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  • methylation
  • The heterochromation formation in yeast is mediated by RNAi-directed silencing mechanism, while the establishment of DNA methylation in plants is through the RNA-directed DNA methylation (RdDM) pathway. (mdpi.com)
  • transcription
  • RNA splicing is a stage in gene transcription . (wikipedia.org)
  • Poly A messenger RNA is then purified from total RNA and subsequently translated into cDNA using a reverse transcription reaction. (wikipedia.org)
  • To maintain the pluripotency of the embryonic stem cells, transcription factors and epigenetic modifiers play an important role despite the fact that little is known about the regulation of pluripotency throughout the process of splicing. (wikipedia.org)
  • 2002
  • The luncheon speaker at Splicing 2002 will be UMBI President Dr. Jennie Hunter-Cevera, an innovative leader in biotechnology with extensive background both in academia and industry. (bio-medicine.org)
  • intronic
  • However, it is noted that the specific sequence of intronic splicing elements and the number of nucleotides between the branchpoint and the nearest 3' acceptor site affect splice site selection. (wikipedia.org)
  • tumor cell
  • In the new study, Beck and research assistant professor Xiaolong He show that knocking down PTB expression with small, interfering RNA "substantially impairs ovarian tumor cell growth, colony formation and invasiveness. (bio-medicine.org)
  • To the best of our knowledge, this is the first time anyone has shown that interference with the production of a splicing factor can inhibit tumor cell growth and tumor invasiveness," Beck said. (bio-medicine.org)
  • Formation
  • The two reactions are known as transesterification reactions because they require the breaking of one strand of RNA and formation of a new ester linkage. (blogspot.com)
  • Recently, splicing factors are reported to be involved in both RNAi-directed heterochromatin formation in yeast and the RdDM pathway in plants. (mdpi.com)
  • In yeast, splicing factors may provide a platform for facilitating the siRNA generation through an interaction with RDRC and thereby affect the heterochromatin formation, whereas in plants, various splicing factors seem to act at different steps in the RdDM pathway. (mdpi.com)
  • post-translationa
  • The first steps of the DDR promote the post-translational modification of splicing factors to affect their localization and activity, while more downstream DDR events alter their expression. (mdpi.com)
  • snRNPs
  • The spliced RNA is released, the lariat is released and degraded, and the snRNPs are recycled. (wikipedia.org)
  • The snRNAs are complimentary to the splice sites and branch sites and that's how the various snRNPs recognize them. (blogspot.com)
  • Cancer
  • A better understanding of how changes in splice site selection are integrated into the DDR may provide new avenues to combat cancer and delay aging. (mdpi.com)
  • For certain types of cancer, like in colorectal and prostate, the number of splicing errors per cancer has been shown to vary greatly between individual cancers, a phenomenon referred to as transcriptome instability. (wikipedia.org)