Roles mice knockout. Medical search. Frequent questions

The gut microbiome and its metabolites play important roles in bile acid metabolism, and previous studies have indicated the association of the gut microbiome with ICP. (bvsalud.org)
This understanding of the determinants of HDL particle size and protein cargo distribution serves as a basis for determining the roles of HDL subpopulations in metabolism and disease states. (cdc.gov)

Our research includes the use of knockout mice and zebrafish as laboratory models. (ncl.ac.uk)
Likewise, loss of Caskin2 in vivo promotes increased vascular branching and permeability in mouse and zebrafish models. (duke.edu)

Therefore, it might be safe to say that host CCL5 plays important roles in controlling cancer development but might create either pro- or anticancer environments according to the given situation, such as the type of cells that produce CCL5 and the type of cancers. (nature.com)
In addition to Cd detoxication, MT-transgenic and MT-null mice are also good models to determine other functions of MT. MT plays important roles in maintaining Zn homeostasis and protection against Zn toxicity. (go.jp)
SF-1 also plays important roles in reproduction at all three levels of the hypothalamic-pituitary-gonadal axis. (duke.edu)
Although the precise molecular mechanisms of these effects remain unclear, we have shown that Caskin2 interacts with several proteins known to have important roles in endothelial biology and cardiovascular disease including the serine/threonine phosphatase PP1, the endothelial receptor Tie1, and eNOS, which is a critical regulator of vascular homeostasis. (duke.edu)

Scientists at The Scripps Research Institute are reporting that mice created to lack a certain type of molecule known as an LPA receptor have fertility problems, which suggests that these receptors play a major role in conception. (sciencedaily.com)
In the latest issue of the journal Nature, the scientists detail how mice that lack LPA receptors, which normally appear on the surface of cells in a mouse's womb, have fertility problems. (sciencedaily.com)
The discovery that the LPA receptors affect fertility in mice may open a new area of fertility research and treatment for humans. (sciencedaily.com)
Here we investigated the role of each receptor in the setting of cecal ligation and puncture-induced sepsis by using antibody-induced blockade of C5a receptors and knockout mice. (uni-luebeck.de)

The mouse basal cell carcinomas and trichoblastoma-like tumors resemble human basal cell carcinomas in their loss of normal hemidesmosomal components, presence of p53 mutations, frequent loss of the normal remaining Ptch allele, and activation of hedgehog target gene transcription. (nature.com)
The Ptch mutant mice provide the first mouse model, to our knowledge, of ultraviolet and ionizing radiation-induced basal cell carcinoma-like tumors, and also demonstrate that Ptch inactivation and hedgehog target gene activation are essential for basal cell carcinoma tumorigenesis. (nature.com)
In the present review, the phenotypes of Met gene‑modified mice are summarized. (spandidos-publications.com)
The X-linked gene Rlim plays major roles in female mouse development and reproduction, where it is crucial for the maintenance of imprinted X chromosome inactivation in extraembryonic tissues of embryos. (umass.edu)
First identified as a tissue-specific regulator of the transcription of the cytochrome P450 steroid hydroxylases, considerably broader roles for SF-1 were revealed by genetic studies in mice lacking SF-1 due to targeted gene disruption. (duke.edu)
Here, we describe a complete knockout mouse model of the autism-associated Shank3 gene, with a deletion of exons 4-22 (Δe4-22). (duke.edu)

Blocking F-K1057/1079 with phenylalaninol sensitizes insulin signaling and relieves T2D symptoms in hFARS-transgenic and db/db mice. (bvsalud.org)
1999. Adverse reproductive outcomes in the transgenic Ah receptor-deficient mouse. (cdc.gov)

C57BL/6 mice received 10 5 red blood cells infected with Plasmodium berghei ANKA intraperitoneally. (hindawi.com)
Inbred male C57BL/6 mice (8 weeks old) were used. (hindawi.com)

In situ detection of hedgehog target genes and immunohistology of Ptch +/- mouse BCC differentiation markers, hemidesmosomal proteins and Bcl2 protein. (nature.com)
Central administration of NmU also induces expression of key genes in hypothalamic areas associated with stress, as well as stress-related behaviours that can be blocked by CRH antagonist (this is absent from CRH knockout mice). (wikipedia.org)
2015. Cytochrome P450 1b1 in polycyclic aromatic hydrocarbon (PAH)-induced skin carcinogenesis: Tumorigenicity of individual PAHs and coal-tar extract, DNA adduction and expression of select genes in the Cyp1b1 knockout mouse. . (oregonstate.edu)
Although carcinogenic roles for the INK4B, INK4C, INK4D, CIP1, KIP1, and KIP2 genes appear to be limited, INK4A is among the most commonly mutated genes in human tumors. (medscape.com)

Conditional Met KO mice were generated using Cre‑loxP methodology and characterization of these mice indicated that the HGF‑Met signaling pathway is essential in regeneration, protection, and homeostasis in various tissue types and cells. (spandidos-publications.com)
Conditional knockout of Gli3 causes a significant larger brain size, which can be recovered by knockdown of miR-7. (frontiersin.org)
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects. (creativebiomart.net)

These new findings suggest new roles for L1 in the mechanism of cortical dendrite differentiation, as well as in guidance of callosal axons and regulation of hippocampal development. (jneurosci.org)
These studies delineated essential roles of SF-1 in regulating endocrine differentiation and function at multiple levels. (duke.edu)

These mice are able to produce eggs normally, so that the eggs can be fertilized, but the resulting embryos, which are otherwise healthy, have problems implanting in the womb -- the last step in conception. (sciencedaily.com)
Now Chun and his colleagues have discovered a new molecular pathway that influences fertility, at least in mice -- and one that directly affects the ability of mouse embryos to implant in their mother's womb. (sciencedaily.com)
1991. Effects of polychlorinated biphenyls with Ah receptor affinity on lymphoid development in the thymus and the bursa of Fabricius on chick embryos in ovo and in mouse thymus anlagen in vitro . (cdc.gov)

These SF-1-knockout mice had agenesis of their adrenal glands and gonads, male-to-female sex reversal of their internal and external genitalia, impaired gonadotrope function, and agenesis of the ventromedial hypothalamic nucleus. (duke.edu)

However, its physiological and pathophysiological roles in vivo remain unknown. (nih.gov)
Fgf-23(-/-) or Klotho(-/-) knockout mice exhibit several pathophysiological processes consistent with premature aging including severe atrophy of tissues. (harvard.edu)

Interestingly, despite the severe defect in glucose-induced insulin secretion, Kir6.2 knockout mice show only a very mild impairment in glucose tolerance. (diabetesjournals.org)
However, when the knockout mice become obese with age, they develop fasting hyperglycemia and glucose intolerance, while neither fasting hyperglycemia nor glucose intolerance is evident in the aged knockout mice without obesity, suggesting that both the genetic defect in glucose-induced insulin secretion and the acquired insulin resistance due to environmental factors are necessary to develop diabetes in Kir6.2 knockout mice. (diabetesjournals.org)
β-cell-specific Piezo1-knockout mice show impaired glucose tolerance in vivo and reduced glucose-induced insulin secretion, β-cell electrical activity and Ca 2+ elevation in vitro. (lu.se)

Interestingly, a mouse ortholog of HAS2-AS1, Has2as, clearly exhibited a differential splicing pattern among three major splice variants in the Pnn-deficient mouse cornea. (molvis.org)

We look at the function of novel muscle proteins and their roles in pathogenesis. (ncl.ac.uk)
The unexpected roles for ULK1 and ULK2 in promoting ER-to-Golgi trafficking of proteins, which seem to have been conserved throughout evolution, appear to be essential for maintaining cellular homeostasis and the integrity of the cell. (neurosciencenews.com)
Neurotransmission relies on repeated cycles of exo- and endocytosis of the synaptic vesicles (SVs), in which several palmitoylated proteins play critical roles. (jci.org)
Using postmortem brain tissues from an INCL patient and tissue from the PPT1-knockout (PPT1-KO) mice that mimic INCL, we report here that PPT1 deficiency caused persistent membrane anchorage of the palmitoylated SV proteins, which hindered the recycling of the vesicle components that normally fuse with the presynaptic plasma membrane during SV exocytosis. (jci.org)
2007). These proteins are important for the regulation of intracellular pH (pH i ) and play crucial roles in the epithelial absorption of HCO 3 - (e.g., in the renal proximal tubule) and secretion of HCO 3 - (e.g., in the pancreatic duct). (tcdb.org)

Mice fed phenylalanine-rich chow or phenylalanine-producing aspartame or overexpressing human phenylalanyl-tRNA synthetase (hFARS) develop insulin resistance and T2D symptoms. (bvsalud.org)
Yet, PIEZO1 expression is elevated in islets from human donors with type-2 diabetes (T2D) and a rodent T2D model (db/db mouse), in which insulin secretion is reduced. (lu.se)

To determine the physiological roles of K(ATP) channels directly, we have generated two kinds of genetically engineered mice: mice expressing a dominant-negative form of Kir6.2 specifically in the pancreatic beta-cells (Kir6.2G132S Tg mice) and mice lacking Kir6.2 (Kir6.2 knockout mice). (diabetesjournals.org)
These data provide new insights into the physiological roles of FGF-23 and Klotho. (harvard.edu)

Combined deficiency of PI3KC2α and PI3KC2β reveals a nonredundant role for PI3KC2α in regulating mouse platelet structure and thrombus stability. (nih.gov)

The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
Mice were culled on day 7 after infection and blood and spleen cell phenotype and activation were evaluated. (hindawi.com)

Macrophage stimulating protein (MSP) was discovered as a serum protein that promoted mouse macrophage motility ( 13 ), and was later purified to homogeneity from human plasma ( 14 ). (spandidos-publications.com)
Recombinant Mouse IL1A full length or partial length protein was expressed. (creativebiomart.net)
2009. Activator protein 2alpha suppresses intestinal tumorigenesis in the Apc(min) mouse. . (oregonstate.edu)
2011. Activator protein-2α functions as a master regulator of multiple transcription factors in the mouse liver. . (oregonstate.edu)
Here we demonstrate that the previously uncharacterized scaffolding protein Caskin2 is a novel regulator of EC quiescence and that loss of Caskin2 in mice results in elevated blood pressure at baseline. (duke.edu)

However, the NmUR2 KO mice exhibit reduced pain sensitivity in both hot plate test and the chronic phase of the formalin test. (wikipedia.org)

We report here that Ptch +/- mice develop primordial follicular neoplasms resembling human trichoblastomas, and that exposure to ultraviolet radiation or ionizing radiation results in an increase in the number and size of these tumors and a shift in their histologic features so that they more closely resemble human basal cell carcinoma. (nature.com)
2017. Divergent roles of p120-catenin isoforms linked to altered cell viability, proliferation, and invasiveness in carcinogen-induced rat skin tumors. . (oregonstate.edu)
[ 2 ] Targeted disruption of TP53 in the mouse leads to the development of various tumors (see image below). (medscape.com)
Tumors that developed in mice deficient in INK4A were enhanced by the topical application of carcinogens and ultraviolet light. (medscape.com)
1991. Promotion of mouse lung tumors by bioaccumulated polychlorinated aromatic hydrocarbons. (cdc.gov)

In a number of cases, extant TE sequences have integrated with established genomic functions and been co-opted by the genome for critical roles [ 7 ],[ 8 ]. (biomedcentral.com)

In addition to its roles in the regulation of inflammatory diseases and the maintenance of local immune cells, CCL5 expressed by cancer cells plays diverse roles in shaping cancer microenvironments toward their own survival. (nature.com)
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes. (creativebiomart.net)
and they play roles in human genetic diseases, with functional disorders causing diseases. (tcdb.org)

We generated an inducible liver-specific Reptin knockout ( Repin LKO ) mouse model. (nih.gov)
Using a novel, myeloid-specific, Zip8 knockout model, we identified vital roles of ZIP8 in macrophage and DC function upon pneumococcal infection. (cdc.gov)
Thus, Kir6.2G132S Tg mice and Kir6.2 knockout mice provide a model of type 2 diabetes and clarify the various roles of K(ATP) channels in endocrine pancreatic function. (diabetesjournals.org)
In the study, a mouse CNV model was generated by laser photocoagulation. (molvis.org)
Altered mGluR5-Homer scaffolds and corticostriatal connectivity in a Shank3 complete knockout model of autism. (duke.edu)

and Rando, Oliver J., "Deficient Spermiogenesis in Mice Lacking Rlim" (2021). (umass.edu)

Direct evidence linking the INK4A locus to tumorigenesis was provided by the targeted disruption of exon 2 of INK4A in mice. (medscape.com)

In light of the severe motor phenotypes and apparent cerebellar abnormalities seen in BFPP patients, we studied the cerebellum of Gpr56 knock-out mice. (jneurosci.org)

Gpr56 −/− mice display a severe malformation of the rostral cerebellum that develops perinatally. (jneurosci.org)

Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions. (creativebiomart.net)

In contrast to NmU peptide-deficient mice, NmUR2 knockout (KO) mice appeared normal with regard to stress, anxiety, body weight regulation, and food consumption. (wikipedia.org)

Although the researchers were expecting autophagy to be defective when both enzymes were impaired, they were surprised stress arose in the ER that resulted in selective loss of neurons in the brains of mice (hippocampal neurons). (neurosciencenews.com)

10)genetic background of mouse strains, rather than constitutive MT levels, is a more important determinant for Cd-induced acute testicular injury. (go.jp)

In contrast, chronic CCl(4) administration induces a similar degree of liver injury in iNKT-deficient and wild-type mice, and only a slightly higher grade of liver fibrosis in iNKT-deficient mice than wild-type mice 2 weeks but not 4 weeks after CCl(4) injection, although iNKT cells are able to kill activated stellate cells. (nih.gov)
Administration of S. pneumoniae into the lung resulted in increased inflammation, morbidity, and mortality in Zip8 knockout mice compared with wild-type counterparts. (cdc.gov)
We have previously showed that OB are attracted to DC-STAMP+ OC proximity at bone fracture sites in wild-type mice, a phenomena which was not detected in DC-STAMP KO mice. (rochester.edu)
2017. Dibenzo[def,p]chrysene transplacental carcinogenesis in wild-type, Cyp1b1 knockout, and CYP1B1 humanized mice. . (oregonstate.edu)

Deletion of the proximal enhancer decreases CCL5 expression and augments the cytotoxic activity of tissue-resident T and NK cells, which coincides with reduced melanoma metastasis in mouse models. (nature.com)
On the contrary, recent studies using CCL5 knockout mice showed that the total absence of host CCL5 exhibits increased antitumor immunity in breast cancer models, proposing a unique procancer role of host CCL5 20 , 21 . (nature.com)

Both NmUR2 expression and NmU-23 binding sites are highly localized to the outer layers of the spinal dorsal horn, and administration of NmU via intracerebroventricular (ICV) injections usually increases pain sensitivity in rats and mice. (wikipedia.org)
ICV injections of NmU in rats and mice induce behavior responses associated with activation of the nociceptive pathways, for example it will increase plasma levels of corticosterone, and stimulates the release of CRH from hypothalamic explants in vitro. (wikipedia.org)
Carcinogenicity and chronic toxicity in rats and mice exposed to chloroform by inhalation. (who.int)

Diverse roles of K(ATP) channels learned from Kir6.2 genetically engineered mice. (diabetesjournals.org)

Recent studies using neutralizing anti-CCL5 antibodies have suggested that the constitutive expression of CCL5 plays a regulatory role in maintaining tissue-resident memory (Trm) T cells in the human vaginal tract 9 and mouse skin 10 , suggesting that constitutive CCL5 expression in noninflamed conditions, hereafter referred to as homeostatic CCL5 expression, might be important for homeostasis of tissue-resident lymphocytes. (nature.com)
What experimental approaches allow researchers to dissect the paradoxical roles of IL-1α as both a pro-inflammatory and tissue repair mediator in distinct contexts? (creativebiomart.net)

Mechanical forces are critical for embryonic development - yet very little is known about their roles. (royalsociety.org)
1994. Ah receptor in embryonic mouse palate and effects of TCDD on receptor expression. (cdc.gov)

Knockdown of microRNA (miRNA) miR-7 can rescue abnormal brain morphology in Gli3 knockout mice by recovering progenitor specification, neuronal production and migration through a counter-balance of the Gli3 activity. (frontiersin.org)

Here we show that iNKT-deficient mice are more susceptible to carbon tetrachloride (CCl(4))-induced acute liver injury and inflammation. (nih.gov)

Furthermore, the results of studies using HGF‑overexpressing mice have indicated the therapeutic potential of HGF for various types of disease and injury. (spandidos-publications.com)

The effect of NmU on stress and pain perception pathways has been demonstrated using mice. (wikipedia.org)

Enlarged ventricles and septal abnormalities were also features of the mutant mouse brain. (jneurosci.org)

Scholars@Duke publication: Steroidogenic factor 1 plays multiple roles in endocrine development and function. (duke.edu)
Using single-cell RNA sequencing (RNAseq), we studied the development of ProSG, their SG descendants and testicular somatic cells during the perinatal period in mice. (biologists.com)

In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
Methods: Total RNA was isolated from PNN knockdown human corneal epithelial (HCET) cells or Pnn-deficient mouse corneas, and subjected to real-time-PCR (RT-PCR) assays, and the alternatively spliced lncRNAs were counted. (molvis.org)

2019. Lipidomic and transcriptomic analysis of western diet-induced nonalcoholic steatohepatitis (NASH) in female Ldlr -/- mice. . (oregonstate.edu)

Organisms2

Analytical, Diagnostic and Therapeutic Techniques and Equipment1

Phenomena and Processes1

Metabolism2

Zebrafish2

Important roles4

Receptors4

Gene6

Transgenic2

C57BL2

Genes4

Conditional3

Differentiation2

Embryos3

Agenesis1

Pathophysiological2

Glucose3

Interestingly1

Proteins5

Insulin2

Physiological roles2

Deficiency1

Phenotype2

Protein5

Exhibit1

Tumors5

Critical roles1

Diseases3

Model5

20211

Disruption1

Phenotypes1

Malformation1

Molecular1

Regulation1

Hippocampal1

Genetic background1

Wild-type4

Models2

Rats3

Diverse1

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Specification1

Inflammation1

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