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  • genome
  • Subsequently, in a poorly understood process, the viral RNA genome is transferred directly across an endosomal membrane, and into the host cell cytoplasm, to initiate infection. (asm.org)
  • Origin
  • The above data have led us to question the cellular origin of axonal and presynaptic RNAs, often located at considerable distances from the neuronal soma. (thefreelibrary.com)
  • vitro
  • Even though such RNA enzymes so far are not identified from known organisms, in vitro selection has generated such RNA catalysts from a pool of random RNA sequences. (nih.gov)
  • d ) Doped RNA pool for in vitro selection of the second generation of prototype flexizyme. (nih.gov)
  • f ) Doped RNA pool for in vitro selection of dFx, eFx and aFx that recognize aromatic leaving groups. (nih.gov)
  • NaCl
  • I use upward capillary transfer with NaCl/NaOH-containing transfer buffer, stack of paper towels and 400g weight above it. (protocol-online.org)
  • The temperature dependence of the areas under the proton magnetic resonance spectra of unfractionated yeast transfer RNA in 1.0 molar NaCl is a consequence of salt-induced aggregation and does not constitute a monitor of the melting of secondary molecular structure. (sciencemag.org)
  • membrane
  • After overnight transfer of RNA from formaldehyde-containing agarose gel to the nitrocellulose membrane I always observe a "blind" spot at the middle of membrane with bromfenol blue lanes at the edges. (protocol-online.org)
  • It means that the transfer of RNA samples loaded to the central gel pockets is not so effective as for "side" samples (it was confirmed both by DIG-labeling system and using of radiolabeled probes: samples at the edges of membrane looked much brighter than samples loaded to the 3-4 pockets located at the middle of gel). (protocol-online.org)
  • specific
  • The 5′-leader ribozyme recognizes a specific amino acid and self-charges to the 3′-end of RNA. (nih.gov)
  • form
  • In total, 651 subtomographic volumes were aligned, classified, and averaged to obtain detailed pictures, showing both the conversion of virus into its expanded form and the passage of RNA into intact liposomes. (asm.org)