Loading...
  • detect
  • mutaFISH™ EGFR T790M T790wt RNA Probes is designed to detect human EGFR T790M gene mutation on single strand RNA in cells using in situ rolling-circle amplication technology. (abnova.com)
  • It is used as a molecular probe to detect DNA or RNA. (wikipedia.org)
  • ISH can also use two or more probes, labeled with radioactivity or the other non-radioactive labels, to simultaneously detect two or more transcripts. (wikipedia.org)
  • Transferred proteins are analyzed for post-translational modifications using probes that may detect lipids, carbohydrate, phosphorylation or any other protein modification. (wikipedia.org)
  • Eastern blotting should be used to refer to methods that detect their targets through specific interaction of the PTM and the probe, distinguishing them from a standard far-western blot. (wikipedia.org)
  • as a technique which probes proteins blotted to PVDF membrane with lectins, cholera toxin and chemical stains to detect glycosylated, lipoylated or phosphorylated proteins. (wikipedia.org)
  • In order to robustly detect and quantify gene expression from small amounts of RNA, amplification of the gene transcript is necessary. (wikipedia.org)
  • rRNA
  • Enhanced sensitivity of DNA- and rRNA-based stable isotope probing by fractionation and quantitative analysis of isopycnic centrifugation gradients. (semanticscholar.org)
  • Not all scaRNAs are composite C/D and H/ACA box snoRNA and most scaRNAs are structurally and functionally indistinguishable from snoRNAs, directing ribosomal RNA (rRNA) modification in the nucleolus. (wikipedia.org)
  • Further investigation showed that scaRNA pugU6-40 targets U6 snRNA, whose modification occurs in the nucleolus not CB and that pugU1-6 and pug U2-55 guide 2 RNAs: snRNA and 28s rRNA. (wikipedia.org)
  • sequence
  • A target sequence and a probe are essential in FISH. (wikipedia.org)
  • The probe hybridizes to the target sequence at elevated temperature, and then the excess probe is washed away (after prior hydrolysis using RNase in the case of unhybridized, excess RNA probe). (wikipedia.org)
  • For miRNA detection, the probes use proprietary chemistry for specific detection of miRNA and cover the entire miRNA sequence. (wikipedia.org)
  • For a typical unbranched, un-crosslinked biopolymer (such as a molecule of a typical intracellular protein, or of DNA or RNA), the primary structure is equivalent to specifying the sequence of its monomeric subunits, such as peptides or nucleotides. (wikipedia.org)
  • Some examples of such motifs are: the C/D and H/ACA boxes of snoRNAs, LSm binding site found in spliceosomal RNAs such as U1, U2, U4, U5, U6, U12 and U3, the Shine-Dalgarno sequence, the Kozak consensus sequence and the RNA polymerase III terminator. (wikipedia.org)
  • The probe thereby hybridizes to single-stranded nucleic acid (DNA or RNA) whose base sequence allows probe-target base pairing due to complementarity between the probe and target. (wikipedia.org)
  • characterization
  • The assay was made possibleby the discovery and characterization of bacteriophageencodedDNA-dependent RNA polymerases (SP6 T7and T3). (bio-medicine.org)
  • The assay was made possible by the discovery and characterization of bacteriophageencoded DNA-dependent RNA polymerases (SP6, T7, and T3). (bio-medicine.org)
  • Holmes CE, Carter BJ, Hecht SM (1993) Characterization of Fe (II)-bleomycin RNA strand scission. (springer.com)
  • Molecular
  • Nucleic acid analogues are compounds which are analogous (structurally similar) to naturally occurring RNA and DNA, used in medicine and in molecular biology research. (wikipedia.org)
  • In molecular biology, a hybridization probe is a fragment of DNA or RNA of variable length (usually 100-1000 bases long) which can be radioactively labeled. (wikipedia.org)
  • In molecular biology, Small Cajal body-specific RNA 1 (also known as SCARNA1 or ACA35) is a small nucleolar RNA found in Cajal bodies and believed to be involved in the pseudouridylation of U2 spliceosomal RNA at residue U89. (wikipedia.org)
  • Microarrays
  • Microarray analysis techniques are used in interpreting the data generated from experiments on DNA, RNA, and protein microarrays, which allow researchers to investigate the expression state of a large number of genes - in many cases, an organism's entire genome - in a single experiment. (wikipedia.org)
  • tRNA
  • Famous examples include the Rho-independent terminator stem loops and the transfer RNA (tRNA) cloverleaf. (wikipedia.org)
  • bind
  • We report that the OH of spectinomycin and the peptide group of vancomycin can bind to the bases of RNA, which might depend on Mg 2+ concentration. (springer.com)
  • cleavage
  • Carter B, de Vroom E, Long EC, Van der Marel GA, Van Boom JH, Hecht SM (1990) Site-specific cleavage of RNA by Fe (II) Bleomycin. (springer.com)
  • vivo
  • Zimmerman JM, Maher LJ III (2002) In vivo selection of spectinomycin-binding RNAs. (springer.com)
  • yeast
  • Bleomycin on the other hand does not show such a drastic effect on yeast total RNA. (springer.com)
  • fluorophore
  • I have some fluorophore labeled RNA probes that I want to try in a Northern Blot analysis using Qiagen RNeasy kit purified RNA. (protocol-online.org)
  • Does any have any "protocol suggestions" for me to use these expensively made custom fluorophore RNA probes? (protocol-online.org)
  • First, the probe is labeled with either direct or indirect labeling strategy: hapten-modified nucleotides are used in indirect labeling, and fluorophore-modified nucleotides are used in direct labeling. (wikipedia.org)
  • microbial
  • RNA-SIP results showed that the composition of enriched active microbial communities were dependent on soil properties, especially the soil pH and grain size. (bioportfolio.com)
  • 1998
  • Djoman MC, Neault JF, Hashemi-Fesharaky S, Tajimir-Riahi HA (1998) RNA-ascorbate interaction. (springer.com)
  • Grüter P et al (1998) TAP, the Human Homolog of Mex67p, mediates CTE-dependent RNA export from the nucleus. (springer.com)