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  • endogenous
  • Long ncRNAs may as well be produced from transcriptionally active pseudogenes, and can in turn yield endogenous short interfering RNAs (siRNAs) or miRNAs. (prolekare.cz)
  • We demonstrated that lnc-ISG20 functions as a competitive endogenous RNA to bind miR-326 to reduce its inhibition of ISG20 translation. (asm.org)
  • Our studies indicate that lnc-ISG20 functions as a competing endogenous RNA that binds miR-326 and reduces its inhibitory effect on ISG20. (asm.org)
  • Northerns
  • While probes for Northerns and Southerns have been historically synthesized by random-primed labeling, our results indicate that probes synthesized by asymmetric PCR are 3-5 fold more sensitive than random-primed probes, and that RNA probes provide an additional 10-fold increase in sensitivity. (thermofisher.com)
  • Gene
  • TRG-TCC2-3 (Transfer RNA-Gly (TCC) 2-3) is an RNA Gene, and is affiliated with the non-coding RNA class. (genecards.org)
  • Reconstruction of genome evolution in Mollicutes revealed that, beside many gene losses, occasional gains by horizontal gene transfer also occurred. (plos.org)
  • Here, we demonstrate, using RNA interference, that the TFIIS4 gene encoding a development-specific homolog of RNA polymerase II elongation factor TFIIS, is indispensable for ncRNA synthesis in the new somatic nucleus. (prolekare.cz)
  • Regulatory short micro-RNAs (miRNAs) or longer ncRNAs can be transcribed from gene introns. (prolekare.cz)
  • Normal Amino Acid Sequence Ile Ile Phe Gly Val 506 507 508 509 510 Sally's Gene Segment DNA ATC ATT GGT GTT ACT m-RNA UAG UAA CCA CAA UGA t-RNA Amino acid Does Sally have cystic fibrosis? (studyres.com)
  • Epigenetic mechanisms modulate gene expression by altering modifications of DNA or histones and via noncoding RNAs. (pnas.org)
  • Probe
  • The RNA is then transferred to a membrane, crosslinked and hybridized with a labeled probe. (thermofisher.com)
  • 1 µl of each test solution was added to 9 µl of water containing 1 ng of a 5 x 104 cpm single-stranded RNA probe and incubated overnight at 37°C. 5 µl of each reaction were electrophoresed on a 5% acrylamide/8 M urea gel and exposed to film overnight. (thermofisher.com)
  • tissues
  • Fresh brain and kidney tissues were dissected and immediately submerged in 5 volumes of RNAlater, incubated at 4°C overnight and then transferred to -20°C. Thirteen months later, the RNA later treated samples were thawed at room temperature, about 50 mg of tissue was collected, and the remainder of the samples were refrozen. (thermofisher.com)
  • integrity
  • The Northern blotting procedure is straightforward and provides opportunities to evaluate progress at various points (e.g., integrity of the RNA sample and how efficiently it has transferred to the membrane). (thermofisher.com)
  • In addition to being a quantitative tool, Northern analysis has the advantage of being the only method able to simultaneously provide information about the size, alternative splicing and integrity of RNA samples. (thermofisher.com)
  • sensitivity
  • Second, a standard Northern procedure is, in general, less sensitive than nuclease protection assays and RT-PCR, although improvements in sensitivity can be achieved by using high specific activity antisense RNA probes, optimized hybridization buffers and positively charged nylon membranes. (thermofisher.com)
  • Inhibition
  • Inhibition of vascular endothelial growth factor A (VEGF) in RKO colon cancer cells transfected with anti-VEGF short interfering RNA (siRNA). (jamanetwork.com)
  • membrane
  • Thus, the N-terminal 45% region of colicin D, which is highly homologous to that of colicin B, is thought to function in receptor binding and membrane transfer. (pnas.org)
  • demonstrate
  • These results demonstrate that RNAlater is an efficient sample collection/stabilization reagent for protecting RNA, and that RNAlater-treated samples can be used for routine protein analysis such as Western blotting. (thermofisher.com)
  • cells
  • The solution permeates the cells, stabilizing the RNA inside them. (thermofisher.com)
  • The RKO cells treated with various doses of anti-VEGF VP short interfering RNA (siRNA) demonstrated decreased proliferation when compared with untransfected controls (UT). (jamanetwork.com)
  • gly misinsertion occurs in recA- cells and, therefore, the requirement for recA is 'downstream' of mistranslation. (bcm.edu)
  • Colicins E4 to E6 quickly stop amino acid incorporation in treated cells ( 6 ), suggesting impairment of protein synthesis analogous in the established case of E3, which specifically cleaves 16S-RNA at the 49th bond from the 3′ end leading to inactivation of ribosomes (7- 9). (pnas.org)
  • To identify influenza A virus (IAV)-related lncRNAs, we performed RNA deep sequencing to compare the profiles of lncRNAs in A549 and HEK293T cells with or without IAV infection. (asm.org)
  • analysis
  • Obtaining high quality, intact RNA is a critical step in performing Northern analysis. (thermofisher.com)
  • Analysis of three independent insertional mutants of Arabidopsis ETFQO revealed a dramatic reduction in their ability to withstand extended darkness, resulting in senescence and death within 10 d after transfer, whereas wild-type plants remained viable for at least 15 d. (plantcell.org)
  • procedure
  • This step takes only 2 hr, and it can trim almost an entire day off the standard procedure, which generally requires an overnight transfer. (thermofisher.com)
  • mammals
  • Moreover, piwi-interacting RNAs (piRNAs), in Drosophila as in mammals, are derived from heterochromatin domains containing mostly transposable elements and degenerate transposons, and are involved in transposon silencing. (prolekare.cz)
  • shown
  • Different types of non-coding RNAs (ncRNAs) were shown to be implicated in the programmed DNA elimination process that occurs in this organism. (prolekare.cz)
  • messenger
  • Each group of three adjacent bases in the messenger RNA attracts a transfer RNA containing a complementary sequence of three bases. (retzepti.ru)
  • The cfCRF-R1 messenger RNA is highly expressed in the brain, and its distribution pattern correlates well with that of mammalian CRF-R1, except for weak expression in the pituitary. (embl-heidelberg.de)
  • Cellular
  • Long noncoding RNAs (lncRNAs) are involved in many aspects of cellular processes, including the antiviral immune response. (asm.org)
  • specific
  • The 5′-leader ribozyme recognizes a specific amino acid and self-charges to the 3′-end of RNA. (nih.gov)
  • samples
  • RNA late r® is an aqueous, nontoxic, tissue and cell collection reagent that stabilizes and protects RNA in intact, unfrozen tissue and cell samples. (thermofisher.com)
  • Samples were either kept frozen at -80ºC, or stored in RNA later ® at 4ºC. (thermofisher.com)
  • First, if RNA samples are even slightly degraded, the quality of the data and the ability to quantitate expression are severely compromised. (thermofisher.com)
  • Although
  • Most ncRNA production has been attributed to RNA polymerase II, although miRNAs are also transcribed by RNA polymerase III [ 4 ]. (prolekare.cz)
  • short
  • Cell cycle effects of treatment with anti-vascular endothelial growth factor (VEGF) VP short-interfering RNA (siRNA). (jamanetwork.com)
  • mouse
  • RNA and protein were isolated with the PARIS™ Kit from ~50 mg of mouse brain or kidney stored for 13 months at -20°C in RNAlater. (thermofisher.com)