• The unspliced human immunodeficiency virus type 1 (HIV-1) RNAs are translated as Gag and Gag-Pol polyproteins or packaged as genomes into viral particles. (sigmaaldrich.com)
  • However, the function of Rev to modulate the silencing sites of unspliced RNAs would be advantageous for the efficient translation that is required to support protein production prior to viral packaging and particle production. (sigmaaldrich.com)
  • Packaging also requires nuclear export of viral genomes, and their cytoplasmic transport toward the plasma membrane. (cnrs.fr)
  • We show that eukaryotic initiation factor 5A (eIF-5A) is essential for Rev and Rev-mediated viral RNA export, but not for nuclear export of CTE RNA. (rupress.org)
  • Rev is a nucleocytoplasmic shuttle protein that directly binds to its Rev-response element (RRE) RNA target sequence, which is part of all unspliced and incompletely spliced viral mRNAs. (rupress.org)
  • We compared two different blood RNA collection tube systems -- the PAXgeneTM Blood RNA system and the TempusTM Blood RNA system - and assessed the effects of suboptimal blood volumes in collection tubes and of transportation of blood samples by standard mail. (technologynetworks.com)
  • Paraspeckles are nuclear bodies built on the long noncoding RNA, NEAT1, that regulate cellular homeostasis, but how they sense and help under stress is unclear. (nature.com)
  • Fischer U, Huber J, Boelens WC, Mattaj IW and Lührmann R (1995) The HIV‐1 Rev activation domain is a nuclear export signal that accesses an export pathway used by specific cellular RNAs. (els.net)
  • An accelerated rate of glucose transport is among the most characteristic biochemical markers of cellular transformation. (sciencemag.org)
  • The virus that most closely matched H5N1 for virulence was one with the hemagglutinin (HA), the neuraminidase (NA) and the PB1 avian flu virus RNA molecules with their genes combined with the remaining five RNA molecules (PB2, PA, NP, M, and NS) with their genes from the human flu virus. (wikipedia.org)
  • however, little is known about other components of the transport machinery or how these components recognize specific RNA cargoes. (nih.gov)
  • Both filamentous NPC-attached appendages are thought to provide docking sites and guiding structures for transport cargoes before their translocation through the central pore channel. (rupress.org)
  • When RNA is desired for downstream processes to use a quick 7 minute procedure allows for purification of RNA. (omegabiotek.com)
  • RNA was re-purified using RNA Transport purification method, which takes less than 5 minutes per sample set. (omegabiotek.com)
  • Total RNA was purified from cultured cells using the E.Z.N.A.® Total RNA Kit I. 4 µg of RNA was aliquoted to individual tubes containing RNA Transport, then stored at room temperature for the indicated dats. (omegabiotek.com)
  • High-quality RNA could be extracted from blood samples stabilized with both PAXgene and Tempus tubes. (technologynetworks.com)
  • The RNA yields obtained from the blood samples collected in Tempus tubes were consistently higher than from PAXgene tubes. (technologynetworks.com)
  • Higher RNA yields were obtained from cord blood (3 -- 4 times) compared to adult blood with both types of tubes. (technologynetworks.com)
  • Prior to translocation some nuclear retention (NR) factors are stripped from the RNA. (els.net)
  • Exposure of control cells to the tumor-promoting phorbol ester 12-O-tetradecanoyl phorbol-13-acetate (TPA) for 18 hours had a similar effect of increasing the rate of glucose transport and the abundance of transporter messenger RNA. (sciencemag.org)
  • Conserved signals and machinery for RNA transport in Drosophila oogenesis and embryogenesis. (nih.gov)
  • Stem-loops A and B are self-complementary and bind Gag in their dimeric form, while the C and D elements mediate loop-loop interactions that facilitate RNA dimerization. (cnrs.fr)
  • We show that the 50,000 samples collected in the MoBa biobank provide RNA of high quality and in sufficient amounts to allow gene expression analyses for studying the association of disease with altered patterns of gene expression. (technologynetworks.com)
  • It turns out that Staufen2 recognizes its target RNAs in a much more complex way than previously thought," adds Dr. Simone Heber, lead author of the article and postdoc at the STB. (idw-online.de)
  • In addition, it is a key factor in the transport of RNA to synapses and is therefore important for synaptic plasticity, the basis of memory and memory formation. (idw-online.de)
  • This suggests that RNA dimerization is essential for vesicular transport, consistent with its proposed requirement for Gag binding. (cnrs.fr)
  • The RNA transport element RTE is essential for IAP LTR-retrotransposon mobility. (semanticscholar.org)
  • GO annotations related to this gene include poly(A) RNA binding and ribonuclease MRP activity . (genecards.org)
  • Knowledge of the function of Staufen2 contributes significantly to our overall understanding of RNA transport in higher organisms - a function that is also of great interest to stem cell researchers. (idw-online.de)
  • The HA RNA molecule contains the HA gene, which codes for hemagglutinin, which is an antigenic glycoprotein found on the surface of the influenza viruses and is responsible for binding the virus to the cell that is being infected. (wikipedia.org)
  • Previous studies had suggested that only two RNA binding domains in Staufen2, namely dsRBD 3 and 4, are responsible for binding. (idw-online.de)