AnatomyOrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
MicroRNAsRNA Polymerase IIDNA-Directed RNA PolymerasesRNARNA Polymerase IIIRNA Polymerase IRNA, Small InterferingRNA, ViralTranscription, GeneticBase SequenceGene Knockdown TechniquesMolecular Sequence DataRNA, RibosomalRNA SplicingRNA EditingRNA InterferenceRNA, BacterialRNA, MessengerTemplates, GeneticPromoter Regions, GeneticPolymerase Chain ReactionDNA-Directed DNA PolymeraseEscherichia coliRNA ReplicaseRNA VirusesTranscription FactorsRNA, Double-StrandedAmino Acid SequenceAmanitinsNucleic Acid ConformationRNA, FungalMutationHeLa CellsSigma FactorRNA, Small NuclearTranscriptional Elongation FactorsProtein BindingRNA Processing, Post-TranscriptionalRNA, CatalyticRNA, TransferDNACell LineRNA FoldingSaccharomyces cerevisiaeRNA PrecursorsRNA StabilityRNA, AntisenseBinding SitesRNA HelicasesPlasmidsDNA Polymerase IRNA CapsKineticsDNA Polymerase IIRNA, UntranslatedTranscription Factors, TFIICell NucleusBacteriophage T7RNA Polymerase Sigma 54Viral ProteinsTerminator Regions, GeneticReverse Transcriptase Polymerase Chain ReactionDNA Polymerase IIIDNA, ViralDNA PrimersRNA-Binding ProteinsRNA, PlantSequence Analysis, RNAMacromolecular SubstancesVirus ReplicationGene Expression RegulationDNA-Binding ProteinsTranscription Factors, GeneralSaccharomyces cerevisiae ProteinsBacterial ProteinsProtein Structure, TertiaryTATA-Box Binding ProteinRNA, ProtozoanPol1 Transcription Initiation Complex ProteinsRNA, NeoplasmModels, MolecularCell Line, TumorT-PhagesDNA, BacterialCloning, MolecularNucleic Acid HybridizationSequence Homology, Amino AcidChromatinOperonProtein BiosynthesisTranscription Factor TFIIBDEAD-box RNA HelicasesNuclear ProteinsDNA Polymerase betaGene SilencingGene Expression Regulation, BacterialProtein SubunitsPotassium PermanganateNucleotidesDNA, Ribosomal
MiRNAsLncRNAsQuantitative polymeraPromoted cell proliferationGenesEndogenousTranscriptionalGene expressionDetermined by quantitative real-timLong noncodinTissuesMechanismsProteinNoncoding RNAsProteinsTranscriptomeReporter assayMoleculesSiRNAsDifferentiationNasopharyngeal carcinomaSiRNAApoptosisGenomeMiRNAInterferenceCircRNAsSingle-strandedTherapeuticNegativelySignificantlyPromotesRNAiRISCProliferationBindsConverselyChain ReactionMetastasisOverexpressionViralMediatorImmunoprecipitationVirusesTumor growthDysregulationPathwayFunctional
MiRNAs22
  • microRNAs (miRNAs) are short (20-24 nt) non-coding RNAs that are involved in post-transcriptional regulation of gene expression in multicellular organisms by affecting both the stability and translation of mRNAs. (cancerindex.org)
  • miRNAs are transcribed by RNA polymerase II as part of capped and polyadenylated primary transcripts (pri-miRNAs) that can be either protein-coding or non-coding. (cancerindex.org)
  • Previously, it has been demonstrated that certain transcriptional targets of SOX4 are associated with cancer development and progression and the processing of microRNAs (miRNAs/miRs) ( 12 ). (spandidos-publications.com)
  • miRNAs are small non-coding RNAs (~22 nucleotides in length) that regulate the expression of their target genes by translational repression or mRNA degradation ( 13 , 14 ). (spandidos-publications.com)
  • Differential expression of miRNAs has been widely described in breast cancers, and suggests that certain miRNAs, including miR-206 ( 19 ), miR-129 ( 20 ), miR-200 ( 21 ) and miR-34 ( 22 ), may function as oncogenes or tumor suppressors in breast cancer. (spandidos-publications.com)
  • To understand the molecular mechanism of SOX4 in breast cancer development and progression, the present study aimed to identify miRNAs that regulate the expression of SOX4, which revealed miR-320 as a potential candidate. (spandidos-publications.com)
  • Emerging evidence indicates that Long non-coding RNAs (LncRNAs) and microRNAs (miRNAs) play crucial roles in tumor progression, including hepatocellular carcinoma (HCC). (biomedcentral.com)
  • A series of studies indicate that lncRNAs serve as competing endogenous RNAs (ceRNA) by sponging miRNAs, and modulate the targets of miRNAs [ 15 , 16 ]. (biomedcentral.com)
  • The aberrant expression of miRNAs is associated with the pathogenesis of sepsis. (degruyter.com)
  • MicroRNAs (miRNAs) are a kind of small noncoding RNAs that regulate the expression of protein through targeting the 3′UTR of messenger RNA (mRNA), resulting in mRNA degradation or suppression of translation [ 5 ]. (degruyter.com)
  • EBV is the first human virus that has the capability to encode microRNAs (miRNAs). (ijbs.com)
  • EBV miRNAs also inhibit the expression of viral antigens, thereby enabling infected cells to escape immune recognition. (ijbs.com)
  • microRNAs (miRNAs) are a large class of small non-coding RNAs with a length of 21-23 nucleotides (nt) [ 11 ]. (ijbs.com)
  • Exceptionally, a minority of miRNAs are derived from RNA Pol III transcripts. (ijbs.com)
  • For example, murine gammaherpesvirus 68 (MHV-68)-encoded miRNAs are located downstream of viral tRNAs and transcribed by RNA Pol III [ 2 ]. (ijbs.com)
  • miRNAs are able to post-transcriptionally control gene expression by binding to target mRNAs [ 15 ]. (ijbs.com)
  • MicroRNAs (miRNAs) are the most altered subset of ncRNAs, followed by long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs). (ersjournals.com)
  • We highlight the role of SE-driven genes, enhancer RNAs (eRNAs), lncRNAs, and miRNAs in the digestive system tumor growth and progression. (ijbs.com)
  • miRNAs comprise a large group of endogenous non-coding RNAs that can block mRNA translation or negatively regulate mRNA stability and thereby play a central role in regulating gene expression [10] , [11] . (plos.org)
  • Small non-protein-coding RNA molecules, composed of around 22 nucleotides, are commonly named as miRNAs ( 1 - 3 ). (frontiersin.org)
  • Indeed, it has been shown that miRNAs play an important role in gene expression, mainly when associated with the monitoring of several cell and metabolic pathways, being also an essential component of the gene silencing machinery in most eukaryotic organisms ( 4 , 8 ). (frontiersin.org)
  • CircRNAs exert their biological effects by 4 different mechanisms: serving as sponges of microRNAs (miRNAs), serving as sponges of RNA binding proteins (RBPs), modulating parental gene transcription, and encoding functional proteins. (techscience.com)
LncRNAs8
  • Pseudogene, a subclass of long non-coding RNAs (lncRNAs), are considered as genomic loci that resemble real gene, but lost some functionality because they are lack of protein-coding ability because of disabling mutation, lack of transcription, or their inability to encode RNA [ 3 ]. (biomedcentral.com)
  • In this review, we focus on the impact of ncRNA post-transcriptional regulatory mechanisms, especially those of microRNAs and lncRNAs, in RA signalling pathways during differentiation and disease. (mdpi.com)
  • The aim of this mini-review is to discuss emerging epigenetic roles of long non-coding RNAs (lncRNAs) in Acute Myeloid Leukemia (AML). (frontiersin.org)
  • In particular, there are incremental examples of the participation of the distinct class of long non-coding RNAs (lncRNAs) in AML epigenetic regulation, some of which have translational potential. (frontiersin.org)
  • Dysfunctions of long non-coding RNA (lncRNAs) have been associated with the initiation and progression of hepatocellular carcinoma (HCC), but the clinicopathologic significance and potential role of lncRNA PTTG3P (pituitary tumor-transforming 3, pseudogene) in HCC remains largely unknown. (biomedcentral.com)
  • We compared the expression profiles of lncRNAs in 3 HCC tumor tissues and adjacent non-tumor tissues by microarrays. (biomedcentral.com)
  • And those greater than 200 nt in length with limited or no protein-coding capacity are generally defined as long non-coding RNAs (lncRNAs [ 6 ]. (biomedcentral.com)
  • Mounting evidence supports that long noncoding RNAs (lncRNAs) have critical roles during cancer initiation and progression. (biomedcentral.com)
Quantitative polymera3
Promoted cell proliferation2
Genes17
  • The initiating dsRNA can also be endogenous (originating in the cell), as in pre-microRNAs expressed from RNA-coding genes in the genome. (wikipedia.org)
  • Our analysis revealed subsets of genes, whose expression is positively associated with H3K27me3, different to the otherwise reported negative association with gene expression in many other organisms. (uni-frankfurt.de)
  • qPCR and Western blot were used to analyze LncRNA PTTG3P, miR-383 and other target genes' expression. (biomedcentral.com)
  • Previous findings indicate that circRNAs mainly function as microRNA (miRNA) sponges to regulate the transcription and posttranscription of miRNA-targeted genes. (hindawi.com)
  • Identification of miRNA-regulated targeting genes is important for understanding their specific biological functions [ 11 ]. (degruyter.com)
  • First identified in embryonic stem cells, SEs mediate pluripotent state maintenance by promoting the expression of pluripotent identity genes, including Oct4 , Sox2 , Nanog and Klf4 . (nature.com)
  • Microarray datasets of HF were downloaded from the Gene Expression Omnibus (GEO) database and differentially expressed genes (DEGs) were selected by using R software. (researchsquare.com)
  • Hub genes were selected by a protein-protein interaction (PPI) network, and competitive endogenous RNA (ceRNA) networks were constructed by Cytoscape. (researchsquare.com)
  • We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
  • In this study, the authors used DNA microarray to compare and identify genes induced by HER2 in mammary epithelial cell line with ectopic HER2 overexpression and breast cancer cell lines derived from patients with different level of HER2 expression. (biomedcentral.com)
  • Four latency types (0, I, II, III) are defined depending on the expression pattern of EBV latent genes, and at the same time are linked to a particular tumor type [ 6 , 7 ]. (ijbs.com)
  • The multistep transformation of normal cells into malignant cells involves genetic and epigenetic alterations that promote the aberrant expression of critical oncogenes and tumor suppressor genes [ 1 , 2 ]. (ijbs.com)
  • Furthermore, we totally predicted 54 target genes by vsiRNAs with psRNATarget server, including disease/stress response-related genes, RNA interference core components, cytoskeleton-related genes, photosynthesis or energy supply related genes. (ppjonline.org)
  • All target genes were down-regulated with different degree by vsiRNAs as shown by real-time reverse transcription polymerase chain reaction. (ppjonline.org)
  • Taken together, CymMV and ORSV siRNAs played important roles in interplay with P. equestris by down modulating the expression levels of endogenous genes in host plant. (ppjonline.org)
  • Mechanistically, over-expression of PTTG3P up-regulated PTTG1, activated PI3K/AKT signaling and its downstream signals including cell cycle progression, cell apoptosis and epithelial-mesenchymal transition (EMT)-associated genes. (biomedcentral.com)
  • Thus, gene expression must be tightly regulated so that only appropriate genes are expressed in a particular cell type. (ernolaszlo.com)
Endogenous2
  • Further, we developed a pipeline to characterise novel genome-wide endogenous short interfering RNAs (endo-siRNAs). (uni-frankfurt.de)
  • Mechanistically, PTTG3P upregulated the expression of miR-383 targets Cyclin D1 (CCND1) and poly ADP-ribose polymerase 2 (PARP2) by sponging miR-383, acting as a competing endogenous RNA (ceRNA). (biomedcentral.com)
Transcriptional4
  • RNA interference (RNAi) is a biological process in which RNA molecules are involved in sequence-specific suppression of gene expression by double-stranded RNA, through translational or transcriptional repression. (wikipedia.org)
  • SNHG16-miR-23-3p/miR-103-5p/HAPLN1 and XIST-miR-23-3p/ miR-103-5p/HAPLN1 might regulate the disease progression of HF at the transcriptional level. (researchsquare.com)
  • Non (protein)-coding RNAs are the most abundant transcriptional products of the coding genome, and comprise several different classes of molecules with unique lengths, conformations and targets. (frontiersin.org)
  • The ppGpp binds to the transcription factor DksA and RNA polymerase to orchestrate extensive transcriptional changes that repress proliferation and promote stress survival (8, 9). (duke.edu)
Gene expression13
  • Principal component analysis (PCA) showed significant differences in gene expression among the four muscles. (bvsalud.org)
  • Further, we developed a Random Forests classifier to predict gene expression using genic (gene length, intron frequency, etc.) and epigenetic features. (uni-frankfurt.de)
  • Upon evaluating different feature sets, we found that genic features are as predictive, of gene expression, as the epigenetic features. (uni-frankfurt.de)
  • We used Shapley local feature explanation values, to suggest that high H3K4me3, high intron frequency, low gene length, high sRNA, and high GC content are the most important elements for determining gene expression status. (uni-frankfurt.de)
  • Search the gene expression profiles from curated DataSets in the Gene Expression Omnibus (GEO) repository. (cancerindex.org)
  • These elements are involved in control of gene expression which governs the phenotype and also plays role in disease biology. (researchgate.net)
  • The term "epigenetics" was initially defined by Conrad Waddington in the 1940s, but in the modern context, it was formally defined in the 1990s by Wolffe and Matzkeset as "the study of heritable changes in gene expression that occur without a change in DNA sequence" (Alokail 2015). (ernolaszlo.com)
  • Epigenetic alterations, acting both independently and together with increasing mutational burden, genomic instability, and stem cell exhaustion, can influence gene expression in ways that promote aging (Saul 2021). (ernolaszlo.com)
  • Recent data show that these extrinsic environmental factors synergize with intrinsic age-related changes to influence epigenetic regulation of gene expression, ultimately contributing to the visible-and invisible-signs of skin aging (Chevalier 2019). (ernolaszlo.com)
  • Subsequent sections focus on the epigenetic regulation of gene expression and its relevance to skin aging. (ernolaszlo.com)
  • However, cell types are differentiated by their program of gene expression. (ernolaszlo.com)
  • In this review we focus on the MYC network (also known as the MAX-MLX Network), a highly conserved super-family of related basic-helix-loop-helix-zipper (bHLHZ) proteins that functions to integrate extracellular and intracellular signals and modulate global gene expression. (engineering.org.cn)
  • Interestingly, hnRNPA1 influenced NP gene expression and affected viral replication. (cdc.gov)
Determined by quantitative real-tim1
Long noncodin1
  • Gao YL, Zhao ZS, Zhang MY, Han LJ, Dong YJ and Xu B: Long noncoding RNA PVT1 facilitates cervical cancer progression via negative regulating of miR-424. (spandidos-publications.com)
Tissues3
  • In this study, we found that PTTG3P was up-regulated in HCC tissues and cells. (biomedcentral.com)
  • Transforming growth factor-β (TGF-β) signaling pathway is a multifunctional cytokine and plays important roles in regulating cell proliferation, cell cycle, differentiation, migration, and apoptosis in a broad spectrum of tissues [ 12 ]. (degruyter.com)
  • Recent research has revealed that PVT1 is up regulated in nasopharyngeal carcinoma tissues and its overexpression predicts a poor prognosis for NPC patients. (biomedcentral.com)
Mechanisms5
  • However, the primary mechanisms regulating osteogenic differentiation are controversial. (nature.com)
  • Aberrant changes in epigenetic mechanisms regulating DNA methylation, histone methylation and acetylation, expression of noncoding RNAs, and mRNA methylation are associated with the initiation, growth, and progression of digestive system tumors [ 2 , 4 ]. (ijbs.com)
  • Mechanisms regulating cardiomyocyte cell cycle arrest are of great interest partly because reversing this process could provide a way to stimulate cardiac regeneration after injury [ 17 ]. (springer.com)
  • Thus, FLI1 drives tumor metastasis not only through the canonical oncoprotein pathway, but also by using epigenetic mechanisms mediated by its exonic circular RNA. (biomedcentral.com)
  • Dual-luciferase reporter assay and RNA pull-down experiments were used to examine the mechanisms interaction between molecules. (biomedcentral.com)
Protein10
  • Scope includes mutations and abnormal protein expression. (cancerindex.org)
  • Exogenous dsRNA initiates RNAi by activating the ribonuclease protein Dicer, which binds and cleaves dsRNAs in plants, or short hairpin RNAs (shRNAs) in humans, to produce double-stranded fragments of 20-25 base pairs with a 2-nucleotide overhang at the 3′ end. (wikipedia.org)
  • Mechanistically, SEs recruited bromodomain containing 4 (BRD4) at the site of ZBTB16 , which then bound to RNA polymerase II-associated protein 2 (RPAP2) that transported RNA polymerase II (POL II) into the nucleus. (nature.com)
  • This kinase, as well as CDK4, has been shown to phosphorylate, and thus regulate the activity of, tumor suppressor protein Rb. (cancerindex.org)
  • They found that HER2 overexpression activated FASN promoter and transcription as well as increased protein production and activity, while inhibitors of HER2, Herceptin and CI-1003, attenuated the effect of HER2 on FASN expression. (biomedcentral.com)
  • PI3K activity was thought to be the mediator of the HER2 control on FASN expression because LY294002, a known PI3K inhibitor, abrogated HER2 induced FASN protein production in the HER2 overexpressing normal mammary epithelial and breast cancer cells. (biomedcentral.com)
  • However, a growing body of evidence suggests that recruitment, stability, and function of epigenetic factors can be mediated by non-protein-coding RNAs. (frontiersin.org)
  • They are usually generated from either exons or introns of both protein-coding and non-coding transcripts that are processed by RNA polymerase II (Pol II) [ 12 - 14 ]. (ijbs.com)
  • qRT-PCR and western blot were performed to detect the gene and protein expression. (biomedcentral.com)
  • Conversely, hnRNPA1 overexpression reduced NP expression at the mRNA and protein levels and impeded virus replication by (60.70%), suggesting antagonistic function. (cdc.gov)
Noncoding RNAs6
  • CircRNAs are newly discovered noncoding RNAs formed through the alternative splicing of premessenger RNA (mRNA). (hindawi.com)
  • In recent years, noncoding RNAs (ncRNAs), especially microRNAs, long noncoding RNAs and circular RNAs, have been found to be associated with abnormalities of the ASMCs. (ersjournals.com)
  • This review highlights findings concerning noncoding RNAs (ncRNAs) in asthmatic ASMCs so far and proposes a schematic diagram that helps us to better understand how ncRNAs are involved in pathophysiological behavioural changes in asthmatic ASMCs. (ersjournals.com)
  • Many noncoding RNAs (ncRNAs) have been identified in asthmatic ASMCs in recent years [ 5 ]. (ersjournals.com)
  • The core transcription regulatory loop of the digestive system tumors is complex and a high density of transcription regulatory complexes in the SEs and the crosstalk between SEs and the noncoding RNAs. (ijbs.com)
  • Circular RNAs (circRNAs) are noncoding RNAs with a covalently closed loop structure. (techscience.com)
Proteins3
  • In the present study, polymerase chain reaction and western blot analyses were performed to examine the expression levels of miR‑130a and associated proteins. (spandidos-publications.com)
  • After transcription, the pre-mRNA transcript is spliced, joining coding exons together while excising introns and generating messenger RNA (mRNA), which is exported from the nucleus and read by ribosomes to produce polypeptides that fold into the final 3-dimensional structure of proteins ( Figure 1.1) . (ernolaszlo.com)
  • Abstract Scaffold proteins are crucial regulators of signaling networks, and their abnormal expression may favor the development of tumors. (techscience.com)
Transcriptome1
  • We find that the small RNA transcriptome is altered, even if a gene unrelated to RNAi pathway is targeted. (uni-frankfurt.de)
Reporter assay2
  • Luciferase reporter assay and RNA pull-down assay were carried out to detect the interaction between miR-383 and LncRNA PTTG3P. (biomedcentral.com)
  • The binding sites of miR-128-3p and TGFBR2 were predicted by Targetscan online software and confirmed by dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay. (degruyter.com)
Molecules4
  • Two types of small ribonucleic acid (RNA) molecules, microRNA (miRNA) and small interfering RNA (siRNA), are central to components to the RNAi pathway. (wikipedia.org)
  • It is initiated by the enzyme Dicer, which cleaves long double-stranded RNA (dsRNA) molecules into short double-stranded fragments of approximately 21 to 23 nucleotide siRNAs. (wikipedia.org)
  • RNAi is an RNA-dependent gene silencing process that is controlled by RISC and is initiated by short double-stranded RNA molecules in a cell's cytoplasm, where they interact with the catalytic RISC component Argonaute. (wikipedia.org)
  • A growing body of evidence indicates that epigenetic regulators rely, amongst other factors, on their interaction with untranslated RNA molecules for guidance to particular targets on DNA. (frontiersin.org)
SiRNAs4
  • We also predicted phasing of siRNAs, which are regulated by the RNA interference (RNAi) pathway. (uni-frankfurt.de)
  • Further, using RAPID, we investigated the aberrations of endo-siRNAs, and their respective transcriptomic alterations caused by an RNAi pathway triggered by feeding small RNAs against a target gene. (uni-frankfurt.de)
  • CymMV and ORSV small interfering RNAs (siRNAs) demonstrated several general and specific/new characteristics. (ppjonline.org)
  • In eukaryotes, upon viral infection RNAi employs Dicer and Dicer-like (DCL) enzymes to cleave viral RNAs into small interfering RNAs (siRNAs) with sizes about 21 nucleotides (nt), which are further amplified by RNAdependent RNA polymerases (RdRPs). (ppjonline.org)
Differentiation1
  • In patients with breast cancer, expression of FLI1 is strongly correlated with advanced stage, poor differentiation, and lymph node metastasis. (biomedcentral.com)
Nasopharyngeal carcinoma1
  • Zhu K, He Y, Xia C, Yan J, Hou J, Kong D, Yang Y and Zheng G: MicroRNA-15a inhibits proliferation and induces apoptosis in CNE1 nasopharyngeal carcinoma cells. (spandidos-publications.com)
SiRNA6
  • Each siRNA is unwound into two single-stranded RNAs (ssRNAs), the passenger (sense) strand and the guide (antisense) strand. (wikipedia.org)
  • R2D2 carries tandem double-stranded RNA-binding domains to recognize the thermodynamically stable terminus of siRNA duplexes, whereas Dicer-2 the other less stable extremity. (wikipedia.org)
  • We developed a generic automated eukaryotic short interfering RNA (siRNA) analysis tool, called RAPID. (uni-frankfurt.de)
  • Our tool captures diverse siRNA characteristics from small RNA sequencing data and provides easily navigable visualisations. (uni-frankfurt.de)
  • We also introduced a normalisation technique to facilitate comparison of multiple siRNA-based gene knockdown studies. (uni-frankfurt.de)
  • In vitro , the sprouting and migratory properties of primary endothelial cell cultures were reduced by inhibiting EZH2 through up-regulation of miR-101, siRNA-mediated knockdown of EZH2, or treatment with 3-Deazaneplanocin-A (DZNep), a small molecule inhibitor of EZH2 methyltransferase activity. (plos.org)
Apoptosis4
  • Yang M, Zhai X, Ge T, Yang C and Lou G: MiR-181a-5p promotes proliferation and invasion, and inhibits apoptosis of cervical cancer cells via regulating inositol polyphosphate-5-phosphatase A (INPP5A). (spandidos-publications.com)
  • Functional experiments demonstrated that knockdown of PTTG3P inhibited cell proliferation, migration and invasion, and promoted cell apoptosis, acting as an oncogene. (biomedcentral.com)
  • Overexpression of miR-128-3p or knockdown of TGFBR2 ameliorated LPS-induced inflammation and apoptosis. (degruyter.com)
  • Loss-of-function experiments support that PVT1 regulates cell apoptosis by influencing the DNA damage repair pathway after radiation, suggesting that targeting PVT1 may be a potential strategy for NPC therapy [ 11 ]. (biomedcentral.com)
Genome1
  • When the dsRNA is exogenous (coming from infection by a virus with an RNA genome or laboratory manipulations), the RNA is imported directly into the cytoplasm and cleaved to short fragments by Dicer. (wikipedia.org)
MiRNA3
  • The mature miRNA is incorporated into a RNA-induced silencing complex (RISC), which recognizes target mRNAs through imperfect base pairing with the miRNA and most commonly results in translational inhibition or destabilization of the target mRNA. (cancerindex.org)
  • MiRNA usually binds directly to the 3′-untranslated region (3′-UTR) of target gene mRNA through the RNA-induced silencing complex (RISC) [ 15 , 16 ]. (ersjournals.com)
  • We previously showed that VEGF signaling in primary endothelial cell cultures caused overexpression of VEGFR2 in a positive feed-forward loop, which is at least partly regulated by loss of miRNA-mediated control of VEGF receptor degradation. (plos.org)
Interference1
CircRNAs3
  • Exosomes secreted by the umbilical cord mesenchymal stromal cells (UMSCs) and circular RNAs (circRNAs) derived from exosomes have been shown to be associated with the progression of GDM-related complications. (hindawi.com)
  • Studies report that circular RNAs (circRNAs) derived from exosomes may play a key role in GDM development. (hindawi.com)
  • High-throughput RNA sequencing and bioinformatics analysis have led to the identification of several novel circRNAs that exhibit biological characteristics and regulatory functions [ 6 , 9 ]. (hindawi.com)
Single-stranded1
  • When a signal is sent to express a specific gene, the DNA sequence encoding that gene is used as a template to produce single-stranded RNA in a process called transcription. (ernolaszlo.com)
Therapeutic2
  • Antisense RNA produced intracellularly by an expression vector may be developed and find utility as novel therapeutic agents. (wikipedia.org)
  • Therefore, our study shows that SEs orchestrate the osteogenesis of MSCs by targeting ZBTB16 expression, which provides an attractive focus and therapeutic target for osteoporosis. (nature.com)
Negatively3
  • The expression of TIMP2 was negatively mediated by miR‑130a, and HPV18 E6 inhibited the expression of TIMP2 in HeLa cells. (spandidos-publications.com)
  • These results suggest that paraspeckles play a role in trapping nuclear PXR in the absence of the ligand to negatively regulate transactivation of its downstream gene. (aspetjournals.org)
  • Collectively, this is the first study to demonstrate that the paraspeckle components NEAT1_2 and DAZAP1 negatively regulate CYP3A4 induction by PXR. (aspetjournals.org)
Significantly5
  • High expression of miR‑130a was significantly associated with lymph node metastasis and an advanced clinical stage of cervical cancer. (spandidos-publications.com)
  • Knockdown of HPV18 E6 significantly inhibited the expression of miR‑130a in HeLa cervical cancer cells. (spandidos-publications.com)
  • Moreover, individuals supplemented with micro-doses of rhGH exhibited significantly changed urinary steroidal profiles, suggesting a role of rhGH in both energy metabolism and steroidogenesis. (bvsalud.org)
  • Weighted gene co-expression network analysis (WGCNA) identified six modules, which were significantly associated with muscle fiber diameter. (bvsalud.org)
  • Induction of CYP3A4 by rifampicin was significantly enhanced by the knockdown of NEAT1_2 or DAZAP1 in ShP51 cells and their parental HepG2 cells. (aspetjournals.org)
Promotes3
  • In summary, the present study suggests that HPV18 E6 promotes the expression of miR‑130a, which further inhibits the expression of TIMP2 and promotes cervical cancer cell invasion. (spandidos-publications.com)
  • Zhang JJ, Wang DD, Du CX and Wang Y: Long noncoding RNA ANRIL promotes cervical cancer development by acting as a sponge of miR-186. (spandidos-publications.com)
  • Our findings suggest that PTTG3P, a valuable marker of HCC prognosis, promotes tumor growth and metastasis via up-regulating PTTG1 and activating PI3K/AKT signaling in HCC and might represent a potential target for gene-based therapy. (biomedcentral.com)
RNAi1
  • Caco-2 cells were transfected with either the FASN specific RNAi plasmid or the negative control RNAi plasmid, followed by the RT-qPCR and western blot to examine the expression of FASN, HER2, PI3K and Akt. (biomedcentral.com)
RISC1
  • The passenger strand is degraded and the guide strand is incorporated into the RNA-induced silencing complex (RISC). (wikipedia.org)
Proliferation3
  • Zhou Y, Yang C, Wang K, Liu X and Liu Q: MicroRNA-33b inhibits the proliferation and migration of osteosarcoma cells via targeting hypoxia-inducible factor-1α. (spandidos-publications.com)
  • Therefore, MESH1 knockdown leads to both stress survival and proliferation arrest, two cardinal features highly reminiscent of the bacterial stringent response. (duke.edu)
  • RFC4 knockdown induced G2/M cell cycle arrest and inhibited NPC cell proliferation in vitro and in vivo . (engineering.org.cn)
Binds1
  • Using this CasIP assay, we identified FECR1, a FLI1 exonic circular RNA that binds to the FLI1 promoter and epigenetically activates FLI1 in breast cancer cells. (biomedcentral.com)
Conversely1
Chain Reaction2
Metastasis1
  • Artificial modulation of PTTG3P (down- and over-expression) was performed to explore the role of PTTG3P in tumor growth and metastasis in vitro and in vivo. (biomedcentral.com)
Overexpression2
  • Dysregulation of SE-mediated ZBTB16 expression leads to osteoporosis, and bone-targeting ZBTB16 overexpression is efficient in accelerating bone repair and treating osteoporosis. (nature.com)
  • Using a CRISPR Cas9-guided immunoprecipitation assay, we identify a circular RNA in the FLI1 promoter chromatin complex, consisting of FLI1 exons 4-2-3, referred to as FECR1.Overexpression of FECR1 enhances invasiveness of MDA-MB231 breast cancer cells. (biomedcentral.com)
Viral3
  • In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. (ppjonline.org)
  • vsiRNAs, with A/U bias at the first nucleotide, were predominantly 21-nt long and they were derived predominantly (90%) from viral positive-strand RNA. (ppjonline.org)
  • Most importantly, hnRNPA1 knockdown caused a significant increase in NP expression and enhanced viral replication (93.82%) in IAV infected A549 cells. (cdc.gov)
Mediator1
  • SEs are defined as large clusters of active enhancers in large clusters that are enriched with high levels of transcription factors (TFs), master co-factors, mediator complexes, RNA polymerase II (RNA pol II), and epigenetic modifications [ 5 , 7 ]. (ijbs.com)
Immunoprecipitation1
  • Immunoprecipitation assays using PXR-overexpressing HepG2 (ShP51) cells revealed that PXR interacts with not only DAZAP1 but also NEAT1_2, a long noncoding RNA included in the paraspeckle, and that the interaction between PXR and NEAT1_2 was disassociated by rifampicin. (aspetjournals.org)
Viruses1
  • Continuous but heterogeneous distribution and secondary structures prediction implied that vsiRNAs originate predominantly by direct Dicer-like enzymes cleavage of imperfect duplexes in the most folded regions of the positive strand of both viruses RNA molecular. (ppjonline.org)
Tumor growth1
  • These data suggest that FECR1 circular RNA acts as an upstream regulator to control breast cancer tumor growth by coordinating the regulation of DNA methylating and demethylating enzymes. (biomedcentral.com)
Dysregulation1
Pathway1
  • A well-studied major pathway that is possibly involved in regulating FASN expression is the PI3K/Akt pathway. (biomedcentral.com)
Functional1
  • Caco-2 cells with a high expression of both HER2 and FASN were selected for functional characterization. (biomedcentral.com)