These three-E. ewingii, E. chaffeensis, and E. canis-form a group in terms of relatedness within the genus Ehrlichia. (wikipedia.org)
The name was proposed to be Ehrlichia ewingii, named after S.A. Ewing who initially identified the parasitic organism. (wikipedia.org)
Since Ehrlichia ewingii is unable to synthesize all the organic compounds required for growth, also known as an auxotroph, the bacterium requires a host for growth and survival. (wikipedia.org)
Ehrlichia ewingii is specifically transmitted by the North American Tick, Amblyomma americanum. (wikipedia.org)
Currently, Ehrlichia ewingii cannot be cultivated in a number of cell lines, meaning that study of this organism is dependent on extraction from arthropod and vertebrate hosts. (wikipedia.org)
Ehrlichia ewingii is a human pathogen which results in a serious infection if not treated in a timely manner. (wikipedia.org)
This is partially due to the difficulty diagnosing the causative agent of infection, however further Ehrlichia ewingii research could allow better diagnostic tests to identify ehrlichial infections before they progress to severe cases. (wikipedia.org)
Recognized species include E. canis, E. chaffeensis, E. ewingii, E. muris, and E. ruminantium ( 1 ). (cdc.gov)
E.canis, E. chaffeensis, and E. ewingii ) by using the ELISA-based assay SNAP 4DxPlus (IDEXX Laboratories, Inc., Westbrook, ME, USA) according to the manufacturer's instructions ( 9 ). (cdc.gov)
The prevalence of Ehrlichia chaffeensis and Ehrlichia ewingii combined in host-seeking adults declined significantly from 47 % at the time the A. americanum population was discovered in 2017 to 7% in 2020. (bvsalud.org)
Ehrlichia ewingii can also infect humans. (google.com)
Ticks were examined for Ehrlichia and Anaplasma by nested PCR directed at the gltA, antigen-expressing gene containing a variable number of tandem repeats, 16S rRNA, and groESL genes. (cdc.gov)
Ticks5
Although equids are not known hosts for E. chaffeensis, bacterial DNA has been amplified from ticks ( Dermacentor nitens and Amblyomma cajennense ) collected from horses in Panama ( 8 ). (cdc.gov)
This study aimed to conduct a molecular-based taxonomic and ecological characterization of uncultured Ehrlichia species or genotypes from ticks in Japan. (bvsalud.org)
We first surveyed 616 Haemaphysalis ticks by p28-PCR screening and analyzed five additional housekeeping genes (16S rRNA, groEL, gltA, ftsZ, and rpoB) from 11 p28-PCR-positive ticks. (bvsalud.org)
To determine if E. chaffeensis is present in ticks at Aberdeen Proving Ground, MD, questing A. americanum ticks were collected from 33 sites. (google.com)
rates of infection of amblyomma americanum (l.) by ehrlichia chaffeensis were compared in 100 ticks collected from sites in each of four states: indiana, north carolina, kentucky, and mississippi. (liverpool.ac.uk)
Members of the genus2
However, the organism is assumed to have a similar metabolism to members of the genus due to the high similarity between functional 16S rRNA gene sequencing and the highly related infections they cause within cells. (wikipedia.org)
These and other historical factors resulted in substantial limitations in our understanding of infections caused by members of the genus Ehrlichia . (vin.com)
Monocytic ehrlichiosis3
In North America, cervids are reservoir hosts for E. chaffeensis, which after tick transmission causes monocytic ehrlichiosis in humans ( 1 ). (cdc.gov)
From blood samples, 16S rRNA genes were amplified using standard polymerase chain reaction (PCR), and the genes were analyzed using gel electrophoresis and a GAP (Genetic Analysis Program) system. (wikipedia.org)
Sequencing of 16S rDNA, sodB, and groEL genes indicated that the bacterium is most likely a novel Ehrlichia species. (cdc.gov)
Ehrlichiosis3
on June 18, 1987 using a canine diagnosed with granulocytic ehrlichiosis: an infection of the granulocytes by a member of the Ehrlichia sp. (wikipedia.org)
Until the recent identification of monocytic (2) and neutrophilic (3,4) human ehrlichiosis in Japan, Sweden, and the United States, Ehrlichia species were considered solely animal pathogens, for which limited interest or funding were available for research studies. (vin.com)
Species6
This data was compared to all other Ehrlichia species, and Anderson et al. (wikipedia.org)
Ehrlichia species are obligatory intracellular bacteria transmitted by arthropods, and some of these species cause febrile diseases in humans and livestock. (bvsalud.org)
Genome sequencing has only been performed with cultured Ehrlichia species, and the taxonomic status of such ehrlichiae has been estimated by core genome-based phylogenetic analysis. (bvsalud.org)
Rickettsial organisms in general, and Ehrlichia species in particular, are difficult to isolate from animals or human patients using conventional tissue culture systems. (vin.com)
Of comparative medical interest, cats, dogs, humans, and other domestic and wild animal species can all be infected with the same Ehrlichia sp. (vin.com)
A. phagocytophilum and A. platys ), B. burgdorferi sensu stricto, and Ehrlichia spp. (cdc.gov)
Polymerase chain re1
Sequences diagnostic for E. chaffeensis from three different loci (16S rRNA, 120-kDa protein, and a variable-length polymerase chain reaction [PCR] target, or VLPT) were targeted for amplification by the PCR. (google.com)
Infect1
The results of the present study suggest that multiple ehrlichial agents infect dogs in Brazil, which highlights the need to consider different Ehrlichia spp. (bvsalud.org)
Phagocytophilum2
Sequence analysis of the 16S rRNA gene confirmed two positives as A.phagocytophilum-human agent variant. (cdc.gov)
DNA from A. phagocytophilum variant 4 (of 16S rRNA) was found in multiple and sequential samples. (biomedcentral.com)
Replication1
Although the genome itself has not been sequenced, the use of the host cell for replication is known based on comparisons to other Ehrlichia sp. (wikipedia.org)
6 , 7 ) in horse serum, no reports have documented isolation or PCR detection of Ehrlichia spp. (cdc.gov)
Canine1
Due to the variable location, this strain was called Canine Granulocytic Ehrlichia (CGE). (wikipedia.org)
Found1
Furthermore, we found that V1 in the V1-V9 regions of Ehrlichia 16S rRNA exhibited the greatest variability. (bvsalud.org)
Brazil1
Although studies from Brazil and Oklahoma (USA) have documented reactivity to Ehrlichia spp. (cdc.gov)
Samples1
According to assay results, 51 (55%) horse serum samples were Ehrlichia spp. (cdc.gov)
Presence1
Fifty-two percent of the collection sites yielded pools infected with E. chaffeensis, confirming the presence and widespread distribution of E. chaffeensis at Aberdeen Proving Ground. (google.com)
Each run included negative controls and 2 positive recombinant DNA controls (a B. miyamotoi 16S rRNA gene fragment and an internal control having 10 4 -10 6 copies/mL) as standards. (medscape.com)
However, the organism is assumed to have a similar metabolism to members of the genus due to the high similarity between functional 16S rRNA gene sequencing and the highly related infections they cause within cells. (wikipedia.org)
While the overall level of DNA-DNA homology was approximately 11%, sequence alignment of the two 16S rRNA genes yielded a homology value of more than 95%, emphasizing the highly conserved nature of the 16S rRNA gene. (microbiologyresearch.org)
Genomic DNA was extracted from a subsample, and following the amplification of the bacterial 16S rRNA gene V3-V4 hypervariable region, next-generation sequencing (NGS) on the Illumina MiSeq platform was used to assess the microbial composition. (biomedcentral.com)
DNA and RNA-based sequencing of the 16S rRNA gene and transcripts had been used to evaluate the phylogenetic range of microbial communities at property experiencing corrosion in an oil manufacturing facility. (cdna-clone.com)
Neorickettsia1
In the 2000s, new rickettsial pathogens of veterinary importance that have been identified include Ehrlichia minasensis, Neorickettsia findlayensis, and Ehrlichia japonica . (veteriankey.com)
Sequences2
The E. chaffeensis 16S rRNA base and GroEL amino acid sequences from Japan were most closely related to those of E. chaffeensis Arkansas. (nih.gov)
To determine whether Ehrlichia chaffeensis exists in Japan, we used PCR to examine blood from sika deer in Nara, Japan. (nih.gov)
We estimated the number of copies of B. miyamotoi and B. burgdorferi sensu lato DNA and RNA in PCR-positive patient blood samples by using a qPCR for 16S rRNA. (medscape.com)