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HexosesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSaccharomycesGenes, FungalFungal ProteinsGene Expression Regulation, FungalMolecular Sequence DataMutationAmino Acid SequenceBase SequenceRNA, FungalHexosephosphatesGlucoseGenome, FungalChromosomes, FungalCloning, Molecularbeta-FructofuranosidasePlasmidsGenetic Complementation TestMonosaccharide Transport ProteinsSpores, FungalSequence Homology, Amino AcidGalactoseGene DeletionKineticsTranscription, GeneticPhenotypeFermentationPentosesSuppression, GeneticBiological TransportMethylglucosidesDNA-Binding ProteinsRestriction MappingTranscription FactorsXyloseHaploidyVacuoles3-O-MethylglucoseTemperatureGenes, SuppressorMutagenesisDeoxy SugarsRecombination, GeneticTransformation, GeneticFructoseDeoxyglucoseDiploidySequence AlignmentSilent Information Regulator Proteins, Saccharomyces cerevisiaeRecombinant Fusion ProteinsEscherichia coliMitochondriaCell Cycle ProteinsMembrane ProteinsSpheroplastsMethylglycosidesPentose Phosphate PathwayProtein BindingSubstrate SpecificityCell WallSchizosaccharomycesAllelesCulture MediaGenes, Mating Type, FungalHexokinaseCarrier ProteinsRNA, MessengerSaccharomycetalesRecombinant ProteinsMeiosisDNA PrimersGlucose Transporter Type 5DNA RepairGlycolysisNuclear ProteinsKluyveromycesGlucosephosphatesMaltoseBinding SitesCarbohydrate MetabolismAdenosine TriphosphatasesMembrane Transport ProteinsGlycoside HydrolasesRad52 DNA Repair and Recombination ProteinEthanolSpecies SpecificityGlucose Transport Proteins, FacilitativeCell CycleRepressor ProteinsBiological Transport, ActivePichiaGenesModels, BiologicalPromoter Regions, GeneticMannoseErgosterolKiller Factors, YeastTrehalose
SugarsStrainsYeastsTransporterFermentationEthanolBioethanol productionBaker's yeastPentosesSpeciesEthanologenic yeastRecombinantGenesUptakeCarbohydratesProteins2001Metabolic networkGrowthGeneticRegulationDiffusionCellsCellGlucoseTransportersActive Hexose CHXT5StrainRegulationS288CProteinInhibitionReproducesPathwayProductionFaster
Sugars9
  • In this study, the facilitated diffusion transport system for hexose sugars of S. cerevisiae was replaced by hexose-proton symport. (biomedcentral.com)
  • Lignocellulosic biomass, rich in hexose and pentose sugars, is an attractive resource for commercially viable bioethanol production. (vtt.fi)
  • Saccharomyces cerevisiae and Pachysolen tannophilus , were used for fermentation of hexose and pentose sugars in the hydrolysate. (ncsu.edu)
  • High ethanol yields are dependent on co-fermentation of hexose and pentose sugars present in lignocellulosic hydrolysates. (pku.edu.cn)
  • Carbon is obtained mostly from hexose sugars, such as glucose and fructose, or disaccharides such as sucrose and maltose. (lutheransatcmu.org)
  • Efficient fermentation processes to produce ethanol from both the hexose and pentose sugars available in low-cost lignocellulosic biomass are sought to support the expansion of the biofuels industry. (cmich.edu)
  • During the last years we have successfully developed technologies (" C5-technology ") to engineer yeast ( Saccharomyces cerevisiae ) strains for the fermentation of pentose sugars like xylose and arabinose. (goethe-university-frankfurt.de)
  • They use hexose sugars as … Fermentation occurs in the absence of oxygen (anaerobic conditions), and in the presence of beneficial microorganisms (yeasts, molds, and bacteria) that obtain their energy through fermentation. (zestrestaurant.co.za)
  • Kim J.-H., Block D.E., Mills D.A. (2010) Simultaneous consumption of pentose and hexose sugars: an optimal microbial phenotype for efficient fermentation of lignocellulosic biomass, Appl. (ifpenergiesnouvelles.fr)
Strains4
  • Saccharomyces strains were used widely and traditionally for industrial ethanol production because of its ability to produce high concentrations of ethanol from hexoses and its high tolerance to ethanol and other inhibitory compounds. (scialert.net)
  • The inherently higher robustness and tolerance of S. cerevisiae to various inhibitors gives it a head start in programs aimed at developing strains with extreme inhibitor tolerance, able to efficiently ferment hexoses and pentoses in concentrated lignocellulose hydrolysates [ 6 ]. (biomedcentral.com)
  • Here, we tested ten non-conventional yeasts for bread fermentation, including two Saccharomyces species that are not currently used in bread making and 8 non-Saccharomyces strains. (omicsdi.org)
  • Grotkjœr T., Christakopoulos P., Nielsen J., Olsson L. (2005) Comparative metabolic network analysis of two xylose fermenting recombinant Saccharomyces cerevisiae strains, Metabolic Eng. (ifpenergiesnouvelles.fr)
Yeasts5
  • The promoter includes DNA with promoter activity of high osmolarity response 7 gene (HOR7 gene), glycelaldehyde 3 phosphate dehydrogenase 2 gene (TDH2 gene), heat shock protein 30 gene (HSP30), hexose transport protein 7 gene (HXT7 gene), thioredoxin peroxidase 1 gene (AHP1 gene), or membrane protein 1 associated gene gene) of yeasts. (justia.com)
  • With the use of this promoter, recombinants transformed to produce organic acids such as lactic acid in yeasts, including Saccharomyces , can be obtained by genetic engineering, and such recombinants are useful as those that have high productivity of organic acids. (justia.com)
  • The term yeast is often taken as a synonym for Saccharomyces cerevisiae,[8] but the phylogenetic diversity of yeasts is shown by their placement in two separate phyla: the Ascomycota and the Basidiomycota. (lutheransatcmu.org)
  • The antibiotic cycloheximide is sometimes added to yeast growth media to inhibit the growth of Saccharomyces yeasts and select for wild/indigenous yeast species. (lutheransatcmu.org)
  • The resulting so-called hexose transporter ( hxt ) null strain (EBY.VW4000) has become a valuable tool worldwide for the characterization of sugar transporters from other yeasts, fungi, plants, animals and even human cells (click here to see how the tool works). (goethe-university-frankfurt.de)
Transporter6
  • GLUT proteins transport glucose and related hexoses according to a model of alternate conformation, which predicts that the transporter exposes a single substrate binding site toward either the outside or the inside of the cell. (wikipedia.org)
  • DNA sequence analysis carried out in this study has characterised the two remaining genes as HXT4=LGT1, a low affinity hexose transporter and a new thiamine regulated gene, THI5. (le.ac.uk)
  • The S. cerevisiae has 20 genes that encode hexose transporter proteins. (ac.ir)
  • lt;br />Discussion and conclusion: The deduced protein sequence showed a high similarity to Hxt6p VL31(EGA76254.1) sequences registered in NCBI and also the highest similarity of this gene with HXT7 ( one of the hexose transporter) was observed. (ac.ir)
  • Monitoring Mig1 migration in cells expressing different glucose uptake systems indicated that the profile of Snf1-Mig1 activity parallels the characteristics of the expressed hexose transporter, suggesting a firm link between glucose uptake and the regulation of the SNF1 pathway. (avhandlingar.se)
  • Transcript levels of the sucrose transporter NtSUT1 and hexose transporter NtMST1 encoding genes increased significantly in the source leaves and roots of Vac-Inv lines, whereas increased NtMst1 transcript levels were also detected in the roots of Apo-Inv lines. (sun.ac.za)
Fermentation3
Ethanol6
  • In anaerobic cultures, a higher ethanol yield can be achieved when transport of hexoses is proton-coupled, because of the lower net ATP yield of sugar dissimilation. (biomedcentral.com)
  • To apply this strategy to increase the ethanol yield on sucrose, we constructed a platform strain in which all genes encoding hexose transporters, disaccharide transporters and disaccharide hydrolases were deleted, after which a combination of a glucose-proton symporter, fructose-proton symporter and extracellular invertase ( SUC2 ) were introduced. (biomedcentral.com)
  • After evolution, the resulting strain exhibited a 16.6% increased anaerobic ethanol yield (from 1.51 to 1.76 mol mol hexose equivalent −1 ) and 46.6% decreased biomass yield on sucrose. (biomedcentral.com)
  • This study provides a proof-of-concept for the replacement of the endogenous hexose transporters of S. cerevisiae by hexose-proton symport, and the concomitant decrease in ATP yield, to greatly improve the anaerobic yield of ethanol on sugar. (biomedcentral.com)
  • Saccharomyces is a potentially useful organism for the commercial production of ethanol as it is not capable of fermenting starch containing in the mango peel. (scialert.net)
  • Introduction: The Saccharomyces cerevisiae yeast is one of the most important microorganisms to produce ethanol. (ac.ir)
Bioethanol production1
Baker's yeast1
  • This thesis describes the characterisation of a set of six genes of the baker's yeast Saccharomyces cerevisiae, that are induced when a molasses-grown culture reaches the end of rapid fermentative growth. (le.ac.uk)
Pentoses3
Species1
Ethanologenic yeast1
Recombinant1
  • Research Advances on Xylose-Fermenting Recombinant Saccharomyces cerevisiae[J]. Acta Scientiarum Naturalium Universitatis Pekinensis. (pku.edu.cn)
Genes1
Uptake2
  • In such environments, the high extracellular glucose concentration can drive the uptake of glucose by yeast cells via diffusion, which is exemplified by the occurrence of many hexose transporters with a facilitated diffusion mechanism in S. cerevisiae . (biomedcentral.com)
  • Transformation was assessed by the standard parameters of morphological alteration, increased hexose uptake, loss of density inhibition, and anchorage-independent growth. (embl.de)
Carbohydrates1
Proteins1
20011
  • Expression of Photosynthetic Gene psaC of Cyanobacteria in Saccharomyces cerevisiae [J]. Acta Scientiarum Naturalium Universitatis Pekinensis, 2001, 37(6): 755-759. (pku.edu.cn)
Metabolic network1
Growth1
  • their products enable the cell to utilise alternative hexoses as growth substrates. (le.ac.uk)
Genetic1
  • We generated a global genetic interaction network for Saccharomyces cerevisiae, constructing more than 23 million double mutants, identifying about 550,000 negative and about 350,000 positive genetic interactions. (thebiogrid.org)
Regulation1
Diffusion1
Cells1
  • For example, Leucosporidium frigidum grows at -2 to 20 °C (28 to 68 °F), Saccharomyces telluris at 5 to 35 °C (41 to 95 °F), and Candida slooffi at 28 to 45 °C (82 to 113 °F).[18] The cells can survive freezing under certain conditions, with viability decreasing over time. (lutheransatcmu.org)
Cell2
Glucose14
Transporters3
  • The genes for hexose transporters HXT11 and HXT9, and asparagine-utilization are absent in all Sb strains. (nih.gov)
  • Because the expression of HXT5 is regulated differently than that of the major hexose transporters, the metabolism of degradation of Hxt5p was investigated. (uu.nl)
  • The localization at the vesicular structures and the vacuole suggested that the degradation of Hxt5p is similar to that of the major hexose transporters. (uu.nl)
Active Hexose C1
  • AHCC* (Active Hexose Correlated Compound) is a special extract from Shiitake mushroom (Lentinula edodes), which is well known as a powerful antioxidant that aggressively combats free radicals and modulates the immune system. (naturalhealthgarden.ca)
HXT51
  • The hexose transporter Hxt5 is involved in synthesis of trehalose. (uu.nl)
Strain1
Regulation2
  • Solari, C.A. Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae. (uba.ar)
  • Portela, P. 'Regulation of PKA activity by an autophosphorylation mechanism in Saccharomyces cerevisiae' (2014) Biochemical Journal. (uba.ar)
S288C1
  • Saccharomyces cerevisiae S288C 14-3-3 family protein BMH2 (BMH2), partial mRNA. (genscript.com)
Protein1
  • Therefore, as an alternative, CaMdr1p and Cdr1p, which is an ABC protein (ATP binding cassette) also involved in azole resistance in C. albicans , were independently expressed in a common hypersensitive host JG436 of Saccharomyces cerevisiae . (ias.ac.in)
Inhibition2
  • The GRC probably works via inhibition of a hexose kinase, Hxk2p, which is inhibited by trehalose-6-phosphate. (uu.nl)
  • glycolysis, which is the inhibition of the HK module by hexose monophosphate. (nih.gov)
Reproduces1
  • A yeast is a unicellular fungus which reproduces asexually by budding or division, especially the genus Saccharomyces which is important in food fermentations (Walker, 1988). (fao.org)
Pathway1
  • 17. Non-oxidative synthesis of pentose 5-phosphate from hexose 6-phosphate and triose phosphate by the L-type pentose pathway. (nih.gov)
Production1
  • In the tropics, Saccharomyces pombe is the dominant yeast in the production of traditional fermented beverages, especially those derived from maize and millet (Adams and Moss, 1995). (fao.org)
Faster1
  • Overexpression of either gene resulted in faster hexose catabolism, but no cumulative effect was observed with the simultaneous overexpression. (unimib.it)