• RNA degradation by RNase serves as a defense mechanism against RNA viruses, provides the machinery for RNAi through the activity of the Dicer and Drosha enzymes, and has been recently targeted as a promising chemotherapeutic agent. (kenyon.edu)
  • Intracellular RNAs that are not targeted for degradation are protected from RNase activity by a number of different mechanisms. (kenyon.edu)
  • Lack of removal of many chemical inhibitors, dilution rather than concentration, and lack of removal or inactivation of RNases limit this approach from more general use, but where applicable in a specific context it's simple, cheap, fast, and effective. (thefreedictionary.com)
  • Recent data that has been gathered using several temperature-sensitive RNase MRP mutants that showed that inactivation of RNase MRP leading to severe reduction of the abundance of all early intermediates in the typical rRNA processing pathway. (wikipedia.org)
  • This thermostable nuclease exhibits the same enzymatic properties as E. coli RNase H, but is active at much higher temperatures. (neb.com)
  • RNase inhibitors are commonly used as a precautionary measure in enzymatic manipulations of RNA to inhibit and control for such contaminants. (thermofisher.com)
  • RNase Blaster decontaminates laboratory surfaces, equipment, and reaction containers (plastic tubes, glassware, etc.) of all RNase activity without inhibiting subsequent enzymatic reactions. (clontech.com)
  • The chimeric S-RNases retained enzymatic activity and were expressed at levels comparable to control transformants expressing SA2- and SC10-RNases. (nih.gov)
  • RNase III is a global regulator of gene expression in Escherichia coli that is instrumental in the maturation of ribosomal and other structural RNAs. (nih.gov)
  • Back in the 1970s, the groups of I. Kerr and P. Lengyel discovered a cellular endoribonuclease (RNase) activity that was increased by IFN and depended on the presence of double-stranded RNA (dsRNA) (Brown et al. (springer.com)
  • The RNase III motifs of KREPB1, KREPB2, and KREPB3 all conserve the amino acids that are critical to endonuclease function ( 13 ). (pnas.org)
  • 1977 ). Further, a correlation was found between this RNase activity and the synthesis of unusual 2′-5′ oligoadenylates (2-5A) (Fig. 1 ) by a family of enzymes called oligoadenylate synthetases (OAS) [(Baglioni et al. (springer.com)
  • One of the best-described IFN-dependent antiviral responses is the OAS/RNase L pathway. (springer.com)
  • Cheney hypothesizes that CFS moves through three distinct stages that begin when an intracellular organism activates the RNase L pathway. (thefreedictionary.com)
  • An E. coli strain carrying a codon optimized plasmid encoding RNase H from the extreme thermophile Thermus thermophilus . (neb.com)
  • hi guys im facing difficulty in preparing rnase that works properly .i use the protocol in molecular cloning ,sambrook ,but my rnase doesnt work whatso ever time i try.is there any other method to prepare rnase solution from powder form. (protocol-online.org)
  • The roles of RNase-L in antimicrobial immunity and the cytoskeleton-associated innate response. (springer.com)
  • RNase MRP (also called RMRP) is an enzymatically active ribonucleoprotein with two distinct roles in eukaryotes. (wikipedia.org)
  • Further research in Yeast cell RNase MRP has shown a potential role in the regulation of the cell cycle. (wikipedia.org)
  • Parniak, Professor of Molecular Genetics & Biochemistry at the University of Pittsburgh and Principal Investigator of this multi-team research effort, has nearly 20 years experience in HIV research and is regarded as a thought leader in the discovery of drugs targeting HIV RNase H. (thefreedictionary.com)
  • NOTE: The RNAse H Reaction Buffer contains MgCl 2 . (neb.com)
  • RNases do not have specific reaction buffer needs: they are active in pure water and in the presence of Tris or NaCl. (sigmaaldrich.com)
  • Arg91 has the greatest influence on the stability of the hRI:RNase 1 complex, 2.7 kcal/mol. (kenyon.edu)
  • The fact that there is not often a mutation in the promoter region in both alleles shows the lethality of not having this RNA present that is transcribed by RNAse MRP. (wikipedia.org)
  • Other RNases are active at the cellular level, for instance in modifying transfer RNA and ribosomal RNA after transcription. (encyclopedia.com)
  • In mammalian cells, the distinct RNase angiogenin appears to provide the former function, but the activity-independent effects may be related to the tumor-suppressive effects of Rny1's ortholog, RNASET2. (sciencemag.org)
  • RNase L attenuates mitogen-stimulated gene expression via transcriptional and post-transcriptional mechanisms to limit the proliferative response. (springer.com)
  • Gusho E, Baskar D, Banerjee S. New advances in our understanding of the "unique" RNase L in host pathogen interaction and immune signaling. (springer.com)
  • Hydrogen bonding of Lys41 with hRI is the strongest and most conserved interaction between hRI and other RNase variants. (kenyon.edu)
  • It appears that the recognition function of S-RNase is not localized to a specific domain. (nih.gov)