• The sequence of the nucleosides is such that they will form a self‐complementary double‐helical fragment which may serve as a probe to get more insight into the process of discontinuous DNA replication. (tmu.edu.tw)
  • Within virions, the genomic RNAs of hantaviruses are thought to complex with the N protein to form helical nucleocapsids, which circularize due to sequence complementarity between the 5′ and 3′ terminal sequences of each genomic segment. (cdc.gov)
  • (a) What is the corresponding complementary DNA sequence? (ubc.ca)
  • A sensor sequence is then designed as the target RNA's complementary strand. (eurekalert.org)
  • After a sensor makes its way into a cell and finds its target RNA sequence, both pieces glom together to create a piece of double-stranded RNA. (eurekalert.org)
  • The stop sign, which prevents the protein blueprints from being built, is only removed once CellREADR's sensor docks to its target RNA sequence, making it highly specific for a given cell type. (eurekalert.org)
  • a complex of an RNA complementary in sequence to DNA. (nih.gov)
  • Reverse transcription is the synthesis of a complementary DNA sequence from an RNA template using reverse transcriptase, which is an RNA-dependent DNA polymerase. (medscape.com)
  • Applied to 106 ENCODE human RNA-seq datasets, ChimPipe identified 137 high confidence chimeras connecting the protein coding sequence of their parent genes. (biorxiv.org)
  • Within the complex, microRNAs are thought to bind to mRNA molecules containing a complementary stretch of RNA sequence. (eurekalert.org)
  • mRNAs corresponding to several regulatory genes that mediate auxin responses contain short stretches of sequence that are complementary to microRNAs, and therefore have been considered potential targets of microRNA-mediated regulation. (eurekalert.org)
  • The use of engineered RTs improves the efficiency of full-length product formation, ensuring the copying of the 5′ end of the mRNA transcript is complete, and enabling the propagation and characterization of a faithful DNA copy of an RNA sequence. (neb.com)
  • For example, in the nucleotide sequence surrounding a given target, secondary structures in the RNA can reduce target-site accessibility and reduce the chance of transcript regulation by the miRNA. (genengnews.com)
  • Dr. Brown and his colleagues found that they could prevent this response by modifying the 3´ UTR of a vector to contain four tandem copies of a sequence that is perfectly complementary to miR-142, an miRNA highly expressed in APCs but not in hepatocytes. (genengnews.com)
  • Such conjugates can be used as universal artificial RNA scissors without sequence restrictions for all kinds of analytical purposes. (uni-frankfurt.de)
  • A RISC complex charged with a specific miRNA (symbolized here by red color) would bind to the complementary "red" RNA sequence, not present in the mRNA example shown above. (uni-frankfurt.de)
  • The second part is complementary to the "blue" sequence of the mRNA. (uni-frankfurt.de)
  • the PNA part (peptide nucleic acids) ensures sequence specific hybridization with the target and the quinone methide (highlighted in yellow) as a potent electrophile reversibly alkylates nucleophilic sites within the RNA (RNA nucleotides shown in black, PNA nucleotides in magenta). (uni-frankfurt.de)
  • First, the gene's DNA sequence is copied into RNA, a similar but less stable molecule. (nih.gov)
  • The ribosomes match the tRNAs to their complementary mRNA sequences, thus "reading" the mRNA sequence, and stitch the amino acids together to make proteins. (nih.gov)
  • The high affinity of an LNA-enhanced oligonucleotide to its complementary sequence results in dramatically improved specificity and sensitivity, when compared with traditional DNA or RNA oligos. (qiagen.com)
  • A short sequence of single stranded DNA or RNA. (kaiserpermanente.org)
  • Here, a tRNA molecule, with a three-nucleotide anticodon sequence on one side and a specific amino acid on the other, binds to a complementary codon in the mRNA. (jove.com)
  • RNA is extremely flexible because it has sequence. (nih.gov)
  • they just need to contain a complementary sequence. (nih.gov)
  • This synthetic DNA is produced from the molecule that serves as the instructions for making proteins (called messenger RNA). (medlineplus.gov)
  • Unlike the large messenger RNA (mRNA) molecules that code for cellular proteins, the tiny microRNAs regulate gene activity by interfering with mRNAs. (news-medical.net)
  • Messenger RNA (mRNA) molecules are encoded by genes and are themselves templates for the proteins that carry the main metabolic functions in a cell. (eurekalert.org)
  • There are many AGO1-like proteins in animals and other eukaryotes as well, indicating that the RNA-induced silencing complex is of ancient evolutionary origin, and that microRNA-mediated regulation of gene expression is shared among many eukaryotes. (eurekalert.org)
  • A. Micro RNAs (miRs) are embedded in Argonaute proteins to form RISC complexes (RNA induced silencing complex). (uni-frankfurt.de)
  • Crosslinking of RNA to complementary oligonucleotides is the chemical background of important bioanalytical protocols and may also help to recruit and redirect Argonaute proteins charged with specific micro RNAs (see above). (uni-frankfurt.de)
  • This "messenger RNA" (mRNA) travels to the ribosome, the molecular machine that synthesizes proteins. (nih.gov)
  • The central dogma of molecular biology states that DNA contains the information that encodes proteins, and RNA uses this information to direct protein synthesis. (jove.com)
  • Non-coding RNAs other than tRNA and rRNA were initially considered to be "genomic junk" since they did not encode proteins. (jove.com)
  • Pieces of RNA are used to construct proteins inside of your body so that new cell growth may take place. (study.com)
  • The other main function of RNA is to select the correct amino acid needed by each ribosome to build new proteins for your body, with an amino acid being one ingredient used to make a protein. (study.com)
  • Long ncRNAs (lncRNAs) are the most prevalent class of non-coding RNAs (ncRNAs) with more than 200 nucleotides. (biomedcentral.com)
  • As the RNA polymerase continues down the strand of DNA, more nucleotides are added to the mRNA, thereby forming a progressively longer chain of nucleotides (Figure 2). (github.io)
  • Finally, nucleotides complementary to the target DNA are added extending each primer by the thermostable DNA polymerase. (medscape.com)
  • MicroRNA (miRNA) is a class of small non-coding RNA with about 21-25 nucleotides in length [ 10 , 11 ]. (jcancer.org)
  • This observation finally led us to the development of phosphoramidite 1 ( A ). Compound 1 can be attached in high yield to the 5' end of DNA or LNA oligonucleotides ( B ). Panel C depicts a duplex formed by such DNA-catalyst conjugates and dye-labeled RNA substrates (deoxy-nucleotides in blue and ribonucleotides in black letters). (uni-frankfurt.de)
  • Replacing DNA nucleotides by LNA in selected positions further enhances RNA cleavage (substrate half-life about 3.5 h). (uni-frankfurt.de)
  • What is super cool is that short RNAs and the RNAs we work on are only about 25 nucleotides in length-they are really tiny. (nih.gov)
  • T]he rapid losses of nucleobases to pond seepage during wet periods, and to UV photodissociation during dry periods, mean that the synthesis of nucleotides and their polymerization into RNA occurred in just one to a few wet-dry cycles. (creation.com)
  • Therefore, the RNA must have formed very rapidly before the nucleotides were lost or destroyed. (creation.com)
  • Information from DNA is passed through messenger RNA (mRNA), which contains sets of four nucleotides (uracil, adenine, guanine, and cytosine). (biochain.com)
  • Two complementary computational screens, eQRNA and RNAz, were used to search for novel sRNA-encoding genes in the intergenic regions IGRs of S. meliloti. (wikipedia.org)
  • All hantaviral genes are encoded in the negative (genome complementary) sense. (cdc.gov)
  • DURHAM, NC - Duke University researchers have developed an RNA-based editing tool that targets individual cells, rather than genes. (eurekalert.org)
  • Many programs have recently been developed to identify chimeras from Illumina RNA-seq data (mostly fusion genes in cancer). (biorxiv.org)
  • Hannon and Lowe, who were recently appointed as HHMI investigators at Cold Spring Harbor Laboratory , say that given the new findings, they agree with a proposal calling for cancer-causing microRNAs to be dubbed "oncogenic micro RNAs," or "oncomiRs," just as cancer-causing genes are called oncogenes. (news-medical.net)
  • We obtained an average of 81 million reads per sample from the RNA-Seq data, of which 90% were retained for further analysis based on the mapping results, the vast majority (94.0%) of which localized to protein coding genes. (nature.com)
  • functional validation to determine which candidate genes/variants/epigenetic/non-coding RNA features have an authentic role in addictive processes, and 2. (nih.gov)
  • The articles produced by the PubMed search were reviewed for those specifically addressing a genetic profile's ability to predict response to neoadjuvant CRT in LARC (genes, microRNA, or long noncoding RNA). (hindawi.com)
  • This combination of reverse transcription and PCR (RT-PCR) allows the detection of low abundance RNAs in a sample, and production of the corresponding cDNA, thereby facilitating the cloning of low copy genes. (neb.com)
  • Therefore, the principal limitation of this group of methods is the incapacity to address splicing, fusion genes, or RNA editing-related research questions. (biomedcentral.com)
  • mRNA is produced when enzymes, such as RNA polymerase, bind to specific genes and copy their information into RNA using a ribose sugar (not deoxyribose as in DNA). (biochain.com)
  • A single-stranded chemiluminescent DNA probe, which is complementary to a region of the target amplicon, is labeled with different acridinium ester molecule. (cdc.gov)
  • RNA is a vital molecule found in your cells, and it's necessary for life. (study.com)
  • As an RNA biologist, RNA might have been the first molecule that enabled life. (nih.gov)
  • Researchers at the University of Tokyo have for the first time been able to create an RNA molecule that replicates, diversifies, and develops complexity, following Darwinian evolution. (creation.com)
  • The S RNA encodes the nucleocapsid (N) protein. (cdc.gov)
  • The L RNA encodes the L protein, which functions as the viral transcriptase/replicase. (cdc.gov)
  • In addition to transcriptase and replicase functions, the viral L protein is also thought to have an endonuclease activity that cleaves cellular messenger RNAs (mRNAs) for the production of capped primers used to initiate transcription of viral mRNAs. (cdc.gov)
  • The L protein produces nascent genomes by replication via a positive-sense RNA intermediate. (cdc.gov)
  • The protein structure of E. coli RNase HI unbound to a substrate is similar to that of the structure of human RNase H1 in complex with an RNA/DNA hybrid. (nih.gov)
  • MicroRNAs are ~22 nucleotide long RNA molecules that provide substrate specificity to a protein complex known as the RNA-induced silencing complex. (eurekalert.org)
  • A second report focuses on the function of the protein ARGONAUTE1 (AGO1), a major component of the RNA-induced silencing complex in Arabidopsis. (eurekalert.org)
  • miRNAs act through binding to complementary mRNA sequences, thereby preventing their translation into protein or accelerating mRNA breakdown. (genengnews.com)
  • Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). (jove.com)
  • These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. (jove.com)
  • Messenger RNA (mRNA) is the protein-coding RNA. (jove.com)
  • Thus, different types of RNA perform specific but complementary functions during protein synthesis. (jove.com)
  • The notion that information might always flow from DNA to RNA to protein was somewhat jokingly referred to as the Central Dogma of molecular biology. (biochain.com)
  • The Court specifically mentioned the ability to patent a type of DNA known as complementary DNA (cDNA). (medlineplus.gov)
  • The synthesis of DNA from an RNA template, via reverse transcription, produces complementary DNA (cDNA). (neb.com)
  • Reverse transcriptases (RTs) use an RNA template and a primer complementary to the 3′ end of the RNA to direct the synthesis of the first strand cDNA, which can be used directly as a template for the Polymerase Chain Reaction (PCR). (neb.com)
  • DNA that arises from this process is called complementary DNA (cDNA). (biochain.com)
  • Another type of RNA, called transfer RNA (tRNA), binds to amino acids. (nih.gov)
  • Transfer RNA (tRNA) and ribosomal RNA (rRNA) are non-coding RNA. (jove.com)
  • The two most important types of RNA are messenger RNA (mRNA) and transfer RNA (tRNA). (study.com)
  • At the beginning of each step, a transfer RNA (tRNA), carrying its specific amino acid attaches to the mRNA triplet. (creation.com)
  • The GEN-PROBE TMA reaction replicates a specific region of the small ribosomal subunit from trichomonas vaginalis via DNA and RNA intermediates and generates RNA amplicon molecules. (cdc.gov)
  • In contrast, ALS, MtDis and AGS have R-loops in which RNA/DNA duplexes are formed during RNA synthesis with one of the duplex strand is looped out (single stranded DNA). (nih.gov)
  • It is known that during RNA synthesis R-loops can form and that aberrant R-loop formation can result in chromosome breakage. (nih.gov)
  • Chemical Biology of Nucleic Acids: Synthesis of Functional Molecules Acting on DNA and RNA. (uni-frankfurt.de)
  • The 84th Cold Spring Harbor Symposium focused on RNA Control & Regulation and provided a current synthesis of the enormous progress in our understanding of RNA's influence on cells and organisms and how, when aberrant, its effects may contribute to the progression of disease. (cshlpress.com)
  • Each is made up of negative-sensed, single-stranded RNA viruses. (cdc.gov)
  • Like other members of the bunyavirus family, hantaviruses are enveloped viruses with a genome that consists of three single-stranded RNA segments designated S (small), M (medium), and L (large). (cdc.gov)
  • To date, more than 5,000 of these endogenous, noncoding single-stranded RNAs have been identified. (genengnews.com)
  • Just as the rungs on DNA are made-up of complementary molecules that are inherently attracted to each other, RNA has the same magnetic potential to link with another piece of RNA if it has matching molecules. (eurekalert.org)
  • The RNA molecules attach to their complementary DNA counterparts on the slide. (news-medical.net)
  • We used chimeric single-guide RNA (cgRNA) molecules carrying both sequences for target site specificity (to generate the double-strand breaks) and repair template sequences (to direct HDR), flanked by regions of homology to the target. (frontiersin.org)
  • The capture oligomers contain sequences complementary to specific regions of the target molecules as well as a string of deoxyadenosine residues. (cdc.gov)
  • Within genetics, post-genomic research has rendered bacterial small non-coding RNAs (sRNAs) as major players in post-transcriptional regulation of gene expression in response to environmental stimuli. (wikipedia.org)
  • These new genomic loci were referred to as smr, for S. meliloti RNA. (wikipedia.org)
  • Aberrations in the intimate role of RNA associated with DNA create genomic damage and, therefore formation and resolution of RNA-DNA interaction is critical. (nih.gov)
  • It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences. (nih.gov)
  • In HIV-AIDS, the RNA genome of the virus is copied as DNA forming a simple RNA/DNA hybrid. (nih.gov)
  • RNA/DNA hybrids are essential intermediates in the replication of HIV's RNA genome. (nih.gov)
  • For each nucleotide in the template, RNA polymerase adds a matching (complementary) RNA nucleotide to the 3' end of the RNA strand. (github.io)
  • RNA polymerase synthesizes an RNA transcript complementary to the DNA template strand in the 5' to 3' direction. (github.io)
  • The RNA polymerase moves stepwise along the DNA, unwinding the DNA helix just ahead of the active site for polymerization to expose a new region of the template strand for complementary base-pairing. (github.io)
  • Strand elongation By virtue of complementary base-pairing, this action creates a new strand of mRNA that is organized in the 5' to 3' direction. (github.io)
  • RNA polymerase synthesizes an RNA strand complementary to a template DNA strand. (github.io)
  • It synthesizes the RNA strand in the 5' to 3' direction, while reading the template DNA strand in the 3' to 5' direction. (github.io)
  • The template DNA strand and RNA strand are antiparallel. (github.io)
  • complementary strand. (wikipedia.org)
  • The ADAR enzyme is a cell-defense mechanism designed to edit double-strand RNA when it occurs, and is believed to be found in all animal cells. (eurekalert.org)
  • HIV-AIDS, MtDis and AGS are all affected by ribonucleases H (RNases H), enzymes that degrade the RNA strand of RNA/DNA hybrids including R-loops. (nih.gov)
  • Here, we used CRISPR/Cas9 to generate targeted double-strand breaks and to deliver an RNA repair template for HDR in rice ( Oryza sativa ). (frontiersin.org)
  • Gene editing was more efficient in rice protoplasts using repair templates complementary to the non-target DNA strand, rather than the target strand. (frontiersin.org)
  • The ribosome matches tRNAs (red, blue and bright green) to their complementary sequences in mRNA (multicolored strand below). (nih.gov)
  • We learn this now in middle school-when you have DNA form a double strand, when you have RNA or DNA strands, if they have complementary base pairs, they can actually recognize each other. (nih.gov)
  • This new RNA mashup triggers the enzyme ADAR to inspect the new creation and then change a single nucleotide of its code. (eurekalert.org)
  • Knowing this, Qian designed CellREADR's stop sign using the same specific nucleotide ADAR edits in double-stranded RNA. (eurekalert.org)
  • We undertook a comprehensive molecular evaluation of the samples using WGS, single-nucleotide polymorphism (SNP) arrays (Illumina Human660-Quad BeadChip) and transcriptomics (RNA-seq). (nature.com)
  • Notably, RNA metabolism and splicing regulation pathways were enriched at the 7-wk time point, when the greatest number of differentially alternatively spliced mRNAs are detected. (nih.gov)
  • Among these aberrantly expressed transcripts, not only mRNAs, but also many non-coding RNAs were identified [ 4 , 9 ]. (jcancer.org)
  • However, despite the magnitude of SOX targeting, we and others have shown that there remains a smaller subset of mRNAs that actively evade SOX cleavage via a protective RNA element within their 3'UTR known as a "SOX resistant element" or SRE. (umass.edu)
  • Specifically, the competing endogenous RNA (ceRNA) hypothesis was proposed to describe lncRNA-microRNA-mRNA crosstalk. (nature.com)
  • Let's discuss the two most important types of RNA to get a better idea about RNA's function inside of your body's cells. (study.com)
  • This N-terminal domain (HBD) interacts with RNA/DNA through contacts with both the DNA and RNA strands and may provide the initial contact between the enzyme and hybrid. (nih.gov)
  • Automatic recognition of complementary strands: lessons regarding machine learning abilities in RNA folding. (iric.ca)
  • Target amplification assays are based on the ability of complementary oligonucleotide primers to specifically anneal and allow enzymatic amplification of the target nucleic acid strands. (cdc.gov)
  • The vast majority of the transcripts are termed as non-coding RNAs, including microRNAs and long non-coding RNAs (lncRNAs). (nature.com)
  • That slide is then treated with a mixture of fluorescently tagged messenger RNA isolated from cells whose activity is to be analyzed. (news-medical.net)
  • For SkBr3, the estimated pharmacokinetic parameters k 1 and k 3 were consistent with the messenger RNA expression of GLUT1 and hexokinase-2: culturing with low glucose concentrations led to higher GLUT1 and hexokinase-2 expression as well as higher estimated k 1 and k 3 . (snmjournals.org)
  • For Capan-1, the estimated k 1 and k 3 increased as the glucose concentration in the culture medium decreased, and this finding did not match the corresponding messenger RNA expression. (snmjournals.org)
  • The messenger RNA (mRNA) arrives from the nucleus of the cell, carrying the information for a specific gene. (creation.com)
  • During lytic replication, Kaposi's sarcoma-associated herpesvirus (KSHV) triggers a massive RNA decay event known as viral "Host Shutoff" which decimates greater than 70% of the host transcriptome, simultaneously suppressing the cellular anti-viral response and freeing host resources for viral replication. (umass.edu)
  • In the first chapter, using RNA-seq, we identified multiple cellular mRNA capable of escaping viral host shutoff, including C19ORF66. (umass.edu)
  • In this pursuit, we have revealed novel insights into the crossroads between the host anti-viral response and the regulation of RNA fate during viral infection. (umass.edu)
  • What is the enzyme that synthesizes DNA using a viral RNA as a template? (flashcardmachine.com)
  • These things are getting closed for good, already in germ cells, by small RNA-based mechanisms. (nih.gov)
  • These kinds of mechanisms-that rely on these short guide RNAs to recognize another RNA by complementary base pairing-are called RNA interference pathways. (nih.gov)
  • Previous Cold Spring Harbor Symposia have addressed many different aspects of RNA biology such as Mechanisms of Transcription (1998), The Ribosome (2001), Epigenetics (2004) and Regulatory RNAs (2006). (cshlpress.com)
  • Then, in the second chapter, we defined two complementary mechanisms by which Shiftless inhibits KSHV lytic replication. (umass.edu)
  • Investigators have found that miRNA regulatory processes involve miRNA activation of gene expression by interacting with complementary regions found in the promoter coding region, as well as the 3´ UTR of their mRNA targets. (genengnews.com)
  • Extensive regulation of miRNA itself occurs at the levels of miRNA promoter transcription, methylation, miRNA processing, RNA editing, and miRNA-target interactions. (genengnews.com)
  • Eukaryotic RNases H have an N-terminal domain (absent from the bacterial enzyme) that binds to RNA/DNA, conferring processivity to the enzyme (Gaidamakov et al. (nih.gov)
  • The tool's remarkable tissue specificity relies on the fact that each cell type manufactures signature RNA not seen in other cell types. (eurekalert.org)
  • RNA-sequencing (RNA-Seq) was performed with poly(A) RNA isolated from cells harvested at 7, 19, and 28 wk with subsequent replicate multivariate analysis of transcript splicing (rMATS) analysis to detect and quantify differential alternative splicing events. (nih.gov)
  • However, most transcriptomics studies do not require or exploit full transcript information, implying that standard RNA-seq methods tend to generate more information than is typically required. (biomedcentral.com)
  • In our previous study we have found that miR-548k has oncogenic roles in esophageal squamous cell carcinoma (ESCC) via repressing long noncoding RNA (lncRNA)-LET and further upregulating nuclear factor 90 (NF90). (jcancer.org)
  • Only one screened long noncoding RNA (lncRNA) was associated with radiosensitivity but was made in colorectal cancer cell lines and was written in Chinese and therefore was excluded. (hindawi.com)
  • A nucleic acid fragment containing a RNA trimer and a DNA heptamer wich are linked by a 3′‐5′‐internucleotide phosphodiester bond has been synthesized by a phosphotriester approach. (tmu.edu.tw)
  • 11. RNA guanine quadruplex invasion by complementary and homologous PNA probes. (nih.gov)
  • mRNA is first transcribed from DNA by complementary base pairing in the nucleus of eukaryotic cells, with adenine binding to thymine, guanine binding to cytosine, and uracil binding to adenine. (jove.com)
  • The four RNA bases will pair together as an adenine/uracil pair and a guanine/cytosine pair. (study.com)
  • They pair with their complementary purines, guanine and adenine, through hydrogen bonding to form DNA and RNA chains. (osu.edu)
  • RNA Editing of the GP Gene of Ebola Virus is an Important Pathogenicity Factor. (nih.gov)
  • Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. (biomedcentral.com)
  • Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. (jove.com)
  • At the heart of this struggle is a contest for control of cellular gene expression, a struggle epitomized by an evolutionary tug-of-war for supremacy over RNA fate. (umass.edu)
  • Surprisingly, we also found SHFL profoundly impacts global gene translation, an effect reflected by changes in global RNA dynamics. (umass.edu)
  • The M RNA encodes a polyprotein that is cotranslationally cleaved to yield the envelope glycoproteins G1 and G2. (cdc.gov)
  • Mechanistically, transcription factor SP1 induced the transcription of linc00665 in LUAD cells, which exerted its oncogenic role by functioning as competing endogenous RNA (ceRNA) for miR-98 and subsequently activating downstream AKR1B10-ERK signaling pathway. (nature.com)
  • While these are early days for CellREADR (Cell access through RNA sensing by Endogenous ADAR), the possible applications appear to be endless, Huang said, as is its potential to work across the animal kingdom. (eurekalert.org)
  • Their technique involves attaching to color-coded plastic beads DNA sequences complementary to the individual miRNAs whose levels they wish to probe. (news-medical.net)
  • We know a great deal about the recognition of RNA/DNA hybrids and the enzymatic mechanism of hydrolysis of the RNA. (nih.gov)
  • lane 4: hydrolysis ladder of the RNA substrate. (uni-frankfurt.de)
  • 6. Hybridization of short complementary PNAs to G-quadruplex forming oligonucleotides: An electrospray mass spectrometry study. (nih.gov)
  • 15. Sole and stable RNA duplexes of G-rich sequences located in the 5'-untranslated region of protooncogenes. (nih.gov)
  • Long non-coding RNAs (lncRNAs) are frequently dysregulated in multiple malignancies, demonstrating their potential oncogenic or tumor-suppressive roles in tumorigenesis. (nature.com)
  • In this study, we set out to generate a method for affordable, efficient, and accurate bulk RNA profiling of a large number of samples that combines the high-throughput capacity of single-cell transcriptomics and the high performance of standard RNA-seq. (biomedcentral.com)
  • The use of the more thermostable RTs, where reactions are performed at higher temperatures, may be helpful for reverse transcription of RNA that containing secondary structure. (neb.com)
  • All RNA-seq library preparation methods are globally relying on the same molecular steps, such as reverse transcription (RT), fragmentation, indexing, and amplification. (biomedcentral.com)
  • In this way, during reverse transcription, information flowed backwards from RNA back to DNA. (biochain.com)
  • What general transcription factor for RNA polymerase II acts immediately after TFIID in the formation of the pre-initiation complex? (flashcardmachine.com)
  • How these enzymes recognize and cleave the RNA is important for our understanding of the biology of these diverse events and for possible regulation of RNase H activity. (nih.gov)
  • Our laboratory interested in understanding how RNA/DNA hybrids are resolved and what role RNases H play in their elimination. (nih.gov)
  • The labeled DNA probe combines with amplicon to form stable RNA: DNA hybrids. (cdc.gov)
  • During the detection step, light emitted from the labeled RNA: DNA hybrids is measured as photon signals in a luminometer, and are reported as Relative Light Units (RLU). (cdc.gov)
  • Dr. Haase is a Stadtman Tenure-Track Investigator and Acting Section Chief of the RNA Biology Section in the Laboratory of Cellular and Molecular Biology branch at NIDDK [National Institute of Diabetes and Digestive and Kidney Diseases]. (nih.gov)
  • We have this long-standing interest in trying to figure out how this small RNA-based immune system is able to recognize incoming viruses and protect us against our resident parasites. (nih.gov)