Role myosin light chains. Medical search. Frequent questions

Myosin light chain kinase (MLCK) inhibitors are one of the few peptides that can cross the plasma membrane relatively quickly. (wikipedia.org)
Myosin light-chain kinase Myosin-light-chain phosphatase Myosin+Light+Chains at the U.S. National Library of Medicine Medical Subject Headings (MeSH) Zhang Y (June 2010). (wikipedia.org)
Key role of myosin light chain (MLC) kinase-mediated MLC2a phosphorylation in the alpha 1-adrenergic positive inotropic effect in human atrium. (nih.gov)
It is well known that smooth-muscle contraction is regulated by the cytosolic Ca2+ concentration and by the Ca2+ sensitivity of myofilaments: the former activates myosin light-chain kinase, and the latter is achieved partly by the inhibition of myosin phosphatase. (jefferson.edu)
The phospho-myosin light chain (p-MLC), phospho-myosin light chain kinase (p-MLCK), and phospho-myosin phosphatase target subunit 1 (p-MYPT1) levels in the bladder were examined using Western blot. (hindawi.com)
The function of striated or smooth muscle is regulated by the balance of myosin light chain kinase (MLCK) and MLCP activity. (hindawi.com)
Repeated passage cells treated with TSA and AZA increased expression of muscarinic receptor 3, myosin light chain kinase 2, and showed an increased response to carbachol stimulation. (queensu.ca)
Myosin-Light-Chain Kinase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ouhsc.edu)
This graph shows the total number of publications written about "Myosin-Light-Chain Kinase" by people in this website by year, and whether "Myosin-Light-Chain Kinase" was a major or minor topic of these publications. (ouhsc.edu)
Below are the most recent publications written about "Myosin-Light-Chain Kinase" by people in Profiles. (ouhsc.edu)
Regulation of LPA-promoted myofibroblast contraction: role of Rho, myosin light chain kinase, and myosin light chain phosphatase. (ouhsc.edu)
Scholars@Duke publication: The role of protein kinase C in modulation of aqueous humor outflow facility. (duke.edu)
Based on knowledge regarding the role of protein kinase C (PKC) in vascular biology, we sought to understand the contribution of the PKC pathway towards outflow function by studying the modulation of contractile and morphological characteristics of TM and SC cells. (duke.edu)
In view of the ability of calmodulin to bind vasoactive intestinal peptide (VIP) and growth-hormone-releasing factor (GRF) with high affinity, the effects of these neuropeptides on a model calmodulin-dependent enzyme, myosin light-chain kinase (MLCK), were studied. (elsevierpure.com)
In the literature, many biological molecules inside VSMC, such as platelet-derived growth factor-BB (PDGF-BB), platelet-derived growth factor receptor, phosphorylated myosin light-chain kinase (pMLCK), have been found to be influenced by the mechanical stimulation. (uwaterloo.ca)
In addition, they play important roles in gene transcription (via activation of mitogen activated protein (MAP) kinase pathways and, in higher eukaryotes, the transcription factor NFκB), generation of reactive oxygen species, apoptosis, and cell-cycle progression. (biomedcentral.com)
I investigated the roles of RhoA-kinase and myosin II in semaphorin-3A-induced growth cone collapse and axon retraction. (biologists.com)
Formation of axonal F-actin bundles was independent of myosin II, but partially required RhoA-kinase activity. (biologists.com)
1988). Kinases responsible for the phosphorylation include myosin light chain kinase (MLCK), ROCK kinase, citron kinase, myotonic dystrophy kinase-related CDC42-binding protein kinase, and Zipper-interacting protein (ZIP) kinase. (reactome.org)
PubMed]Kawano Y, Fukata Y, Oshiro D, Amano Meters, Nakamura Testosterone levels, Ito Meters, Matsumura Y, Inagaki Meters, Kaibuchi T. Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo. (opioid-receptors.com)
Identification of the endogenous easy muscle myosin phosphatase-associated kinase. (opioid-receptors.com)
PubMed]Murata-Hori M, Fukuta Y, Ueda K, Iwasaki T, Hosoya H. HeLa Squat kinase induces diphosphorylation of myosin II regulatory light chain and reorganization of actin filaments in nonmuscle cells. (opioid-receptors.com)
PubMed]Murata-Hori M, Suizu F, Iwasaki T, Kikuchi A, Hosoya H. Squat kinase identified as a novel myosin regulatory light chain kinase in HeLa cells. (opioid-receptors.com)
RhoA/Rho kinase(ROCK) inhibitor that plays an important role in mediating vasoconstriction and vascular remodeling in the pathogenesis of PH. (aksci.com)
Rho kinase inhibitor HA-1077 prevents Rho-mediated myosin phosphatase inhibition in smooth muscle cells. (aksci.com)
We have investigated the role of the actin cytoskeleton in noradrenaline (NA)-induced and endothelin (ET)-induced activation of the calcium-dependent nonreceptor tyrosine kinase PYK2 and subsequent phosphorylation of paxillin in rat small mesenteric arteries. (manchester.ac.uk)
The mechanical force that activates Piezo1 arises from Myosin II phosphorylation by Myosin Light Chain Kinase. (nature.com)
PKA phosphorylates myosin light chain kinase, inhibiting its activity. (fullfigurednews.com)
The phosphorylation of myosin light chain kinase decreases the sensitivity of the contractile machinery to calcium. (fullfigurednews.com)
In this study, we analyzed bile canaliculi dynamics, Rho kinase (ROCK)/myosin light chain kinase (MLCK) pathway implication, efflux inhibition of taurocholate [a predominant bile salt export pump (BSEP) substrate], and expression of the major canalicular and basolateral bile acid transporters. (hal.science)

The level of myosin light chain (MLC) phosphorylation is an important determinant of smooth muscle contraction. (hindawi.com)
The phosphorylation of smooth muscle regulatory light chain by MLCK is the basic requirement for smooth muscle contraction and hollow organ physiological activities [ 10 ]. (hindawi.com)
Work done using smooth muscle myosin and mammalian non-muscle myosin have demonstrated that phosphorylation of the RLC at conserved Serine and Threonine sites ( Figure 1B , Serine-19 and Threonine-18) activates myosin motor activity, enhances the affinity of myosin for actin, and promotes myosin filament assembly ( Heissler and Sellers, 2016 ). (elifesciences.org)
However, it has not been biochemically demonstrated that Drosophila myosin motor activity and filament assembly is regulated by RLC phosphorylation or whether the extent of activation is similar to that of mammalian systems. (elifesciences.org)
We investigated the involvement of PKC in regulation of myosin light chain (MLC) phosphorylation, formation of actin stress fibers and integrin-ECM adhesions (focal adhesions) in human TM and SC cells and correlated these changes with aqueous outflow facility measured in an enucleated porcine whole eye perfusion model. (duke.edu)
These results suggest that PKC might play an important role in modulation of aqueous outflow facility by regulating MLC phosphorylation and thereby, the morphological and cytoskeletal characteristics of TM and SC cells. (duke.edu)
Myosin II activity is stimulated by phosphorylation of MRLC. (reactome.org)
Apoptotic membrane blebbing is usually regulated by myosin light chain phosphorylation. (opioid-receptors.com)
Treatment of arteries with cytochalasin D disrupted stress fibers and inhibited NA-induced and ET-induced force in a myosin light chain 20 phosphorylation independent and reversible manner. (manchester.ac.uk)
This leads to reduced phosphorylation of myosin, a protein that plays a crucial role in muscle contraction. (fullfigurednews.com)
Here, we characterize the regulatory roles of serine-375 (S375), one of the major phosphorylation sites of E2F1. (bvsalud.org)
These effects were mediated via the down-regulation of caspase-3, ROCK1 (Rho-associated kinase1) activation and myosin light chain (MLC, Ser-19) phosphorylation. (ijbs.com)

The cellular activity of myosin light chain phosphatase (MLCP) determines the agonist-induced force development in smooth muscle. (jefferson.edu)
Myosin light chain phosphatase (MLCP) activity is associated with mammalian bladder function, and it is speculated that a change in MLC may play an important role in the nervous and myogenic control of the bladder [ 7 ]. (hindawi.com)
The dephosphorylation activity of Rabbit Polyclonal to HUNK myosin phosphatase immunoprecipitated from the apoptotic cells was lower than that from the nonapoptotic control cells. (opioid-receptors.com)
PMC free of charge content] [PubMed]Mabuchi T, Gong BJ, Langsetmo T, Ito Meters, Nakano T, Tao T. Isoforms of the small non-catalytic subunit of easy muscle myosin light chain phosphatase. (opioid-receptors.com)
PMC free article] [PubMed]Mizuno T, Tsutsui K, Nishida Y. myosin phosphatase and its role in dorsal closure. (opioid-receptors.com)
ROCK induces vasoconstriction by phosphorylating the myosin-binding subunit of myosin light chain (MLC) phosphatase, thus decreasing MLC phosphatase activity and enhancing vascular smooth muscle contraction. (aksci.com)

It is thought that MLCK phosphorylates endothelial myosin, leading to cell contraction. (wikipedia.org)
Cotreatment with ROCK inhibitor Y-27632 [4-(1-aminoethyl)-N-(4-pyridyl) cyclohexanecarboxamide dihydrochloride] and MLCK activator calmodulin reduced bile canaliculi constriction and dilatation, respectively, confirming the role of these pathways in drug-induced intrahepatic cholestasis. (hal.science)

Structurally, myosin light chains belong to the EF-hand family, a large family of Ca2+- binding proteins. (wikipedia.org)
Among them are, for example, MYL6B, MYLIP, MYLK, and MYLK2, Several diseases have been associated with mutations in the genes encoding for myosin light chain proteins. (wikipedia.org)
The second major area of investigation is to understand the role of cytoskeleton proteins, desmin and vimentin in smooth muscle contraction and mitochondrial respiration. (jefferson.edu)
The contractile ring is made up of structural proteins, including actin filaments and myosin II filaments. (jove.com)
The rationale of this organization may be related to important roles of these proteins as "exchange hubs" for the signaling proteins for their migration from the subcortical cytosol to the membrane. (frontiersin.org)
It has been recently reported that motor proteins like myosin Va play important role in transcytosis of nNOS. (frontiersin.org)
Evidence has suggested the role of motor proteins like myosin Va in transposition of nNOS within the nerve terminals to the membranes to facilitate nitrergic neurotransmission ( 11 , 12 ). (frontiersin.org)
Based on the structure of Cdc4p, possible models for interallelic complementation including interactions with partner proteins and the formation of a myosin complex with Cdc4p fulfilling the role of both an essential and regulatory light chain are proposed. (ubc.ca)
The long tail region interacts with other proteins, including the tail regions of other myosin proteins. (medlineplus.gov)
A family of proteins that play a role as cofactors in the process of CLATHRIN recycling in cells. (lookformedical.com)

An enzyme that phosphorylates myosin light chains in the presence of ATP to yield myosin-light chain phosphate and ADP, and requires calcium and CALMODULIN. (ouhsc.edu)

A myosin light chain is a light chain (small polypeptide subunit) of myosin. (wikipedia.org)

I report that semaphorin 3A activates myosin II in growth cones and axons. (biologists.com)

Collectively, these observations suggest that guidance cues cause axon retraction through the coordinated activation of myosin II and the formation of intra-axonal F-actin bundles for myosin-II-based force generation. (biologists.com)

LIMCH1 interacted with NM-IIA, but not NM-IIB, independent of the inhibition of myosin ATPase activity with blebbistatin. (nih.gov)

Deduce the structural and functional l roles of My-BP-C in muscle contraction, collaboration with University of Massachusetts Medical School. (iit.edu)
Deduce the structural and functional and structural roles of nebulin in muscle contraction and their role in nemaline myopathy, collaboration with University of Arizona/NIH. (iit.edu)
Both calcium- and Ca2+ sensitization- mediated contraction plays role in G-protein signaling during smooth-muscle contraction. (jefferson.edu)
The long-term goals of this project are to elucidate the role of GPCR mediated signaling in smooth muscle contraction and proliferation under pathophysiological conditions. (jefferson.edu)
Mitochondria and smooth muscle contraction: Role of desmin cytoskeleton protein. (jefferson.edu)
One mechanism of contraction involves myosin motor activity. (jove.com)
Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. (elifesciences.org)
Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos. (elifesciences.org)
The bipolar myosin filaments promote the sliding of antiparallel actin filaments relative to one another resulting in contraction of an actin network. (elifesciences.org)
The enzyme plays a central role in the regulation of smooth muscle contraction. (ouhsc.edu)
Nonmuscle myosin II (NMM2) is an actin-based motor protein that plays a crucial role in a variety of cellular processes, including smooth muscle contraction, cell migration, polarity formation, and cytokinesis. (reactome.org)
"It was expected answers would describe in detail the role of troponin, tropomyosin and calmodulin in mediating muscle contraction" , the examiners rejoined. (derangedphysiology.com)

Reorganization of the actin cytoskeleton plays crucial roles in many cellular functions, including morphology, motility, and adhesion. (biomedcentral.com)
Ohanian, V, Gatfield, K & Ohanian, J 2005, ' Role of the actin cytoskeleton in G-protein-coupled receptor activation of PYK2 and paxillin in vascular smooth muscle ', Hypertension , vol. 46, no. 1, pp. 93-99. (manchester.ac.uk)

These results suggest that down-regulation of MYPT1 may play a role in promoting hyperphosphorylation of myosin II by inhibiting the dephosphorylation of myosin II during apoptosis. (opioid-receptors.com)

Nonmuscle myosin II (NM-II) is an important motor protein involved in cell migration. (nih.gov)

Differential regulation of the atrial isoforms of the myosin light chains during striated muscle development. (nih.gov)
Collectively, our data suggest that LIMCH1 plays a positive role in regulation of NM-II activity through effects on MRLC during cell migration. (nih.gov)
These findings put some insights into the unique neuroprotective properties of Rg1 associated with the regulation of myosin IIA-actin cytoskeletal structure under oxidative stress and provide experimental evidence for Rg1 in CNS diseases. (ijbs.com)

Nevertheless, the association of MLCs with the neck region of MHCs is necessary for the assembly of the macromolecular complexes that result in the functional motor protein, myosin. (wikipedia.org)
The Schizosaccharomyces pombe Cdc4 protein is required for the formation and function of the contractile ring, presumably acting as a myosin light chain. (ubc.ca)
The MYH9 gene provides instructions for making a protein called myosin-9. (medlineplus.gov)
This protein is one part (subunit) of the myosin IIA protein. (medlineplus.gov)
Each type of myosin II protein consists of two heavy chains and four light chains. (medlineplus.gov)
Most of the mutations that cause this condition change single protein building blocks (amino acids) in the myosin-9 protein. (medlineplus.gov)
Mutations that are located near the head of the myosin protein tend to lead to a more severe disorder than mutations that are located toward the tail of the protein. (medlineplus.gov)
A nonfunctional myosin-9 protein cannot properly interact with other subunits to form myosin IIA. (medlineplus.gov)
NMM2 consists of two myosin heavy chains encoded by MYH9, MYH10, MYH14 (NMHC-IIA, B and C) or MYH11, two copies of MYL6 essential light chain protein, and two regulatory light chains (MRLCs), MYL9 and MYL12B. (reactome.org)
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
the tadpoles showed reduced expression of cα(E)-catenin , small muscle protein, dystrophin , and myosin light chain genes. (biomedcentral.com)

Study the deleterious effect of mutations in the myosin light chains in cardiomyopathies, collaboration with University of Miami Medical School. (iit.edu)

In the embryonic period, myosin regulatory light chain (MYL2) plays a pivotal role in the development and function of the heart. (shsmu.edu.cn)

The work was completed in Maastricht and provided the basis for a thesis on the function of the promoter of the atrial Myosin Light Chain gene. (umcutrecht.nl)

Functional divergence of human cytoplasmic myosin II: kinetic characterization of the non-muscle IIA isoform. (nih.gov)
These data provide support for a functional role of VIP and GRF binding by calmodulin. (elsevierpure.com)
Platelets, which only express myosin IIA, are most affected by a lack of functional myosin-9, accounting for the thrombocytopenia seen in all individuals with MYH9 -related disorder. (medlineplus.gov)

Depletion of LIMCH1 attenuated myosin regulatory light chain (MRLC) diphosphorylation in HeLa cells, which was restored by reexpression of small interfering RNA-resistant LIMCH1. (nih.gov)

The proposed mechanism state that "Myl9/12-containing net-like structures are created in inflammatory vessels, which play an important role as a platform for recruitment of CD69-expressing leukocytes into inflammatory tissues. (wikipedia.org)
Although the role of axon retraction during development of the nervous system is well appreciated, the mechanism of guidance-cue-induced retraction is not fully elucidated. (biologists.com)
In this article, we will delve into the mechanism of salbutamol and its role in treating bronchospasm. (fullfigurednews.com)
Accordingly, the neuroprotective mechanism of Rg1 is related to the activity that inhibits myosin IIA-actin interaction and the caspase-3/ROCK1/MLC signaling pathway. (ijbs.com)

Here, myosin filaments move toward the plus end of adjacent antiparallel actin filaments. (jove.com)

Myosin is a hexamer composed of two myosin heavy chains, two regulatory light chains (RLCs), and two essential light chains (ELCs) ( Figure 1A ). (elifesciences.org)

MLCs are structurally and functionally distinct from myosin heavy chains (MHCs). (wikipedia.org)

The motor domain at the N-terminus of the myosin heavy chain binds actin filaments in an ATP-dependent manner. (elifesciences.org)
The light chains bind to the central neck domain of the myosin heavy chain and have structural and regulatory functions ( Heissler and Sellers, 2014 ). (elifesciences.org)
The C-terminal tail of the myosin heavy chain associates with the tails of other myosin heavy chains and promotes the assembly into bipolar filaments. (elifesciences.org)
A ) Domain organization of the myosin heavy chain and myosin fragments used to study the biochemical properties of myosin. (elifesciences.org)
The myosin motor domain, the light chain binding neck and the tail domain of the heavy chain are indicated. (elifesciences.org)
The heavy chain subunits of clathrin. (lookformedical.com)

Any epigenetic role in regulating gene expression and the contractile phenotype of ISMC is unknown. (queensu.ca)
MYL2 gene mutation impacts the structure and function of myosin, thereby leading to the occurrence and progression of hypertrophic and dilated cardiomyopathy as well as the following chronic heart failure. (shsmu.edu.cn)
The role of MRCKα was explored, either by expression of dominant negative mutant or by gene silencing using the specific RNAi and CRISPR-cas9 strategy in cell models. (sorbonne-universite.fr)

RhoA activity results in the assembly of myosin II and anti-parallel actin filaments into the structure of the contractile ring. (jove.com)
Here, combining the biochemical analysis of enzymatic and motile properties for purified myosin mutants with in vivo measurements of apical constriction for the same mutants, we show that in vivo constriction rate scales with myosin motor activity. (elifesciences.org)
The defect in the myosin motor activity in these mutants is evident in developing Drosophila embryos where tissue recoil following laser ablation is decreased compared to wild-type tissue. (elifesciences.org)
Myosin II activity is required for axon retraction but not growth cone collapse. (biologists.com)
Furthermore, inhibiting myosin II activity with blebbistatin partly blocked the neuroprotective effects of Rg1. (ijbs.com)

They play a role in the intracellular transport of molecules contained within membrane vesicles. (lookformedical.com)

Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. (lookformedical.com)

One study, published in 2012, found that valvular myosin 'LC1', in the hearts of three patients with valvular heart diseases, had structures similar to those of valvular myosin of people who were in their early stages of DCMP and HCMP. (wikipedia.org)

Myosin II interacts with F-actin to generate contractile forces that result in axon retraction. (biologists.com)
how can myosin II drive axon retraction if the major source of the required substratum for force generation, growth cone F-actin, has been depleted? (biologists.com)
The Rg1 treatment also abolished H 2 O 2 -induced morphological changes, including cell rounding, membrane blebbing, neurite retraction and nuclei condensation, which were generated by myosin IIA-actin interaction. (ijbs.com)

Voltage-dependent anion channel (VDAC) located at the mitochondrial outer membrane play a crucial role in regulating the metabolic and energetic functions of mitochondria and are primarily responsible for the ATP/ADP flux across the outer mitochondrial membrane. (jefferson.edu)
Compliance mismatch is suggested to play an important role in IH because of mechanotransduction. (uwaterloo.ca)
In this thesis, the roles crosslinking density and interlayer adhesion play in compliance and burst pressure of PVA vascular grafts were studied. (uwaterloo.ca)
ENDOSOMES play a central role in endocytosis. (lookformedical.com)
A family of high molecular weight GTP phosphohydrolases that play a direct role in vesicle transport. (lookformedical.com)

Firstly, FITC-filled SiNPs, were engineered with two different amounts of Hc-TZ (trastuzumab half-chain) per single nanoparticle (1:2 and 1:8, SiNPs to Hc-TZ ratio), which was 99m Tc-radiolabeled at histidine residues for ex vivo and in vivo biodistribution evaluations. (dovepress.com)

Anatomy32

Organisms12

Diseases3

Chemicals and Drugs127

Analytical, Diagnostic and Therapeutic Techniques and Equipment22

Phenomena and Processes57

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science3

Kinase30

Phosphorylation12

Phosphatase6

MLCK2

Proteins10

Phosphorylates1

Subunit of myosin1

Activates1

Activation of myosin1

Inhibition of myosin1

Contraction12

Cytoskeleton2

Dephosphorylation1

Nonmuscle1

Regulation3

Protein11

Mutations1

Embryonic1

Atrial1

Functional3

Diphosphorylation1

Mechanism4

Antiparallel actin filaments1

Regulatory light chains1

Distinct1

Heavy chain6

Gene3

Activity5

Intracellular1

Consists1

Diseases1

Retraction3

Play5

Vivo1