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  • tRNAs
  • Consistent with the cleavage of tRNAs Arg , the RNA fraction of colicin-treated cells significantly reduced the amino acid-accepting activity only for arginine. (pnas.org)
  • nascent
  • Rho binds to the nascent RNA and translocates 5′ to 3′ along RNA using energy derived from ATP hydrolysis ( 4 ). (pnas.org)
  • Before this information can be relayed to the cytoplasm, the nascent RNA undergoes extensive post-transcriptional processing before arriving at its final destination in cells. (mdpi.com)
  • hairpin
  • The biological function of the hairpin ribozyme is to cleave concatenated transcripts to unit length by action of an RNA-mediated, site-specific phosphodiester bond cleavage reaction within its cognate substrate. (grantome.com)
  • Recent kinetic analyses of A38 and G8 abasic hairpin ribozyme variants suggested that catalysis of the native RNA does not proceed through a general acid/base mechanism using A38 and G8 (as previously proposed), but instead uses transition-state stabilization involving electrostatic contributions from specific function groups contributed by the exogenously added rescue bases. (grantome.com)
  • For the current proposal, we have generated crystals of a minimal all-RNA hairpin ribozyme in complex with a transition-state mimic (vanadate) that should reveal additional, putative specific acid/base catalysts at high resolution. (grantome.com)
  • localization
  • In some experiments, confocal immunolabeling of wholemount preparations was used to examine the localization of specific mechanosensitive TRP channels in retinal vascular smooth muscle cells (VSMCs). (arvojournals.org)
  • gene expression
  • Two recent papers from Pascale Cossart and colleagues [ 6 , 7 ] present a comprehensive microarray analysis of the trans-criptome of Listeria monocytogenes in different conditions, uncovering an unsuspected variety of regulatory roles for noncoding RNAs in controlling changes in gene expression that characterize the transition from saprophytic to pathogenic lifestyle. (biomedcentral.com)
  • enzyme
  • Indoleamine 2,3-dioxygenase has been identified as an enzyme endowed with powerful immunomodulatory effects, resulting from its enzymatic activity that leads to catabolism of the essential amino acid l -tryptophan (L-TRP) ( 1 , 2 ). (jimmunol.org)
  • genome
  • the other those encoded in trans from their target gene, which are often located at completely unrelated positions on the genome, such as the canonical small RNAs (sRNA). (biomedcentral.com)
  • keratinocytes
  • In melanosomes, specialized intracellular organelles of melanocytes, melanin is produced by sequential enzymatic processes and is then moved along the melanocyte dendrites and transferred to adjacent keratinocytes for photo-protection [ 3 , 4 ]. (mdpi.com)
  • Skin color is determined by the production of melanin and the distribution of melanosomes transferred by each melanocyte to its neighboring keratinocytes [ 3 ]. (mdpi.com)
  • Melanosomes are transported to dendrite tips of melanocytes and are transferred to surrounding keratinocytes ( Jimbow and Sugiyama 1998 ). (rupress.org)
  • channels
  • In the present study we sought to determine the potential role of various mechanosensitive TRP channels to myogenic signaling in arterioles of the rat retina. (arvojournals.org)
  • Our results suggest that retinal VSMCs express a range of mechanosensitive TRP channels, but only TRPV2 appears to contribute to myogenic signaling in this vascular bed. (arvojournals.org)
  • role
  • For example, water has been observed in the 50S ribosome peptidyl transfer site, but there is no direct evidence that solvent plays an essential role in catalysis. (grantome.com)
  • RNA-Seq corroborates a primary itch afferent role for NPPB in mouse and potentially NPPB and NPPA in rats and humans, but does not support GRP as a primary itch neurotransmitter in mouse, rat, or humans. (biomedcentral.com)
  • examine
  • Here we used next-gen RNA-Seq to examine the expression of transcripts coding for GRP, NPPB, NMB, and other peptides in DRG, trigeminal ganglion, and the spinal cord as well as expression levels for their cognate receptors in these tissues. (biomedcentral.com)
  • amino
  • In 1993, Koonin and Rudd predicted the spoU gene product to be an RNA methyltransferase based on the similarity of its deduced amino acid sequence to that of Streptomyces azureus Tsr [ 1 ], which catalyzes methylation of the ribose of adenosine (A) at position 1067 in 23S rRNA to form 2'- O -methyladenosine (Am1067) [ 4 ]. (pubmedcentralcanada.ca)
  • Horizontal
  • We have used this annotated assembly to investigate the genetic basis of a range of traits critical to the evolution of the Hexapoda, the phylogenetic position of H. duospinosa and potential horizontal gene transfer events. (biomedcentral.com)
  • Our detection of candidate horizontal gene transfer candidates confirms that this phenomenon is occurring across Collembola. (biomedcentral.com)
  • structural
  • The goal of this study is to elucidate the structural features leading to the selection of the RNA editing site by the protein ADAR1, which is essential to complete the viral life cycle. (grantome.com)
  • catalyzes
  • TRP-2 acts as a dopachrome tautomerase and catalyzes a quicker conversion of dopachrome to 5,6-dihydroxyindol-2-carboxylic acid (DHICA) [ 8 ]. (mdpi.com)
  • Figure
  • They reported that the three RNA 2'- O -methyltransferases have three conserved motifs, termed motifs 1, 2 and 3 ( Figure 3 ) [ 2 ]. (pubmedcentralcanada.ca)
  • formation
  • TRP-1 increases the eumelanin/pheomelanin ratio, facilitating the formation of carboxyl group-containing DHICA oxidase eumelanins [ 7 ]. (mdpi.com)
  • site
  • corticotropin of human stimuli with RNA or DNA chains found in an site of STAT6 with STING. (erik-mill.de)
  • affect
  • These two mutations were localized in two conserved regions of the tRNA(Trp) and affect, respectively, the D-stem and the T-stem of the mitochondrial tRNA leading to a disruption of the secondary structure of this tRNA. (lu.se)
  • function
  • Thus, the N-terminal 45% region of colicin D, which is highly homologous to that of colicin B, is thought to function in receptor binding and membrane transfer. (pnas.org)
  • variety
  • Oligos can be produced to contain any desired DNA or RNA sequence and can be prepared to include a wide variety of base and sugar modifications. (jove.com)