínas expressas em Saccharomyces cerevisiae , na procura de proteínas envolvidas em alguns eventos de processamento de RNA, que pudessem ajudar a esclarecer em maior detalhe o papel do exossomo na célula. (usp.br)
In the yeast Saccharomyces cerevisiae , Rrp43p is one of the eleven subunits of the exosome, a complex involved in the processing of snoRNAs, snRNAs and rRNAs, and in mRNA degradation. (usp.br)
We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. (thebiogrid.org)
Igor Stagljar has begun a study into Yeast, looking into Saccharomyces cerevisiae and Two-hybrid screening. (research.com)
With his scientific publications, his incorporates both Saccharomyces cerevisiae and Yeast. (research.com)
Igor Stagljar is investigating Yeast as part of his inquiry into Saccharomyces cerevisiae and Two-hybrid screening. (research.com)
Igor Stagljar merges many fields, such as Saccharomyces cerevisiae and Yeast, in his writings. (research.com)
We have determined that the P6 protein interacts with a C2 calcium-dependent membrane-targeting protein (designated Arabidopsis [Arabidopsis thaliana] Soybean Response to Cold [AtSRC2.2]) in a yeast (Saccharomyces cerevisiae) two-hybrid screen and have confirmed this interaction through coimmunoprecipitation and colocalization assays in the CaMV host Nicotiana benthamiana. (unige.ch)
Purification of a RAS - responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method. (microbiologyresearch.org)
The pro-region of the Kex2 endoprotease of Saccharomyces cerevisiae is removed by self-processing. (eu.org)
Saccharomyces cerevisiae, Yarrowia lipolytica, and Lipomyces starkeyi . (biomedcentral.com)
Our results with E. coli , in conjunction with recent observations made with the yeast Saccharomyces cerevisiae , raise the possibility that the SRP receptor-mediated formation of intracellular membrane networks is governed by evolutionarily conserved principles. (rupress.org)
Liposomes were prepared from lipids extracted from L-α-phosphatidylcholine, Escherichia coli , yeast ( Saccharomyces cerevisiae ) and bovine liver cells through agitation and sonication. (insplorion.com)
Diploid cells of the yeast Saccharomyces cerevisiae form after the mating of two haploid cells of the opposite mating type. (rupress.org)
Mutants have been expressed in Saccharomyces cerevisiae and characterized: mutants S19D and S57D-and to a lesser extent also mutants S22D and S27D-are deregulated, as shown by their low activation by CaM and by tryptic cleavage of the N-terminus. (unimi.it)
Here, we illustrate a protocol to precipitate the complex formed by human epitope-tagged AFG3L2 and paraplegin heterologously expressed in the yeast Saccharomyces cerevisiae . (researchsquare.com)
van 't Klooster JS, Cheng TY, Sikkema HR, Jeucken A, Moody B, Poolman B. Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins. (avantilipids.com)
To understand the molecular biology of peptide hormone action, we studied response of budding yeast ( Saccharomyces cerevisiae ) to its peptide mating pheromones ( a -factor and α-factor). (berkeley.edu)
Most of the seminal studies on the regulatory mechanisms of autophagosome biogenesis were conducted on Saccharomyces cerevisiae or culture cells in vitro ( Hamasaki and Yoshimori, 2010 ). (frontiersin.org)
These proteins do not exist in the related, non-pathogenic baker´s yeast Saccharomyces cerevisiae, indicating that they could be related to pathogenicity. (fraunhofer.de)
PeroxisomeDB contains the complete peroxisomal proteome of Homo sapiens (encoded by 85 genes) and Saccharomyces cerevisiae (encoded by 61 genes). (216.92.14)
Tra1 is an essential 437-kDa component of the Saccharomyces cerevisiae SAGA/SLIK and NuA4 histone acetyltransferase complexes. (biomedcentral.com)
TRA1 is an essential gene in Saccharomyces cerevisiae that encodes a 437 kDa protein product. (biomedcentral.com)
Though the group did the mapping with ABC transporters from a yeast species - Saccharomyces cerevisiae - many of the proteins are homologous to disease-linked human ABC proteins. (genomeweb.com)
In yeast (Saccharomyces cerevisiae), complex III and complex IV are respiratory chain complexes capable of transferring electrons to oxygen converting it to water. (lu.se)

Mpc proteins are highly conserved from yeast to humans and are necessary for the uptake of pyruvate at the inner mitochondrial membrane, which is used for leucine and valine biosynthesis and as a fuel for respiration. (upv.es)
AFG3L2 and paraplegin are cognate ATP-dependent metalloproteases that constitute the m -AAA protease complex in the inner mitochondrial membrane. (researchsquare.com)
This process results in the creation of a proton gradient over the inner mitochondrial membrane, which drives the ATP synthesis. (lu.se)

The endoplasmic reticulum (ER) is the site of protein synthesis for secretory and membrane proteins. (uni-tuebingen.de)
The Sec61 complex forms a protein-conducting channel in the endoplasmic reticulum (ER) membrane that is required for secretion of soluble proteins and production of many membrane proteins. (biorxiv.org)
Secretory proteins exit the endoplasmic reticulum (ER) in coat protein complex II (COPII)-coated vesicles and then progress through the Golgi complex before delivery to their final destination. (biologists.com)
Many endoplasmic reticulum (ER) were transferred into a special "Chrysanthemum flower center" (CFC) in which several double-layer isolation membranes (IM) were formed and extended. (frontiersin.org)

These proteins take part in the fusion pathway in two consecutive steps. (centrodinoferrari.com)
During her post-doctoral training, she examined structure-function relationships for SEC14p, a yeast phospholipid transfer protein involved in the late stages of the secretory pathway. (bradley.edu)
Here we show that in cells depleted of the SRP protein, Ffh or the translocon component SecE, the ribosomal targeting pathway is blocked downstream and unprecedented, membrane-bound FtsY-ribosomal complexes are captured. (rupress.org)
This latter export pathway requires the membrane potential. (tcdb.org)
The chloroplast albino 3 (ALB3) protein appears to integrate the light harvesting chlorophyll-binding protein into thylakoid membranes using a pathway that is distinct from the chloroplast Sec translocation pathway. (tcdb.org)
In this Commentary, I review evidence in favor of the idea that partitioning of TMDs into bilayer domains that are endowed with distinct physico-chemical properties plays a pivotal role in the transport of membrane proteins within the early secretory pathway. (biologists.com)
The combination of such self-organizational phenomena with canonical intermolecular interactions is most likely to control the release of membrane proteins from the ER into the secretory pathway. (biologists.com)
We have recently shown that this same pathway modulates the content of integral membrane proteins and, in collaborative studies, that it is also involved in maintenance of the sterol composition of the plasma membrane. (berkeley.edu)
The aim of PEROXISOME database ( PeroxisomeDB ) is to gather, organise and integrate curated information on peroxisomal genes, their encoded proteins, their molecular function and metabolic pathway they belong to, and their related disorders. (216.92.14)
The classical pathway is triggered by interaction of the Fc portion of an antibody (immunoglobulin [Ig] M, IgG1, IgG2, IgG3) or C-reactive protein with C1q. (medscape.com)
Lectins activate the lectin pathway in a manner similar to the antibody interaction with complement in the classical pathway. (medscape.com)
for example, the proteins factor H and factor I inhibit the formation of the enzyme C3 convertase of the alternative pathway. (medscape.com)

Recently, it has been recognized that membrane-bound ribosomes are crucial for biogenesis of integral membrane proteins in E. coli , thus renewing interest in ribosome targeting to and association with the membrane in this organism. (rupress.org)

His Receptor Protein-Tyrosine Kinases research extends to the thematically linked field of Receptor tyrosine kinase. (research.com)
Receptor Protein-Tyrosine Kinases is closely attributed to Tyrosine kinase in his research. (research.com)
The Wee1 class of protein-tyrosine kinase has an important role in cell cycle control. (berkeley.edu)
We investigated control mechanisms that regulate the activity, localization, and stability of Wee1, especially the bud neck-localized protein kinase Hsl1 and its more distant paralogs (Gin4 and Kcc4), in particular their recruitment to septin filaments, which assemble at the presumptive site of cell division. (berkeley.edu)
Activation of the pheromone receptor-coupled G protein initiates a four-tiered cascade of protein kinases, ultimately resulting in stimulation of a messenger-activated protein kinase or MAPK (Fus3) that translocates into the nucleus. (berkeley.edu)
Dr. Franz-Josef Johannes detected a novel protein kinase termed PKCµ. (fraunhofer.de)
In addition, calcofluor white sensitivity of the strain was enhanced by the protein kinase inhibitor staurosporine, a phenotype shared with the Ada components of the SAGA/SLIK complex. (biomedcentral.com)

Its genome, which is approximately 14.1 million base pairs, is estimated to contain 4,970 protein-coding genes and at least 450 non-coding RNAs. (wikipedia.org)
This comprehensive network maps genetic interactions for essential gene pairs, highlighting essential genes as densely connected hubs. (thebiogrid.org)
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. (thebiogrid.org)
EN] Mot3 and Rox1 are transcriptional repressors of hypoxic genes. (upv.es)
Disruption of the genes encoding other Sec63p-associated proteins (Sec71p and Sec72p) also results in karyogamy defects. (rupress.org)
However, we now have a number of methods that allow identification of genes critical for survival in a host as well as methods that allow direct measurement of gene expression during interaction with a host. (cdc.gov)
The interacting genes are involved in a range of cellular processes including gene expression, mitochondrial function, and membrane sorting/protein trafficking. (biomedcentral.com)
This analysis did not identify any synthetic lethal interactions but did reveal 23 synthetic slow growth interactions, many in combination with deletions of genes involved in cell membrane/wall processes. (biomedcentral.com)
This analysis identified 114 genes displaying synthetic sick/lethal (SSL) interactions with tra1 SRR 3413 . (biomedcentral.com)
Genes involved in transcription, RNA processing, mitochondrial function and membrane sorting/protein trafficking were prevalent. (biomedcentral.com)
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)
Genes that encode the proteins of complement components or their isotypes are distributed throughout different chromosomes, with 19 genes comprising 3 significant complement gene clusters in the human genome. (medscape.com)

In eukaryotes, secretory proteins are first translocated into the ER by the Sec61 complex before reaching the cell surface by vesicular trafficking. (biorxiv.org)

The biogenesis of most proteins in the mitochondrial matrix and the inner membrane relies on the function of the TIM23 complex. (frontiersin.org)
The present study was designed to investigate in vivo subcellular evidence for the biogenesis of autophagosomal membrane during spermiogenesis using transmission-electron microscopy (TEM), Western blots and immunohistochemistry in samples from the Chinese soft-shelled turtle. (frontiersin.org)
It is unclear if and where distinct membrane sources fuse during autophagosome biogenesis. (frontiersin.org)
In the present study, a subcellular mechanism for autophagosomal membrane biogenesis was examined in detail during in vivo turtle spermiogenesis. (frontiersin.org)

Initially, the dimerization of mitofusins results in the tethering of the outer membranes of adjoining mitochondria. (centrodinoferrari.com)
Mitochondria import nuclearly-encoded proteins, made in the cell cytoplasm, into the mitochondrial matrix where their mitochondrial targeting sequences are removed by proteolysis. (tcdb.org)
2001 ). Mitochondria have two, one for co-translational, and one for post-translational insertion of membrane proteins. (tcdb.org)
Mitochondria inherited three inner membrane translocases Sec, TAT and Oxa1 (YidC) from its bacterial ancestor. (tcdb.org)

New technologies for purifying membrane-bound protein complexes in combination with cryo-electron microscopy (EM) have recently allowed the exploration of such complexes under near-native conditions. (researchgate.net)
The accumulation of FtsY-ribosome complexes induces the formation of intracellular membranes needed for their quantitative accommodation. (rupress.org)
The protocol can be adapted to the analysis of other protein complexes. (researchsquare.com)
Vesicles from Golgi complexes, plasma membranes and endosomes might also be the sources of the autophagosome membrane. (frontiersin.org)
It won't work if you want to co-immunoprecipitate them or apply any proteomic biochemical method because you will have to use detergents, and then you destroy all the protein complexes. (genomeweb.com)

S. pombe can degrade L-malic acid, one of the dominant organic acids in wine, which makes them diverse among other Saccharomyces strains. (wikipedia.org)
Observing how the protein interactome changes across perturbations would require re-assaying all ∼ 18 million strains for each perturbation studied. (nature.com)
Strains were transformed with nudE variants in pAid vector and grown at 43°C. Numbers refer to amino acid residues of NUDE protein expressed by the constructs (see Fig. 3 for detailed amino acid sequences). (xenbase.org)
The phenotypes and genetic interactions of these strains also point to a role for Tra1 in the cellular response to stress. (biomedcentral.com)
Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein. (yeastgenome.org)

The cell membrane is mainly composed of lipid bilayers with inserted proteins and carbohydrates. (insplorion.com)
Lipid bilayers made of purified or synthetic lipids are widely used for estimating the effect of target compounds on cell membranes. (insplorion.com)

Atg18/WIPIs belong to the PROPPIN family of proteins. (nature.com)
In yeast, a member of the WASP family of proteins promotes the assembly of actin filaments around the site where endocytosis will occur. (elifesciences.org)

This basic two-step process has been confirmed in most mammalian cells, although its regulation and the repertoire of specific accessory proteins are likely to be highly context dependent. (centrodinoferrari.com)
They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)
Actin is one of the most abundant proteins in yeast, mammalian and other eukaryotic cells. (elifesciences.org)
These proteins are needed for many other processes in yeast and other cells, including mammalian cells. (elifesciences.org)

Usually only superficial infections of mucous membranes do occur. (fraunhofer.de)
Superficial infections of skin and mucous membranes are the most common types of cutaneous candidiasis. (medscape.com)

Previous in vivo studies indicated that unlike the E. coli signal recognition particle (SRP), the SRP receptor FtsY is required for membrane targeting of ribosomes. (rupress.org)
A second group of methods, which includes DNA microarrays and proteomics, have advantages that overcome the limitations implicit in signature-tagged mutagenesis and in vivo expression technology, namely, the ability to directly measure expression (gene or protein) levels on a true genome-wide scale, but their application to analysis of bacterial pathogens during real infections is still in its infancy. (cdc.gov)
Briefly, MYTH adapts a previously developed split ubiquitin method as an in vivo sensor of protein-protein interactions. (genomeweb.com)

We study the proteins transporting compounds and signals across the cell membranes. (cas.cz)

SNARE proteins are essential components of the machinery that facilitates membrane fusion in eukaryotic cells. (gla.ac.uk)
The smaller proteins are generally of prokaryotic origin while the larger ones are of eukaryotic origin. (tcdb.org)
The larger eukaryotic and archaeal proteins possess N- and C-terminal hydrophilic extensions. (tcdb.org)
Peroxisomes are single-membrane subcellular organelles, present in most eukaryotic cells and organisms. (216.92.14)

The most relevant proteins involved in the mitochondrial fusion process are three GTPase dynamin-like proteins: mitofusin 1 (MFN1) and 2 (MFN2), located in the outer mitochondrial membrane, and optic atrophy protein 1 (OPA1), in the inner membrane. (centrodinoferrari.com)
Fusion of the outer mitochondrial membrane depends on two GTPase family members: mitofusin 1 (MFN1) and mitofusin 2 (MFN2). (centrodinoferrari.com)
Before fusion, curving of the outer membranes is promoted by the phospholipase D-dependent hydrolysis of cardiolipin. (centrodinoferrari.com)
Kar2p is localized to the luminal (i.e., noncytoplasmic) face of the ER membrane, yet nuclear fusion must initiate from the cytosolic side of the outer nuclear membrane or the ER membrane with which it is contiguous. (rupress.org)
The coat on the budding vesicle comprises two layers, an inner layer of adaptor proteins (gray ovals) and an outer layer that forms a polyhedral cage. (biologists.com)

The prohormone-processing yeast KEX2 protease can act as an intracellular membrane protein or a soluble, secreted endopeptidase. (eu.org)

Aiming to a better understanding of the Rrp43p subunit function on RNA processing, we started a screening for Rrp43p-interacting proteins through the yeast two hybrid system. (usp.br)
NUDF protein interacts with the Aspergillus NUDE coiled-coil in a yeast two-hybrid system, while human LIS1 interacts with the human homologue of the NUDE/RO11 coiled-coil and also the Xenopus MP43 coiled-coil. (xenbase.org)
Stagljar and his colleagues previously developed a method, called a membrane yeast two-hybrid, or MYTH assay to allow them to study interactions of full-length membrane proteins in their natural cellular environment. (genomeweb.com)

It mediates fusion of synaptic vesicles with the presynaptic plasma membrane resulting in exocytosis of neurotransmitters. (researchgate.net)
The plasma membrane of the spermatid invaginated to form vesicles that were distributed among various endosomes around the CFC during spermiogenesis. (frontiersin.org)

Microscopy of internal structures of Sendai virus associated with the cytoplasmic surface of host membranes. (microbiologyresearch.org)
2022 ). The YidC transmembrane (TM) groove is essential for a high- affinity interaction, and the hydrophilic nature of the YidC groove plays an important role in protein transport across the cytoplasmic membrane bilayer to the periplasmic side. (tcdb.org)
V-ATPase is regulated by a unique mechanism that involves reversible dissociation into V₁-ATPase and V₀ proton channel, a process that involves breaking of protein interactions mediated by subunit C, the cytoplasmic domain of subunit "a" and three "peripheral stalks," each made of a heterodimer of E and G subunits. (rcsb.org)

The yeast SM protein Vps45p regulates membrane fusion through the trans-Golgi / late endosomal system, and interacts directly with the syntaxin (Tlg2p) and the v-SNARE (Snc2p) proteins. (gla.ac.uk)
There is both genetic and biochemical evidence that Kar2p interacts with Sec63p, an ER membrane protein containing both luminal and cytosolic domains that is involved in protein translocation across the membrane. (rupress.org)
The LIS1 -related NUDF protein of Aspergillus nidulans interacts with the coiled-coil domain of the NUDE/RO11 protein. (xenbase.org)
To achieve this, WASP interacts with several other proteins including WIP and myosin, a motor protein that moves along actin filaments to generate mechanical forces. (elifesciences.org)

In 2006, sub-cellular localization of almost all the proteins in S. pombe was published using green fluorescent protein as a molecular tag. (wikipedia.org)
A global genetic interaction network maps a wiring diagram of cellular function. (thebiogrid.org)
Her recent research efforts developed out of a collaboration with Dr. William A. Dunn and focus on the role of the Vac8 protein in a cellular recycling process known as autophagy using the yeast Pichia pastoris as the model system. (bradley.edu)
Autophagy is the main cellular process responsible for degrading defective organelles and long-lived proteins. (frontiersin.org)
One half of a split ubiquitin molecule is attached to an ABC transporter (or another cellular protein of interest) and the other half to its potential interaction targets. (genomeweb.com)
Actin-related protein 2/3 (Arp2/3) complex activation by nucleation promoting factors (NPFs) such as WASP, plays an important role in many actin-mediated cellular processes. (elifesciences.org)
The MAC exerts powerful killing activity by creating perforations in cellular membranes. (medscape.com)

Patrick JW, Laganowsky A. Probing Heterogeneous Lipid Interactions with Membrane Proteins Using Mass Spectrometry. (avantilipids.com)
No mass spectrometry results found for this protein. (yeastrc.org)

The universally conserved heterotrimeric Sec61 complex (SecY in prokaryotes) plays essential roles in biosynthesis of more than one third of proteins in all species (for review, see ref. 1 - 4 ). (biorxiv.org)
Davydova NY, Dangi B, Maldonado MA, Vavilov NE, Zgoda VG, Davydov DR. Toward a systems approach to cytochrome P450 ensemble: interactions of CYP2E1 with other P450 species and their impact on CYP1A2. (avantilipids.com)
Researchers from the University of Toronto have created a map of the protein interactions of 19 members of the ATP-binding cassette transporter class in a species of yeast, combining this data with previously-reported ABC transporter interactions into a comprehensive "interactome. (genomeweb.com)

In Escherichia coli , ribosomes must interact with translocons on the membrane for the proper integration of newly synthesized membrane proteins, cotranslationally. (rupress.org)

2018 ). Through YidC, proteins are inserted into the lipid bilayer via the SecYEG-dependent complex, but YidC functions as a chaperone in protein folding processes. (tcdb.org)

The concept of synthetic lethality has gained popularity as a rational guide for predicting chemotherapeutic targets based on negative genetic interactions between tumor-specific somatic mutations and a second-site target gene. (thebiogrid.org)
The study of Gene is intertwined with the study of Interaction network in a number of ways. (research.com)
Interaction network is closely attributed to Gene in his study. (research.com)
The P6 protein of Cauliflower mosaic virus (CaMV) is responsible for the formation of inclusion bodies (IBs), which are the sites for viral gene expression, replication, and virion assembly. (unige.ch)
Analysis of the Sendai virus M gene and protein. (microbiologyresearch.org)
NUDF protein, the product of the nudF gene, displays 42% sequence identity with the human protein LIS1 required for neuronal migration. (xenbase.org)
The product of the nudE gene isolated in the screen, NUDE, is a homologue of the nuclear distribution protein RO11 of Neurospora crassa. (xenbase.org)

2020) by determination of changes in plasma-membrane potential and other methods. (cas.cz)

A similar coiled-coil domain is present in several putative human proteins and in the mitotic phosphoprotein 43 ( MP43 ) of X. laevis. (xenbase.org)

All the analysed mutations affect the kinetics of interaction with CaM to some extent: in most cases, the altered kinetics result in marginal changes in affinity, with the exception of mutants S57D (K-D similar to 10-fold higher than wild-type ACA8) and S99D (K-D about half that of wild-type ACA8). (unimi.it)

At different stages of the insertion process, conformational changes in YidC's TM domain and membrane core have a mechanistic effect on Pf3 coat protein insertion. (tcdb.org)

A hierarchal cluster analysis revealed that the pattern of SSL interactions for tra1 SRR 3413 most closely resembles deletions of a group of regulatory GTPases required for membrane sorting/protein trafficking. (biomedcentral.com)
Deficiency of any of these regulatory proteins results in a state of overactivation of the complement system, with damaging inflammatory effects. (medscape.com)

The Sec1p / Munc18 (SM) family are a conserved group of proteins that regulate membrane fusion through interactions with their cognate syntaxins. (gla.ac.uk)
Mgr2p/Romo1 bridges TIM23 to the presequence translocase-associated motor (PAM) and is thought to regulate the lateral release of proteins harboring stop-transfer sequences. (frontiersin.org)

Graded scoring techniques that assign a fitness to each colony have been employed elsewhere, for example, in synthetic genetic interaction screens 16 . (nature.com)
To identify genetic interactions for TRA1 and provide insight into its function we have performed a systematic genetic array analysis (SGA) on tra1 SRR 3413 , an allele that is defective in transcriptional regulation. (biomedcentral.com)
We have demonstrated a genetic association of Tra1 with nuclear, mitochondrial and membrane processes. (biomedcentral.com)
addressed this question by studying yeast cells with genetic mutations affecting one or more of these proteins. (elifesciences.org)

With purified protein and soluble substrates, the exoglucanase activity of the GH7 domain embedded in the Fusion 3 construct in L. starkeyi was significantly higher than that of the GH7 domain in TeTr CBHI expressed alone. (biomedcentral.com)

The yeast extracted lipid membranes had the strongest response to introduction of choline laurate while the bovine liver lipid extracted liposomes were the most sensitive towards the shorter choline carboxylates. (insplorion.com)

Alterations in mitochondrial dynamics due to mutations in proteins involved in the fusion-fission machinery represent an important pathogenic mechanism of human diseases. (centrodinoferrari.com)

Membrane-bound ribosomes in E. coli were extensively studied over 20 years ago. (rupress.org)
The gating of this protein-selective pore has been extensively studied in patch clamp experiments on isolated mitochondrial membranes. (frontiersin.org)

For the membrane fusion step, one required component, the ER luminal protein Kar2p (BiP), has been identified. (rupress.org)
Taken together, these results suggest a direct role for Sec63p, Sec71p, and Sec72p in nuclear membrane fusion and argue against the alternative interpretation that the karyogamy defects result as an indirect consequence of the impaired membrane translocation of another component(s) required for the process. (rupress.org)

For example, actin filaments are needed to pull part of the membrane surrounding the cell inwards to bring molecules from the external environment into the cell by a process called endocytosis. (elifesciences.org)
The experiments show that WASP, myosin and WIP are recruited to sites where endocytosis is about to occur through specific interactions with other proteins. (elifesciences.org)
suggest that WASP and WIP play key roles in establishing the network of proteins needed for actin filaments to assemble during endocytosis. (elifesciences.org)

During this time, she worked with Dr. William A. Dunn, Jr. on the structure-function relationship of Vac8p, a vacuolar protein involved in the membrane altering steps of regulated autophagy in yeast. (bradley.edu)
Specifically, her lab group has been identifying structural motifs in the Vac8 protein that are important for membrane association, protein-protein interactions, and vesicle fusion events occurring during the autophagy of peroxisomes in response to glucose adaptation. (bradley.edu)
The testis expressed LC3-II protein, which was located within spermatids at different stages of differentiation and indicated active autophagy. (frontiersin.org)

In this study we expected to find proteins interacting with Rrp43p, which were involved in some aspects of RNA processing, and would improve the current knowledge on the exosome function. (usp.br)
We also found the interaction of Rrp43p with a protein of yet uncharacterized function (here named 137p). (usp.br)
This interaction, identified in the two hybrid system, was also confirmed through co-immunoprecipitation analysis, and the study of 137p function might bring new insights on Rrp43p function and control. (usp.br)
Formulation of unifying hypotheses describing how SM proteins function has been problematic, primarily due to the multiple modes of interaction that have been characterised for different members of this family binding to their cognate SNARE proteins. (gla.ac.uk)
they exert their function mainly through their interaction to the Qa-SNAREs (Syntaxins). (uni-goettingen.de)
Understanding the structure-function relationships for membrane-associated proteins involved in protein-trafficking events is Dr. Fry's primary research interest. (bradley.edu)
We propose that an ER/nuclear membrane protein complex composed of Sec63p, Sec71p, and Sec72p plays a central role in mediating nuclear membrane fusion and requires ER luminally associated Kar2p for its function. (rupress.org)
We also studied the roles of other classes of protein kinases (Cla4) and additional post-translational modifications (SUMOylation) in septin complex assembly, formation of different septin-based supramolecular ensembles, disassembly of septin-containing structures, and the function of septin organization in the events required for cell division and membrane septation during cytokinesis. (berkeley.edu)
The highly conserved NH(2)-terminal coiled-coil domain of the NUDE protein suffices for protein function when overexpressed. (xenbase.org)
The second largest, with 16 percent, marks interactions with proteins of unknown function. (genomeweb.com)

This process depends on the general physico-chemical features of the cargo membrane protein and on the interactions of these features with the collective properties of the bilayer, instead of the one-to-one intermolecular interactions that exist between discrete signals and their receptors. (biologists.com)

The SGA analysis revealed 114 synthetic slow growth/lethal (SSL) interactions for tra1 SRR 3413 . (biomedcentral.com)
As the lack of synthetic lethal interactions may have arisen from an incomplete selection against diploids in the automated screens, we repeated the screen in a strain background that selects more strongly against diploids. (biomedcentral.com)

Ypk1, in turn, regulates (via its phosphorylation of multiple substrates) maintenance of sphingolipid and glycerolipid homeostasis and bilayer lipid organization in the plasma membrane. (berkeley.edu)

The latter, Stagljar said, is an important area for drug development, because it opens the possibility that you could block ABC transporter activity by blocking associated zinc transport proteins. (genomeweb.com)

Furin (PACE, paired basic amino acid cleaving enzyme, membrane associated receptor protein) is serine endoprotease responsible for processing a variety of substrates (proparathyroid hormone, transforming growth factor beta 1 precursor, proalbumin, pro-beta-secretase, membrane type-1 matrix metalloproteinase, beta subunit of pro-nerve growth factor and von Willebrand factor). (eu.org)
The important components of this system are various cell membrane-associated proteins such as complement receptor 1 (CR1), complement receptor 2 (CR2), and decay accelerating factor (DAF). (medscape.com)

In addition, the channel has a seam (lateral gate) in the wall that can open laterally in the plane of the membrane to release transmembrane segments (TMs) of membrane protein clients into the lipid phase. (biorxiv.org)
Some animal proteins, for example, those in the LAT family (TC# 2.A.3.8) including ASUR4 (gbY12716) and SPRM1 (gbL25068) associate with a type 1 transmembrane glycoprotein that is essential for insertion or activity of the permease and forms a disulfide bridge with it. (tcdb.org)
For membrane proteins, a third mechanism, based on the interaction of their transmembrane domain (TMD) with lipid microdomains, must also be considered. (biologists.com)

Bacteria also export (to the periplasm) N-tails of membrane proteins synthesized without leader sequences by a Sec (Type IIPS)-independent mechanism. (tcdb.org)
This dual sorting mechanism is currently being investigated, and in yeast appears to be regulated by a recently discovered subunit, the Mgr2 protein. (frontiersin.org)

Insertion of a membrane protein via the Oxa1p export machinery follows the 'positive-inside' rule for membrane protein topology. (tcdb.org)

EN] The plasma membrane contains a wide spectrum of transporter proteins, each responsible for a facet of the cell's nutritional and signalling requirements. (upv.es)
In the group's ABC transporter study, Stagljar and his team used MYTH to map the interactions of all of S. cerevisiae's 19 non-mitochondrial ABC transporters. (genomeweb.com)
They then combined the MYTH screening results with previously reported ABC transporter physical interactions available through the BioGRID database. (genomeweb.com)
The group went on in its recent study to investigate two potentially interesting associations revealed in the yeast ABC transporter interactome map: physical interactions of ABC transporters with one another and the interaction of these molecules with members of the zinc transport system. (genomeweb.com)

An AtSRC2.2 protein fused to red fluorescent protein (RFP) was localized to the plasma membrane and specifically associated with plasmodesmata. (unige.ch)
After fusion of the two plasma membranes of the mating cells, a dinucleated cell forms initially in which the two haploid nuclei then rapidly fuse to form a single diploid nucleus. (rupress.org)
ACA8 is a plasma membrane-localized isoform of calmodulin (CaM)-regulated Ca2+-ATPase of Arabidopsis thaliana. (unimi.it)
Phosphorylation of serine residues in the N-terminus modulates the activity of ACA8, a plasma membrane Ca2+-ATPase of Arabidopsis thaliana / S. Giacometti, C.A. Marrano, M.C. Bonza, L. Luoni, M. Limonta, M.I. De Michelis. (unimi.it)
We have shown that Ypk1, a member of the AGC class of protein kinases conserved from yeast to humans, is the essential target of and activated via phosphorylation by the plasma membrane-associated TORC2 complex. (berkeley.edu)

Igor Stagljar, the study's senior author, told ProteoMonitor this week that his team has been interested broadly in how integral membrane proteins interact at the genome-wide level. (genomeweb.com)

For ASH1 E3 LE recognition by She2p/She3p, the she2p tetramer uses two identical sets of amino acids (N36, R43, R52, K60, and R63) to contact RNA, while its protruding helix seems to be important for interaction with She3p. (uni-tuebingen.de)
PACE4 (paired basic amino acid cleaving system 4, SPC4) is a calcium-dependent serine endoprotease that can cleave precursor protein at their paired basic amino acid processing sites. (eu.org)
This result and other findings suggested that Mgr2p controls the lateral release of inner membrane proteins harboring a stop-transfer signal that follows an N-terminal amino acid signal. (frontiersin.org)

The structures indicate that Sec61 inhibitors stabilize the plug domain of Sec61 in a closed state, thereby preventing the protein-translocation pore from opening. (biorxiv.org)
sec61 and sec62 mutant alleles that cause similar or more severe protein translocation defects show no karyogamy defects. (rupress.org)
The TIM23 complex is a hub for translocation of preproteins into or across the mitochondrial inner membrane. (frontiersin.org)
Deletion of Mgr2p has been found to delay protein translocation into the matrix and accumulation in the inner membrane. (frontiersin.org)
The core of the TIM23 complex contains a pore that is essential for protein insertion into or translocation across the inner membrane. (frontiersin.org)

SNARE proteins are subject to multiple levels of regulation, one of which is imbedded in the syntaxin (Qa-SNARE) molecule. (gla.ac.uk)

Anatomy38

Organisms15

Chemicals and Drugs96

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Phenomena and Processes78

Technology, Industry, Agriculture5

Information Science4

Health Care2

Cerevisiae28

Inner mitochon3

Endoplasmic4

Pathway12

Organism1

Kinase7

Genes12

Secretory1

Biogenesis4

Mitochondria4

Complexes5

Strains5

Lipid bilayers2

Family of proteins2

Mammalian4

Mucous membranes2

Vivo3

Cell membranes1

Eukaryotic4

Outer5

Intracellular membrane1

Yeast two-hy3

Vesicles2

Cytoplasmic3

Interacts4

Cellular7

Mass Spectrometry2

Species3

Escherichia1

20181

Gene7

20201

Putative1

Mutants1

Conformational1

Regulatory2

Regulate2

Genetic4

Soluble1

Liposomes1

Mutations1

Extensively studied2

Component2

Endocytosis3

Autophagy3

Function10

Physico-chemical1

Synthetic2

Bilayer1

Transport proteins1

Receptor2

Transmembrane3

Mechanism2

Topology1

Transporter4

Plasma5

Genome-wide1

Amino3

Translocation5

Regulation1