Rodent assays. Medical search. Frequent questions

Those ligands selected from in vitro assays will be subjected to rodent studies, non-human primate studies, and eventually human studies, in collaboration with other scientists within the PET Radiopharmaceutical Sciences section (PRSS, Chief Dr. Victor Pike) and within the Molecular Imaging Branch (MIB, Chief Dr. Robert Innis). (nih.gov)
Based on in vitro assays of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) scavenging analysis, ferrous ion chelating ability, reducing power assessment, and mushroom tyrosinase inhibition evaluation, YEAST-astaxanthin showed anti-oxidative and tyrosinase suppressive properties. (nih.gov)

This Rodent IGFBP-2 ELISA Assay is suited for measuring IGFBP-2 in mouse and rat serum for scientific purposes. (eaglebio.com)
The Mouse/Rat IGFBP-2 ELISA Assay Kit is for Research Use Only. (eaglebio.com)
The Mouse/Rat IGFBP-2 ELISA, is a so-called sandwich-assay. (eaglebio.com)
Eagle Biosciences has quickly become a leading provider of ELISA assay kits, HPLC assay kits, Molecular Biology assays, antibodies, and proteins. (eaglebio.com)
Serologic tests include enzyme-linked immunosorbent assay (ELISA) and Western and strip immunoblot assays. (msdmanuals.com)

Serum samples from 757 persons who had worked with rodents in North America and handled neotomine or sigmodontine rodents were tested for antibodies against Sin Nombre virus, Whitewater Arroyo virus, Guanarito virus, and lymphocytic choriomeningitis virus. (cdc.gov)
Levels of IgG antibodies produced to several food samples were measured by analysis of serum using enzyme-linked immunosorbent assays (ELISAs). (usda.gov)
A third hantavirus was isolated from the cotton rat (Sigmodon hispidus) after antibodies and RT-PCR had identified a target rodent species. (cdc.gov)

Ultimately, 49 chemicals were identified with reproducible AR pathway responses confirmed in at least two in vivo rodent studies. (nih.gov)
The inhibition of lung metastasis of melanoma cells and the increment of the survival rate were demonstrated by feeding YEAST and injecting the intravenous commercial astaxanthin in vivo rodent model. (nih.gov)

The researchers found that other behavioural assays sensitive to stress were affected by male but not female experimenters or T-shirts. (eurekalert.org)
Last but not least, the Core designs and organizes novel behavioural tasks and procedures to study rodent behavior in mice, rats, and hamsters. (tigem.it)

In this study, fleas collected from wild rodents from the Negev desert in southern Israel were tested for RickettsiaDNA by targeting the 16S rRNA (rrs) gene. (datadryad.org)
Hantaviruses occur throughout the world in wild rodents, which shed the virus throughout life in saliva, urine, and feces. (msdmanuals.com)

Rodents are the principal hosts of Sin Nombre virus, 4 other hantaviruses known to cause hantavirus pulmonary syndrome in North America, and the 3 North American arenaviruses. (cdc.gov)
The purpose of this study was to assess the risk for hantavirus and arenavirus infections among persons who work in North America and have close physical contact with neotomine rodents or sigmodontine rodents through their occupations. (cdc.gov)
Because both patients were known to have rodent contact, they were tested for hantavirus infections. (cdc.gov)
Each spring, as rodents emerge from their burrows, Four Corners residents cleaning out storage sheds or woodpiles know to be on the lookout for tiny deer mice, which can carry the deadly Sin Nombre hantavirus. (unm.edu)
In recently published research, Bradfute and his colleagues have developed new gene sequencing methods to confirm the presence of hantavirus, discovered that the virus may also be carried by other rodent species, including house mice, brush mice, white-footed mice, piñon mice and chipmunks, and even identified a potential therapy for an illness that, until now, has had no cure. (unm.edu)
More than 20 types of rodent-borne hantavirus have been identified around the world. (unm.edu)
In a paper published recently in the journal Viruses , Bradfute and colleagues in his lab and the UNM Museum of Southwest Biology reported that they have developed a tool that uses reverse-transcription quantitative real-time polymerase chain reaction technology to rapidly screen the tissue of wild-caught rodents for the presence of hantavirus. (unm.edu)
We have a set of assays that targets any hantavirus," Bradfute says. (unm.edu)
We're seeing [hantavirus] pretty frequently in rodents that are not deer mice. (unm.edu)
And while previous hantavirus cases have been concentrated in the Four Corners region, Bradfute says it appears that rodents elsewhere in New Mexico also carry the Sin Nombre strain. (unm.edu)
Bradfute notes that even if rodent populations carrying the hantavirus are more widespread than previously believed, "The infection rate for Sin Nombre is still very, very low. (unm.edu)
The purpose of this proposed research is to develop a rapid field test system for detection of hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS) infection in humans and rodents using a lateral-flow immunoassay. (sbir.gov)

We developed a species-specific assay for quantitative real-time PCR, then tested the long-term persistence of jumping mouse eDNA on plant material using four herbaceous day nests collected three to six months after occupancy. (nau.edu)
Reveal Q+ for Histamine (PN 9549) is a quantitative lateral flow assay designed to be read on Neogen's Raptor® Integrated Analysis Platform. (neogen.com)
The assay provides quantitative results for histamine in scombroid species of fish and dry animal protein. (neogen.com)

The Core combines basic knowledge of the biological processes underlying animal behavior with systematic use of the whole battery of behavioral tests available for rodents to date. (tigem.it)

We determined the limit of detection for both assays are fewer than eight copies per reaction. (nau.edu)
These FAM-peptide-FMK compounds are both cell permeable and non-cytotoxic during the course of the assay and thus allow the detection of active caspases in living cell systems. (immunochemistry.com)
The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate IETD-pNA. (sigmaaldrich.com)
LANSING, Mich., May 31, 2023 - Neogen® Corporation (NASDAQ: NEOG) announced today that it has launched two new assays for the detection of histamine, Reveal® for Histamine and Reveal Q+ for Histamine. (neogen.com)
Reveal and Reveal Q+ assays are easy-to-use lateral flow tests that utilize a simple water extraction method, providing results in a matter of minutes and eliminating the need to dispose of any chemicals, as is needed with other commercially available histamine detection methods, reducing testing time. (neogen.com)

Reveal for Histamine (PN 9548) is a qualitative lateral flow assay intended for visual screening of histamine at 50 parts-per-million (ppm) in scombroid species of fish, such as tuna and mahi-mahi. (neogen.com)

NICEATM searched the peer-reviewed literature for uterotrophic assay data on 1812 chemicals tested in ToxCast Phase II. (nih.gov)
The uterotrophic assay ( EPA OPPTS 890.1600 ) measures the estrogenic activity of a substance by assessing its effect on the weight of the uterus in a female rodent. (nih.gov)
and may also induce an estrogen-dependent uterotrophic response in rodents [17]. (exposed-skin-care.net)

These results suggest that risk for infection with hantaviruses or arenaviruses usually is low in persons whose occupations entail close physical contact with neotomine or sigmodontine rodents in North America. (cdc.gov)
Pet rat owners and breeders should also be aware of Seoul virus and should practice good hand hygiene and safe rodent handling to prevent infection. (cdc.gov)
Pet rat owners should practice safe rodent handling to prevent Seoul virus infection ( 2 ). (cdc.gov)
The virus exhibits persistent, asymptomatic infection with profuse urinary virus excretion in the ubiquitous rodent vector, Mastomys natalensi s. (folkhalsomyndigheten.se)
tent, asymptomatic infection, with profuse urinary virus excretion in Mastomys natalensi s, the ubiqui- The emergence of this highly virulent and contagious tous and highly commensal rodent host2,3. (folkhalsomyndigheten.se)
Infection is transmitted to humans via inhalation of rodent excreta. (msdmanuals.com)
LCMV causes an asymptomatic infection in its rodent reservoir (eg, mice) and has variable effect on hamster health. (medscape.com)
[ 1 ] Individuals who are exposed to rodents from living conditions or other exposure, such as pet handlers and laboratory personnel, are at particular risk for infection. (medscape.com)

Our goal is to begin to explore these interactions using rodent models of human disease and cDNA microarray assays to reveal patterns of gene expression. (jcvi.org)
On 30 June 22, the Organisation for Economic Cooperation and Development (OECD) published Test #470: Mammalian Erythrocyte Pig-a Gene Mutation Assay , a Test Guideline (TG) that describes an in vivo gene mutation assay that can be combined with other genetic and general toxicology tests to promote the efficient use of animal resources. (hesiglobal.org)
firefly-luciferase assay display that NFLX only can induce estrogenic results by activating the nuclear estrogenic receptors and therefore IFRD2 influencing the ER-regulated gene manifestation. (exposed-skin-care.net)

Specific members of the subfamilies Neotominae and Sigmodontinae in the rodent family Cricetidae ( 11 ) are the principal hosts (reservoirs) of the hantaviruses known to cause HPS in North America and the 3 North American arenaviruses. (cdc.gov)

This TG is the culmination of fruitful discussions over more than a decade among HESI Genetic Toxicology Technical Committee (GTTC) Pig‐a Workgroup members who contributed directly to the Pig-a Assay Detailed Review Paper , the Pig-a Assay Retrospective Validation Report , the draft OECD TG, the OECD Expert Working Group, as wells as the recent GTTC best practices Review Paper . (hesiglobal.org)

We utilize neurochemical, electrophysiological, and behavioral assays to interrogate the neural systems thought to be abnormal in neurodevelopmental disorders. (libd.org)
For advanced mechanistic and functional studies of drug effects, novel state-of-the-art platforms have recently been established, such large-scale chronic electrophysiological recordings from multiple regions of the rodent brain. (lu.se)

[ 7 ] Disease is more common in the fall and winter months (when rodents seek shelter indoors), and depends on rodent populations. (medscape.com)

Genes from the virus, found in lung tissue, were present in several different rodent species besides deer mice, meaning it is more ubiquitous in the environment than previously believed. (unm.edu)
In research published online April 28 in Nature Methods , the scientists report that the presence of male experimenters produced a stress response in mice and rats equivalent to that caused by restraining the rodents for 15 minutes in a tube or forcing them to swim for three minutes. (eurekalert.org)
Human hair removal brands such as Nair™ are sometimes used in research to remove hair from mice before surgery, imaging, and other procedures.1,3 While the use of these depilatory creams in rodent research is relatively. (awionline.org)

Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition. (immunochemistry.com)
Caspase 8 Colorimetric Activity Assay Kit, IETD Caspase-8 Colorimetric Activity Assay Kits provide a simple & convenient means for assaying the activity of caspases that recognize the sequence IETD. (sigmaaldrich.com)

It is assumed that humans usually become infected with hantaviruses and arenaviruses by inhalation of aerosolized droplets of urine, saliva, or respiratory secretions from infected rodents. (cdc.gov)

For instance, a dermatologist who consults for a wide range of prominent sunscreen companies was quoted as saying that it was "very premature to even cast doubt about the safety of this chemical," on grounds that rodent studies are not applicable to humans. (ewg.org)
Transmission to humans is through inhalation of aerosols of rodent excreta or, rarely through rodent bites. (msdmanuals.com)

A related experimental study involving rodents was conducted to establish if tolerance to peanut could be induced by oral administration (i.e. if peanut could act as a tolerogen). (usda.gov)
For the experimental rodent studies, peanut was administered directly by gavage to non-pregnant animals and lactating dams. (usda.gov)

Olfactory exposure to males, including men, causes stress and related analgesia in rodents", Robert E. Sorge, et al, Advance Online Publication on Nature Methods ' website 28 April 2014. (eurekalert.org)
CDC recently published recommendations to assist residents of the endemic area in avoiding exposure to rodents (8). (cdc.gov)

Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay. (abcam.com)

Current manufacturers of human growth hormone assay for potency by using hypophysectomized rodents. (genengnews.com)
Works in diverse cell lines: human, rodent, Drosophila. (immunochemistry.com)
John Quackenbush, Ph.D., a scientist at TIGR and one of the inaugural PGA directors, received funding for Expression Profiling of Rodent Models of Human Disease, selected as one of the nation's first PGAs. (jcvi.org)
2-Nitrotoluene was genotoxic in a range of in vitro and in vivo assays, was notably clastogenic in human peripheral lymphocytes and formed DNA adducts in exposed rodents. (gc.ca)

The first part of this paper reviews the effects of homeopathic remedies on several models of anxiety-like behaviours developed and described in rodents. (thieme-connect.com)
We evaluated whether siRNA targeting maternal hepatic angiotensinogen (Agt) could ameliorate symptoms of preeclampsia without adverse placental or fetal effects in 2 rodent models. (jci.org)
Thus, an RNAi therapeutic targeting Agt ameliorated the clinical sequelae and improved fetal outcomes in 2 rodent models of preeclampsia. (jci.org)
Performance evaluation of cytometric bead assays for the measurement of lung cytokines in two rodent models. (cdc.gov)
Our laboratory uses rodent models to determine how these risk factors modulate neurobiology and behavior. (libd.org)

Background: Conventional methods for individually housing, training, and testing rodents in behavioral assays can impose constraints that may limit some kinds of experimental external validity, preempt environmental enrichment, impose heavy experimenter time burdens that limit. (awionline.org)

2022. Performance and consistency of circulating warm water blankets for rodents. (awionline.org)

Precise oral dosing in rodents is usually achieved by intragastric gavage. (awionline.org)

Lassa fe- Lassa virus in many more districts and states in en- ver is endemic in West Africa and has been reported demic countries of the West African sub-region and from Sierra Leone, Guinea, Liberia, and Nigeria4-7. (folkhalsomyndigheten.se)

We also conduct research focused on developing novel behavioral assays with increased translational value for screening novel therapeutics. (libd.org)

C, D) No difference between PRCre and control females in sniffing WT males during assays of female sexual behavior. (cdc.gov)

Complex IV Rodent Enzyme Activity Microplate Assay Kit (ab109911) is used to determine the activity of cytochrome c oxidase in a mouse sample with speed and simplicity. (abcam.com)
describes assembly and curation of a data set of results for the Hershberger and other in vivo assays for AR activity. (nih.gov)

Skills, and Abilities**:* The incumbent must be familiar with basic laboratory techniques, and experience with behavioral testing using rodents is preferred. (umd.edu)

Now, an international team of pain researchers led by scientists at McGill University in Montreal may have uncovered one important factor behind this vexing problem: the gender of the experimenters has a big impact on the stress levels of rodents, which are widely used in preclinical studies. (eurekalert.org)

This assay is not only extremely expensive but also produces inconsistent results, according to Prolor Biotech. (genengnews.com)
Scientists whisper to each other at conferences that their rodent research subjects appear to be aware of their presence, and that this might affect the results of experiments, but this has never been directly demonstrated until now," says Jeffrey Mogil, a psychology professor at McGill and senior author of the paper. (eurekalert.org)

Development of a PC12 Cell Based Assay for Screening Catechol- O -methyltransferase Inhibitors. (libd.org)
possibility is that the damage is not induces mutations in a mutation integrated viral genome ( Todaro and repaired at al , and when the cell rep- assay permits it to be classified as Huebner, 1972) , and alteration of im- licates, the DNA polymerase correct- mutagenic. (who.int)
More specifically, data generated from rodent and cell- ing several days. (who.int)
2000). In *Correspondence to: Perrine Barraud, Department of Veterinary Medi- contrast, several cell surface markers have been used to cine, Neurosciences, Madingley Road, Cambridge CB3 OES, United enrich for NSCs in the rodent CNS. (lu.se)

The AR model is potentially a rapid, cost-effective replacement for the in vivo Hershberger assay ( EPA OPPTS 890.1400 ). (nih.gov)
describes use of the reference chemicals identified through the data curation effort to interrogate the performance of a ToxCast/Tox21 AR model based on 11 high-throughput assays. (nih.gov)
Testing vision in rodents: we have recently set-up behavioral procedures to test visual acuity in rodents by using the water maze task and validated it in an animal model of Parkinsonian eyes (Marrocco et al Scientific Reports 2019). (tigem.it)

These persons include mammalogists, wildlife biologists, scientists whose research concerns the ecology of rodent-borne zoonoses, and pest control operators. (cdc.gov)
The research team, which included pain experts from Haverford College and the Karolinska Institutet in Sweden and a chemosensory expert from Université de Montreal, found that the effect of male experimenters on the rodents' stress levels was due to smell. (eurekalert.org)
General anesthesia as used for rodent research can have adverse effects on physiologic mechanisms. (awionline.org)

This study provides evidence that murine acid ceramidase (Ac) is required for normal erythropoiesis and the development of rodent malaria. (elifesciences.org)
The DPD molecular diagnostic parasitology lab, which I lead, continues to collaborate on testing of environmental samples and the development of methods to evaluate the effectiveness of interventions to eliminate infected rodents and mollusks. (cdc.gov)

Induction of SRY box transcription factor 9 (SOX9) has been shown to occur in response to kidney injury in rodents, where SOX9-positive cells proliferate and regenerate the proximal tubules of injured kidneys. (lu.se)

2021. An apparatus for automatically training and collecting individualized behavioral data with socially housed rodents. (awionline.org)

All samples were found positive by a specific ompAPCR assay, confirming the close association of this Rickettsia species with X. ramesis in all its life stages. (datadryad.org)

Review the mitochondrial assay guide , or the full metabolism assay guide to learn about more assays for metabolites, metabolic enzymes, mitochondrial function, and oxidative stress, and also how to assay metabolic function in live cells using your plate reader. (abcam.com)

Rodent) will include publications relating to all the items in that category (e.g. (awionline.org)

These chemosignals signal to rodents the presence of nearby male animals. (eurekalert.org)

The kit contains sufficient materials for 30-100 assays depending assay setup and includes reagents for positive and negative controls. (cytoskeleton.com)

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