• Huang G, Shigesada K, Ito K, Wee HJ, Yokomizo T, Ito Y. Dimerization with PEBP2beta protects RUNX1/AML1 from ubiquitin-proteasome-mediated degradation. (medlineplus.gov)
  • Runt-related transcription factor 1 (RUNX1) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. (wikipedia.org)
  • Runt-related transcription factor 1 gene ( RUNX1 ), also known as acute myeloid leukaemia 1 protein (AML1), plays a crucial role in the pathogenesis of AML. (mdpi.com)
  • RUNX1/AML1 is one of the most frequently mutated leukaemias associated with a poor prognosis in AML. (mdpi.com)
  • The protein, called AML1, plays a critical role in the development of the blood system and in the production of platelets and immune cells. (mskcc.org)
  • According to the study, investigators identified the methyltransferase enzyme that controls the activity of the normal AML1 protein - also called RUNX1 - demonstrating its ability to regulate the function of transcription factors, proteins that control cell fate by turning genes on or off. (mskcc.org)
  • The researchers found that the cellular pathways that regulate the activity of the normal AML1 protein through a process called arginine methylation cannot similarly regulate the activity of AML1-ETO, a protein associated with causing acute leukemia. (mskcc.org)
  • As a member of the core-binding factor family of transcription factors, RUNX1, also known as acute myeloid leukemia 1 protein (AML1), modulates cell proliferation, differentiation, and survival. (lipexogen.com)
  • Recently, we reported that the fusion protein AML1/MDS1/EVI1 (AME), a product of a t(3;21)(q26;q22) associated with chronic myelogenous leukemia and acute myelogenous leukemia, displays a complex pattern of self-interaction. (lincoln.ac.uk)
  • Germline mutations in the gene AML1 ( RUNX1, CBFA2 ) occur in the familial platelet disorder with predisposition for AML, an autosomal dominant disorder characterized by moderate thrombocytopenia, a defect in platelet function, and propensity to develop AML. (medscape.com)
  • AML1/RUNX1 gene point mutations in childhood myeloid malignancies. (cdc.gov)
  • RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. (wikipedia.org)
  • RUNX3 protein is a transcription factor, containing a highly conserved DNA binding domain which binds to a DNA core motif of 5′ pyGpyGGT 3′ designated as a "runt domain, RD" which shares a sequence similarity with D. melanogaster RUNX. (hindawi.com)
  • High-quality, lentiviral transcription factor (TF) reporter system that provides a sensitive fluorescent or luminescent readout for human/mouse RUNX family transcription factor 1 (RUNX1) transcriptional activity in mammalian cells. (lipexogen.com)
  • RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. (wikipedia.org)
  • In this study, we show that increased expression of Runt related transcription factor 1 (RUNX1) maintains undifferentiated state of CSC-like SP cells through upregulation of inhibitors of DNA binding/differentiation genes (ID1 and ID3) in late cisplatin-paclitaxel resistant cells. (techscience.com)
  • The protein-protein interaction network analysis of 397 upregulated genes in RUNX1-high samples of TCGA data show significant enrichment of pathways known to negatively regulate CSC differentiation. (techscience.com)
  • Intriguingly RUNX1 inhibition not only induces CSC differentiation but also downregulates anti-apoptotic protein BCL2 in both SP and NSP cells and potentiates cytotoxic effects of Cisplatin-Paclitaxel in chemoresistant EOC cells. (techscience.com)
  • Altogether, our data reveal new regulatory roles by RUNX1 to modulate CSC differentiation via ID1 and ID3 and to promote chemoresistance through BCL2 upregulation. (techscience.com)
  • Our scientists have developed a wide array of stem cell-focused reagents and resources for many applications including flow cytometry , western blotting , ELISAs , and recombinant proteins for cell differentiation. (biolegend.com)
  • RUNX1 is also required for the differentiation of CD8+, Th17, and regulatory T cells. (biolegend.com)
  • Impaired differentiation of megakaryocytes and decreased circulating platelet count were observed in the absence of RUNX1. (biolegend.com)
  • These findings revealed that RUNX1 acts as a tumor suppressor for myeloid leukemia and is crucial for the development and terminal differentiation of several blood cell lineages 2,3 . (biolegend.com)
  • To evaluate the leukemogenic role of RUNX1-MT in ASXL1 -mutated cells, we co-expressed RUNX1 -MT (R135T) and ASXL1 -MT (R693X) in different cell lines and performed immunoblot, co-immunoprecipitation, gene expression microarray, quantitative RT-PCR, cell proliferation, differentiation, and clonogenic assays for in vitro functional analyses. (biomedcentral.com)
  • The expression of RUNX1 mutant in ASXL1 -mutated myeloid cells augmented proliferation, blocked differentiation, and increased self-renewal activity. (biomedcentral.com)
  • RUNX1‑RUNX1T1 suppression supported myeloid differentiation by the differential expression of CEBPA, CEBPE, ID2, JMJD6, IKZF1, CBFB, KIT and CDK6, while MAPK1 depletion inhibited the differentiation of t(8;21) cells by elevated expression of ADA and downregulation of JUN. (prinsesmaximacentrum.nl)
  • We found that the hematopoietic master regulator RUNX1 controls the expression of certain microRNAs, of importance during erythroid/megakaryocytic differentiation. (uni-frankfurt.de)
  • Thus RUNX1/ETO contributes to increased expression of miR451 target genes and interferes with normal gene expression during differentiation. (uni-frankfurt.de)
  • RUNX1 thus emerges as a key regulator of a microRNA network, driving differentiation at the megakaryocytic/erythroid branching point. (uni-frankfurt.de)
  • We found that in vitro, this interaction alters the ability of RUNX1 to bind to DNA and to regulate a reporter gene, whereas in vivo, the expression of the isolated 8th zinc finger motif of EVI1 is sufficient to block the granulocyte colony-stimulating factor-induced differentiation of 32Dcl3 cells, leading to cell death. (lincoln.ac.uk)
  • Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. (cusabio.com)
  • Translocations and other types of mutations involving the RUNX1 gene have been associated with different types of leukemia and related blood disorders, including acute lymphoblastic leukemia (ALL), chronic myelomonocytic leukemia (CMML), familial platelet disorder with predisposition to acute myeloid leukemia, and myelodysplastic syndromes (MDS). (medlineplus.gov)
  • Acute myeloid leukemia in a child with familial platelet disorder and a cryptic runx1 intragenic deletion. (atlasgeneticsoncology.org)
  • Beyond Pathogenic RUNX1 Germline Variants: The Spectrum of Somatic Alterations in RUNX1-Familial Platelet Disorder with Predisposition to Hematologic Malignancies. (atlasgeneticsoncology.org)
  • The runt domain of RUNX1 binds to the core consensus sequence TGTGGNNN (where NNN can represent either TTT or TCA). (wikipedia.org)
  • RUNX1 can bind DNA as a monomer, but its DNA binding affinity is enhanced by 10 fold if it heterodimerises with the core binding factor β (CBFβ), also via the runt domain. (wikipedia.org)
  • We then found that VGLUT3-persistent neurons express the runt domain transcription factor Runx1. (jneurosci.org)
  • CRISPR Activation Screening Identifies VGLL3-TEAD1-RUNX1/3 as a Transcriptional Complex for PD-L1 Expression. (atlasgeneticsoncology.org)
  • Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
  • The RUNX1 reporter is constructed with tandem repeats of consensus RUNX1 DNA binding elements to read out its transcriptional activity. (lipexogen.com)
  • The reporter lentivirus is purified by PEG precipitation and sucrose gradient centrifugation, and is ideal for studying RUNX1 transcriptional activity in difficult-to-transfect cells including primary and/or thawed cells. (lipexogen.com)
  • Monitor transcriptional activity of RUNX1 in mammalian cells, screen for activators or inhibitors of RUNX1 transcriptional activity. (lipexogen.com)
  • Tumor suppressor genes encode proteins that normally provide negative control of cell proliferation. (medscape.com)
  • PTEN encodes a protein kinase of the same name and functions as a tumor suppressor through regulation of cell proliferation. (medscape.com)
  • BeadChip microarray and gene ontology analysis revealed that RUNX1‑RUNX1T1 and MAPK1 suppression reduced the proliferation rate of the t(8;21) cells with deregulated expression of several classical positive regulator genes that are otherwise known to enhance cell proliferation. (prinsesmaximacentrum.nl)
  • Therefore, we hypothesized that there may be a protein that acts as a tumor inhibitor during these early stages of life, preventing the development of leukemia. (lu.se)
  • RUNX1 and RUNX1-ETO: roles in hematopoiesis and leukemogenesis. (medlineplus.gov)
  • The RNA-binding protein IGF2BP3 is critical for MLL-AF4-mediated leukemogenesis. (uclahealth.org)
  • To date, most of our understanding of epigenetic regulation and its participation in leukemogenesis is based on the enzymatic activities and protein-protein interactions of histone and DNA modifiers. (frontiersin.org)
  • Furthermore, the leukemogenic RUNX1/ETO fusion protein transcriptionally represses the miR144/451 pre-microRNA. (uni-frankfurt.de)
  • This mini-review focusses on the role of RUNX1 mutations as the most common secondary leukemogenic hit in MDS/AML evolving from severe congenital neutropenia (SCN). (molcells.org)
  • These domains are necessary for RUNX1 to mediate DNA binding and protein-protein interactions respectively. (wikipedia.org)
  • The 32-aa MYND domain (for myeloid, Nervy, and Deaf-1) contains non-DNA-binding zinc fingers that are thought to mediate protein-protein interactions (Gross, 1996). (sdbonline.org)
  • What started as a series of microscopic observations in different animal model systems has since evolved into studies of gene expression and regulation, and subsequent protein-protein interactions, cell surface protein expression profiling, and functional mapping of cell fates. (biomedcentral.com)
  • The protein is currently assigned to 297 interactions. (combio.org)
  • Here, we show that the 8th zinc finger motif of MDS1/EVI1 is an oligomerization domain involved not only in interaction of AME with itself but also in interactions with the parental proteins, RUNX1 and MDS1/EVI1, from which AME is generated. (lincoln.ac.uk)
  • RUNX1 mutations contribute to the progression of MDS due to disruption of antitumor cellular defense: a study on patients with lower-risk MDS. (atlasgeneticsoncology.org)
  • How these RUNX1 mutations contribute to the pathobiology of secondary MDS/AML is still unknown. (molcells.org)
  • The interesting thing is that this fusion protein binds to another protein called RUNX1. (nih.gov)
  • purified Runx2, the homolog of Runx1. (wikipedia.org)
  • the binding affinity of RUNX1 and RUNX2 was significantly increased by association with CBFβ. (wikipedia.org)
  • Chromosomal translocations involving the RUNX1 gene are associated with several types of leukemia including M2 AML. (wikipedia.org)
  • RUNX1 was first identified as a RUNX1-ETO fusion protein in acute myeloid leukemia (AML) and is frequently mutated in AML and myelodysplastic syndrome due to chromosomal translocation. (biolegend.com)
  • A rearrangement (translocation) of genetic material involving the RUNX1 gene is found in approximately 7 percent of individuals with a form of blood cancer known as acute myeloid leukemia (AML). (medlineplus.gov)
  • Evaluating the frequency, prognosis and survival of RUNX1 and ASXL1 mutations in patients with acute myeloid leukaemia in northeastern Iran. (atlasgeneticsoncology.org)
  • The contribution of RUNX1 mutations in the pathogenesis of myeloid transformation in ASXL1 -mutated leukemia, however, remains unclear. (biomedcentral.com)
  • The present study demonstrated the biological and functional evidence for the critical role of RUNX1 -MT in ASXL1 -mutated leukemia in the pathogenesis of myeloid malignancies. (biomedcentral.com)
  • Why secondary RUNX1 mutations are associated with sMDS/AML and how they contribute to the pathogenesis of these conditions remains largely unclear. (molcells.org)
  • RUNX1 being the most prevalent translocation whose exact pathogenesis remains unclear. (bvsalud.org)
  • The researchers focused on the most common subtypes of ALL: those harboring ETV6-RUNX1 genetic fusions and those with extra chromosomes, called hyperdiploid. (stjude.org)
  • ETV6-RUNX1 fusion-positive and hyperdiploid each make up 20-25% of pediatric ALL. (stjude.org)
  • In the ETV6-RUNX1 subtype, the researchers found a variant in the gene IGF2BP1 . (stjude.org)
  • The research suggests that the ETV6-RUNX1 fusion revs up expression of IGF2BP1 . (stjude.org)
  • The ETV6-RUNX1 fusion is likely to turn this growth protein back on, contributing to the development of ALL. (stjude.org)
  • RNA binding protein IGF2BP1 synergizes with ETV6-RUNX1 to drive oncogenic signaling in B-cell Acute Lymphoblastic Leukemia. (uclahealth.org)
  • IGF2BP1 is a fetal development protein that normally turns off after birth. (stjude.org)
  • RUNX1 and IGF2BP1 in mouse bone marrow resulted in marrow hypercellularity which was characterized by multi-lineage progenitor expansion and strong Ki67 positivity. (bvsalud.org)
  • RUNX1 and IGF2BP1 was clearly observed. (bvsalud.org)
  • RUNX1 fusion protein and RNA binding protein, IGF2BP1 in activating multiple oncogenic pathways in B-ALL which makes IGF2BP1 and these pathways as attractive therapeutic targets and biomarkers. (bvsalud.org)
  • The translocation, written as t(8;21), combines genetic information from chromosome 21 and chromosome 8, fusing the RUNX1 gene on chromosome 21 with a gene on chromosome 8 called RUNX1T1 (also known as ETO ). (medlineplus.gov)
  • Therefore, the aim of the present study was to investigate the gene expression patterns following siRNA‑mediated suppression of RUNX1‑RUNX1T1 and MAPK1 in Kasumi‑1 and SKNO‑1 cells and to determine the differentially expressed genes in enriched biological pathways. (prinsesmaximacentrum.nl)
  • RUNX1‑RUNX1T1 suppression exerted an anti‑apoptotic effect through the overexpression of BCL2, BIRC3 and CFLAR genes, while MAPK1 suppression induced apopotosis in t(8;21) cells by the apoptotic mitochondrial changes stimulated by the activity of upregulated TP53 and TNFSF10, and downregulated JUN gene. (prinsesmaximacentrum.nl)
  • RUNX1‑RUNX1T1 and MAPK1 depletion induced cell cycle arrest at the G0/G1 phase. (prinsesmaximacentrum.nl)
  • Accumulation of cells in the G1 phase was largely the result of downregulated expression of TBRG4, CCNE2, FOXO4, CDK6, ING4, IL8, MAD2L1 and CCNG2 in the case of RUNX1‑RUNX1T1 depletion and increased expression of RASSF1, FBXO6, DADD45A and P53 in the case of MAPK1 depletion. (prinsesmaximacentrum.nl)
  • In humans, the gene RUNX1 is 260 kilobases (kb) in length, and is located on chromosome 21 (21q22.12). (wikipedia.org)
  • Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)
  • Higher RUNX1 expression was found to correlate with decreased median overall survival and disease-free survival in The Cancer Genome Atlas (TCGA) data set of high grade serous ovarian cancer (HGSOC) patients. (techscience.com)
  • The mutant proteins often retain some of their capabilities but are no longer sensitive to the controls that regulate the normal form of the protein. (cancerquest.org)
  • We analysed RUNX1 mutant AML patients compared to non-mutant patients using an integrated multi-omics, multi-database analysis of exome, and transcriptomics data. (mdpi.com)
  • Co-expression of two mutant genes increased myeloid stem cells in animal model, suggesting that cooperation of RUNX1 and ASXL1 mutations played a critical role in leukemia transformation. (biomedcentral.com)
  • Moreover, the RUNX1 mutant protein was more stable than WT and increased HIF1-α and its target ID1 gene expression in ASXL1 mutant cells. (biomedcentral.com)
  • Here, we will discuss the current insights and ideas regarding mutant RUNX1 in the context of malignant transformation of iBMFs, taking SCN as the leading example. (molcells.org)
  • The RUNX1 gene mutations associated with these diseases are somatic mutations and are not inherited. (medlineplus.gov)
  • Somatic RUNX1 mutations are found in approximately 10% of patients with de novo acute myeloid leukemia (AML), but are more common in secondary forms of myelodysplastic syndrome (MDS) or AML. (molcells.org)
  • De novo acute myeloid leukemia (AML), type M2 with monocytosis or M4 in the case tested for RUNX1. (atlasgeneticsoncology.org)
  • RUNX1 and RUNX3 Genes Expression Level in Adult Acute Lymphoblastic Leukemia-A Case Control Study. (atlasgeneticsoncology.org)
  • Monitoring minimal residual disease in RUNX1-mutated acute myeloid leukemia. (atlasgeneticsoncology.org)
  • Genome profiling of chronic myelomonocytic leukemia: frequent alterations of RAS and RUNX1 genes. (atlasgeneticsoncology.org)
  • Runx1 was purified as a sequence-specific DNA-binding protein that regulated the disease specificity of the Moloney murine Leukemia virus. (wikipedia.org)
  • His research has led to the identification of several non-coding RNA species and RNA-binding proteins that are important in acute leukemia. (uclahealth.org)
  • Previously, we observed that transcription factor RUNX1 mutations ( RUNX1 -MT) coexisted with ASXL1 -MT in CMML and at myeloid blast phase of chronic myeloid leukemia. (biomedcentral.com)
  • A new study led by researchers at Memorial Sloan Kettering Cancer Center (MSKCC) reports on a novel mechanism that can enhance the function of a protein that is frequently impaired in patients with acute forms of leukemia. (mskcc.org)
  • RUNX1 itself is the target of several chromosome rearrangements seen in leukemia. (nih.gov)
  • Furthermore, we observed that inhibition of RUNX1/ETO in Kasumi1 cells and in RUNX1/ETO positive primary acute myeloid leukemia patient samples leads to up-regulation of miR144/451. (uni-frankfurt.de)
  • RUNX1-positive leukemia cells in preclinical models. (lu.se)
  • We will discuss three key proteins that function as tumor suppressors implicated in the development of pediatric (and some adult) cancers: pRB, p53, and PTEN. (medscape.com)
  • RB1 encodes the protein pRB and was the first tumor suppressor gene to be molecularly defined. (medscape.com)
  • This protein interacts with another protein called core binding factor beta or CBFβ (produced from the CBFB gene), which helps RUNX1 bind to DNA and prevents it from being broken down. (medlineplus.gov)
  • And when they have this inversion, they always have a fusion protein, CBFb-SMMHC. (nih.gov)
  • We evaluated the expression of SALL4 using single-cell mass cytometry (CyTOF) utilizing 28 antibodies including surface lineage markers and intracellular proteins, such as p53, ki67, c-myc and pAKT to identify SALL4 expressed cells and related pathway in bone marrow (BM) for 10 MDS patients. (confex.com)
  • Mice transduced with both ASXL1 and RUNX1 mutations enhanced inhibitor of DNA binding 1 (ID1) expression in the spleen, liver, and bone marrow cells. (biomedcentral.com)
  • Bone marrow samples from CMML showed that ID1 overexpressed in coexisted mutations of RUNX1 and ASXL1 compared to normal control and either RUNX1 -MT or ASXL1 -MT samples. (biomedcentral.com)
  • RUNX1 plays a crucial role in adult (definitive) haematopoiesis during embryonic development. (wikipedia.org)
  • RUNX1 positive patients in an Indian cohort of pediatric ALL (n=167) with a positive correlation with prednisolone resistance. (bvsalud.org)
  • Sexually Dimorphic Increases in Bone Mass Following Tissue-specific Overexpression of Runx1 in Osteoclast Precursors. (atlasgeneticsoncology.org)
  • This is key not only to understand how carcinogenesis appears and develops in these malignancies to be able to early diagnose them, but also to open up the possibility to employ therapeutic strategies targeting a driver protein to counteract the alteration of another connected driver. (biomedcentral.com)
  • RUNX1 plays an indispensable role in definitive hematopoiesis and hematological malignancies and as an emerging therapeutic target in cardiovascular disease. (lipexogen.com)
  • Mice embryos with homozygous mutations on RUNX1 died at about 12.5 days. (wikipedia.org)
  • Analyses of mice with a conditional knock-out of Runx1 in VGLUT3 lineage neurons demonstrate that Runx1 is pivotal to the development of VGLUT3-persistent neurons, such as the expression of VGLUT3 and TH and the formation of the longitudinal lanceolate endings. (jneurosci.org)
  • At 9 months post-BMT, mice harboring combined RUNX1 and ASXL1 mutations developed disease characterized by marked splenomegaly, hepatomegaly, and leukocytosis with a shorter latency. (biomedcentral.com)
  • ChIP-sequencing analysis, using antibodies recognising PRMT5, LMO2 and RUNX1, showed overlapping peaks in T-ALL, confirming a functional relationship between these proteins. (bham.ac.uk)
  • Current treatments to control retinal neovascularization require injecting very large proteins, including antibodies, into the eyes of patients as often as once a month," notes co-corresponding author Joseph Arboleda-Velasquez, M.D., Ph.D., in a study news release . (aoa.org)
  • All three family proteins physically associate with SMAD proteins, downstream targets of TGF-beta/BMP signalling, and play roles in mammalian development [ 7 ]. (hindawi.com)
  • HEK293FT cells were transfected with RUNX1-TAG-Puro plasmid along with cDNA encoding human RUNX1 or an empty vector (pcDNA). (lipexogen.com)
  • The RUNX1 cDNA vector (RUNX1-P2A-RFP) contains RFP downstream of RUNX1 separated by a P2A sequence. (lipexogen.com)
  • RUNX1 cDNA expression vectors is also available upon request. (lipexogen.com)
  • Although the protein encoded by runt was demonstrated to exhibit nuclear translocation, it was not yet established that this protein is a transcription factor. (wikipedia.org)
  • Furthermore, Runx1 is required to establish mechanosensitivity in C-LTMRs, by controlling the expression of the mechanically gated ion channel Piezo2. (jneurosci.org)
  • The methylation status and protein expression levels of RUNX3 were measured by methylation-specific PCR and Western blot in 136 and 72 different malignancy grade glioma tissues, respectively. (hindawi.com)
  • The results demonstrate that RUNX3 gene methylation and protein expression downregulation are glioma malignancy dependent and contribute to tumour progression. (hindawi.com)
  • The expression of SALLA&B is moderately correlated with NAT10 (γ=0.47) and RUNX1 (γ=0.46). (confex.com)
  • Augmentations in foxp3 protein expression and decreases in runx1 and foxp3 mRNA occurred concurrently with expression of miR-210 following dermal TDI exposure. (cdc.gov)
  • The human hemopoietic cell kinase (HCK) is a member of the src family of protein tyrosine kinases specifically expressed in myeloid cells and to a minor extent in B-lymphoid cells. (uni-frankfurt.de)
  • The protein RUNX1 is composed of 453 amino acids. (wikipedia.org)
  • RUNX1 was found to be rearranged in the leukemic cell DNAs from t(8;21)(q22;q22) AML patients. (wikipedia.org)
  • A cryptic inv(21)(q21q22) associated with a microdeletion at one of the breakpoints, and a fusion involving RUNX1 and USP16 (encoding a de-ubiquitinating enzyme). (atlasgeneticsoncology.org)
  • RUNX1 regulates CD4 gene transcription during multiple stages of T cell development and represses the CD4 gene in CD4-CD8- (double negative) T cells. (biolegend.com)
  • Inversion of the 21q21.3-q22.12 region and generation of USP16-RUNX1 gene fusion. (atlasgeneticsoncology.org)
  • The USP16-RUNX1 gene fusion characterized by RT-PCR is explained by the inversion of the central interval [BP2-BP3]. (atlasgeneticsoncology.org)
  • Sequence analysis showed that the result of the inversion/fusion generated a chimeric USP16-RUNX1 transcript. (atlasgeneticsoncology.org)
  • The USP16-RUNX1 fusion transcript did not have an open reading frame using the canonical start codons of USP16 or RUNX1. (atlasgeneticsoncology.org)
  • The break/fusion was not present in the germline since we did not find the USP16-RUNX1 transcript in buccal smear cells of the patient. (atlasgeneticsoncology.org)
  • The fusion protein blocks normal hematopoiesis. (nih.gov)
  • RUNX1 fusion protein. (bvsalud.org)
  • RUNX1 fusion transcript itself. (bvsalud.org)
  • Together, these proteins form one version of a complex known as core binding factor (CBF). (medlineplus.gov)
  • Our project shows the interaction of the LMO2 multiprotein complex with both PRMT5 and RUNX1. (bham.ac.uk)
  • The identification of dimethylated arginine RUNX1 indicates a possible regulatory function for PRMT5, which may be related to their interaction with the LMO2 complex. (bham.ac.uk)
  • This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag. (cusabio.com)
  • The transcription of RUNX1 is regulated by 2 enhancers (regulatory element 1 and regulatory element 2), and these tissue specific enhancers enable the binding of lymphoid or erythroid regulatory proteins, therefore the gene activity of RUNX1 is highly active in the haematopoietic system. (wikipedia.org)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • This was confirmed by nested PCR amplification of reverse-transcribed RNA from the patients BM cells, which detected a 245 bp-long USP16-RUNX1 transcript. (atlasgeneticsoncology.org)
  • Genes whose protein products stimulate or enhance the division and viability of cells. (cancerquest.org)
  • Within the brain, the protein is involved in the development of specialized cells that process smell. (biolegend.com)
  • Cells with PIGA mutations lack a vast class of proteins which act to protect blood cells from being destroyed and fight infection. (aamds.org)
  • Clinical problems experienced by patients with PNH are the result of cells lacking those proteins. (aamds.org)
  • Clinical problems experienced by patients with PNH are the result of cells lacking these proteins. (aamds.org)
  • Electrophoretic mobility shift analysis using nuclear extracts obtained from RAW264 cells and from the promonocytic cell line U-937 revealed the formation of at least three distinct protein-DNA complexes at each of these sites, one of which was found to contain the transcription factor Sp1. (uni-frankfurt.de)
  • GFP reporter activation in HEK293FT cells co-transfected with RUNX1 -TAG-Puro reporter and human RUNX1. (lipexogen.com)