• Binding is dependent on the presence of a highly conserved purine-rich internal loop in the RNA, whereas removal of the loop minimally affects binding of the classical intercalators ethidium bromide and methidiumpropyl-EDTA·Fe (MPE·Fe). (elsevier.com)
  • Aminoglycoside antibiotics that bind to 30 S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. (sciencemag.org)
  • The aminoglycoside antibiotic paromomycin binds specifically to an RNA oligonucleotide that contains the 30 S subunit A site, and the solution structure of the RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. (sciencemag.org)
  • Specific interactions occur between aminoglycoside chemical groups important for antibiotic activity and conserved nucleotides in the RNA. (sciencemag.org)
  • Aminoglycoside-producing Actinobacteria are known to protect themselves from their own aminoglycoside metabolites by producing 16S ribosomal RNA methyltransferase (16S-RMTase), which prevents them from binding to the 16S rRNA targets. (fujita-hu.ac.jp)
  • Although the direct clinical impact of high-level aminoglycoside resistance resulting from production of 16S-RMTase is yet to be determined, ongoing spread of this mechanism will further limit treatment options for multidrug-resistant and extensively drug-resistant gram-negative infections. (fujita-hu.ac.jp)
  • Doi, Y , Wachino, JI & Arakawa, Y 2016, ' Aminoglycoside Resistance: The Emergence of Acquired 16S Ribosomal RNA Methyltransferases ', Infectious Disease Clinics of North America , 巻. (fujita-hu.ac.jp)
  • Following the team's milestone of creating the smallest genome in an independent organism this past March , JCVI/SGI have now published a one-step process for engineering Mycoplasma mycoides 16s rRNA using a combination of CRISPR/Cas9 gene editing technology and yeast recombination machinery. (synbiobeta.com)