• We find that indeed the KOW domain of Mtr4 is able to bind in vitro transcribed tRNA iMet , suggesting it might assist in presenting RNA substrates to the helicase core. (pnas.org)
  • Here, we present a massively parallel RNA assay ( MPRNA ) that can assay 10,000 or more RNA segments for RNA ‐based functionality. (embopress.org)
  • MPSS (massively parallel signature sequencing) and PARE (parallel analysis of RNA ends) data retrieved from public databases were utilized, focusing on one-base-conversion editing. (biomedcentral.com)
  • In recent work, postdoctoral researcher Toomas Silla from Torben Heick Jensen's laboratory at the Department of Molecular Biology and Genetics discovered that abolished RNA exosome function causes the accumulation of polyadenylated (pA+) RNAs in distinct nuclear foci (coined as pA+ foci), indicating that these accumulated RNAs are prevented from entering the cytoplasm. (phys.org)
  • This hinted that so-called exosome adaptors, which are required for RNA exosome loading onto its substrates, might dually serve as RNA nuclear retention factors and counteract export activity. (phys.org)
  • Previously, the Torben Heick Jensen laboratory established the Nuclear EXosome Targeting (NEXT) complex and PolyA eXosome Targeting (PAXT) connection to be adaptor complexes that link the RNA exosome to short unprocessed or to longer polyadenylated RNAs, respectively (Lubas et al. (phys.org)
  • Mtr4 is a conserved RNA helicase that functions together with the nuclear exosome. (pnas.org)
  • Vertebrate NPCs, which display an eightfold rotational symmetry in electron micrographs, are large,∼ 125 MDa structures embedded in the nuclear membrane. (biologists.org)
  • NPCs feature eight fibrils that extend ∼50 nm into the cytoplasm and also feature a nuclear basket-like structure that extends up to 100 nm into the nucleoplasm. (biologists.org)
  • ARS2 directly interacts with CBP20/CBP80 to bridge interactions with several different complexes that determine RNA fate. (phys.org)
  • No genomic DNA contamination in cytoplasmic fraction - Purified cytoplasmic RNA can be used directly in RT-PCR reactions with no DNase treatment required. (biocat.com)
  • For example, it may be preferable to isolate only mature,cytoplasmic RNA for some studies on expression profiling. (biocat.com)
  • Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA. (biocat.com)
  • The kit is supplied with sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations. (biocat.com)
  • The lysate is then separated through centrifugation, with the supernatant containing the cytoplasmic RNA and the pellet containing the nuclear RNA. (biocat.com)
  • Effective Separation of HeLa Cell Cytoplasmic & Nuclear RNA. (biocat.com)
  • Norgen's Cytoplasmic & Nuclear RNA Purification Kit was used to effectively separate cytoplasmic and nuclear RNA from 0.75 million HeLa cells in triplicate. (biocat.com)
  • Panel A: High quality cytoplasmic and nuclear RNA was purified using Norgen´s kit. (biocat.com)
  • Note the integrity and quality of both cytoplasmic and nuclear RNA. (biocat.com)
  • Lane M is Norgen´s 1 kb RNA Ladder, lanes 1-3 contain nuclear RNA and lanes 4-6 contain cytoplasmic RNA. (biocat.com)
  • Panel B: Ten microliters of the above cytoplasmic and nuclear RNA isolated from HeLa cells using Norgen´s kit was run on a 0.9% agarose DNA gel. (biocat.com)
  • Genomic DNA is clearly visible in the nuclear RNA fractions (lanes 1-3), however, no genomic DNA can be detected in the cytoplasmic RNA fractions (lanes 4-6). (biocat.com)
  • Genomic DNA-free Cytoplasmic RNA. (biocat.com)
  • RT-PCR was performed using human beta actin primers on 2 µL of the 50 µL of cytoplasmic RNA isolated from 1 million HeLa cells using Norgen´s Cytoplasmic & Nuclear RNA Purification Kit. (biocat.com)
  • No relationship of precursor-to-product type -- or, at any rate, not a simple one-appears to exist between this RNA and the messenger RNA fraction associated with cytoplasmic polysomes. (caltech.edu)
  • Senescent human fibroblasts have a post-transcriptional block in the expression of the proliferating cell nuclear antigen gene. (semanticscholar.org)
  • The bulk of cellular nucleocytoplasmic transport is mediated by factors that belong to a single family of nuclear transport receptors termed karyopherins or importins/exportins. (biologists.org)
  • Tap/NXF1 is the metazoan nuclear export receptor for both poly (A+) RNA as well as cellular and viral RNAs containing the constitutive transport element (CTE). (illinois.edu)
  • Historically, investigation of Polyomavirus replication allowed identification ofsequences that mediate nuclear import, which led subsequently to our detailed understanding of the cellular factors that are involved in nuclear import. (waterstones.com)
  • The investigation of retroviral gene expression in recent years pro- vided the first insights into the cellular mechanisms that regulate nuclear export. (waterstones.com)
  • All non-coding RNAs are now known to depend on members of the karyopherin family of Ran-dependent nucleocytoplasmic transport factors for their nuclear export. (biologists.org)
  • The 2014 IMB Meeting on Nuclear RNA, Gene Regulation, and Chromatin Structure took place from the 9th till the 12th of October at the Institute for Molecular Biology (IMB) in Mainz, Germany. (epigenie.com)
  • May be required for packaging pre-mRNAs into ribonucleoprotein structures amenable to efficient nuclear RNA processing. (mybiosource.com)
  • These results imply that speckle size may be regulated to accommodate RNA accumulation and processing. (biologists.org)
  • The majority of cDNAs produced from poly(A)-selected RNA isolated from a human body cavity lymphoma cell line 48 hr after butyrate induction of KSHV lytic replication represented PAN RNA. (pnas.org)
  • It has been long understood that the NXF family is unique from the Karyopherin transport receptor family and that RNA export functions independently of the nuclear Ran-GTP gradient. (illinois.edu)
  • The requirement of the Tap complex for successful export implies that nuclear regulation of Tap complex association and dissociation is necessary prior to exit with cargo. (illinois.edu)
  • Mammalian genomes produce a large repertoire of RNAs, which experience very different fates. (phys.org)
  • Transcriptome-wide characterization of pA+ RNA foci contents, revealed surprisingly many full-length and spliced mRNAs. (phys.org)
  • Multimeric Tap is capable of forming interactions with the factors necessary for export but does not prefer binding RNA in the multimeric form, suggesting that multimeric Tap is not actively exporting cargo. (illinois.edu)