• The recent abundance of genome sequence data has brought an urgent need for systematic proteomics to decipher the encoded protein networks that dictate cellular function. (visualcomplexity.com)
  • I reveal that Saccharomyces cerevisiae Rtt109p promotes genome stability and resistance to DNA-damaging agents, and that it does this by functionally cooperating with the histone chaperone Asf1p to maintain normal chromatin structure. (bl.uk)
  • The single dynein motor encoded by the S. cerevisiae genome performs an important but nonessential spindle-positioning role. (rupress.org)
  • Given that the contribution of Sec14p-like proteins to the PITP complement of mammalian cells is completely uninvestigated, and that the mammalian genome encodes many proteins of this class, we anticipate such advances will directly and positively impact our understanding of the molecular basis of such diseases. (uab.edu)
  • RRM3 and its encoded protein have been shown to be vital for cellular replication, specifically associating with replication forks genome-wide. (wikipedia.org)
  • The proteins' G-quadruplex ability has been shown to reduce G4-related genome damage when there is low cellular levels of Pif1. (wikipedia.org)
  • The binding of these two proteins appears to be linked to inappropriate replication timing and genome integrity. (wikipedia.org)
  • Multiple 2-plex datasets can be combined after separate analyses, but there is a high likelihood that different sets of peptides and proteins will be identified between each experiment. (mcponline.org)
  • We have developed a multiplexed set of reagents for quantitative protein analysis that place isobaric mass labels at the N termini and lysine side chains of peptides in a digest mixture. (mcponline.org)
  • Absolute quantitation of targeted proteins can also be achieved using synthetic peptides tagged with one of the members of the multiplex reagent set. (mcponline.org)
  • In the first step, the proteins identified by a single protein identification tool were classified into two groups: high confidence proteins that were identified by unique peptides, and low confidence proteins that were identified by non-unique peptides. (openthesis.org)
  • The proteins that were identified by the presence of unique peptides and that were commonly identified by different tools were grouped into the highest confidence level - Level 4. (openthesis.org)
  • According to the operation of tandem mass spectrometry and the characteristics of the peptides generated by site-specific protease digestion, a two-pass approach for identifying the species-specific proteins was developed. (openthesis.org)
  • The methods described here should be applicable to the hydrodynamic analysis and subsequent purification of any soluble protein from organisms amenable to genetic manipulation, such as yeast, as long as the function of that protein is not perturbed by the addition of an epitope tag. (springer.com)
  • The exploitation of the yeast Saccharomyces cerevisiae as a biological model for the investigation of complex molecular processes conserved in multicellular organisms, such as humans, has allowed fundamental biological discoveries. (mdpi.com)
  • RESULTS: At a cut off at 20% identity over protein length, all three organisms share 1738 proteins, whereas both fungi share only 541 conserved proteins. (biomedsearch.com)
  • Results of this study suggest that easily available raw materials such as fruit peels offer cost-effective substrates for production of commercially important microbial proteins for alarming global issues linked to protein malnutrition.Conflict of interest: The authors declare no conflict of interest. (magiran.com)
  • Saccharomyces cerevisiae is a robust microbial platform widely used in biofuel production, based on its amenability to systems and synthetic biology tools. (osti.gov)
  • The vital challenges for the efficient microbial conversion of lignocellulosic biomass by engineered S. cerevisiae include heterologous expression of cellulolytic enzymes, co-fermentation of hexose and pentose sugars, and robustness against various stresses. (osti.gov)
  • Semiquantitative biochemical analysis of the corresponding mutant proteins revealed that each of the 21 amino acid alterations caused simultaneous defects in every single protein-protein interaction and in Gal80's structural integrity. (genetics.org)
  • The ICAT quantitative labeling strategy ( 3 , 4 ) is perhaps the best-characterized method for relative protein quantitation using MS. Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine ( 5 ), lysine ( 6 ), tyrosine ( 7 ), and leucine ( 8 ), for in vivo incorporation of a mass difference to support relative quantitation. (mcponline.org)
  • When comparing yeast and human proteins, it is clear that both amino acid sequences and protein functions are often very well conserved. (mdpi.com)
  • Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. (oregonstate.edu)
  • It's about 40kDa, localized to the cytosol and represents about 1% to the total protein (by Coomasie staining by eye) when grown in minimal glucose media. (bio.net)
  • In this paper it is reported that the Cdc25 protein, in addition to its stimulatory role in the RAS/adenylate cyclase pathway, regulates glucose transport. (nih.gov)
  • Because the Cdc25 protein is localized at the membrane, these results indicate that Cdc25 is directly involved in glucose transport and may be in direct contact with the glucose transporters. (nih.gov)
  • Unlike most postexponential proteins, p35 was not regulated by heat shock or glucose repression. (asm.org)
  • Well in advance of glucose exhaustion, a transition phase was observed, characterized by a decrease in the growth rate and a progressive reduction of protein and RNA accumulation. (asm.org)
  • The occurrence of these new proteins in stationary-phase cells is presumed to result from the release of carbon catabolite repression due to glucose exhaustion. (asm.org)
  • We transformed wild-type S. cerevisiae with an S. cerevisiae URA3 -based GAL1 cDNA library and selected transformants in glucose synthetic complete plates lacking uracil (glucose SC minus uracil plates). (asm.org)
  • We show that prolonged glucose deprivation also leads to Snf1-dependent accumulation of Reg2 and that this protein helps to inhibit Snf1 and to accelerate growth recovery upon glucose replenishment. (asm.org)
  • The developed two-pass approach and two-step strategy were then used to study the protein profiling of Saccharomyces cerevisiae cultivated in various gravity conditions (10 and 300 g glucose/l) in order to investigate the changes in central metabolic pathways of S. cerevisiae. (openthesis.org)
  • The protein profile showed that a higher flux of nutrient was channelled into the pentose phosphate pathway when S. cerevisiae was grown under a high glucose concentration. (openthesis.org)
  • Here, we report a study of the meiotic phenotypes conferred by two missense mutations affecting the largest subunit of RPA, which are localized in the protein interaction domain ( rfa1-t11 ) and in the DNA-binding domain ( rfa1-t48 ). (genetics.org)
  • Zaki N, Lazarova Molnar S, El Hajj W, Campbell P. Protein-protein interaction based on pairwise similarity. (labome.org)
  • Zaki N, Efimov D, Berengueres J. Protein complex detection using interaction reliability assessment and weighted clustering coefficient. (labome.org)
  • In my thesis project, I set out to test this cooperativity hypothesis by examining the interaction of Sir proteins with well-defined in vitro reconstituted mono- and di-nucleosomes. (harvard.edu)
  • In a search for further proteins interacting with Ccz1p, we identified the endosomal soluble N-ethylmaleimide-sensitive factor attachment protein receptor Pep12p as an interaction partner of Ccz1p. (uni-bielefeld.de)
  • The interaction of these two nuclear proteins is enhanced in the presence of salts, an indication that the interaction between the two is likely hydrophobic. (wikipedia.org)
  • The interaction between PCNA and Rrm3p suggests that Rrm3p may be an accessory DNA helicase that helps replication fork progression through formation of a protein-protein complex with PCNA. (wikipedia.org)
  • In particular, as discussed here, septin-based structures recruit, and thereby localize (and, in some cases, regulate the activity of) a multiplicity of protein kinases that integrate multiple inputs into signaling pathways and ultimately initiate ensuing biological responses (Figure 1 ). (frontiersin.org)
  • Quantitative measures of protein dynamics in vivo are therefore highly useful for elucidating specific regulatory pathways. (g3journal.org)
  • A collection of articles that focus on an array of different scientific topics such as pathways, cancer, transmembrane proteins. (cusabio.com)
  • Two polypeptides of molecular weights 55,000 and 53,000, identified as α and β tubulin, and a polypeptide of molecular weight 63,000, associated with the nuclear DNA to a very high degree, account for nearly 50% of the nonhistone proteins present in chromatin. (springer.com)
  • An understanding of how different cargo proteins are segregated in the trans -Golgi and how extracellular signals control vesicle function is actively being sought, but the underlying molecular mechanisms remain poorly understood. (rupress.org)
  • The coenzym A-synthesizing protein complex (CoA-SPC) is a multienzyme complex of Saccharomyces cerevisiae (Bakers' yeast), which has a molecular weight in excess of 200,000 as determined by Sephadex G-200 column chromatography. (semanticscholar.org)
  • While the P. pastoris system has a much smaller set of vectors available and a less-well-established molecular biology, it can be cultured to very high densities [ 24 ] potentially producing large quantities of the protein of interest. (biomedcentral.com)
  • Analysis of the non-essential mutants in the regulatory subunits of Glc7 revealed that the lack of Reg1, and no other subunit, causes hypersensitivity to unfolded protein response (UPR)-inducers, which was concomitant with an augmented UPR element-dependent transcriptional response. (sigmaaldrich.com)
  • Living cells contain regulatory proteins that are involved in maintaining the redox status of their respective molecules. (asm.org)
  • The data (all of which are available online) will help future studies on individual proteins as well as functional genomics and systems biology. (harvard.edu)