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  • subunit
  • Proteins that interact strongly with the 16S RNA early in subunit assembly stabilize the RNA chain against unfolding in 1 mM Mg2+ and actually promote the formation of a more compact teriary structure in 20 mM Mg2+. (sciencemag.org)
  • The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30 S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. (sciencemag.org)
  • assays
  • Several of the peptides exhibited moderate affinity (in the high nM to low µM range) to modified helix 31 in biophysical assays, including surface plasmon resonance (SPR), and were also shown to bind 30S ribosomal subunits. (nih.gov)
  • C. trachomatis was detected in 19.8% and 6.8% of subjects by the omp1 and 16S rRNA assays, respectively. (lshtm.ac.uk)
  • Proteins
  • A vital function of these proteins may therfore consist in altering the configuration of the RNA so that further assembly reactions can take place. (sciencemag.org)
  • Contacts between helix 31 and the P-site tRNA, initiation factors, and ribosomal proteins highlight the importance of this region in translation. (nih.gov)
  • polymerase
  • Conjunctival samples were tested for the presence of C. trachomatis DNA using a quantitative real-time polymerase chain reaction (PCR) assay for the omp1 gene and for the expression of C. trachomatis 16S rRNA using a 1-step, real-time reverse-transcriptase PCR assay. (lshtm.ac.uk)
  • CONCLUSIONS
  • CONCLUSIONS: The use of 16S rRNA expression for the detection of chlamydial metabolic activity appears to usefully discriminate established infections from the inoculation of the conjunctiva with dead or subviable organisms, which probably occurs frequently in settings in which trachoma is endemic. (lshtm.ac.uk)
  • marker
  • We measured chlamydial 16S ribosomal RNA (rRNA) expression, a marker of chlamydial metabolic activity, in comparison with the quantitation of a chlamydial DNA target in subjects exposed to trachoma. (lshtm.ac.uk)
  • The combination of CRISPR/Cas9 and yeast homologous recombination machinery was achieved by cloning the M. mycoides genome in a strain of yeast expressing Cas9 and then converting it to a non-functional form through the replacement of the "rrs" gene (which encodes for 16S rRNA) with a ura3 yeast marker. (synbiobeta.com)
  • copies
  • For subjects with positive results for both tests, the number of copies of 16S rRNA was approximately 100-fold greater than the number of copies of the omp1 gene. (lshtm.ac.uk)
  • In samples from subjects in whom the omp1 gene was detected in the absence of 16S rRNA, typically only a few copies of omp1 were present. (lshtm.ac.uk)
  • There are 34 predicted RNAs, one predicted copy of 16S rRNA, and four predicted copies of 23S rRNA (Kautharapu and Jarboe 2012). (kenyon.edu)
  • target
  • The cycle starts with exposure of the phage-display peptide library to the target RNA molecules, which are immobilized on a streptavidin surface. (nih.gov)
  • major
  • The antibiotic binds in the major groove of the model A-site RNA within a pocket created by an A-A base pair and a single bulged adenine. (sciencemag.org)