• Based on sequence and structural motifs, 113 of these RNAs can be assigned to the C/D box or H/ACA box subclass of small nucleolar RNAs (snoRNAs), known as guide RNAs for rRNA. (embopress.org)
  • Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes, Nature Communications (2018). (phys.org)
  • The primary RNA transcript of a gene is cleaved at the poly-A addition site, and 100-200 A's are added to the 3' end of the RNA. (wikipedia.org)
  • Triton X-100 extraction of follicle cells yielded a filamentous CF containing approximately 70% of the total poly (A) but only 9% of the total lipid, 23% of the total protein, and 28% of the total RNA. (rupress.org)
  • Total RNA from 1 X 10(7) cells was extracted, transferred to a membrane, and hybridized with a 32P-labeled, full-length (1650-base pair) rat angiotensinogen complementary DNA (cDNA). (ahajournals.org)
  • That the RNA was intact was ensured by hybridizing duplicate Northern blots to a 32P-labeled actin cDNA. (ahajournals.org)
  • Aliquots containing 5 μg of total cellular RNA were reverse transcribed, and first-strand cDNA was used as a template in polymerase chain reaction (PCR). (ahajournals.org)
  • Five micrograms of RNA was reverse transcribed into cDNA and l- μ g aliquots subjected to polymerase chain reaction (PCR) amplification for 25 cycles using 32 P-labeled primers specific for rat cytokines and glyeeraldehyde-3-phosphate dehydrogenase (G3PD), a housekeeping gene. (pubmedcentralcanada.ca)
  • Utzinger, Maximilian (2017): PLGA-based micro- and nanoparticle development for chemically modified messenger RNA delivery. (uni-muenchen.de)
  • Although the concepts presented are applicable to all types of RNA, we showcase here the wealth of information gained from in vivo imaging of single particles by discussing studies investigating dynamics of intranuclear trafficking, nuclear pore transport and cytoplasmic transport of endogenous messenger RNA. (biologists.org)
  • Despite a rich body of literature on these events, an involvement of nuclear RNA decay systems remains largely unexplored. (readbyqxmd.com)
  • Among these, for the first time, we identified four C/D box snoRNAs and four H/ACA box snoRNAs predicted to direct modifications within U2, U4 or U6 small nuclear RNAs (snRNAs). (embopress.org)
  • The transcribed RNA now represents information required to direct cellular function to maintain cell homeostasis. (mdpi.com)
  • This volume is devoted to current studies in the field of cellular and viral messenger RNA. (springer.com)
  • Soukup, G. A. & Breaker, R. R. Engineering precision RNA molecular switches. (nature.com)
  • The work is a collaboration between the Verma lab and Arcturus Therapeutics, a local biotech company that developed a system of encapsulating messenger RNA within lipid (fatty acid) nanoparticles. (salk.edu)
  • Soukup, G. A. & Breaker, R. R. Relationship between internucleotide linkage geometry and the stability of RNA. (nature.com)
  • DNA and RNA oligonucleotides can be modified for stability as well as engineered to contain inherent cleaving activity. (elsevier.com)
  • Finally, the ratio of growth-associated protein-43 messenger RNA to synaptophysin messenger RNA--a putative index of the production of new synapses--was decreased in the medial temporal lobe in the schizophrenics. (ox.ac.uk)
  • Such protein-RNA complexes are resistant to the actions of denaturing and reducing agents, demonstrating very stable binding. (sciencemag.org)
  • The structure consists of a five-stranded antiparallel β-barrel, a structural motif known to bind RNAs ( 15 ). (pnas.org)
  • In the schizophrenia group, growth-associated protein-43 messenger RNA was decreased in the medial temporal lobe, primary visual cortex and anterior cingulate gyrus, but was unaltered in the superior temporal and dorsolateral prefrontal cortices. (ox.ac.uk)
  • The missense mutations G207D and R229P did not show NMD although R229P exhibited moderate RNA instability. (diabetesjournals.org)