Role cell migration focal adhesions. Medical search. Frequent questions

Free glycosaminoglycans (GAGs) and proteoglycan- (PG-) containing GAGs, key effectors of cell surface, pericellular and extracellular microenvironments, perform multiple functions in cancer by virtue of their coded structure and their ability to interact with both ligands and receptors that regulate cancer growth [ 1 - 4 ]. (hindawi.com)
Specifically, these extracellular matrix (ECM) components critically modulate the tumor cell "motile phenotype" affecting their adhesive/migratory abilities which are directly correlated to the metastatic cascade [ 5 , 6 ]. (hindawi.com)
GAGs bound into PGs are located to the extracellular matrix, basal membrane, and cell surface [ 7 ]. (hindawi.com)
Cell adhesion (the physical interaction of a cell with another cell or with the extracellular matrix (ECM)) is essential for cell migration and tissue integrity. (cellmigration.org)
Focal adhesions physically link the cell cytoskeleton to the extracellular matrix, but it remains challenging to image single focal adhesions directly. (mysciencework.com)
Talin activates integrins and flexibly connects integrins to the actomyosin cytoskeleton, thereby serving as a 'molecular clutch' that transmits forces to the extracellular matrix to drive cell migration. (hal.science)
Significant reductions in the phosphorylation of signaling molecules focal adhesion kinase, extracellular signal-regulated kinases 1 and 2, and v-Akt murine thymoma viral oncogene were observed in male MIPβ1KO islets when compared to controls. (oncotarget.com)
β1 integrin is the major β integrin subunit in pancreatic beta-cells and forms heterodimers with 12 α integrin subunits, which interact with components of the extracellular matrix (ECM) to control intracellular signaling via the focal adhesion kinase (FAK) and extracellular signal related kinase 1 and 2 (ERK1/2) pathways [ 4 ]. (oncotarget.com)
Focal adhesions are multi-protein complexes that connect the cell's cytoskeleton to the extracellular matrix, allowing the cell to sense and respond to mechanical cues from its environment. (shubhasis-haldar-cmt.com)
The cross-talk between dynamic microtubules and integrin-based adhesions to the extracellular matrix plays a crucial role in cell polarity and migration. (kent.ac.uk)
Although physiological stimuli for controlling FAK nuclear localization have not been completely characterized, FAK shuttles from focal adhesions to the nucleus to directly convey extracellular signals. (molcells.org)
Focal adhesions connect extracellular matrix (ECM) and F-actin cytoskeleton through transmembrane protein integrins [3]-[6]. (technologybooksindustrialprojectreports.com)
Single cell mesenchymal-type movement is characterized by an elongated cellular shape and again requires extracellular proteolysis and integrin engagement. (biomedcentral.com)
For example, matrix metalloproteinases (MMPs), such as MMP-2 and −9, have been shown to be typical NF-κB target proteins that are responsible for extracellular matrix breakdown and cell invasion ( 7 ). (spandidos-publications.com)
These cell adhesion molecules (CAM) will mediate the contact between two cells or between cells and the extracellular matrix, an essential process to the success of the implant. (bvsalud.org)
The main reported mechanisms involve cell adhesion molecules (CAMs) and extracellular matrix components. (bvsalud.org)
Some of these molecules are glycoproteins expressed on the cell surface, socalled cell adhesion molecules (CAM), which mediate either the contact between two cells or the contact among cells and the extracellular matrix, being therefore of fundamental importance for adhesion 3 . (bvsalud.org)
To occur the adhesion of CAMs to the biomaterial, it is necessary their interaction with some components of extracellular matrix, such as proteoglycans, collagen and proteins 23 . (bvsalud.org)

Although focal adhesions are similar, in terms of molecular composition, to nascent adhesions and focal complexes, several adhesion proteins appear to change as focal adhesions evolve in response to tension, e.g., zyxin and vinculin . (cellmigration.org)
Podosomes are structurally divided into a core, which mainly contains proteins involved in actin polymerization (such as WASP, the Arp2/3 complex and cortactin ), and a surrounding ring populated by integrin receptors and adhesion proteins (for example, paxillin and focal adhesion kinase (FAK/Pyk2) ) [15] . (cellmigration.org)
Kank proteins accumulate at the lateral border of FAs, which we term the FA belt, and in central sliding adhesions, where they directly bind the talin rod domain through the Kank amino-terminal (KN) motif and induce talin and integrin activation. (hal.science)
In addition, Kank proteins diminish the talin-actomyosin linkage, which curbs force transmission across integrins, leading to reduced integrin-ligand bond strength, slippage between integrin and ligand, central adhesion formation and sliding, and reduced cell migration speed. (hal.science)
Our data identify Kank proteins as talin activators that decrease the grip between the integrin-talin complex and actomyosin to regulate cell migration velocity. (hal.science)
Cell migration is controlled by a force-driven cellular brake system called focal adhesion (FA), a multiprotein assembly of almost 200 proteins. (shubhasis-haldar-cmt.com)
These FA proteins interact mechanically and control the cell migration through traction force. (shubhasis-haldar-cmt.com)
Mechanosensitive proteins play a crucial role in focal adhesion-mediated neurodegeneration, a process that involves the breakdown of nerve cells in the central nervous system due to abnormal mechanical forces acting on the cells. (shubhasis-haldar-cmt.com)
Understanding the role of mechanosensitive proteins in focal adhesion-mediated neurodegeneration is critical for developing targeted therapeutic interventions. (shubhasis-haldar-cmt.com)
Business and dynamics of focal adhesion proteins have been well characterized in cells grown on two-dimensional (2D) cell culture surfaces. (technologybooksindustrialprojectreports.com)
Using sFCS we found higher percentage of slow diffusing proteins at these focal spots suggesting assembling/disassembling processes. (technologybooksindustrialprojectreports.com)
Western blot assessment confirmed that myc tagged human SOD1 proteins were induced by doxycycline in these cell lines. (hormonespathway.com)
The mechanism of action of HLCL-61 hydrochloride involves the inhibition of specific enzymes and proteins that play a crucial role in cell function and signal transduction. (thebiotek.com)
Rho GTPases represent a family of small GTP-binding proteins involved in cell cytoskeleton organization, migration, transcription, and proliferation. (biomedcentral.com)
Tensin is a gene family with four members (tensin1, tensin2, tensin3, and cten), and their encoding proteins are localized to the cytoplasmic side of focal adhesions. (rupress.org)
By miniaturizing the tool, we can probe the role of cell mechanics at the length scale appropriate to the size of cells and their proteins. (washington.edu)
Our bodies can be viewed as a hierarchical system where our organs are composed of different tissues, which are defined by cells and the properties of the proteins they express. (washington.edu)
These properties will act mediating the action of cell adhesion proteins, regulating cell behavior and causing tissue regeneration. (bvsalud.org)

In isolated murine SMCs, sLR11 caused membrane ruffle formation via activation of focal adhesion kinase/ERK/Rac1 accompanied by complex formation between uPAR and integrin αvβ3, a process accelerated by Ang II. (jci.org)
Protein kinase C regulates human pluripotent stem cell self-renewal. (rndsystems.com)
Focal adhesion kinase (FAK) is a protein tyrosine kinase (PTK) crucial in regulation of cell migration and proliferation. (molcells.org)
In addition to its canonical roles as a cytoplasmic kinase downstream of integrin and growth factor receptor signaling, recent studies revealed new aspects of FAK action in the nucleus. (molcells.org)
Interestingly, nuclear translocation of FAK becomes prominent in kinase-inhibited conditions such as in de-adhesion and pharmacological FAK inhibition, while a small fraction of nuclear FAK is observed a normal growth condition. (molcells.org)
We found that 4HPR-induced inhibition of PC cell migration and invasion correlates with decreased FAK and AKT phosphorylation, activation of the glycogen synthase kinase 3β(GSK3β) and β-catenin destabilization. (biomedcentral.com)
Fer kinase also regulates cell cycle progression in malignant cells. (umbc.edu)
In experiments probing for a role of PKCa in human laminin-2-integrin-mediated cell adhesion and spreading of PC12 cells, we observed unexpected enhancements of adhesion, spreading and stress fiber formation to 1 mM Go¨6976 with concomitant increase in membrane translocation of PKCd and autophosphorylation of focal adhesion kinase (FAK). (mirnamimic.com)
Pharmacologic blockade of PKC inhibits cell adhesion and spreading as well as focal adhesion formation and focal adhesion kinase (FAK) phosphorylation [13,14]. (mirnamimic.com)
Moreover, Wnt-5a is known to inhibit migration of breast epithelial cells in culture and expression of Wnt-5a potentiates activation of the receptor tyrosine kinase DDR1, a collagen receptor implicated in cell adhesion and migration. (lu.se)
We demonstrate that DARPP-32 inhibits MCF-7 cell migration and that this effect requires phosphorylation of Threonine-34, an event catalyzed by protein kinase A (PKA) and strongly induced by detachment of cells from the culture substrate. (lu.se)
Finally, we found that phospho-DARPP-32 inhibited the activity of the focal adhesion kinase (FAK) in MCF-7 breast cancer cells, and that MCF-7 cells expressing phospho-DARPP-32 displayed less filopodia formation. (lu.se)

Comparing HESC controls with HESCs deficient in SRC-3, gene enrichment analysis of the differentially expressed gene set revealed an overrepresentation of genes involved in chromatin remodeling, cell proliferation/motility, and programmed cell death. (frontiersin.org)
Integrins are key regulators of gene expression, cell proliferation and migration. (europa.eu)
Cell adhesion plays a critical role in cell communication, cell migration, cell proliferation, and integration of medical implants with tissues. (mysciencework.com)
Nuclear FAK promotes p53 and GATA4 degradation via ubiquitination, resulting in enhanced cell proliferation and reduced inflammatory responses. (molcells.org)
The proliferation of RCC cells was analyzed using MTT and EdU incorporation assays. (thno.org)
In addition, we found that BMP-2, a 4HPR target with antiangiogenic activity, decreased prostate cancer cell proliferation, migration and invasion by down-regulating the pathway described involving AKT phosphorylation, β-catenin stability and cyclin D1 expression. (biomedcentral.com)
As a consequence, 4HPR led to the regulation of genes controlling cell proliferation, angiogenesis and metastasis. (biomedcentral.com)
They are endowed with GTP hydrolytic activity, mainly involved in cytoskeleton rearrangements and cell motility, but also involved in cell proliferation, transformation and differentiation [ 2 ]. (biomedcentral.com)
This occurs naturally through a process known as mechanotransduction, which is the conversion of mechanical factors into biochemical changes, and can influence cellular functions such as proliferation, differentiation, migration, and apoptosis. (washington.edu)
Cell proliferation was tested by MTT assay. (spandidos-publications.com)
It is reportedly involved in many physiological functions of cells, including apoptosis, proliferation, invasion and angiogenesis ( 5 ). (spandidos-publications.com)
In order to eliminate the interference from the transplanted cells themselves, fetal stem cells were used for transplantation because the proliferation and differentiation potentials of fetal stem cells are superior to the adult ones ( 8 ). (ijbs.com)

Although, the non-muscle myosin II holoenzyme (myosin) is a molecular motor that powers contraction of actin cytoskeleton networks, recent studies have questioned the importance of myosin motor activity cell and tissue shape changes. (elifesciences.org)
Feedback interactions from mechanical and biochemical signals within focal adhesion and the F-actin cytoskeleton coordinate the behavior of the protrusive and contractile lamella by promoting and sustaining the proper spatial and temporal control in the cell [3]. (technologybooksindustrialprojectreports.com)
A common theme of these processes is a dynamic reorganization of actin cytoskeleton which has now emerged as a major switch control mainly carried out by Rho and Rac GTPase subfamilies, playing an acknowledged role in adaptation of cell motility to the microenvironment. (biomedcentral.com)
Focal adhesions are integrin-mediated cell matrix junctions connecting the ECM to the actin cytoskeleton. (rupress.org)
A key aspect to our physiology is how all the small parts come together, e.g. cytoskeleton, focal adhesions, cell-cell contacts, cell membrane, etc. (washington.edu)

Required for talin assembly into nascent adhesions forming at the leading edge toward the direction of the growth factor. (gliome.org)

Integrin signals mediate anchorage-dependence of cell growth, while growth of cancer cells is anchorage-independent. (europa.eu)
Integrin-mediated retention of phosphorylated caveolin in focal adhesions inhibits raft internalisation, which occurs upon its recruitment to caveolae after cell detachment. (europa.eu)
Negatively regulates integrin alpha-L/beta-2 (LFA-1) polarization and adhesion induced by T-cell receptor. (gliome.org)
Integrin-based adhesions play critical roles in cell migration. (hal.science)
β1 integrin is essential for pancreatic beta-cell development and maintenance in rodents and humans. (oncotarget.com)
However, the effects of a temporal beta-cell specific β1 integrin knockout on adult islet function are unknown. (oncotarget.com)
We utilized a mouse insulin 1 promoter driven tamoxifen-inducible Cre-recombinase β1 integrin knockout mouse model (MIPβ1KO) to investigate β1 integrin function in adult pancreatic beta-cells. (oncotarget.com)
This data demonstrates that β1 integrin is required for the maintenance of glucose homeostasis through postnatal beta-cell function and expansion. (oncotarget.com)
The role of β1 integrin in maintaining optimal islet function has previously been identified in vitro using cell lines and isolated islets. (oncotarget.com)
The formation of focal adhesions on 2D surfaces begins with integrin clustering upon conversation with the ECM. (technologybooksindustrialprojectreports.com)
Small transient integrin-associated nascent adhesions form first followed by the formation of larger more stable fibrillar adhesion with actin stress fibers which facilitate cell distributing and migration [7]. (technologybooksindustrialprojectreports.com)
Biological activities of laminin are mediated through binding to a subset of integrin family of cell adhesion receptors, a3b1, a6b1, a6b4, and a7b1 [2]. (mirnamimic.com)
PKC family of serine-threonine kinases plays key roles in many of the signaling pathways that control cellular function, including those mediated through integrin signaling. (mirnamimic.com)
In PC12 cells, we recently showed that the engagement of integrin a3b1 to a binding sequence within the human laminin a2 chain was sufficient to recruit PKCa and PKCd to the integrin receptor [6]. (mirnamimic.com)

It plays important roles in neuronal polarization and neurite development, cytoskeletal reorganization, cell migration, growth factor signaling, and the regulation of cell-cell interactions mediated by adherens junctions and focal adhesions. (umbc.edu)
Collective motility leads to movement of cohorts of cells which maintain the adherens junctions and move by photolytic degradation of matrix barriers. (biomedcentral.com)

These adhesions are usually physically linked to large, readily visualized actomyosin bundles [13] , and their appearance correlates inversely with motility, such that they are conspicuously absent in cells migrating within a three-dimensional (3D) substrate. (cellmigration.org)
Knocking down PARVB expression in vitrocaused apparent decreases in cell migration and wound healing, implying that PARVB has a crucial role in cellular motility. (refine.bio)
We have defined the role of Gp78 (also known as autocrine motility factor receptor (AMFR)), a cancer-associated receptor and E3 ubiquitin ligase in endoplasmic reticulum (ER) associated degradation, in ER-mitochondria interaction and mitophagy. (ubc.ca)
4HPR was able to decrease AKT phosphorylation also when powerfully upregulated by IGF-1 and, consequently, to impair IGF-1-stimulated cell motility. (biomedcentral.com)
One particular of them is paxillin, a crucial focal adhesion protein that is definitely important for cell matrix adhesion, cell motility and migration. (hormonespathway.com)
Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. (molvis.org)
These two modes of cell movement are interconvertible and several moving cells, including tumor cells, show an high degree of plasticity in motility styles shifting ad hoc between mesenchymal or amoeboid movements. (biomedcentral.com)
This review will focus on the role of Rac and Rho small GTPases in cell motility and in the complex relationship driving the reciprocal control between Rac and Rho granting for the opportunistic motile behaviour of aggressive cancer cells. (biomedcentral.com)
Among other members, we will focus our attention on the Rac and Rho subfamilies, as they are the main effectors of cell motility. (biomedcentral.com)

Cell type and tissue specific alterations in fine GAG structure, which are strictly predetermined [ 8 - 10 ], allow these molecules to modulate with high specificity different cellular processes [ 7 ]. (hindawi.com)
Recently, AGR2 was identified as a crucial component of the cellular machinery responsible for maintaining the epithelial phenotype, thereby interfering with the induction of mesenchymal phenotype cells by TGF-ß effects in cancer. (muni.cz)
One important mechanical cue, known to influence cell migration, is the gradient of substrate elastic modulus (E). However, the cellular microenvironment is viscoelastic and hence the elastic property alone is not sufficient to define its material characteristics. (ncbs.res.in)
Hence, the role of viscoelasticity needs to be investigated at depth in various cellular contexts. (ncbs.res.in)
While it is known that cells migrate when presented with a substrate with a rigidity gradient, cellular behavior in response to viscoelastic gradient has never been investigated. (ncbs.res.in)
The findings of this paper not only reveal a completely novel cellular taxis or directed migration, it also improves our understanding of cell mechanics significantly. (ncbs.res.in)
We previously demonstrated the critical involvement of SRC-2 in murine embryo implantation as well as in human endometrial stromal cell (HESC) decidualization, a cellular transformation process required for trophoblast invasion and ultimately placentation. (frontiersin.org)
These molecular findings are supported at the cellular level by the inability of HESCs to morphologically transform from a stromal fibroblastoid cell to an epithelioid decidual cell when endogenous SRC-3 levels are markedly reduced. (frontiersin.org)
Participates in a variety of cellular processes such as vesicle mediated transport, cell adhesion, cell polarization and cell migration. (gliome.org)
The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Integrins are important regulators of cellular differentiation, migration, maturation, and survival [ 1 - 3 ]. (oncotarget.com)
We employ a range of techniques, such as covalent magnetic tweezers to study mechanochemical signaling, microfabricated substrates to apply controlled mechanical forces, and live-cell imaging to observe cellular responses in real time. (shubhasis-haldar-cmt.com)
These findings established a new paradigm of FAK signaling from cellular adhesions to the nucleus. (molcells.org)
Work in the Nabi lab focuses on the cell biology of cancer and the use of super-resolution microscopy to study cellular domains. (ubc.ca)
The discrepancy of cellular migratory behavior when focal adhesion-related components in 2D and 3D are altered could indicate that focal adhesions in 3D if they exist may carry out different functions [12]-[14]. (technologybooksindustrialprojectreports.com)
In this dissertation, I investigate the interplay of confinement and cellular metabolism in breast cancer cell migration using an engineered collagen microtrack platform. (vanderbilt.edu)
Finally, we establish cellular metabolic phenotype as a stable, heritable trait of cancer cells that can determine cell response to mechanical cues and migration ability. (vanderbilt.edu)
Importantly, enhanced cellular behavior and membrane translocation of PKCd induced by Go¨6976 was retained in siRNA-transfected PC12 cells to knockdown PKCa expression. (mirnamimic.com)
Synthetic peptides or recombinant fragments containing bio- logically active motifs have been shown to partially mimic the effects of native laminin in various cellular functions, such as cell adhesion, spreading, migration, neurite outgrowth, and tumor metastasis [6-10], suggesting that laminin-derived-short peptide- modified biomaterials may have therapeutic potential in tissue regeneration applications. (mirnamimic.com)
If we can understand how cells are guided by mechanics, then we can alter or direct cellular properties and responses in order to influence large changes in tissue and organs. (washington.edu)

In addition, LIMCH1-depleted HeLa cells exhibited a decrease in the number of actin stress fibers and focal adhesions, leading to enhanced cell migration. (nih.gov)
The best-characterized molecules are Rho, which controls the stress fibers and focal adhesion formation, and Rac and Cdc42, which regulate membrane ruffling, and filopodium formation, respectively. (biomedcentral.com)

PGs, molecules which consist of a protein core that is covalently modified with GAG chains, are distributed both to the ECM "proper" associated with the cell membrane as well as located to intracellular compartment. (hindawi.com)
It has roles in cell division, membrane trafficking, and cell migration. (biologists.com)
Modulates the targeting of talins (TLN1 and TLN2) to the plasma membrane and their efficient assembly into focal adhesions. (gliome.org)
PBP 10, an antibacterial, cell membrane-permeant rhodamine B-conjugated peptide derived from the polyphosphoinositide binding site of gelsolin, interacts selectively with both lipopolysaccharides (LPS) and lipoteichoic acid (LTA), the distinct components of gram-negative and gram-positive bacteria, respectively. (upenn.edu)
We study the role of plasma membrane domain effectors galectin-3 and caveolin-1 in focal adhesion dynamics and tension in metastatic cancer cells. (ubc.ca)
We have developed network analysis of dSTORM super resolution microscopy to define the molecular architecture of caveolae and scaffolds and are now studying the structural changes in these membrane domains associated with tumor cell migration and mechanical stress. (ubc.ca)
Plasma membrane domain organization regulates EGFR signaling in tumor cells. (ubc.ca)
Breast cancer metastasis is initiated when cells break through basement membrane and migrate through the surrounding tissue before spreading throughout the body to colonize secondary sites. (vanderbilt.edu)

These data point to 4HPR as a negative regulator of AKT phosphorylation, effectively targeting the β-catenin pathway and inducing a relatively benign phenotype in prostate cancer cells, limiting neoangiogenesis and cell invasion. (biomedcentral.com)
HGF c Met signaling can induce paxillin phosphorylation at its tyrosine residue, which in turn promotes tumor progression by enhancing tumor cell migration and spread.ten Activating c Met mutations are already proven to increase paxillin phosphorylation in SCLC.5 Furthermore, paxillin has become proven to be very expressed, and its gene from time to time amplified or mutated in NSCLC 11. (hormonespathway.com)
While Akt Ser 473 phosphorylation was readily detectable in AND-34 +/+ lens epithelial cells, it was markedly reduced in the AND-34 −/− lens epithelium. (molvis.org)
We find that modulating glucose metabolism by inhibiting glycolysis or oxidative phosphorylation can shift EMT status to alter migration ability. (vanderbilt.edu)
In addition, we found that Wnt-5a could directly trigger a cAMP response that resulted in phosphorylation of DARPP-32 and stimulation with Wnt-5a was necessary for DARPP-32 mediated inhibition of cell migration in wound healing assay. (lu.se)

In addition, immunofluorescence showed the formation of stress fibers and vinculin foci in cells without AGR2 and in response to TGF-ß treatment, with synergistic effects observed. (muni.cz)
In addition the N&B analysis shows paxillin aggregated predominantly at these focal contacts which are next to collagen fibers. (technologybooksindustrialprojectreports.com)

Quantifying the mechanics and growth of cells and tissues in 3D using high resolution computational models. (crossref.org)
Non-muscle cell contractility is critical for tissues to adopt shape changes. (elifesciences.org)
Cell-cell adhesion maintains epithelial tissues, supports functional contacts between specialized cells, and can facilitate directed migration (for example, radial glia can guide neuronal progenitor cells to specific layers of the brain). (cellmigration.org)
Processes involved in later stage disease, like development of androgen independence as a consequence of androgen depletion therapy, neoangiogenesis and homing of metastatic cells in lymphatic or bone tissues are generally undetectable at early stages. (biomedcentral.com)
However, the potential toxic effects of HLCL-61 hydrochloride on normal cells and tissues are not well understood. (thebiotek.com)
The current limitations in the use and study of HLCL-61 hydrochloride include its potential toxic effects on normal cells and tissues and the lack of clinical data. (thebiotek.com)
Moreover, these properties and responses can serve as mechanical signatures that allow us to diagnose diseased states in our cells and tissues. (washington.edu)
Tissue engineering is a strategy very used to obtain functional repairing through the development of biological substitutes that can restore, keep or substitute damaged tissues or organs 31 , through the combination of scaffolds biocompatible with live cells and/or bioactive molecules 19 . (bvsalud.org)
The use of nanotechnology plays an important role in tissue engineering because the properties that this technology adds to the material, such as the greater surface area and greater roughness, improve the physical-chemical properties which mimic those of the natural tissues and organs 31 . (bvsalud.org)

NSC 34 cells were well differentiated in very low serum medium with prolonged neuritic processes, a morphological marker of neuronal cell maturation and differentiation. (hormonespathway.com)
Moreover, proliferating cell nuclear antigen (PCNA) and CD31 showed co-localization with α-SMA, suggesting the differentiation of hBMSCs into epithelial cells and myofibroblasts/fibroblasts. (ijbs.com)
In order to be effective and integrated to the receiving area, the bone graft is required to allow a strong cell adhesion, interacting with several molecules to induce migration, differentiation, and thus the mineralization of the new bone on the graft. (bvsalud.org)
Notwithstanding, to achieve the effectiveness and integration of the grafted tissue to the receptor site, it is necessary a strong cell adhesion, so that it demands many molecules interaction to induce cell differentiation and the bone matrix mineralization formed onto the graft. (bvsalud.org)

Together with dynactin, dynein regulates centrosomal orientation to establish and maintain cell polarity, controls focal adhesion turnover and anchors microtubules at the leading edge. (biologists.com)
These signals are also required for the dissolution of some adhesions (turnover) as the cell advances. (cellmigration.org)
Like nascent adhesions, they also tend to either disassemble (turnover) or grow and elongate into focal adhesions. (cellmigration.org)
Microtubules regulate the turnover of adhesion sites, and, in turn, focal adhesions promote cortical microtubule capture and stabilization in their vicinity, but the underlying mechanism is unknown. (kent.ac.uk)
5 μm (fibrillar adhesion) with slower turnover rates [3] [8]. (technologybooksindustrialprojectreports.com)

Since angiogenesis and metastatic spread are strictly related, in this study we analyzed the regulation of multiple signaling pathways responsible for cancer cell invasion. (biomedcentral.com)
The primary tumor microenvironment, composed primarily of collagen, imparts mechanical challenges upon cancer cells that regulate their migration ability, metabolism, and metastatic potential. (vanderbilt.edu)
In addition we analyse the role of these GTPases in cancer progression and metastatic dissemination. (biomedcentral.com)

Changes in transcripts associated with the focal adhesion pathway were validated by RT-qPCR of COL4A1, COL4A2, FLNA, VAV3, VEGFA, and VINC mRNAs. (muni.cz)

Plays a role in endocytosis mediated by clathrin and AP-2 (adaptor protein complex 2). (gliome.org)
and liprins are recruited to focal adhesions by the adaptor protein KANK1, which directly interacts with the major adhesion component, talin. (kent.ac.uk)
AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. (molvis.org)

Tensin is a focal adhesion molecule that binds to actin filaments and participates in signaling pathways. (wikipedia.org)
Consequently, we aimed to assess miR-637 expression in gliomas to determine the effects of miR-637 on glioma cell migration and invasion, as well as to explore the possible signaling pathways involved. (scirp.org)
This objective integrates into a bigger project that also aims to identify molecular mechanisms by which caveolin regulates anchorage-dependent signalling pathways and cell cycle progression and to identify oncogenes that induce constitutive surface localization of rafts (and hence Rac), and/or changes in phosphocaveolin localization. (europa.eu)
These studies have served to highlight the role of AND-34/BCAR3 and p130Cas in cell adhesion and migration signaling pathways. (molvis.org)
In the current study, the anti‑invasive activities and associated signaling pathways of celastrol were determined in ovarian cancer cells. (spandidos-publications.com)
Targeting activated Gα subunits to specific cells in vivo permits identification of downstream effector pathways independent of receptor activation, and thus permits insight into disease mechanisms otherwise impossible to study in vivo . (medscape.com)

It has been shown to inhibit the growth of cancer cells and induce apoptosis in vitro and in vivo. (thebiotek.com)

To bridge this gap, in this study, we investigated the influence of the gradient of viscous property of the substrate, as defined by loss modulus (G) on cell migration. (ncbs.res.in)
STATEMENT OF SIGNIFICANCE: While the effect of substrate elastic modulus has been investigated extensively in the context of cell biology, the role of substrate viscoelasticity is poorly understood. (ncbs.res.in)
In addition when cells are in the 3D environment there is a continuum of migration modes that are determined by both matrix substrate and intrinsic contractility of the cell [7] and focal adhesions may not be needed for migration. (technologybooksindustrialprojectreports.com)

Scheme depicts the process of adhesion maturation. (cellmigration.org)
Focal complexes are adhesions in the early stages of maturation. (cellmigration.org)
Fibrillar adhesions represent an extreme in the maturation of adhesions and are not generally seen in migrating cells. (cellmigration.org)

Is the mechanical activity of epithelial cells controlled by deformations or forces? (crossref.org)
pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD. (molcells.org)
Western blot analysis and in situ hybridization confirmed the presence of AND-34 RNA and protein in lens epithelial cells, particularly at the lens equator. (molvis.org)
These results demonstrate the loss of AND-34 dysregulates focal adhesion complex signaling in lens epithelial cells and suggest that AND-34-mediated signaling is required for maintenance of the structural integrity of the adult ocular lens. (molvis.org)
These results suggest that DARPP-32 restricts the migration of breast epithelial cells via both a transcription dependent mechanism that involves CREB and a transcription independent mechanism affecting FAK. (lu.se)

Required for focal adhesion dynamics. (gliome.org)
Since each single protein molecule has its own contribution to the overall FA dynamics and thereby the cell migration, the force response of a single protein is of prime importance to understanding the underlying mechanism. (shubhasis-haldar-cmt.com)
Our characterization of the proteome and transcriptome of tumor cell pseudopodia has identified multiple pseudopod-localized effectors whose role in pseudopod-specific actin dynamics and focal adhesion tension is being studied. (ubc.ca)

We find that this priming in confinement correlates with increased mitochondrial localization at the leading edge of the cell, and that disrupting focal adhesion formation by knocking out vinculin inhibits memory and mitochondrial localization. (vanderbilt.edu)

Perturbation of this interaction, including a single point mutation in talin, which disrupts KANK1 binding but not the talin function in adhesion, abrogates the association of microtubule-stabilizing complexes with focal adhesions. (kent.ac.uk)

Our findings demonstrate that by multiplexing these techniques we have the ability to spatially and temporally quantify focal adhesion assembly and disassembly in 3D space and allow the understanding tumor cell invasion in a more complex relevant environment. (technologybooksindustrialprojectreports.com)

1) Background: Reactive oxygen species (ROS) play a crucial role in the preparation of the normal wound healing response. (mdpi.com)
Directed cell migration plays a crucial role in physiological and pathological conditions. (ncbs.res.in)
In medical research, HLCL-61 hydrochloride can play a crucial role in drug development and clinical trials. (thebiotek.com)

Second high cytoplasmic fluorescence background may give low signal-to-background ratio that hinders the detection of focal adhesions [10]-[12]. (technologybooksindustrialprojectreports.com)

Several molecules are involved in the process of cell adhesion to bone grafts, highlighting the role of integrins, the focal adhesion mechanism, the influence of the collagen matrix, and the activity of alkaline phosphatase in bone matrix formation. (bvsalud.org)

In addition to basic science investigations in such areas as tumor cell invasion, oncogenes, immunotherapy and genetic inheritance, our scientists and physicians participate in national cancer trials. (upstate.edu)

A small percentage of AND-34 −/− mice show distinctive small white eye lesions resulting from the migration of ruptured cortical lens tissue into the anterior chamber. (molvis.org)

As a well-defined clutch-accelerator system, the proper functioning of FA depends on its constituent mechanical linkages, which both transmit and transduce the mechanical force into the cells. (shubhasis-haldar-cmt.com)
From the beating heart to the growth of our brain, mechanical forces play a pivotal role in sculpting life itself. (shubhasis-haldar-cmt.com)
Introduction The study of focal adhesions in the two-dimensional (2D) environment has led to an in depth understanding of their protein composition [1] structure [2] and their role in cell migration as well as mechanical sensing. (technologybooksindustrialprojectreports.com)
Through studying the coordinated activity of cells and quantifying their mechanical properties, we can understand normal and diseased physiology at a fundamental level. (washington.edu)

and microtubule targeting, which may contribute to adhesion disassembly. (cellmigration.org)

PARVB is known to play a critical role in actin reorganization and focal adhesions. (refine.bio)

regions of paxillin in TG-101348 cell produced in 3D collagen matrices. (technologybooksindustrialprojectreports.com)
The role of paxillin in LCNEC and carcinoid has not been effectively studied. (hormonespathway.com)

This non-migratory phenotype was similarly produced in both cell lines by stable silencing of β-catenin. (biomedcentral.com)
The anti-migratory effects of Wnt-5a and DARPP-32 were reduced by dominant negative-CREB, which suggests that CREB plays a functional role in this signalling mechanism. (lu.se)

The development of new primary human cell culture technologies such as 3D culture, microfluidics and microfabrication in combination with human induced pluripotent stem cell (iPSC) derived models promise to generate more relevant human physiological systems for drug testing 11 . (nature.com)
Clinical and cell-based studies as well as investigations on mice have demonstrated pivotal roles for each SRC in numerous physiological and pathophysiological contexts, underscoring their functional pleiotropy. (frontiersin.org)

We cultured human mesenchymal stem cells (hMSCs) on a collagen-coated polyacrylamide gel with constant storage modulus (G) but with a gradient in the loss modulus (G). We found hMSCs to migrate from high to low loss modulus. (ncbs.res.in)
breast malignancy cell collection MDA-MB-231 either cultured in Matrigel [15] or type I collagen matrix [16]. (technologybooksindustrialprojectreports.com)
Using our collagen microtrack platform, we find that breast cancer cells in confinement exhibit increased velocity and cell-generated matrix strains. (vanderbilt.edu)
We next investigate the role of glucose metabolism and epithelial-to-mesenchymal transition (EMT) in migration through collagen microtracks. (vanderbilt.edu)
Following in vitro exposure to CTX, hBMSCs showed decreased cell growth in a dose- and time-dependent manner, accompanied by increased expressions of collagen-I/III, and CD31. (ijbs.com)

This is inefficient to TG-101348 image structures that are sparse in 3D such as a cell protrusion. (technologybooksindustrialprojectreports.com)

One of the key processes in tumor progression and metastasis is epithelial to mesenchymal transition (EMT), in which TGF-ß signaling plays important roles. (muni.cz)
The above findings indicate that DOK5 plays a key role in the invasion, progression, and the metastasis of cancer. (hindawi.com)
PARVB overexpression increases migration capability and defines high risks for endophytic growth and metastasis in tongue squamous cell carcinoma. (refine.bio)
Tongue squamous cell carcinoma (TSCC) varies in characteristics even in early stages and is mainly classified into three subtypes, which are superficial, exophytic and endophytic types, based on a macroscopic appearance of tumor growth.Of these subtypes, endophytic tumor has a poorer prognosis because of its invasive feature and higher frequency to have metastasis. (refine.bio)
Metastasis accounts for 90% of cancer-associated mortality in patients with renal cell carcinoma (RCC). (thno.org)
PC cells usually remain confined in the organ, while a small proportion of carcinomas acquire the ability to metastasize and approximately 80% of patients who have died of advanced hormone refractory PC have clinical evidence of bone metastasis. (biomedcentral.com)
Taken together, this work identifies a key link between metabolism and confined migration in breast cancer cells and highlights potential avenues for therapeutics to treat breast cancer metastasis. (vanderbilt.edu)

PKC activation by phorbol myristate acetate has been shown to induce the adhesion, spreading, and migration of cells [12]. (mirnamimic.com)

We identified significant changes in transcripts associated with focal adhesion and eicosanoid production, in particular arachidonic acid metabolism. (muni.cz)
Since PGE2 is a product of arachidonic acid metabolism, its lowered concentration in media from AGR2-knockout cells was confirmed by ELISA. (muni.cz)
Scientists working in basic, translational, and clinical cancer metabolism research are invited to join the Academy in New York on April 17th to discuss the intersection between cell signaling and metabolism. (nyas.org)
Further, inducing EMT can modulate cell metabolism to affect migration ability, indicating a link between cancer cell bioenergetics and EMT. (vanderbilt.edu)

The consecutive stages of cancer growth and dissemination are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
Importantly, cell-associated and tumor microenvironment glycosaminoglycans (GAGs)/proteoglycan (PG) content and distribution are markedly altered during tumor pathogenesis and progression. (hindawi.com)
It is now increasingly recognized that the microenvironment plays a critical role in the progression of tumors. (hindawi.com)
The consecutive steps of tumor growth, local invasion, intravasation, extravasation, and invasion of anatomically distant sites are obligatorily perpetrated through specific interactions of the tumor cells with their microenvironment. (hindawi.com)
Cell-associated and tumor microenvironment GAG content and distribution is markedly altered during tumor pathogenesis and progression [ 11 , 12 ]. (hindawi.com)
It has been postulated that focal adhesions TG-101348 may not form at all due to the pliability of the microenvironment [11]. (technologybooksindustrialprojectreports.com)
Cancer cells migrating through the primary tumor microenvironment must navigate confined spaces in the tissue. (vanderbilt.edu)

The underlying idea is usually that if a part of a cell can sense the glass surface the behavior of the TG-101348 entire cell may be biased by the properties of the surface stiffness. (technologybooksindustrialprojectreports.com)

Inhibition of TGF-β1 Signaling by IL-15: A Novel Role for IL-15 in the Control of Renal Epithelial-Mesenchymal Transition: IL-15 Counteracts TGF-β1-Induced EMT in Renal Fibrosis. (rndsystems.com)
Notably, the HSPA12A overexpression-induced inhibition of lactate export and migration were abolished by CD147 overexpression. (thno.org)
In preliminary experiments examining the role of PKCa in cell adhesion and spreading, we found that Go¨6976, a widely used, nonglycosidic indolocarbazole inhibitor of PKCa/b, had effects that could not be explained by inhibition of PKCa/b alone. (mirnamimic.com)
DARPP-32 mediated inhibition of cell migration proved to be dependent on DDR1 expression, consolidating a functional relevance of the interaction between DARPP-32 and DDR1. (lu.se)

These adhesions generate signals that activate Rac, promoting actin polymerization and preventing myosin II engagement in the lamellipodium. (cellmigration.org)

We also found that SRC-3 depletion in cultured HESCs results in a significant attenuation in the induction of a wide-range of established biomarkers of decidualization, despite exposure of these cells to a deciduogenic stimulus and normal progesterone receptor expression. (frontiersin.org)
Considerable amounts of the shed soluble form of the LDL receptor relative LR11 (sLR11) produced by intimal SMCs enhance SMC migration in vitro via upregulation of urokinase-type plasminogen activator receptor (uPAR) expression. (jci.org)
A specific inhibitor of PKCd, rottlerin, completely abrogated Go¨6976-dependent increase in PC12 cell adhesion to laminin as well as the activation of small GTPases, Rac1 and Cdc42, that are downstream of PKCd in adhesion receptor signaling. (mirnamimic.com)

In addition it depends on Rac1-mediated cell polarization and lamellipodia formation. (biomedcentral.com)
siRNA knockdown of Rac1 and Cdc42 expression inhibited cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)

14 - 18 Two principal cannabinoid receptors have been described (CB1, which is predominant in neurons, and CB2, which is localized in immune cells and peripheral tissue cells). (arvojournals.org)

This specific project aims to identify sequences within caveolin involved in regulation of raft internalisation, cell cycle progression and anchorage-dependent cell growth. (europa.eu)

Rho activation is thought to generate focal adhesions and actin bundling due to increased myosin II activity. (cellmigration.org)

These findings imply that both AGR2 downregulation and TGF-ß have a role in focal adhesion formation and cancer cell migration and invasion. (muni.cz)
Next, the effects of miR-637 on glioma cell migration and invasion were determined by using the transwell assay. (scirp.org)
In addition, miR-637 suppressed glioma cell migration and invasion. (scirp.org)
Our findings suggest that miR-637 inhibits glioma invasion and migration and could be a potential diagnostic marker for gliomas. (scirp.org)
We found that 4HPR impairs DU145 and PC3 prostate cancer cells migration and invasion by down-regulating FAK and AKT activation and by enhancing β-catenin degradation, causing the downregulation of target genes like cyclin D1, survivin and VEGF. (biomedcentral.com)
Cell migration was detected by wound healing and Transwell assays, while cell invasion was detected by a Matrigel‑coated Transwell method. (spandidos-publications.com)

Mutations on DLC-1 perturb the interaction with cten and disrupt the focal adhesion localization of DLC-1. (rupress.org)
These results provide a novel mechanism whereby the SH2 domain of cten-mediated focal adhesion localization of DLC-1 plays an essential role in its tumor suppression activity. (rupress.org)

Overall, our data highlights that myosin activity is required for rapid cell contraction and tissue folding in developing Drosophila embryos. (elifesciences.org)
Cell shape changes and cell movements drive these tissue-scale transformations. (elifesciences.org)
In this study, we have for the first time incorporated cells from the most devastating brain cancer (glioblastoma) from primary brain tumor tissue from our patients into the BS. (nature.com)
The research conducted by central investigators is quite broad, ranging from cell and molecular biology to tissue engineering, biophysics and nanobiology/medicine. (upenn.edu)
Description: A sandwich quantitative ELISA assay kit for detection of Human Granzyme A (GZMA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (1elisakits.com)
Description: Quantitativesandwich ELISA kit for measuring Human granzyme A (GZMA) in samples from serum, plasma, cell culture supernates, tissue homogenates. (1elisakits.com)
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Granzyme A (GZMA) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. (1elisakits.com)
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Granzyme A (GZMA) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species. (1elisakits.com)
With regard to cancer treatment, celastrol has been shown to exert considerable cell-killing effects and to shrink xenografted tumors in tissue and animal models of various malignancies including cancers of the prostate ( 16 - 18 ), breast ( 19 ), liver ( 20 , 21 ) and lung ( 22 , 23 ). (spandidos-publications.com)
This paper is a systematic review of the literature on the mechanisms of cell adhesion to bone grafts associated to nanotechnology, describing the importance and the role of those molecules in the adhesion and thus in tissue regeneration. (bvsalud.org)
Accurate identification of these mechanisms of cell adhesion is essential for further advancement in tissue engineering, such as the production of biological bone substitutes that achieve a better clinical outcome. (bvsalud.org)

1) The role of non-caveolar scaffold domains and the galectin lattice in cancer cell migration and focal adhesion tension. (ubc.ca)

The phospho-caveolin-1 scaffolding domain dampens force fluctuations in focal adhesions and promotes cancer cell migration. (ubc.ca)
Hu G, Liu P, Feng J, Jin Y. Transplantation with Bone Marrow Stromal Cells Promotes Wound Healing Under Chemotherapy through Altering Phenotypes. (ijbs.com)

Medial-to-intimal migration of SMCs is critical to atherosclerotic plaque formation and remodeling of injured arteries. (jci.org)
Required for uropodium formation and retraction of the cell rear during directed migration. (gliome.org)
They are larger than nascent adhesions, depend on myosin II for their formation and maintenance, and reside at the boundary of the lamellum and lamellipodium [10] , [11] . (cellmigration.org)
Whereas the formation of focal adhesions requires actomyosin-mediated contractile force mediated by RhoA , podosome formation is stimulated by a local loss of contractility and the recruitment of negative regulators of RhoA, such as p190RhoGAP [16] . (cellmigration.org)
FAK signaling mediates cell spreading and lamellipodia formation in PC12 cells. (mirnamimic.com)

These results may provide a molecular explanation for the role of caveolin-1 as tumour suppressor and provide insight into the mechanism of anchorage-independent growth, one of the hallmarks of cancer. (europa.eu)
Our results clarified the significant role of DOK5 in the prognosis of GC and offer a potential mechanism by which DOK5 expression might monitor tumor immunity-regulation of the infiltration of immune cells in GC. (hindawi.com)
In an effort to elucidate the mechanism of bacterial killing by PBP 10, we compared its effects on artificial lipid bilayers and eukaryotic cell membranes with the actions of the mellitin, magainin II, and LL37 peptides. (upenn.edu)
15 , 20 - 22 In vivo, AEA has a short duration of action: it is transported to cells by a carrier-mediated uptake mechanism, and it is hydrolyzed by the enzyme fatty acid amide hydrolase (FAAH). (arvojournals.org)
Go¨6976 also induced laminin-dependent cell adhesion in NIH/3T3 and CV-1 fibroblasts, suggesting of a mechanism that may be common to multiple cell-types. (mirnamimic.com)

T lymphocytes for adoptive cell therapy against hematological cancer. (upstate.edu)
The differential responses of gBS tumors and normal neuronal cells to sustained treatments with anti-cancer drugs temozolomide (TMZ) and doxorubicin (DOX) were investigated. (nature.com)
Here, we performed transcriptomic profiling of A549 lung cancer cells with CRISPR-Cas9 mediated AGR2 knockout with and without TGF-ß treatment. (muni.cz)
However, the specific role of some miRNAs in cancer remains unclear. (scirp.org)
Nevertheless, the role of DOK5 expression in the prognosis of gastric cancer (GC) remains unclear. (hindawi.com)
The Tumor Immune Estimation Resource (TIMER) platform has been used to assess the tumor-infiltrating immune cells of 32 cancer types in a comprehensive way. (hindawi.com)
Lactate export is known to play an important role in cancer cell migration. (thno.org)
2) The role of the cancer-associated ubiquitin ligase Gp78 in the regulation of endoplasmic reticulum-mitochondria contacts and mitophagy. (ubc.ca)
What Holds Cancer Cells Back? (fabiodisconzi.com)
Multi-omics biomarkers of response to the discovered drugs were identified using human breast cancer cell lines. (cdc.gov)
Cancer Lett, 300:215-224.doi:10.1016/j. squamous cell carcinoma in north-eastern Iran. (who.int)
99:209 -215.doi:10.1007/s11060 - 010 - 0129 -5 cell carcinoma-a population-based study in with and without cervical cancer in Tbilisi, Georgia. (who.int)

An interaction of HSPA12A with HRD1 ubiquitin E3 ligase was detected in RCC cells. (thno.org)

Anatomy44

Organisms8

Diseases8

Chemicals and Drugs123

Analytical, Diagnostic and Therapeutic Techniques and Equipment20

Psychiatry and Psychology1

Phenomena and Processes48

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science5

Extracellular18

Proteins18

Kinase12

Proliferation12

Cytoskeleton5

Nascent adhesions forming at1

Integrin14

Adherens junctions2

Motility9

Cellular20

Stress fibers and focal2

Membrane8

Phosphorylation5

Fibers2

Tissues9

Differentiation4

Turnover5

Metastatic3

Pathway1

Adaptor3

Pathways6

Apoptosis1

Substrate3

Maturation3

Epithelial cells5

Dynamics3

Vinculin1

Talin1

Spatially and temporally1

Crucial role3

Cytoplasmic1

Integrins1

Tumor cell1

Cortical1

Mechanical4

Microtubule1

Reorganization1

Paxillin2

Migratory2

Physiological2

Collagen5

Protrusion1

Metastasis7

Induce1

Metabolism4

Microenvironment7

Behavior1

Inhibition4

Signals1

Receptor3

Rac12

Receptors1

Progression1

Myosin-II activity1

Invasion6

Localization2

Tissue11

Tension1

Promotes2

Formation5

Mechanism5

Cancer12

Interaction1