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  • differentiation
  • Over-expression of miR-128 reduced Sp1 protein levels and inhibited muscle satellite cell proliferation and differentiation. (springer.com)
  • Inhibition of miR-128 increased Sp1 protein levels and promoted muscle satellite cell differentiation but also suppressed proliferation. (springer.com)
  • siRNAs
  • The S. pombe centromeric repeats are transcribed in both directions, producing double-stranded RNAs (dsRNAs), which are processed into small interfering RNAs (siRNAs) by the endonuclease Dicer. (genetics.org)
  • These include methods and techniques for the isolation and quantitative/qualitative analyses of plant small 21-24 nucleotide RNAs such as small interfering RNAs (siRNAs) and microRNAs (miRNAs) as well as the analysis and manipulation of virus-induced gene silencing (VIGS) in both monocotyledonous and dicotyledenous plants and the use of hairpin RNA (hpRNA) transgenes. (springer.com)
  • Silencing
  • Studies related to pathogen-mediated virus resistance in plants were instrumental in providing some of the historical observations which ultimately led to the vital discovery of double-stranded RNA (dsRNA)-induced gene silencing or RNA interference (RNAi), which has since revolutionized research on plant-virus interactions. (springer.com)
  • detection
  • In RNA Abundance Analysis: Methods and Protocols , expert researchers cover a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. (springer.com)
  • genes
  • Additional analyses of target genes identify miRNAs exhibiting dynamic changes in expression during satellite cell activation. (biomedcentral.com)
  • Mutations in the spn-E and aub genes resulted in the increase of satellite transcript abundance in ovaries and the spn-E 1 mutation causes the accumulation of the histone H3 acetylated at lysine 9 (H3-AcK9) mark and transcription factor TAF1 in satellite chromatin. (genetics.org)
  • RNAi
  • To address whether satellite DNA transcription is under RNAi control, we analyzed the effects of spn-E 1 , spn-E hls-03987 , and aub QC42 mutations on the transcriptional status of the 1.688 satellite DNA. (genetics.org)
  • The intron in centromeric noncoding RNA facilitates RNAi-mediated formation of heterochromatin. (abcam.com)
  • centromeric
  • These results suggest that satellite I RNA is involved in chromosome segregation through controlling activity and centromeric localization of Aurora B kinase. (ovid.com)
  • The strength of the effects of the spn-E mutation differs for 1.688 satellite DNA subfamilies and is more pronounced for autosomal pericentromeric satellites compared to the X-linked centromeric ones. (genetics.org)
  • miRNAs
  • Furthermore, six computationally identified miRNAs demonstrated novel expression through muscle regeneration and in satellite cells. (biomedcentral.com)
  • Three of the six miRNAs were found to regulate satellite cell fate. (biomedcentral.com)
  • Furthermore, miRNAs modulate stem cell fate decisions [ 28 - 31 ] and may have similar functions in satellite cells. (biomedcentral.com)
  • Recent studies identify miR-489 and miR-206 expression in quiescent satellite cells [ 32 , 33 ], however, it is likely that many uncharacterized miRNAs play roles in the transition of a quiescent satellite cell to transit-amplifying myoblast. (biomedcentral.com)
  • helper
  • However, the intense yellow blotch symptoms induced in Nicotiana benthamiana by the YB3 GRV satellite in conjunction with GRV were also manifested when PEMV was the helper. (www.gov.uk)
  • encapsidation
  • Although PEMV RNA-1 was capable of supporting the encapsidation and aphid transmission of the GRV satellite, no evidence was obtained that the essential role of the GRV satellite in the aphid transmission of GRV could be supplied by the PEMV satellite. (www.gov.uk)
  • chromosome
  • We found that knockdown of satellite I RNA causes abnormal chromosome segregation and generation of nuclei with a grape-shape phenotype. (ovid.com)
  • Co-immunoprecipitation experiments showed that satellite I RNA associates with Aurora B, a component of the chromosome passenger complex (CPC) regulating proper attachment of microtubules to kinetochores, in mitotic HeLa cells. (ovid.com)
  • suggesting a role of 1.688 satellites in chromosome congression. (genetics.org)
  • virus
  • In vitro activity of the hairpin ribozyme derived from the negative strand of arabis mosaic virus satellite RNA. (semanticscholar.org)
  • The STMV model, which includes nearly 40 % of the RNA, visible in native electron density maps, has now been refined to 1.4 A resolution, the highest, by a large measure, of any virus. (ucsb.edu)
  • It is the RNA and its distribution within the virion that makes STMV unique, as it provides the best opportunity we have for determining the conformation of the nucleic acid in any spherical virus. (ucsb.edu)
  • symptom
  • The presence of the PEMV satellite in infections with GRV had no effect on symptom expression in Nicotiana spp. (www.gov.uk)