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  • Interference
  • We inhibited the expression of approximately 96% of the approximately 2,300 predicted open reading frames on chromosome III using RNA-mediated interference (RNAi). (mdc-berlin.de)
  • A cellular function for the RNA-interference enzyme Dicer in the matur" by Gyorgy Hutvagner, Juanita McLachlan et al. (umassmed.edu)
  • We present in vivo and in vitro evidence that in Drosophila melanogaster a developmentally regulated precursor RNA is cleaved by an RNA interference-like mechanism to produce mature let-7 stRNA. (umassmed.edu)
  • Targeted destruction in cultured human cells of the messenger RNA encoding the enzyme Dicer, which acts in the RNA interference pathway, leads to accumulation of the let-7 precursor. (umassmed.edu)
  • Thus, the RNA interference and stRNA pathways intersect. (umassmed.edu)
  • UDP-Galactopyranose mutase ( bmugm ) plays a key role in Gal f biosynthesis by catalyzing conversion of UDP-Galactopyranose into UDP-galactofuranose and knockout studies of the gene in Leishmania major , Mycobacterium and Aspergillus fumigatus displayed attenuated virulence while RNA interference study in C. elegans exhibited detrimental effects. (biomedcentral.com)
  • In the present study, RNA interference studies have been carried out to validate bmugm as an antifilarial drug target. (biomedcentral.com)
  • RNA interference studies using two different sequences of siRNAs targeting bmugm were carried out. (biomedcentral.com)
  • The reverse genetic approach may be of help in this regard and RNA interference (RNAi) provides a valuable modern drug discovery platform for identifying gene(s) playing vital role in parasite metabolism by inhibiting/silencing their expression. (biomedcentral.com)
  • As we discuss here, new insights into fundamental helminth biology are accumulating through newly completed genome projects and the nascent application of transgenesis and RNA interference technologies. (nih.gov)
  • Supported by the availability of gene manipulation technologies such as RNA interference and/or transgenesis, this work provides a basis for future functional explorations of helminth TIMPs and, in particular, of their role/s in fundamental biological pathways linked to long-term establishment in the vertebrate hosts, with a view towards the development of novel approaches for the control of neglected helminthiases. (biomedcentral.com)
  • Genome
  • Picobirnaviruses (PBVs) with bisegmented small RNA genome profile (1.75 and 1.55kbp for segment 1 and 2, respectively) were detected from 1999 to 2003 in faecal specimens of acute watery diarrhoea cases, largely children (n=20) and an adult in Kolkata, India. (nih.gov)
  • Today, RNA-seq data is available for a wide range of organisms, tissues and environmental conditions, and can be utilized for genome annotation pipelines. (biomedcentral.com)
  • Improving genomics resources for helminths: including third-generation sequencing technologies for improving genome assembly and annotation, novel algorithms and packages, and new bioinformatic tools. (frontiersin.org)
  • antigens
  • Consistent with a relationship between Th2 and TFH cells, IL-4 protein production, reported by expression of huCD2 in IL-4 dual reporter (4get/KN2) mice, was a robust marker of TFH cells in LNs responding to helminth antigens. (rupress.org)
  • helminths and antigens derived from them inherently induce Th2-polarized responses ( 13 ). (rupress.org)
  • Proteins
  • They are composed of proteins, lipids, and RNAs through which they participate in cellular crosstalk. (frontiersin.org)
  • Comparative genomics are highlighting the common presence of helminth exclusive conserved hypothetical proteins, challenging researchers to invent innovative ways to unravel their function and relevance. (frontiersin.org)
  • Genetic
  • Putative targets found by predictive, comparative or genetic methods need validation and consequently a whole area of helminth functional genomics have emerged, with RNAi-mediated gene silencing as the main tool but also attempting to develop stable transgenesis methods. (frontiersin.org)
  • We propose this Research Topic as an open forum to present and discuss these innovative genetic and genomic-based approaches that are helping to develop tools and leading the community towards solutions to understand many of the challenging aspects of helminth biology. (frontiersin.org)
  • MicroRNAs
  • Animal microRNAs (miRNAs) are gene regulatory factors that prevent the expression of specific messenger RNA targets by binding to their 3' untranslated region. (nih.gov)
  • microRNAs (miRNAs), a class of short, non-coding RNA can be found in a highly stable, cell-free form in mammalian body fluids. (biomedcentral.com)
  • RNAi
  • Integrated omics approaches to comprehensively study helminths at a molecular level: including efforts towards the development of a toolkit to unravel gene function, such as transgenesis, RNAi and CrispR-Cas9, and furthering our understanding of transcriptional regulation in helminths with special emphasis in particularities like transplicing, gene editing, post-transcriptional regulation and/or the role of non-coding RNA and epigenetic mechanisms. (frontiersin.org)
  • transgenesis
  • To develop transgenesis as a functional approach for these pathogens, we previously demonstrated that pseudotyped murine leukemia virus (MLV) can transduce schistosomes leading to chromosomal integration of reporter transgenes and short hairpin RNA cassettes. (paperity.org)
  • molecular
  • Ultimately, these advances in molecular and medical helminth biology should one day translate into a new and robust pipeline of drugs, diagnostics, and vaccines for targeting parasitic worms that infect humans. (nih.gov)
  • elegans
  • Here we describe a high-throughput method for reliable identification of polyadenylated RNA termini, and we apply this method, called poly(A)-position profiling by sequencing (3P-Seq), to determine C. elegans 3'UTRs. (nih.gov)
  • elegans and found to contain sequences complementary to a repeated sequence element in the 3' untranslated region (UTR) of lin-14 mRNA, suggesting that lin-4 regulates lin-14 translation via an antisense RNA-RNA interaction. (labome.ru)
  • Humans
  • Small RNA libraries were prepared from total RNA extracted from the nodule fluid of cattle infected with Onchocerca ochengi as well as serum and plasma from humans infected with Onchocerca volvulus in Cameroon and Ghana. (biomedcentral.com)
  • small
  • Our study shows the power of RNA deep sequencing, which can be used to reveal the in vivo expression patterns of small amounts of bacteria in complex intestinal environments. (prolekare.cz)
  • Small non-coding RNAs (sncRNAs) have emerged as important regulators of many processes in animals, from development to immunity. (biomedcentral.com)