Role of osteocalcin phenotype osteocalcin. Medical search. Frequent questions

We have conducted large population studies relating serum osteocalcin levels to race, gender, normal growth, development, and aging and to a variety of metabolic bone diseases including osteoporosis. (yale.edu)
Associations of Serum Osteocalcin and Polymorphisms of the Osteocalcin Gene with Bone Mineral Density in Postmenopausal and Elderly Chinese Women. (cdc.gov)

To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. (fluoridealert.org)
Considering that CsinCPI-2 induced the differentiation of human dental pulp cells into osteogenic phenotype, it is important to know the signaling pathways involved in this differentiation, for a better understanding of the effect of the protein on undifferentiated mesenchymal cells and, consequently, to indicate it or not for reparative therapy in endodontics and other areas. (fapesp.br)
Thus, the objective of the scientific initiation project is to study the possible role of the BMP2-Smad1/5/8 signaling pathway in differentiating of human pulp cells in osteogenic phenotype induced by CsinCPI-2. (fapesp.br)

Interestingly, no skeletal phenotype was observed in adolescent or adult female CCR3-deficient mice. (bvsalud.org)
Among juvenile CCR3-deficient mice, neither males nor females showed a skeletal phenotype, which indicated that bone modeling was not affected by the CCR3 deficiency. (bvsalud.org)
Single and double knock-out mice are being developed in order to determine the role of these proteins in skeletal modeling and remodeling. (yale.edu)
The function of pannexins in bone and cartilage is beginning to be uncovered, but more research is needed to determine the role of pannexins in bone development, adult bone mass and skeletal homeostasis. (biomedcentral.com)
We describe here the current knowledge on the role of connexins and pannexins on skeletal health and disease. (biomedcentral.com)
Our results showed an increase in the expression of Cbfa1 and OCN in fibroblasts and osteoblasts exposed to fluoride and suggested that the osteogenic function of fibroblasts induced by fluoride could play an important role in the development of extraperiosteal ossification during skeletal fluorosis. (fluoridealert.org)
MMPs play an important role in skeletal connective tissue degradation and remodeling. (allenpress.com)
A skeletal phenotype was not detectable by whole mount skeletal staining of knock out and age and sex matched control mice. (uconn.edu)
The canonical Wnt signaling pathway is critical for skeletal development and maintenance, but the precise roles of the individual Wnt co-receptors, Lrp5 and Lrp6, that enable Wnt signals to be transmitted in osteoblasts remain controversial. (vectorbiolabs.com)
The breaking research is revealing a number of roles for vitamin K reaching well beyond coagulation to not only long term cardiovascular and skeletal health, but that of the brain and nervous system, and also for insulin production and sensitivity, and genomic stability / cancer prevention. (vitamindwiki.com)

On days 7, 14 and 21 of osteogenic differentiation, the cultures were submitted to evaluations of MTT conversion in formazan, of alkaline phosphatase activity (AP), of collagen and mineralized matrix synthesis, evaluation of the number of cells per field and there was quantification of the gene transcripts for osterix, bone sialoprotein (BSP), osteonectin (ON) and osteocalcin (OC). (scielo.br)
During the fusion course of action a metaplastic shift appeared from the arch centra wherever cells inside the intermediate zone concerning osteoblasts and chon drocytes co transcribed col1a, col2a, runx2, osteocalcin make it clear and osteonectin, as visualized by ISH. (pkc-inhibitors.com)

The expression of the osteoblastic gene osteocalcin is reduced during embryonic life (days 18.5 and 19.5 post-coitum), whereas alkaline phosphatase and osteopontin levels are reduced early on but normalized or even increased by day 19.5 [ 18 ]. (biomedcentral.com)
I have also participated in the investigation of the regulation of bone formation by growth factor treatments and by genetically modifying mice leading the discovery of the periosteal anabolic effects of fibroblast growth factors in vivo, and characterizing the role of osteocalcin though osteocalcin gene knockout leading to a nature publication. (big-picture.net)
This pro-osteogenic effect was demonstrated by the increase in the alkaline phosphatase (ALP) activity, deposition of mineralized nodules and the gene expression of osteogenic markers such as bone morphogenetic protein 2 (BMP-2), runt-related transcription factor 2 (RUNX2), ALP, osteocalcin and bone sialoprotein (BSP). (fapesp.br)
Gene expression was studied using RNA from cultured cells by quantitative PCR method and showed changes in the expression pattern of other Dlx genes and multiple bone specific genes including expression of osteocalcin ( OCN ), bone sialoprotein ( BSP ), Collagen I ( Col1a1 ) and alkaline phosphatese. (uconn.edu)
Coordination of gene Tubastatin A HCl expression Tubastatin A HCl during osteoblast differentiation and bone remodeling represents an excellent model with which to test this novel physiological role of space junctional intercellular communication. (acancerjourney.info)
Synergistic induction of osteocalcin gene appearance. (acancerjourney.info)
Along with other BMPs, including BMP-2, -4 and -7, which are well known as potent osteoinductive growth factors, GDF-5 plays important roles in the development of bones, cartilage and tendons, as evidenced by the tendency for the gene and protein expression levels of GDF-5 to increase over time in these tissues. (ispub.com)

both dye transfer and appearance of osteocalcin (OC) and bone tissue sialoprotein (BSP) genes pivotal to bone matrix formation and calcification. (acancerjourney.info)
The overexpression of Rev-erbα in osteoblast precursors attenuated the expression of osteoblast marker genes including Runx2, alkaline phosphatase (ALP), bone sialoprotein (BSP), and osteocalcin (OC). (molcells.org)
The osteogenic differentiation of PDLSCs were assessed by ALP staining and Alizarin Red staining as well as the mRNA and protein levels of osteogenic marker genes osterix, osteocalcin, and alkaline phosphatase through qRT-PCR and western blot. (biomedcentral.com)

This posttranslational modification is found for instance in some coagulation factors (prothrombin, factor IX, etc.), in MGP, a protein implicated in tissue mineralization, and in osteocalcin, a bone-derived hormone affecting glucose metabolism. (umontreal.ca)
they not only effectively induced macrophage polarization to M2 phenotype but also promoted the migration, tube formation, and angiogenic differentiation of human umbilical vein endothelial cells (HUVECs) and facilitated the migration, osteo-differentiation, and extracellular matrix (ECM) mineralization of MC3T3-E1 cells. (biomedcentral.com)

It has also been suggested that Runx2 plays a cell proliferation regulatory role in cell cycle entry and exit in osteoblasts, as well as endothelial cells. (wikipedia.org)
In addition to being the master regulator of osteoblast differentiation, Runx2 has also been shown to play several roles in cell cycle regulation. (wikipedia.org)
It has also been proposed that decreasing levels of Runx2 leads to cell cycle exit for proliferating and differentiating osteoblasts, and that Runx2 plays a role in mediating the final stages of osteoblast via this mechanism. (wikipedia.org)
One study proposes that this phenotype arises partly due to the Runx2 dosage insufficiencies. (wikipedia.org)
Variants of Runx2 have been associated with the osteosarcoma phenotype. (wikipedia.org)
Current research suggests that this is partly due to the role of Runx2 in mitigating the cell cycle. (wikipedia.org)
Runx2 plays a role as a tumor suppressor of osteoblasts by halting cell cycle progression at G1. (wikipedia.org)

We have focused primarily on osteocalcin, a vitamin K-dependent protein synthesized only in bone. (yale.edu)
Xiao H, Chen J, Duan L, Li S. Role of emerging vitamin K‑dependent proteins: Growth arrest‑specific protein 6, Gla‑rich protein and periostin (Review). (voedingsgeneeskunde.nl)
We present here the first report of a functional role for any Ebf-family protein in kidney. (biodiversityhotspot.org)
Osteocalcin is a structural protein of the bone matrix, which is synthesized by osteoblasts and enters the bloodstream in the process of bone resorption. (dntb.gov.ua)
even in all healthy humans tested, a significant percentage of the extra-hepatic VKD proteins, which are activated by vitamin K2, e.g., osteocalcin and matrix Gla-protein, are only partly carboxylated and therefore inactive. (vitamindwiki.com)
This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor-associated factor (TRAF) 6, which indicated this protein may have a role in the regulation of cell apoptosis. (nih.gov)
To examine the role of OPN in vascular calcification, OPN mutant mice were crossed with matrix Gla protein (MGP) mutant mice. (silverchair.com)
P53 protein expression is widely inves tigated in leukemia and you can find numerous papers with regards to the vital roles of p53 during the pediatric leukemia. (micrornainhibitors.com)

A communicating intercellular network is necessary for the entire elaboration of the differentiated osteoblastic phenotype. (acancerjourney.info)

More recently, genetic and pharmacologic studies revealed the role of connexins, and in particular Cx43, in the differentiation and function of all bone types. (biomedcentral.com)
The number of cells per field, the AP activity, the collagen and mineralized matrix synthesis were higher in AT-MSC en differentiation, in relation to BM-MSC under the same conditions in all evaluated times. (scielo.br)
Specifically, the employment of BC-Ppy compositesdrives partial H9c2 differentiation toward a cardiomyocyte-like phenotype.The scaffolds increase the expression of functional cardiac markersin H9c2 cells, indicative of a higher differentiation efficiency,which is not observed with plain BC. (ibecbarcelona.eu)
In brief, lncRNA plays an important role in osteogenic differentiation of PDLSCs and our study will further investigate the effects of lncRNA SNHG1 in osteogenic differentiation of PDLSCs and explore the underlying mechanism. (biomedcentral.com)

Osteoarthritis: genetics and phenotypes in all their complexity. (voedingsgeneeskunde.nl)

We recently showed that adult male mice that lacked the C-C-chemokine receptor 3 (CCR3) exhibited disturbed bone remodeling, which resulted in a cortical bone phenotype of thin femoral cortical bone. (bvsalud.org)
To investigate whether the CCR3 bone phenotype was sex-related, we analyzed both young female and male mice, and adult females. (bvsalud.org)
In summary, adolescent and adult male mice that lacked CCR3 receptors exhibited a cortical phenotype that was not present in female mice, probably due to an estrogen protective mechanism. (bvsalud.org)

Current in vivo studies include analysis of the phenotype of several transgenic mice with targeted deletions of bone matrix proteins. (yale.edu)
In this paper, we review the current literature on both in vitro and in vivo studies on the role of the immune system in fracture repair, the use of MSCs in the enhancement of fracture healing, and interactions between MSCs and immune cells. (hindawi.com)

We employed cell proliferation analyses such as CCK8 and BrdU assays to determine the proliferation-promoting role of TSC-EVs on MSCs. (biomedcentral.com)

We are currently actively involved in characterizing the circulating forms of osteocalcin and defining the factors that influence its synthesis and secretion. (yale.edu)
We are (1) investigating the source and regulation of osteocalcin production, and (2) identifying regulators of osteocalcin synthesis and secretion in vivo and in vitro. (yale.edu)
Osteocalcin promotes pancreatic β-cell proliferation and insulin secretion, and also affects the insulin sensitivity of peripheral tissues. (dntb.gov.ua)

2003). A previous in-vitro study on the role of GDF-5 in periodontal ligament cells has shown that exogenous GDF-5 promotes cell proliferation while suppressing the activity of alkaline phosphatase (ALP), which is closely related to tissue calcification and which is secreted during the formation of tissue calcifications. (ispub.com)

Undifferentiated mesenchymal stem cells are pluripotent cells that can differentiate into various phenotypes and are a source of osteogenic Cells 1,2 . (bvsalud.org)
To date, various studies have reported that cells isolated from the dental pulp of various animals can be induced to differentiate into cells of the odontoblastic phenotype by means of various chemicals, growth factors and signaling molecules both in vitro and in vivo ( reviewed in Nakashima and Akamine, 2005). (ispub.com)

Several in vitro scientific studies have demonstrated that chon drocytes related with calcifying cartilage can acquire properties of osteoblasts and therefore are able to change their phenotype from a primarily cartilage www.selleckchem.com/products/Romidepsin-FK228.html synthesizing cell type to a bone synthesizing cell style. (pkc-inhibitors.com)
Structure, formation and role of cartilage canals in the developing bone. (heightquest.com)

Zhou R, Guo Q, Xiao Y, Guo Q, Huang Y, Li C, Luo X. Endocrine role of bone in the regulation of energy metabolism. (dntb.gov.ua)

Likewise, calvarial cells doubly deficient for Lrp5 and Lrp6 failed to form osteoblasts when cultured in osteogenic media, but instead attained a chondrocyte-like phenotype. (vectorbiolabs.com)

To that end we are investigating the relationships between vitamin K status, calcium homeostasis and biosynthesis of functional osteocalcin. (yale.edu)

I was a part of the research team that discovered roles of RANK, RANKL and osteoprotegerin (OPG) in the regulation of bone resorption and in the discovery of denosumab which targets this regulatory pathway. (big-picture.net)
Our results suggest that in response to stretch, MMP-2 responds rapidly by inhibiting conversion of a MMP-2 to the active form, while a slower up-regulation of MMP-9 may play a role in the long-term remodeling of extracellular matrix in response to continuous mechanical loading. (allenpress.com)

Diversity of Gut Microbiota Affecting Serum Level of Undercarboxylated Osteocalcin in Patients with Crohn's Disease. (voedingsgeneeskunde.nl)
It is shown that the level of osteocalcin in the blood serum is significantly reduced compared to healthy controls, both in patients with type 1 diabetes mellitus and, especially, in type 2 diabetes mellitus. (dntb.gov.ua)

Expanded natural killer cells killed both allogeneic and autologous primary myeloma cells avidly via a perforin-mediated mechanism in which the activating receptor NKG2D, natural cytotoxicity receptors, and DNAX-accessory molecule-1 played a central role. (haematologica.org)
The nuclear receptors Rev-erbs, comprising Rev-erbα and Rev-erbβ, play a key role as transcriptional regulators of the circadian clock. (molcells.org)

Among bone hormones, osteocalcin plays an important role as a coordinator of bone modeling processes, energy homeostasis, metabolism of glucose, lipids and minerals. (dntb.gov.ua)

This project aimed to understand role of Dlx3 in bone formation and metabolism. (uconn.edu)
In this review, we summarize current knowledge about vitamin D metabolism in general, its role in diabetes mellitus (mainly type 2) and diabetic complications (mainly diabetic kidney disease), and potential therapeutic perspectives including vitamin D signalling as a druggable target. (karger.com)
The Effect of Genistein Supplementation on Vitamin D Levels and Bone Turnover Markers during the Summer in Healthy Postmenopausal Women: Role of Genotypes of Isoflavone Metabolism. (cdc.gov)

The level of osteocalcin in the serum is used as a specific marker of bone formation. (dntb.gov.ua)

After bone fractures mesenchymal stromal cells (MSCs) take on a key role in the in vivo bone repair process 1 . (nature.com)
This study aimed to clarify the role of mesenchymal stem cells (MSCs) as a component of the cancer microenvironment. (cancerindex.org)

These findings are expected to improve our knowledge of the role of GDF-5 in dental tissue development and to provide information that may be useful in the application of GDF-5 as a molecular manipulator in dental tissue regeneration. (ispub.com)
However, they are easy to obtain from human adult tissues without ethical issues and play an essential role in tissue repair and organ homeostasis [ 1 , 2 ]. (biomedcentral.com)

In these studies, we used Cre-loxP recombination, in which Cre-expression is driven by the human osteocalcin promoter, to determine the individual contributions of Lrp5 and Lrp6 in postnatal bone acquisition and osteoblast function. (vectorbiolabs.com)

However, whether RESV promotes function of senescent stem cells in alleviating age-related phenotypes remains largely unknown. (thno.org)

However, it remains unknown whether this phenotype would be present during bone modeling, or it affects female mice. (bvsalud.org)
Of particular note was my contribution to an article in Nature describing the phenotype of osteocalcin knockout mice. (big-picture.net)
We initially investigated a role for Ebf1 in kidney maturation during exploration of the mechanism underlying the bone phenotype of Ebf1-deficeint mice. (biodiversityhotspot.org)
Results Ebf1-deficient mice have decreased GFR While examining Ebf1s role in osteoblast function (6) we identified an incongruity between the quality of bone, and the level of circulating osteocalcin (Ocn). (biodiversityhotspot.org)
Following that, this work aimed to conduct a case-control study of thoracic, zygomatic and masticatory muscular morphology to propose an association between musculoskeletal phenotypes and deficiency of Cebpb, using a sample of Cebpb -/- , Cebpb +/- and Cebpb +/+ adult mice. (biomedcentral.com)
Although Cebpb +/- mice did not show the phenotypes above (p = 0.357), a larger mass percentage of temporal muscles over masseter muscles was seen in Cebpb +/- littermates (p = 0.012). (biomedcentral.com)

The underlying molecular mechanism can be partly attributed to the immunomodulation of the Cs/Gel/PHA/PBT hydrogels as shown via transcriptome sequencing analysis, and the PI3K/Akt signaling axis plays an important role in regulating macrophage M2 polarization. (biomedcentral.com)

This, in turn would increase the levels of osteocalcin which is responsible for bone formation. (umc.edu)

A recent study supports the pivotal role of trophoblast exosomes in that extracellular vesicles (EVs) derived from TSCs promote fertility by regulating endometrial cell receptivity [ 17 ]. (biomedcentral.com)

The myocardial nicheplays a critical role in directing the function and fate of cardiomyocytes;therefore, engineering a biomimetic scaffold holds excellent promise.We produced an electroconductive cardiac patch of bacterial nanocellulose(BC) with polypyrrole nanoparticles (Ppy NPs) to mimic the naturalmyocardial microenvironment. (ibecbarcelona.eu)

Due to the limited availability of genetic models in exploring the function of Rev-erbβ, the role of Rev-erbα in mammalian circadian and metabolic physiologies is comparatively well-known. (molcells.org)
The Genetic Predisposition Score of Seven Obesity-Related Single Nucleotide Polymorphisms Is Associated with Better Metabolic Outcomes after Roux-en-Y Gastric Bypass. (cdc.gov)

Referenced article focusing on the relationship between phenotype and genotype. (cancerindex.org)

The inverse association of glycemia with the level of osteocalcin was revealed. (dntb.gov.ua)

Hence PKC enzymes play important roles in several signal transduction cascades. (pkc-inhibitors.com)

In addition to their role as membrane channels, connexins have been shown to interact with intracellular structural and signalling molecules [ 9 ], adding yet another layer of complexity to their function. (biomedcentral.com)

To that end, we have developed site specific antibodies and are investigating the circulating forms of osteocalcin through the use of these monoclonal antibodies. (yale.edu)

2 The cooperative effects of collagenases, gelatinases, and stromelysins-in combination with mechanical forces-suggest a role in MMP-dependent degradation of the extracellular matrix as an important determinant of implant failure due to excess occlusal forces. (allenpress.com)

Less is known about the role of Cx43 in other cell types during bone development. (biomedcentral.com)

Another less likely possibility is that systemic inflammation may be beneficial and may play a protective role by enhancing defence and repair mechanisms, but this seems unlikely. (ersjournals.com)

Anatomy11

Diseases9

Chemicals and Drugs30

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes13

Information Science2

Serum osteocalcin2

Osteogenic phenotype3

Skeletal10

Osteonectin2

Gene7

Genes3

Mineralization2

Runx27

Protein8

Osteoblastic1

Differentiation4

Osteoarthritis1

CCR33

Vivo studies2

Assays1

Secretion3

Alkaline1

Differentiate2

Cartilage2

Endocrine1

Osteoblasts1

Functional1

Regulation2

Patients2

Receptors2

Glucose1

Metabolism3

Marker1

MSCs2

Tissue2

Expression1

Cells1

Mice6

Important role1

Formation1

Study1

Cardiac1

Metabolic2

Article1

Level1

Enzymes1

Function1

Specific1

Extracellular1

Development1

Inflammation1