Loading...
  • genome
  • Small RNAs as guardians of the genome. (nih.gov)
  • In this work we perform the first large scale analysis of the length distribution of untranslated regions (promoters, 5' and 3' untranslated regions, terminators) in the genome of the yeast Saccharomyces cerevisiae . (biomedcentral.com)
  • Epigenetic mechanisms coordinate biological processes such as X-chromosome inactivation, position effect variegation, genomic imprinting, RNA interference, and reprogramming of the genome during differentiation and development leading to gene silencing [ 3 ]. (hindawi.com)
  • Bacterial
  • Currently extensive information exists on the sRNAs of Listeria monocytogenes expressed during growth in extracellular environments.A total of 29 regulatory RNAs, including small non-coding antisense RNAs, are specifically expressed intracellularly.Our analyses reveal extensive sRNA expression as an important feature of bacterial regulation during intracellular growth. (nih.gov)
  • Discovery of new ribonuclease activities in B. subtilis and other Gram-positive species, especially the dual-functioning RNase J1, which specifies both an endonuclease activity and the long-sought bacterial 5'-to-3' exoribonuclease activity, has led to the recognition of intriguing mechanisms of gene regulation at the level of RNA decay. (pubmedcentralcanada.ca)
  • gene expression
  • Untranslated RNAs (utRNAs), also referred to as nonprotein-coding or nonmessenger RNAs ( Brosius and Tiedge, 2004 ), play diverse and important roles in the control of gene expression at the levels of transcription, RNA processing, and translation (for review see Barciszewski and Erdmann, 2003 ). (rupress.org)
  • In this article, we focus on regulated RNA decay in the control of gene expression in Gram-positive organisms, with a focus on Bacillus subtilis . (pubmedcentralcanada.ca)
  • rRNA
  • First, RNA synthesized in vitro competes with the hybridization of [(32)P]rRNA synthesized in vivo to the heavy strand of B. subtilis DNA. (jove.com)
  • Second, unlabeled rRNA synthesized in vivo competes with the hybridization of [(3)H]RNA synthesized in vitro to denatured DNA or heavy-strand DNA, but not to light-strand DNA. (jove.com)
  • Using a mass spectrometry approach, we uncovered single-nucleotide perturbations in dyskerin-guided rRNA modifications, providing functional readouts of small RNA dysfunction in X-DC. (lu.se)
  • eukaryotes
  • Here, we review the conserved roles played by small RNAs in the adaptation of eukaryotes to coexist with their genomic colonists. (nih.gov)
  • molecule
  • After deep-sequencing of the library, adaptor sequences are used to select for the reads covering the full length of the original RNA molecule (both adaptors are observed). (nih.gov)
  • antitoxin
  • Toxin-antitoxin (TA) systems are small genetic elements found in the majority of prokaryotes. (mdpi.com)
  • TA systems controlled by interaction between toxin protein and antitoxin RNA have been identified in S. aureus in silico , but not yet experimentally proven. (mdpi.com)
  • Computational
  • Here, we present a novel automated computational pipeline, named APART, providing a complete workflow for the reliable detection of RNA processing products from next-generation-sequencing data. (nih.gov)
  • functionally
  • The database is functionally expanded from the previously described Tomato Expression Database by including metabolite profiles as well as large-scale tomato small RNA (sRNA) data sets. (nih.gov)
  • Dyskerin associates with hundreds of H/ACA small RNAs to generate a multitude of functionally distinct ribonucleoproteins (RNPs). (lu.se)
  • Thus
  • Thus, BC1 RNA modulates translation-dependent processes in neurons and germs cells by directly interacting with translation initiation factors. (rupress.org)
  • reveal
  • Altogether, these findings reveal that small noncoding RNA dysfunctions may contribute to the pleiotropic manifestation of human disease. (lu.se)
  • cells
  • BC1 RNA, expressed in neurons and germ cells, inhibits a rate-limiting step in the assembly of translation initiation complexes. (rupress.org)
  • We carried out comprehensive profiling of dyskerin-associated H/ACA RNPs, revealing remarkable heterogeneity in the expression and function of subsets of H/ACA small RNAs in X-DC patient cells. (lu.se)
  • data
  • Revealing stable processing products from ribosome-associated small RNAs by deep-sequencing data analysis. (nih.gov)
  • recent
  • However, recent studies suggest that RNA processing can be a multi-layer process leading to the generation of ncRNAs of diverse functions from a single primary transcript. (nih.gov)