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  • interacts
  • The BRCA1 protein associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. (wikipedia.org)
  • It interacts with a homologous region in BARD1 also consisting of a RING finger flanked by two alpha-helices formed from residues 36-48 and 101-116. (wikipedia.org)
  • mammalian
  • We directly tested whether Pol II and Mediator interact in a manner consistent with condensate formation ( 11 - 13 ), quantitatively characterized their biophysical properties, and considered the implications of these observations for transcription regulation in living mammalian cells. (sciencemag.org)
  • Estimates of the average number of unique messenger RNAs that are targets for repression by a typical miRNA vary, depending on the estimation method, but multiple approaches show that mammalian miRNAs can have many unique targets. (wikipedia.org)
  • Mediator
  • We used live-cell superresolution and light-sheet imaging to study the organization and dynamics of the Mediator coactivator and RNA polymerase II (Pol II) directly. (sciencemag.org)
  • Mediator and Pol II each form small transient and large stable clusters in living embryonic stem cells. (sciencemag.org)
  • Mediator and Pol II are colocalized in the stable clusters, which associate with chromatin, have properties of phase-separated condensates, and are sensitive to transcriptional inhibitors. (sciencemag.org)
  • We suggest that large clusters of Mediator, recruited by transcription factors at large or clustered enhancer elements, interact with large Pol II clusters in transcriptional condensates in vivo. (sciencemag.org)
  • Using superresolution ( 7 - 9 ) and light-sheet imaging ( 10 ), we studied the organization and dynamics of endogenous Mediator and Pol II in live mouse embryonic stem cells (mESCs). (sciencemag.org)
  • To visualize Mediator and Pol II in live cells, we generated mESC lines with endogenous Mediator and Pol II labeled with Dendra2, a green-to-red photoconvertible fluorescent protein (materials and methods and figs. S1 and S2). (sciencemag.org)
  • Mediator exists in a population of transient small (~100 nm) clusters ( Fig. 1B ) with an average lifetime of 11.1 ± 0.9 s (mean ± SEM from 36 cells) ( Fig. 1G ), comparable to that of transient Pol II clusters observed in this study ( Fig. 1, D, E, and H ) and previously in differentiated cell types ( 14 , 15 ). (sciencemag.org)
  • RNAPII
  • The mechanism by which RNA polymerase II (RNAPII) transcribes the nucleosomal DNA remains unclear. (sciencemag.org)
  • Here we report the cryo-electron microscopy structures of RNAPII-nucleosome complexes in which RNAPII pauses at the superhelical locations SHL(−6), SHL(−5), SHL(−2), and SHL(−1) of the nucleosome. (sciencemag.org)
  • transcripts
  • miRNAs resemble the small interfering RNAs (siRNAs) of the RNA interference (RNAi) pathway, except miRNAs derive from regions of RNA transcripts that fold back on themselves to form short hairpins, whereas siRNAs derive from longer regions of double-stranded RNA. (wikipedia.org)
  • RNAP II
  • The enzymes were named according to the order of the elutions, RNAP I, RNAP II, RNAP IΙI. (wikipedia.org)
  • transition
  • In 2000, a second small RNA was characterized: let-7 RNA, which represses lin-41 to promote a later developmental transition in C. elegans. (wikipedia.org)
  • serine
  • It has a kinase activity that phosphorylates the C-terminal domain (CTD) of Pol II at the amino acid serine. (wikipedia.org)
  • the Znf C3HC4- RING domain, the BRCA1 serine domain and two BRCT domains. (wikipedia.org)