Hadwiger J, Wittenberg C, Mendenhall M, Reed S (1989) The saccharomyces cerevisiae cks1 gene, a homolog of the schizo-saccharomyces pombe suc1+ gene, encodes a subunit of the cdc28 protein kinase complex. (edu.pl)
As a start to designing those gene-targeting tools, Elledge, now a professor of genetics at Harvard Medical School, began by trying to clone the mammalian homolog of recA , a bacterial gene required for DNA repair via recombination. (the-scientist.com)
Because there was no mammalian recA homolog, Elledge attempted to clone the Saccharomyces cerevisiae (baker's yeast) homolog using a novel method that included an antibody step. (the-scientist.com)
Phosphorylation3
The inhibition of Torc1 by rapamycin mimics nutrient starvation and causes an array of physiological changes that include global changes in gene expression, phosphorylation/dephosphorylation of various factors, decreased cell growth, degradation of proteins, and ultimately cell cycle arrest in the G 1 phase [4, 9-11]. (researchsquare.com)
Focusing on LKB1, heat shock protein (HSP) 90, and cell division cycle (CDC) 37 proteins, the protein expression, phosphorylation, and interaction were examined with western blot, immunofluorescence staining, and/or immunoprecipitation. (bvsalud.org)
The role of phosphorylation and the CDC28 protein kinase in cell cycle-regulated nuclear import of the S. cerevisiae transcription factor SWI5. (wikidata.org)
Cyclin4
In our previous research, we showed that the cyclin-dependent kinase regulatory subunit (CKS2) in maize (Zea mays L.) was induced by water deficit and cold stress. (edu.pl)
The clam embryo protein cyclin A induces entry into M phase and the resumption of meiosis in Xenopus oocytes. (wikidata.org)
Pathway6
These genes are part of the mating machinery in yeast but not necessary part of the mating pheromone response pathway. (openwetware.org)
The photoperiod-dependent pathway of floral promotion converges on the gene CONSTANS ( CO ), which is known to be expressed in a circadian manner [ 10 ]. (biomedcentral.com)
In yeast, rapamycin inhibits the TORC1 kinase signaling pathway causing rapid alteration in gene expression and ultimately cell cycle arrest in G 1 through mechanisms that are not fully understood. (researchsquare.com)
We show that the H2B R95A causes defects in the expression of a subset of genes of the pheromone pathway required for α factor-induced G 1 arrest. (researchsquare.com)
The expression of the STE5 gene and its encoded scaffold protein Ste5, required for the sequential activation of the MAPKs of the pheromone pathway, is greatly reduced in the H2B R95A mutant. (researchsquare.com)
Our data provide a new insight whereby rapamycin signaling via the Torc1 kinase may exploit the pheromone pathway to arrest cells in the G 1 phase. (researchsquare.com)
Yeast5
The authors have previously reported that the Salmonella typhimurium SigD protein, a phosphatidylinositol phosphatase involved in invasion of the host cell, inhibits yeast growth, presumably by depleting an essential pool of phosphatidylinositol 4,5- bis phosphate, and also that a catalytically inactive version, SigD R468A , was able to arrest growth by a different mechanism that involved disruption of the actin cytoskeleton. (microbiologyresearch.org)
And third, SigD caused strong activation of the yeast MAP kinase Slt2, whereas SigD R468A rather inactivated another MAP kinase, Kss1. (microbiologyresearch.org)
It is concluded that the Salmonella SigD protein deprived of its phosphatase activity is able to disrupt yeast morphogenesis by interfering with Cdc42 function, opening the possibility that the SigD N-terminal region might directly modulate small GTPases from the host during infection. (microbiologyresearch.org)
Nim1-related kinases coordinate cell cycle progression with the organization of the peripheral cytoskeleton in yeast. (microbiologyresearch.org)
Elledge had found that Rnr2 protein levels increased when yeast cells were grown in the presence of agents that damaged DNA. (the-scientist.com)
Inhibition1
In Saccharomyces cerevisiae , rapamycin binds to the peptidyl-prolyl isomerase Fpr1 leading to the inhibition of the Target of Rapamycin (TOR) kinase that forms the TOR Complex 1 (Torc1) [4, 5]. (researchsquare.com)
MAPK1
For example, under low-nitrogen conditions, crosstalk is observed between two signaling pathways in Saccharomyces cerevisiae , the cAMP and MAPK pathways, which are both downstream of the small GTPase Ras. (biomedcentral.com)
Regulatory3
Studying RNR2' s regulatory elements, he found those that were necessary to induce the production of higher protein levels in response to DNA damage and identified factors that bind these DNA elements to mediate the response of RNR2 to DNA damage. (the-scientist.com)
Furthermore, this work shows how membrane receptor guanylyl cyclases hijack the regulatory mechanisms used for active kinases to facilitate their regulation. (bvsalud.org)
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis. (wikidata.org)
Although a number of approaches have been used to predict gene functions and interactions, tools that analyze the essential coordination of functional components in cellular processes still need to be developed. (biomedcentral.com)
In this work, we present a new approach to study the cooperation of functional modules (sets of functionally related genes) in a specific cellular process. (biomedcentral.com)
A cooperative module pair is defined as two modules that significantly cooperate with certain functional genes in a cellular process. (biomedcentral.com)
Deletion1
A series of PZmCKS2-deletion derivatives, P0-P3, from the translation start code (-1,455, -999, -367, and -3 bp) was fused to the β-glucuronidase (GUS) reporter gene, and each deletion construct was analyzed by Agrobacterium-mediated steady transformation into Arabidopsis. (edu.pl)
Membrane1
As a membrane protein and non-kinase client of Hsp90-Cdc37, this work shows the remarkable plasticity of Cdc37 to interact with a broad array of clients with significant sequence variation. (bvsalud.org)
Actin1
These attachment sites are linked to actin-bundles that surround the nucleus via an Ndj1-Mps3-Csm4 protein bridge that spans the inner and outer nuclear membranes. (biorxiv.org)
Nuclear1
A beta-hairpin comprising the nuclear localization sequence sustains the self-associated states of nucleosome assembly protein 1. (colorado.edu)
Regulation2
He mentioned this to David Stillman , who was at Stanford to interview for a faculty position, and who studied cell cycle regulation of proteins as a postdoc in Kim Nasmyth 's lab at the MRC Laboratory of Molecular Biology in the U.K. Stillman pointed out that ribonucleotide reductase was cell cycle regulated-rather than remaining stable, the RNA and protein levels fluctuate throughout the cell cycle. (the-scientist.com)
At least 60 genes have been described as participants in flowering regulation [ 9 ]. (biomedcentral.com)
Analyzed gene expression1
We analyzed gene expression during Arabidopsis thaliana meristem development under long day conditions from day 7 to 16 after germination in one-day increments. (biomedcentral.com)
Transition2
We discovered an unprecedented burst of differential expression of cell cycle related genes at one particular point during transition to flowering. (biomedcentral.com)
Analysis of mutants with abnormal flowering time has allowed the identification of genes controlling floral transition [ 8 ]. (biomedcentral.com)
This method identifies cooperative module pairs that significantly influence a cellular process and the correlated genes and interactions that are essential to that process. (biomedcentral.com)
Upon exposure2
He found that RNR2 RNA levels increased dramatically, even more than the protein levels, upon exposure of cells to DNA damage and that mutations in RNR2 resulted in hypersensitivity to DNA damage. (the-scientist.com)
From these combined studies, it seems logical that the global gene expression pattern that ensues upon exposure to rapamycin must be governed by changes at the level of the chromatin structure. (researchsquare.com)
Expression4
Despite extensive studies of specific genes that regulate this process, the global changes in transcript expression profiles at the point when a vegetative meristem transitions into an inflorescence have not been reported. (biomedcentral.com)
This analysis revealed a developmental time point between 10 and 12 days after germination where FLC expression had decreased but LFY expression had not yet increased, which was characterized by a peak in the number of differentially expressed genes. (biomedcentral.com)
We have previously shown that the peptidyl-prolyl isomerase Rrd1 is required to mediate cellular response to rapamycin by altering gene expression [6, 7, 19, 20]. (researchsquare.com)
Here, we hypothesized that specific histone residue(s) might be involved in facilitating gene expression such that cells mount a response to rapamycin. (researchsquare.com)
Specific1
W hen he began a postdoc in Ronald Davis 's laboratory at Stanford University in 1984, Stephen Elledge wanted to develop new ways to knock out and mutate specific genes in mammals. (the-scientist.com)
Role1
To investigate the role of the liver kinase (LK) B1 protein, an activator of AMP-activated protein kinase (AMPK), in AMPK signaling suppression when exposed to vesicant, a kind of chemical warfare agent. (bvsalud.org)
Sequence1
Giuliano G, Pichersky E, Malik V, Timko M, Scolnik P, Cashmore A (1988) An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene. (edu.pl)
Phase1
Coupling of mitosis to the completion of S phase in Xenopus occurs via modulation of the tyrosine kinase that phosphorylates p34cdc2. (wikidata.org)
Human3
CDK3+protein,+human at the U.S. National Library of Medicine Medical Subject Headings (MeSH) CDK3 human gene location in the UCSC Genome Browser. (wikipedia.org)
CDK3 human gene details in the UCSC Genome Browser. (wikipedia.org)
Cloning and subcellular localization of a human phosphatidylinositol 3-phosphate 5-kinase, PIKfyve/Fab1. (microbiologyresearch.org)
Cells1
Modulation of chloride secretory responses and barrier function of intestinal epithelial cells by the Salmonella effector protein SigD. (microbiologyresearch.org)
After flowering initiation, the action of LFY and AP1 , as well as CAULIFLOWER ( CAL ) results in the upregulation of genes that control floral organ identity [ 20 - 23 ]. (biomedcentral.com)