Rna polymerase iii. Medical search. FAQ

Abstract
Abstract:'RNA chain elongation through assembled transcription complexes was uneven but relatively rapid: at 20°C with 1 mM NTPs, the fastest RNA chains elongated at an average rate of 29 nucleotides (nt)/second, and the median RNA chains elongated at 21 to 22 nt/second on average. (harvard.edu)

subunits
POLR3A and POLR3C encode subunits of RNA polymerase III. (jci.org)
D ) Schematic representation of POL III highlighting the subunits in the different subcomplexes. (jci.org)
Characterization of human RNA polymerase III identifies orthologues for Saccharomyces cerevisiae RNA polymerase III subunits. (sigmaaldrich.com)
Orthologues of the yeast RNA polymerase III subunits C128 and C37 remain unidentified, and for many of the other subunits, the available information is limited to database sequences with various degrees of similarity to the yeast subunits. (sigmaaldrich.com)
We found that two RNA polymerase III subunits, referred to as RPC8 and RPC9, displayed sequence similarity to the RNA polymerase II RPB7 and RPB4 subunits, respectively. (sigmaaldrich.com)
RPC8 and RPC9 associated with each other, paralleling the association of the RNA polymerase II subunits, and were thus paralogues of RPB7 and RPB4. (sigmaaldrich.com)
RPC5 associated with RPC53, the human orthologue of S. cerevisiae C53, paralleling the association of the S. cerevisiae C37 and C53 subunits, and was required for transcription from the type 2 VAI and type 3 human U6 promoters. (sigmaaldrich.com)
May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. (nih.gov)
Mutations in genes encoding subunits of RNA polymerases I and III cause Treacher Collins syndrome. (nih.gov)
The AC40 and AC19 subunits (encoded by RPC40 and RPC19) are shared by yeast RNA polymerases I and III and have a local sequence similarity to prokaryotic alpha subunits. (pnas.org)

Saccharomyces
This analysis led to the identification of small molecule inhibitors of RNA polymerase (Pol) III from Saccharomyces cerevisiae . (asm.org)
Analysis of RNA chain elongation and termination by Saccharomyces cerevisiae RNA polymerase III. (harvard.edu)
Unlike Saccharomyces cerevisiae RNA polymerase III, human RNA polymerase III has not been entirely characterized. (sigmaaldrich.com)

Epstein-Barr virus-encoded
The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway (By similarity). (nih.gov)
During all typical forms of latent EBV infection and B-cell immortalisation, two highly expressed gene products are the non-polyadenylated RNAs, Epstein-Barr Virus encoded RNAs 1 and 2 (EBER1 and EBER2). (biomedcentral.com)

yeast
Yeast RNA polymerase III transcription factors and effectors. (yeastgenome.org)
U6 snRNA is conserved from yeast to mammals and is transcribed by RNA Polymerase III. (ens-lyon.fr)
Three lines of evidence show that UK-118005 inhibits cell growth by targeting RNA Pol III in yeast. (asm.org)
The yeast Pol III in vitro system has a relatively high rate of chain elongation: at 20°C, with 1 mM NTPs, the fastest chains elongate at 29 nt/seconds and the median elongation rate is 21 to 22 nt/seconds. (harvard.edu)
We present the first comprehensive analysis of RNA polymerase III (Pol III) transcribed genes in ten yeast genomes. (nih.gov)
The data indicate that Rpc11p limits RNA 3′-U length and that this significantly restricts pre-tRNAs to a La-independent pathway of maturation in fission yeast. (pubmedcentralcanada.ca)

PMID
This entry represents a component of the RNA polymerase III (Pol III) complex , which catalyse the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [ PMID: 19631370 ]. (ebi.ac.uk)

chain elongation
An average RNA chain elongation rate of 21 to 22 nt/second at 1 mM NTPs could be derived for the median RNA chain from Figure 2(d), as already mentioned, and an extrapolated value of 7.2 nt/second at 100µM NTPs from Figure 2(c). (harvard.edu)
Investigators] have found that RNA chain elongation by Pol III is also uneven. (harvard.edu)
Overall productive RNA chain elongation proceeds quite rapidly. (reactome.org)

molecules
The identification of these inhibitors demonstrates that RNA Pol III can be targeted by small synthetic molecules. (asm.org)
The RNA polymerase III (pol III) type III promoters U6 and 7SK are routinely used to express short hairpin RNA (shRNA) molecules from a DNA construct. (alliedacademies.org)
Epstein-Barr Virus (EBV)-encoded RNAs (EBERs) are non-polyadenylated RNA molecules transcribed from the EBV genome by RNA polymerase III (pol III). (biomedcentral.com)

ribosomal
The protein encoded by this gene is a component of the RNA polymerase I and RNA polymerase III complexes, which function in the synthesis of ribosomal RNA precursors and small RNAs, respectively. (nih.gov)

gene
Recently, BRF2, a component of TFIIIB required for gene external RNA pol III transcription, was identified as an oncogene in squamous cell carcinomas of the lung through integrative genomic analysis. (biomedcentral.com)

snRNA
The porcine 7SK small nuclear RNA (snRNA) was identified by alignment with known sequences and further characterisation of the upstream regions determined the presence of typical RNA pol III sequence motifs. (alliedacademies.org)
RNA polymerase (pol) III transcription is responsible for the transcription of small, untranslated RNAs involved in fundamental metabolic processes such mRNA processing (U6 snRNA) and translation (tRNAs). (biomedcentral.com)

mutations
E ) Cryo-EM structure of POL III with the identified mutations highlighted in red. (jci.org)

repress
We show that p107 and p130 can both repress Pol III transcription in transient transfection assays or when added to cell extracts. (hud.ac.uk)

deregulation
RNA pol III transcription contributes to the regulation of the biosynthetic capacity of a cell and a direct link exists between cancer cell proliferation and deregulation of RNA pol III transcription. (biomedcentral.com)
Thus, deregulation of RNA pol III transcription can lead to aberrant production of critical RNAs contributing to uncontrolled cell growth, a hallmark trait of many types of cancer. (biomedcentral.com)

transcription factors
Regulation of pol III output is mediated via a number of different mechanisms that can alter the activities of the transcription factors which are responsible for directing pol III transcription. (gla.ac.uk)
Previously EBV has been demonstrated to induce transcription factors associated with EBER expression, including pol III transcription factors and ATF-2. (biomedcentral.com)

protein
Work presented in this thesis aimed at investigating the mechanisms behind the regulation of pol III transcription by the protein kinase CK2. (gla.ac.uk)
Termination by RNA polymerase III (Pol III) produces RNAs whose 3′ oligo(U) termini are bound by La protein, a chaperone that protects RNAs from 3′ exonucleases and promotes their maturation. (pubmedcentralcanada.ca)
RNA polymerase III (Pol III) transcription is subject to repression by the retinoblastoma protein RB, both in vitro and in vivo (R. J. White, D. Trouche, K. Martin, S. P. Jackson, and T. Kouzarides, Nature 382:88-90, 1996). (hud.ac.uk)

promoters contain
The pol III type III promoters contain sequence motifs located within regions referred to as the enhancer and core region (Schramm and Hernandez, 2002). (alliedacademies.org)

regulation of Pol III transcription
This regulation of Pol III transcription is important for energetic economy as mice lacking Maf1 are lean and resist weight gain on normal and high fat diets. (pnas.org)

vivo
Acetylation of the core histone N termini is a post-translational modification of chromatin that has been widely correlated with enhanced transcriptional activity in vivo ( 3 , 34 , 55 , 57 ). (asm.org)
Disruption of this interaction in vivo by using the E7 oncoprotein of human papillomavirus results in a marked increase in Pol III transcription. (hud.ac.uk)

histone
SINE transcription by RNA polymerase III is suppressed by histone methylation but not by DNA methylation. (sigmaaldrich.com)
In contrast, a histone methyltransferase inhibitor selectively promotes SINE expression and occupancy by RNA polymerase III. (sigmaaldrich.com)

synthesis
The origin of this energy demand is proposed to reside in a futile cycle of RNA synthesis and turnover that is enhanced by constitutive Pol III transcription. (pnas.org)
Metabolite profiling also confirms key predictions of the futile RNA cycle hypothesis by identifying changes in many metabolites involved in nucleotide synthesis and turnover. (pnas.org)

construct
This protocol provides a strategy to construct hybrid RNA polymerase III (Pol III) promoters that facilitate high expression of sgRNA and improved CRISPR-Cas9 function. (bio-protocol.org)

respectively
The 'A or G' sparing rule ('ΔG' or 'ΔA') as well as 'ΔU' and 'ΔC' rules are summarized at right with 'E', 'A' and 'B' to indicate whether the rule applies to each of the three domains of life (Eukaryotes, Archaea and Bacteria, respectively). (nih.gov)

assay
By testing analogs of UK-118005 in a template-specific RNA Pol III transcription assay, an inhibitor with significantly higher potency, ML-60218, was identified. (asm.org)

fibroblasts
Pol III activity is also deregulated in fibroblasts derived from p107 p130 double knockout mice. (hud.ac.uk)

Function
Here, using targeted metabolomics, we find changes in the liver of fed and fasted Maf1 KO mice consistent with the function of mammalian Maf1 as a chronic Pol III repressor. (pnas.org)
The study cohort included 279 patients with SSc from the observational Oslo University Hospital cohort with complete data on (1) SSc-related autoantibodies, (2) paired, serial analyses of lung function and fibrosis by computed tomography, and (3) PH verified by right heart catheterization. (jrheum.org)

MAF1
Thus, constitutively high levels of Pol III transcription in Maf1 KO mice reprogram central metabolic pathways and waste metabolic energy through a futile RNA cycle. (pnas.org)
Transcription by pol III is tightly regulated in response to growth stage, growth conditions and stress by a central regulator Maf1. (ens-lyon.fr)
Maf1, a new player in the regulation of human RNA polymerase III transcription. (nih.gov)

human
Further examination showed that both compounds are broad-spectrum inhibitors, displaying activity against RNA Pol III transcription systems derived from Candida albicans and human cells. (asm.org)

chromatin
Our results suggest that at least some of the mechanisms are common for both pol III and pol II to tackle the chromatin barriers for transcription. (ens-lyon.fr)

induction
RIG-I-dependent sensing of poly(dA:dT) through the induction of an RNA polymerase III-transcribed RNA intermediate. (nih.gov)

sequences
Bacteria use these stored spacer sequences as a template to express RNA to recognize and attack specific viruses if they are exposed again. (bio-protocol.org)

proliferation
Therefore, the rate of pol III transcription plays a fundamental role in cellular growth and proliferation. (gla.ac.uk)

commonly
2017). More commonly for CRISPR systems, RNA polymerase III (Pol III) promoters have been used to drive sgRNA expression. (bio-protocol.org)

recognize
When combined with CRISPR-associated (Cas) proteins, CRISPR-Cas systems can recognize and cut foreign DNA or RNA, destroying the virus and protecting the host from repeated infections (Barrangou, 2013). (bio-protocol.org)

interactions
May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. (nih.gov)

complex
We have purified an RNA polymerase III complex and identified its components. (sigmaaldrich.com)