Rna transcription addition localization of specific rnas. Medical search. Frequent questions

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). (wikipedia.org)
As a largest plant-specific transcription factor family, NAC domain proteins play an important role in plant development and regulation of abiotic stress tolerance. (frontiersin.org)
In addition, NAC domain proteins have become a popular research topic for different plant species. (frontiersin.org)
Shi D, Cheng X, Saquet A, Grifone R. RNA-Binding Proteins and Inner Ear Hair Cell. (encyclopedia.pub)
RNA-Binding Proteins and Inner Ear Hair Cell" Encyclopedia , https://encyclopedia.pub/entry/30958 (accessed December 05, 2023). (encyclopedia.pub)
RNA-binding proteins (RBPs) regulate gene expression at the post-transcriptional level. (encyclopedia.pub)
LIN28 (LIN28A and LIN28B) proteins are highly conserved small cytoplasmic RNA-binding proteins (RBPs) that function as pluripotency factors, regulating the transition from self-renewal to a differentiated cell fate [ 1 ] . (encyclopedia.pub)
Here, we study a collection of 75 CLIP-Seq experiments mapping the RNA binding sites for a diverse set of 51 human proteins to explore the role of TEs in post-transcriptional regulation of human mRNAs and lncRNAs via RNA-protein interactions. (biomedcentral.com)
We detect widespread interactions between RNA binding proteins (RBPs) and many families of TE-derived sequence in the CLIP-Seq data. (biomedcentral.com)
Programmable DNA binding proteins have emerged as an exciting platform for engineering synthetic transcription factors for modulating endogenous gene expression 5 - 11 . (cdc.gov)
Among the established custom DNA binding domains, Cas9 is most easily scaled to facilitate genome-scale perturbations 3 , 4 due to its simplicity of programming relative to zinc finger proteins and transcription activator-like effectors (TALEs). (cdc.gov)

Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
Post-transcriptional regulation of human genes by TE-derived sequences has been observed in specific contexts, but has yet to be systematically and comprehensively investigated. (biomedcentral.com)
This protein forms a tetrameric complex with the alpha subunit, and stimulates transcription of target genes. (nih.gov)
Experimentally confirmed target genes were identified for the 73 diagnostic miRNAs, from which proliferation genes were selected from CRISPR-Cas9/RNA interference (RNAi) screening assays. (cdc.gov)
We use these engineered Cas9 activation complexes to investigate sgRNA targeting rules for effective transcriptional activation, demonstrate multiplexed activation of 10 genes simultaneously, and upregulate long intergenic non-coding RNA (lincRNA) transcripts. (cdc.gov)

SncRNAs are less than 200 nt in length, consisting of microRNAs (miRNAs), Piwi-interacting RNAs (piRNAs), endogenous small interfering RNAs (endo-siRNAs or esiRNAs) and transcription initiation RNAs (tiRNAs) [ 6 ]. (ijbs.com)
Further, we developed a pipeline to characterise novel genome-wide endogenous short interfering RNAs (endo-siRNAs). (uni-frankfurt.de)
Further, using RAPID, we investigated the aberrations of endo-siRNAs, and their respective transcriptomic alterations caused by an RNAi pathway triggered by feeding small RNAs against a target gene. (uni-frankfurt.de)

Circular RNAs (circRNAs) are closed, long, non-coding RNAs that are formed via direct reverse splicing of precursor mRNA that function by regulating RNA transcription and protein production, as well as sponging miRNAs ( 4 ). (spandidos-publications.com)
DEFB1 expression after transfection with two micro RNAs (miRNAs), hsa-miR-186-5p and hsa-miR-340-5p, provided evidence that DEFB1 expression could be modulated by these miRNAs and hsa-miR-186-5p had a higher binding efficiency with DEFB1. (cdc.gov)

The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs in the N-terminal domain which are pivotal for RNA specificity and binding. (wikipedia.org)
The protein also has a glycine rich arginine-glycine-glycine (RGG) region called the RGG box which enables protein and RNA binding. (wikipedia.org)
hnRNP A1 inhibits the binding of Rex protein to its response element in 3' long terminal repeat (LTR) of all viral RNAs. (wikipedia.org)
The CmNAC1-GFP fusion protein was transiently expressed in tobacco leaves for subcellular localization analysis, and we found that CmNAC1 is localized in the nucleus. (frontiersin.org)
Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
They play major roles in the tissue- and stage-specific expression of protein isoforms as well as in the maintenance of protein homeostasis. (encyclopedia.pub)
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a cellular metabolic protein, was found to colocalize with viral RNA-dependent RNA polymerase (NS5) in JEV-infected cells. (biomedcentral.com)
Numerous studies have shown that replication of RNA viruses is involved in many specific RNA-RNA, RNA-protein, and protein-protein interactions. (biomedcentral.com)
During the past twenty years, however, numerous studies indicated that GAPHD is a multifunction protein in addition to its traditional role in glycolysis [ 10 ]. (biomedcentral.com)
Using semi-quantitative reverse-transcription polymerase chain reaction (RT-PCR) and western blots experiments, we compared changes in ETS-1 and ETS-2 expression, their protein levels, and the regulation of some of their target gene expressions at different stages of the ocular tumoral progression in the transgenic mouse model, Tyrp-1-TAg, with those in normal eyes from control mice of the same age. (molvis.org)
We observe that each gene and its products have a unique set of DNA, RNA or protein motifs that encode a regulatory program to define the logical circuitry that guides the life cycle of these biomolecules, from transcription to degradation. (biomedcentral.com)
Our meta-analysis suggests a widespread role for TEs in shaping RNA-protein regulatory networks in the human genome. (biomedcentral.com)
It involves crosslinking cells to lock RNA-protein interactions, immunoprecipitating the complexes using an antibody specifically targeted to the RBP, sequencing cDNA reverse transcribed from the captured RNA, and statistically analyzing the aligned sequencing reads [ 26 ]. (biomedcentral.com)
This gene encodes the GA-binding protein transcription factor, beta subunit. (nih.gov)
Note: In August, 2008, the nomenclature of the GA binding protein transcription factors was clarified. (nih.gov)
Cas9 nuclease can be converted into an RNA-guided DNA binding protein (dCas9) via inactivation of its two catalytic domains 12 , 13 and then fused to transcription activation domains. (cdc.gov)

Although the current generation of dCas9-based transcription activators is able to achieve up-regulation of some endogenous loci, the magnitude of transcriptional up-regulation achieved by individual single-guide RNAs (sgRNAs) 12 typically ranges from low to ineffective 8 - 11 . (cdc.gov)

Circular RNAs (circRNAs), a novel type of non-coding RNAs (ncRNAs), are ubiquitously expressed in eukaryotic cells during post-transcriptional processes. (ijbs.com)

This study highlights the findings that infection of JEV changes subcellular localization of GAPDH suggesting that this metabolic enzyme may play a role in JEV replication. (biomedcentral.com)
Does a transcript get translated, stored, decayed or transported to a specific subcellular location? (biomedcentral.com)

It contains a single-stranded positive RNA genome of 10,976 nucleotides (nts) in length that encodes a single large open reading frame (ORF) flanked by a 5'-untranslated region (5'-UTR, 95 nts) and a 3'-untranslated region (3'-UTR, 585 nts). (biomedcentral.com)
To date, various genome-scale loss-of-function screening methods have been developed, including approaches employing RNA interference 1 , 2 and the RNA-guided endonuclease Cas9 from the microbial CRISPR (clustered regularly interspaced short palindromic repeat) adaptive immune system 3 , 4 . (cdc.gov)

Long noncoding RNAs (lncRNAs), due to their multifunction in various biological processes, have been indicated to play a crucial role in CSC renewal and stemness maintenance. (nature.com)

Many new nucleic acid-based diagnostic tools or assays have been developed that allow analysis of DNA and RNA molecules in clinical samples. (enzolifesciences.com)
DNA/RNA probe assays are faster and sensitive so that many conventional diagnostic tests for viruses and bacteria involving culturing of the organisms are being fast replaced by molecular probe assays. (enzolifesciences.com)
instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of JEV by electrophoretic mobility shift assays. (biomedcentral.com)

We also predicted phasing of siRNAs, which are regulated by the RNA interference (RNAi) pathway. (uni-frankfurt.de)

The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)

Notably, circRNAs display cell-type, tissue-type and developmental-stage specific expression patterns in eukaryotic transcriptome, which reveals their significant regulatory functions in gene expression. (ijbs.com)
However, it has been proven that more than 62% of genomic DNA serves as a template for transcription, which indicates that there are abundant non-coding RNAs (ncRNAs) in human transcriptome [ 2 , 3 ]. (ijbs.com)
We find that the small RNA transcriptome is altered, even if a gene unrelated to RNAi pathway is targeted. (uni-frankfurt.de)

We developed a generic automated eukaryotic short interfering RNA (siRNA) analysis tool, called RAPID. (uni-frankfurt.de)

In practice, double and single stranded DNAs, mRNAs, and other RNAs synthesized in vitro are all used as probes. (enzolifesciences.com)

Unlike linear RNAs, circRNAs form covalent-closed continuous loops without 5' to 3' polarities and poly (A) tails. (ijbs.com)
Circular RNAs (circRNAs), a novel member of the lncRNA kingdom, are generated by non-sequential backsplicing of exons, introns or a combination of both [ 7 - 9 ]. (ijbs.com)
CircRNAs were first identified in RNA viruses in the 1970s [ 12 - 14 ]. (ijbs.com)
Circular RNAs (circRNAs) are molecular sponges that are involved in regulation of multiple types of cancer. (spandidos-publications.com)

In addition, a previous study demonstrated that circRNA/miRNA/mRNA networks are involved in the development and progression of multiple types of malignancy ( 7 ). (spandidos-publications.com)
Thus, further investigations into the specific molecular mechanisms underlying circRNA/miRNA/mRNA regulation are required to aid discovery of effective therapeutic targets. (spandidos-publications.com)

Methylation of arginine residues in the RGG box may regulate RNA-binding activity. (wikipedia.org)
NAC transcription factors regulate the stress response in plants. (frontiersin.org)

Its M9 nuclear localisation sequence (NLS), a glycine rich region downstream from the RGG box, acts as both a nuclear localization and nuclear export signal. (wikipedia.org)
However, addition of O-GlcNAcylation (GlcNAc) moiety to serine or threonince is a common and reversible modification that impairs the protein's binding of karyopherin beta (Transportin-1) resulting in nuclear localization of hnRNPA1. (wikipedia.org)

hnRNP A1 is involved in the life cycle of DNA, positive sense RNA, and negative sense RNA viruses are multiple stages post-infection. (wikipedia.org)

hnRNP A1 has been found redistributed in the cytoplasmic site of viral replication bound to the 5' UTR region of the viral RNA, promoting synthesis of negative-strand RNA. (wikipedia.org)

Molecular probes can be broadly categorized into DNA probes and RNA probes, cDNA probes, and synthetic oligonucleotide probes. (enzolifesciences.com)

hnRNP A1's pro-viral effect is present in the Sindbis virus (a positive sense RNA virus) infection model. (wikipedia.org)

These variants have multiple alternative transcription initiation sites and multiple polyA sites. (wikipedia.org)

A DNA probe is a labeled fragment of DNA that contains a nucleotide sequence specific for the gene or chromosomal region of interest. (enzolifesciences.com)
Probes labeled by nick translation can be used in many different hybridization techniques including: chromogenic in situ hybridization (CISH), fluorescent in situ hybridization (FISH), screening gene banks by colony or plaque hybridization, DNA or RNA transfer hybridization, and re-association kinetic studies. (enzolifesciences.com)
This class of noncoding RNAs is small, single stranded, and 19-25 nucleotide long that act as negative regulators involved in posttranscriptional silencing of the gene expression [ 5 ]. (hindawi.com)
The life of a gene product, from transcription to degradation, is controlled by a series of regulatory decisions. (biomedcentral.com)

This affinity and complementary sequence allow binding to specific regions of a target sequence of nucleotides. (enzolifesciences.com)

Moreover, expression levels of DEGs were verified in HNSCC cells and tissues using reverse transcription‑quantitative (RT‑q)PCR. (spandidos-publications.com)

Transactivation assay in yeast cells revealed that CmNAC1 functions as a transcription activator, and its transactivation domain is located in the C-terminus. (frontiersin.org)
In a PCR array analysis of 84 transcription factors, either overexpressing DEFB1 or siRNA silencing of DEFB1 expression significantly modulated the expression of signal transducer and activator of transcription 3 (STAT3). (cdc.gov)

We identified well positioned nucleosomes shifted downstream of the transcription start site. (uni-frankfurt.de)

Further, alignment coverage peaks on specific positions of the TE consensus sequences, illuminating a diversity of TE-specific RBP binding motifs. (biomedcentral.com)

But what this really boils down to is recognition that, in addition to selection, drift, mutation and other established evolutionary processes, other factors, particularly developmental influences, shape the evolutionary process in important ways. (uncommondescent.com)

The role of two members of the ETS (E26 avian leukemia oncogene) family of transcription factors, ETS-1 and ETS-2, has been investigated in many cancers but has not yet been studied in ocular tumors. (molvis.org)

Lnc408 can recruit transcript factor SP3 to CBY1 promoter to serve as an inhibitor in CBY1 transcription in BCSCs. (nature.com)
In addition, let-7a plays an important role in BCSCs' self-renewal by inhibiting the expression of H-Ras [11, 12]. (researchsquare.com)

Our tool captures diverse siRNA characteristics from small RNA sequencing data and provides easily navigable visualisations. (uni-frankfurt.de)

In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered in cells overexpressing DEFB1, suggesting this to be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)

The NAC domain transcription factors in Arabidopsis function in plant development, senescence, and stress regulation. (frontiersin.org)
It is relatively well documented that transcription factors and signaling pathways are critically involved in the formation of inner ear structures and in the development of hair cells. (encyclopedia.pub)

Our goal is to convey a picture of pattern formation that draws attention to the principles rather than solely to specific molecular mechanisms. (mpi-cbg.de)

Nucleic acid probes are either a single stranded DNA or RNA with a strong affinity towards a specific DNA or RNA target sequence. (enzolifesciences.com)

In addition, Lin28B functions to enhance the regenerative competence of maturing supporting cells in the cochlea through cooperation with Follistatin, which inhibits Lin28B-induced TGF-ß signaling that can trigger proliferative quiescence [ 5 ] . (encyclopedia.pub)

In the case of Hepatitis C virus (HCV), a positive sense RNA virus, hnRNP A1 interacts with a crucial region near the 3' end of the virus' open reading frame (ORF) called the cis-acting replication element. (wikipedia.org)

In addition, your package will include a detailed data report in both TSV and JSONL formats. (nih.gov)

In addition, five ABA-responsive elements were enriched in CmNAC1 promoter. (frontiersin.org)

Anatomy18

Organisms21

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment30

Psychiatry and Psychology1

Phenomena and Processes79

Disciplines and Occupations1

Information Science3

Proteins11

Genes5

Endo-siRNAs3

MiRNAs2

Protein16

Endogenous1

Post-transcriptional1

Subcellular2

Genome2

LncRNAs1

Assays3

Interference1

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Eukaryotic1

MRNAs1

CircRNAs4

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CDNA1

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Gene4

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Tissues1

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Downstream1

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Factors2

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