Rna or protein sequences. Medical search. Frequent questions

Predicted to be involved in regulation of transcription by RNA polymerase II. (nih.gov)
Multiple-Variable Semi-Covariance Co-Efficiency Analysis of RNA-dependent RNA polymerase Proteins from SARS-CoV-2 variant and an. (mathworks.com)
87(5): 1663-1667] Specifically, the investigators started by priming whole cerebellar RNA with a synthetic oligonucleotide containing a T7 RNA polymerase promoter sequence. (genomicglossaries.com)
After second- strand cDNA synthesis, T7 RNA polymerase was used to generate aRNA. (genomicglossaries.com)
ABSTRACT: Nucleic acid sequence based amplification (NASBA) is an isothermal nucleic acid amplification procedure based on target-specific primers and probes, and the co-ordinated activity of 3 enzymes: AMV reverse transcriptase, RNase H, and T7 RNA polymerase. (int-res.com)
Open-reading frames corresponding to the predicted polymerase protein (polymerase 1a, 1b), spike protein (S), small membrane protein (E), membrane protein (M), and nucleocapsid protein (N), plus several other open-reading frames of unknown function, have been identified. (cdc.gov)

Elucidation of the bovine mammary gland transcriptome by RNA-seq is essential for identifying candidate genes that contribute to milk composition traits in dairy cattle. (biomedcentral.com)
Integrated analysis of differential gene expression, previously reported quantitative trait loci, and genome-wide association studies indicated that TRIB3 , SAA ( SAA1 , SAA3 , and M-SAA3.2 ), VEGFA, PTHLH , and RPL23A were the most promising candidate genes affecting milk protein and fat percentage. (biomedcentral.com)
One basic problem is how a decrease in tRNA levels, by downregulating Pol III efficiency, influences the expression pattern of protein-coding genes. (mdpi.com)
More than half of all human genes are alternatively spliced, so it is expected that alternative pre- mRNA splicing will be responsible for the identification of more than 90 percent of all human proteins. (genomicglossaries.com)
Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes. (genomicglossaries.com)
How do genes direct the production of proteins? (medlineplus.gov)
Most genes contain the information needed to make functional molecules called proteins. (medlineplus.gov)
A few genes produce regulatory molecules that help the cell assemble proteins. (medlineplus.gov)
Through the processes of transcription and translation, information from genes is used to make proteins. (medlineplus.gov)
Evaluation of phenotypic markers and expression of matrisome genes and proteins were evaluated by RNA-sequencing, mass spectrometry and immunohistochemistry. (lu.se)
The program collects whole-genome sequence and other -omics data (In biology, -omics refers to measurable differences or changes in biological molecules, such as genes, metabolites, proteins, and RNA. (cdc.gov)

In summary, we demonstrated a simple strategy to significantly improve the performance for shotgun mass spectrometry by protein-level integrating multiple search engines, maximizing the utilization of the current MS spectra without additional experimental work. (nih.gov)
After 4 weeks of conversion, we performed global analyses of RNA and protein levels by RNA-sequencing and mass spectrometry. (lu.se)

When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
Each protein is also attached to half of a green fluorescent protein (GFP) molecule. (genengnews.com)
These RNA-binding proteins could also be used to build molecular assembly lines that would bring together enzymes needed to perform a series of reactions that produce a drug or another molecule of interest. (genengnews.com)
RNA-like oligonucleotides that are complementary to a portion of a target mRNA molecule. (genomicglossaries.com)
A complementary RNA sequence that binds to a naturally occurring (sense) mRNA molecule, thus blocking its translation. (genomicglossaries.com)
A synthetic, specially- designed oligonucleotide with the ability to recognize and bind a protein ligand molecule or molecules with high affinity and specificity. (genomicglossaries.com)
This proposed new method (FRET X) will allow for sequencing at the single-molecule level. (tudelft.nl)
They synthesized fragments of acyclic (non-circular) L-threoninol nucleic acid (L-aTNA), a molecule that is thought to have existed before RNA came to be. (astrobiology.com)
the process by which a messenger RNA molecule specifies the linear sequence of amino acids on a ribosome for protein synthesis. (dictionary.com)
During the process of transcription, the information stored in a gene's DNA is passed to a similar molecule called RNA (ribonucleic acid) in the cell nucleus. (medlineplus.gov)

Wang and his coworkers 12 investigated the discriminative power of three sequence features from protein sequence, including the side chain pKa value, the hydrophobicity index and the molecular mass of an amino acid. (nature.com)
The protein is 194 amino acids long and has a calculated molecular weight of 21kDa. (wikipedia.org)
report the molecular cloning of Painting of fourth (Pof), which encodes a protein that specifically paints chromosome 4 (Proc Natl Acad Sci USA 2001, 98:6273-6278). (the-scientist.com)
Now a large and ever increasing number of genetic subtypes has been described, and major advances in molecular and cellular biology have clarified the understanding of the role of different proteins in the physiology of peripheral nerve conduction in health and in disease. (medscape.com)
Or it can be built from molecular information, like genetic sequences. (thetech.org)
Aptamers are short nucleic acid sequences capable of specific, high-affinity molecular binding. (genomicglossaries.com)
The flow of information from DNA to RNA to proteins is one of the fundamental principles of molecular biology. (medlineplus.gov)
Molecular mechanisms for regulation of gene expression at different levels: remodeling of chromatin, initiation of transcription, nuclear transport and signalling, and RNA interference. (lu.se)

Together these proteins shorten the end of the mRNAs, preparing the molecules for degradation. (elifesciences.org)
The RNA hairpin is a fundamental structural unit in many RNA molecules and is composed of stems and loops. (genomeweb.com)
But proteins are not boring, they are the most fantastically, well, protean molecules on Earth and possibly anywhere else! (physicsforums.com)
When both proteins find their target sequence, the GFP molecules join and become fluorescent-a signal to the researchers that the target RNA is present. (genengnews.com)
Because alternative RNA splicing allows different types of mRNA molecules to be created from a single gene, it generates the diversity of protein function and structure that is essential to complex organisms. (genomicglossaries.com)
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. (genomicglossaries.com)
More specifically, antisense oligonucleotides that are useful as reagents for target validation , or as drugs, are engineered molecules that differ from natural RNA but that have a base sequence that is recognized as being complementary to a very specific mRNA sequence. (genomicglossaries.com)
The basic format of the yeast-two hybrid system involves the creation of two hybrid molecules, one in which the " bait ' protein is fused with a transcription factor, and one in which the " prey " protein is fused with a related transcription factor . (genomicglossaries.com)
Before this stage, the pre-RNA world may have been based on molecules called xeno nucleic acids (XNAs). (astrobiology.com)

nonglycosolated virion envelope protein (24).The complete nucleotide sequence for the RNA genome of yellow fever virus has been determined (24). (cdc.gov)
On April 14, 2003, the Centers for Disease Control and Prevention (CDC) announced the completion of the full-length genetic sequencing of the genome of the SARS-associated coronavirus (SARS-CoV). (cdc.gov)
SNP calling from RNA-seq data without a reference genome: identification, quantification, differential analysis and impact on the protein sequence. (cdc.gov)
Whole-genome sequencing reveals transmission of Staphylococcus aureus from humans to green monkeys in The Gambia. (cdc.gov)
Full-Genome Sequence of a Neuroinvasive West Nile Virus Lineage 2 Strain from a Fatal Horse Infection in South Africa. (cdc.gov)
Increasingly, whole genome sequencing is being used with other analytic measures (DNA methylation, RNA expression, metabolite profiles) in large-scale collaborative initiatives. (cdc.gov)

Specifically, we used word embedding algorithm to extract features of RNA sequences and secondary structures, i.e., distributed representation of k-mers sequence rather than traditional one-hot encoding. (biomedcentral.com)
The distributed representations of RNA sequences and secondary structures can effectively detect the latent relationship and similarity between k-mers, and thus improve the predictive performance. (biomedcentral.com)
The researchers also showed that hp-sgRNAs increased the specificity of gene editing using five different Cas9 or Cas12a variants, demonstrating that RNA secondary structures can tune the activity of diverse CRISPR systems. (genomeweb.com)
Protein analyses examine the primary structure (amino acids) as well as the secondary folded structure, more complex tertiary structures, and protein interactions. (jax.org)
mRNA localisation requires primary sequences and secondary structures often localised in their 3'UTR (3). (europa.eu)
1. Describe the current algorithms and data structures used in bioinformatic sequence analysis. (aber.ac.uk)
Protein structures at protein data bank (PDB). (lu.se)

They are isolated via SELEX (Systematic Evolution of Ligands by Exponential Enrichment), an evolutionary process that involves iterative rounds of selection and amplification before sequencing and aptamer characterization. (genomicglossaries.com)
Characterization of VP1 sequence of Coxsackievirus A16 isolates by Bayesian evolutionary method. (cdc.gov)

Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins. (wikidata.org)
RNA codons and protein synthesis. (crossref.org)
When a defined protein/peptide (or combinations thereof) control and define the synthesis of an inorganic nanoparticle, the result is a cloneable NanoParticle (cNP). (rsc.org)
RNA sequences that serve as templates for protein synthesis. (bvsalud.org)

Nucleotide sequence of cucumber-mosaic-virus RNA 2 reveals a translation product significantly homologous to corresponding proteins of other viruses. (wikidata.org)

Recently, along with the rapid development and cost reduction of next generation sequencing (NGS), sequence-based assays of transcriptomes, namely RNA sequencing (RNA-seq), have become a comprehensive and accurate tool for gene expression pattern analyses. (biomedcentral.com)
Bioinformatic analyses of DNA- and protein sequences. (lu.se)

Its longest isoform consists of 4 exons, a coding sequence of 1881 nucleotides, and an upstream in-frame stop codon. (wikipedia.org)
This isofrom has a mRNA sequence of 1343 nucleotides. (wikipedia.org)
Primers were designed to target sequences in the nodavirus capsid protein gene, yielding an amplification product of 120 nucleotides. (int-res.com)
XNAs are formed of chains of linked nucleotides, similar to DNA and RNA but with a different sugar backbone. (astrobiology.com)
Both RNA and DNA are made up of a chain of building blocks called nucleotides, but they have slightly different chemical properties. (medlineplus.gov)
The mRNA interacts with a specialized complex called a ribosome, which "reads" the sequence of mRNA nucleotides. (medlineplus.gov)
Each sequence of three nucleotides, called a codon, usually codes for one particular amino acid. (medlineplus.gov)
Protein assembly continues until the ribosome encounters a "stop" codon (a sequence of three nucleotides that does not code for an amino acid). (medlineplus.gov)

RNA binding proteins (RBPs) play a vital role in post-transcriptional processes in all eukaryotes, such as splicing regulation, mRNA transport, and modulation of mRNA translation and decay. (biomedcentral.com)
RNA sequencing is beginning to show promise for understanding how a DNA variant may disrupt the normal transcription to RNA, post-transcriptional modifications, or the subsequent protein structure. (jax.org)
We show that human and chimpanzee cells differentiate in a similar man¬ner and that the difference in interspecies protein abundance is higher than transcript-level differences, suggesting that post-transcriptional mechanisms play a role in the difference between human and chim¬panzee brain development. (lu.se)

Then get on to how they typically interact with each other and how the peptide bond folds and how proteins fold and how this makes them work and some examples of them working. (physicsforums.com)

As is shown in Table 1 , these methods mostly employ RNA sequence and structural information to predict protein-RNA interactions. (biomedcentral.com)
Next, we wanted to determine miRNA sequence or structural features predictive of adapter ligation efficiencies. (rawveronica.com)
RNA testing looks for changes in gene expression, and protein analysis assesses structural changes in the protein product of the gene. (jax.org)
Natural systems also fail and pathologies result from chemical errors (e.g. sequence variants, environmental changes), structural errors, and impaired functions. (cecam.org)

Is the Subject Area "Protein structure prediction" applicable to this article? (plos.org)
Most of the existing computational approaches employed only the sequence context of the target residue for its prediction. (nature.com)
FRET X will generate higher resolution data than standard FRET, and these will then be compared with protein databases for sequence prediction. (tudelft.nl)

POF protein decorates chromosome 4 by spreading out from an initiation site in the proximal region of the chromosome. (the-scientist.com)
POF complexes may function like the Male Lethal Specific proteins, which regulate dosage-compensation of the X chromosome. (the-scientist.com)

Immunoglobulin gene transcription is activated by downstream sequence elements. (wikidata.org)
Therefore, a reliable identification of DNA-binding sites in DNA-binding protein is important for protein function annotation, in silico modeling of transcription regulation and site-directed mutagenesis. (nature.com)
Importantly, vaccinated animals were protected against intranasal challenge with IDV [3 x 10(5) 50% tissue culture infective dose(s) (TCID50)] D/OK (n = 6) or D/600 (n = 6), based on the absence of viral RNA in necropsied tissues (5 and 7 days postchallenge) using quantitative reverse transcription-PCR and in situ hybridization. (cdc.gov)
Transcription in eukaryotes is carried out by three main RNA polymerases: Pol I, II, and III. (mdpi.com)

We find that the fission yeast homologues of Tristetraprolin/TTP and Pumilio/Puf (Zfs1 and Puf3) interact with Ccr4-Not via multiple regions within low-complexity sequences, suggestive of a multipartite interface that extends beyond previously defined interactions. (elifesciences.org)
Oli [ 16 ] uses k-mer frequency as input feature into an SVM classifier to predict RNA-protein interactions. (biomedcentral.com)
Deciphering protein–protein interactions. (crossref.org)
Protein-DNA interactions are involved in many fundamental biological processes essential for cellular function. (nature.com)
An analysis of the number of binding sites in the spatial context of the target site indicates that the interactions between binding sites next to each other are important for protein-DNA recognition and their binding ability. (nature.com)
co-immunoprecipitation Used to determine protein- protein interactions . (genomicglossaries.com)

The Astros systems were developed for research into biological processes, including DNA sequencing, direct RNA sequencing, protein translation, and ligand binding. (genomeweb.com)
In order to fully understand biological processes, it is critical to be able to identify and quantify the proteins in cells. (tudelft.nl)

1 Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Institute of Life and Health Engineering, College of Life Science and Technology, Jinan University , Guangzhou 510632, China. (nih.gov)
However, for a discrete number of patients whose results are uninformative, it may be possible to get a more definitive answer about the consequences of a variant using additional methods like RNA sequencing, protein analysis and predictive or functional studies. (jax.org)
The predicted effects of a variant on functional areas of the protein such as binding sites for DNA or other proteins can also be analyzed. (jax.org)
FASSO: An AlphaFold based method to assign functional annotations by combining sequence and structure orthology. (usda.gov)

Predicting tissue-specific mRNA and protein abundance in maize: A machine learning approach. (usda.gov)
More recently, another group of researchers reported that they had developed a process for optimizing low- abundance RNA, by combining aRNA amplification with template- switching. (genomicglossaries.com)

Furthermore, this strategy also significantly improves the peptides coverage of the protein amino acid sequence. (nih.gov)
There are no internal repeats in the amino acid sequence of TMEM212. (wikipedia.org)
This can be done by sequencing: the determination of the amino-acid sequence of a protein since each protein has a unique amino acid sequence. (tudelft.nl)

the creation of a large number of compounds (usually proteins or peptides) through technologies such as phage display . (genomicglossaries.com)

Several experimental techniques have been proposed to identify the DNA-binding sites and investigate the interaction modes between proteins and DNAs. (nature.com)

RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
The knowledge about DNA-binding residues, binding specificity and binding affinity helps to not only understand the recognition mechanism of protein-DNA complex, but also give clues for protein function annotation. (nature.com)
NEW YORK (GenomeWeb) - Duke University researchers reported today in a new study that they were able to increase the specificity of CRISPR-based gene editing by several orders of magnitude by engineering a hairpin secondary structure onto the spacer region of single guide RNAs (hp-sgRNAs) and combining them with various CRISPR effectors. (genomeweb.com)
The researchers also found that hp-sgRNAs increased the specificity of gene editing with SaCas9 and AsCas12a proteins as well. (genomeweb.com)

Mutations affecting zipcodes, RBPs or motor-proteins required for neuronal mRNA localization were shown to lead to severe neurodegenerative diseases as ALS, FXTAS and FXS (7), underlining the need to understand the mechanisms that drive neuronal mRNA transport. (europa.eu)
Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. (genomicglossaries.com)
Epigenetic and RNA-mediated mechanisms. (lu.se)

It is still not known which RBPs or adaptor proteins are essential to recruit microtubule-binding motor proteins to mRNPs and to which extend this varies between different transported mRNAs. (europa.eu)
Also in this specific case, it is not understood how RBPs, potential adaptors and motor proteins, which are essential for the transport of CaMKIIa-mRNA are mechanistically contributing to its correct localisation. (europa.eu)

As of August 23, 2013, 422, 823, and 648 QTLs for milk yield, milk protein, and milk fat have been reported via marker-QTL linkage analysis (LA) and/or linkage disequilibrium (LD) ( http://www.animalgenome.org/cattle/ ). (biomedcentral.com)
Sequencing of little RNA cDNA libraries is an important tool for the discovery of new RNAs and the analysis of their mutational status as well as expression changes across samples. (rawveronica.com)
2. Perform an analysis of a set of DNA sequences and interpret the results. (aber.ac.uk)
Network Analysis of UBE3A/E6AP-Associated Proteins Provides Connections to Several Distinct Cellular Processes. (nih.gov)
sequence analysis shows it to be Hsp90? (usgs.gov)
Medicare Administrative Contractors (MACs) may determine coverage of diagnostic lab tests using NGS for RNA sequencing and protein analysis. (cms.gov)
We begin with an introduction to bioinformatics and omics data analysis, and conclude with the walkthrough of a simple bioinformatics workflow for aligning transcriptomic sequences with genomic data. (r-bloggers.com)
Methods for gene identification and analysis of gene structure: cloning, PCR, restriction mapping, in situ hybridisation, DNA sequencing. (lu.se)
The work presented in this thesis focuses on the bioinformatic methods for a TE-centric analysis of next- and third-generation sequencing technologies. (lu.se)

The mRNAs can carry short sequences of genetic letters that can trigger their own destruction. (elifesciences.org)
The test-tube system confirmed previous suggestions that a protein called Puf3 forms a bridge between Ccr4-Not and mRNAs. (elifesciences.org)
Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. (bvsalud.org)

We used the cDNA to develop an RNA (Northern) blot to quantify cellular Hsp90 mRNA levels. (usgs.gov)
Small sample size, with the high number of cellular proteins, and the necessity to distinguish 20 different amino acids are only a few of the obstacles currently limiting our ability to sequence proteins. (tudelft.nl)

Lee (2020) and Frésard (2019) demonstrated clinical utility in adding RNA sequencing to genomic testing for cohorts of individuals with undiagnosed Mendelian disease, increasing the diagnostic yield by 18% and 7.5%, respectively. (jax.org)

A comprehensive SARS-CoV-2-human protein-protein interactome reveals COVID-19 pathobiology and potential host therapeutic targets. (nih.gov)
This study reveals a previously unexplored potential of AEC to directly contribute to ECM turnover by producing interstitial ECM proteins, motivating a re-evaluation of the role of AEC2 in pathological lung remodelling. (lu.se)

GraphProt [ 14 ] adopts the form of graph coding to integrate RNA sequence and structure into the graph kernel to generate a feature vector of more than 3,000 dimensions, which is subsequently used as the input of an SVM model to predict RBP binding preference. (biomedcentral.com)
We used massive, parallel, high-throughput, RNA-seq to generate the bovine transcriptome from the mammary glands of four lactating Holstein cows with extremely high and low phenotypic values of milk protein and fat percentage. (biomedcentral.com)
Any DNA, RNA, or protein sequence can be used to generate a phylogenetic tree. (thetech.org)

Based on the detection of cell culture-derived nodavirus, and a synthetic RNA target, the real-time NASBA procedure was approximately 100-fold more sensitive than single-tube RT-PCR. (int-res.com)

Transmembrane protein 212 is a protein that in humans is encoded by the TMEM212 gene. (wikipedia.org)
Table 1: TMEM212 Protein Characteristics in Humans and Orthologs Phyre 2 and Ali2D predict TMEM212 to have a secondary structure rich in alpha helices, specifically in the transmembrane regions. (wikipedia.org)
TMEM212 is less abundant than most proteins in humans. (wikipedia.org)

Persons interested in viewing published GenBank information on SARS-CoV (Urbani strain) sequences may do so at the website of the National Center for Biotechnology Information, National Library of Medicine www.ncbi.nlm.nih.gov . (cdc.gov)

Transposable elements (TEs) are mobile genetic sequences that comprise around 50% of our genomic DNA. (lu.se)

This is shown on the right where the divergence rates of different proteins are compared. (wikipedia.org)

TMEM212 is evolving moderately quickly compared to reference sequences Cytochrome C and Fibrinogen alpha. (wikipedia.org)

In this paper, we implemented a novel deep neural network model, DeepRKE, which combines primary RNA sequence and secondary structure information to effectively predict RBP binding sites. (biomedcentral.com)
For instance, RNAcontext [ 12 ] uses position weight matrix (PWM) of RNA sequence and secondary structure profile to predict the binding preference of RBP. (biomedcentral.com)
IONMF [ 15 ] proposes a feature representation method of orthogonal matrix eigendecomposition, which integrates the k-mer sequence, secondary structure, gene ontology (GO) information and region type as input into a machine learning model to predict binding sites. (biomedcentral.com)
They hypothesized that engineering the sgRNA might serve to regulate diverse CRISPR systems, and they specifically engineered an RNA secondary structure onto the spacer by extending a designed hairpin on the 5' end of the sgRNA. (genomeweb.com)
We hypothesized that by adjusting the strength of the secondary structure, R-loop formation could proceed to completion at the on-target site but could be impeded at off-target sites, which have reduced energetics due to RNA-DNA mispairing. (genomeweb.com)
However, within the small set of miRNAs analyzed above, there was Psoralen no obvious sequence, secondary structure, or expected free energy switch correlating with the ligation effectiveness (Supplemental Table 4). (rawveronica.com)

These proteins were identified experimentally through co-expression or affinity capture-RNA. (wikipedia.org)

Scheme from the reactions for the tiny RNA cDNA collection planning. (rawveronica.com)
In summary, we have defined the 5- and 3-adapter ligation reactions as the essential steps in introducing biases in miRNA cDNA sequence go through representation. (rawveronica.com)
Researchers at Stanford University used such a method to produce amplified heterogeneous populations of RNA from limited quantities of cDNA. (genomicglossaries.com)
Amplified RNA synthesized from limited quantities of heterogeneous cDNA. (genomicglossaries.com)
They also found that the amplicons were similar in size distribution to the parent cDNA and showed sequence heterogeneity. (genomicglossaries.com)

The protein consists of 5 transmembrane domains and localizes in the plasma membrane and endoplasmic reticulum. (wikipedia.org)
In cells, membrane fusion is mediated by SNARE proteins, whose activities are calcium-dependent. (rsc.org)
COPD-derived AECs proliferated as healthy AECs with few differences in gene and protein expression while retaining increased expression of disease marker HLA-A. The AEC2 expressed basement membrane components and a complex set of interstitial ECM proteins. (lu.se)

Especially, deep learning is increasingly used in the bioinformatics field by virtue of its ability to learn generalized representations from DNA and protein sequences. (biomedcentral.com)

The proteins, called Pumilio-based assembly (Pumby) proteins, can also incorporate a translation initiator to enhance translation. (genengnews.com)
Scientists at MIT report that they have developed a building-block approach that simplifies the assembly of RNA-binding proteins. (genengnews.com)
With this approach-which the scientists call Pumby, for Pumilio-based assembly-it is possible to not only attach fluorescent labels to selected RNA sequences, but also to enhance RNA translation. (genengnews.com)
Hoping to make the assembly of RNA-binding proteins less of a trial-and-error and more programmable task, the MIT researchers tested many amino acid combinations. (genengnews.com)
We validate that the Pumby architecture can perform RNA-directed protein assembly and enhancement of translation of RNAs," the article's authors continued. (genengnews.com)

Results on the PDNA-62 and the PDNA-224 datasets demonstrate that features extracted from spatial context provide more information than those from sequence context and the combination of them gives more performance gain. (nature.com)

This program converts protein sequence into four values respectively, to be used to analyze the RNA virus protease. (mathworks.com)

Pooled-matrix protein interaction screens using Barcode Fusion Genetics. (nih.gov)

Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)
We further demonstrate a new use of such RNA-binding proteins, measurement of RNA translation in living cells. (genengnews.com)

Pumby adapts naturally occurring proteins called Pumilio homology domains (PumHDs), which consist of amino acids that bind to each of the four different ribonucleotide bases that make up RNA sequences. (genengnews.com)

Ultimately, the researchers found a particular set of amino acids that will bind each of the four bases at any position in the target sequence. (genengnews.com)
The article described how Pumby modules can be "concatenated in chains of varying composition and length to bind desired target RNAs. (genengnews.com)
First, they designed two Pumby proteins that would bind to adjacent RNA sequences. (genengnews.com)
Some researchers have reported that XNAs containing specific sequences can act as enzymes and bind to proteins. (astrobiology.com)

In contrast, animals that received a sham DNA vaccine (n = 12) had no detectable neutralizing antibodies against IDV, and viral RNA was readily detectable in respiratory tract tissues after intranasal challenge [3 x 10(5) TCID50] with IDV D/OK (n = 6) or D/660 (n = 6). (cdc.gov)
Viral RNA was isolated by guanidine thiocyanate lysis followed by purification on silica particles. (int-res.com)
All of the sequence, except for the leader sequence, was derived directly from viral RNA. (cdc.gov)

Highly specific and effective gene silencing of any disease can be achieved by an accurate knowledge of the target mRNA sequence and rational design of its complementary antisense agents for the downregulation of its protein message. (genomicglossaries.com)

RNA binding proteins recognize these sequences and assemble with their mRNA target into mRNPs. (europa.eu)

TMEM212 RNA is expressed lowly and ubiquitously in most tissue types (GDS1096). (wikipedia.org)

Anatomy8

Organisms20

Diseases1

Chemicals and Drugs84

Analytical, Diagnostic and Therapeutic Techniques and Equipment35

Phenomena and Processes83

Disciplines and Occupations4

Technology, Industry, Agriculture1

Information Science32

Health Care7

Polymerase6

Genes11

Mass Spectrometry2

Molecule10

Molecular8

Molecules9

Genome6

Structures7

Characterization2

Synthesis4

Nucleotide sequence1

Analyses2

Nucleotides8

Post-transcriptional3

Peptide1

Structural4

Prediction3

Chromosome2

Transcription4

Interactions6

Biological processes2

Functional4

Abundance2

Amino Acid Seq3

Peptides1

DNAs1

Specificity4

Mechanisms3

RBPs2

Analysis9

MRNAs3

Cellular2

Cohorts1

Reveals2

Generate3

Synthetic1

Humans3

GenBank1

Genetic sequences1

Different proteins1

Reference sequences1

Secondary6

Affinity1

CDNA5

Membrane3

Bioinformatics1

Assembly5

Datasets1

Analyze1

Genetics1

Demonstrate2

Homology1

Bind4

Viral3

MRNA sequence1

Assemble1

Tissue1