• The dissertation thus demonstrates a tight coordination between DEAD box RNA helicase and cancer development. (gsu.edu)
  • A Co-Opted DEAD-Box RNA Helicase Enhances Tomb. (prolekarniky.cz)
  • In this paper, we show that an essential translation factor, Ded1p DEAD-box RNA helicase of yeast, directly affects replication of Tomato bushy stunt virus (TBSV). (prolekarniky.cz)
  • Subsite-specific association of DEAD box RNA helicase DDX60 with the development and prognosis of oral squamous cell carcinoma. (nih.gov)
  • Expression of the cyanobacterial DEAD-box RNA helicase, crhR, is regulated in response to conditions, which elicit reduction of the photosynthetic electron transport chain. (macewan.ca)
  • In particular, we show that a DEAD-box RNA helicase, helicase 1 (HEL-1), promotes longevity by specifically activating the DAF-16/forkhead box O (FOXO) transcription factor signaling pathway. (postech.ac.kr)
  • The mutation was found in a DEAD box RNA helicase gene that is identical to the previously identified low expression of osmotically responsive genes 4 (LOS4) locus, which was defined by the los4-1 mutation that reduces cold regulation of CBFs and their target genes and renders Arabidopsis plants chilling sensitive. (edu.sa)
  • DEAD-box RNA helicases. (embl.de)
  • DExD/H-box RNA helicases are motor proteins participating in nearly all aspects of cellular processes, especially in RNA metabolism. (syr.edu)
  • Activities of the DEAD box (Asp-Glu-Ala-Asp) family of proteins- including RNA-dependent ATPase and RNA helicase- function in all organisms to sculpt RNA-RNA duplex and RNA-protein complexes, ensuring that necessary rearrangements are rapidly and properly resolved during genetic information processing. (gsu.edu)
  • Altogether, the two host factors enhance TBSV replication synergistically by interacting with the viral (−)RNA and the replication proteins. (prolekarniky.cz)
  • After translation of their mRNA-sense genomic RNA(s), the viral RNA and the viral replication proteins are recruited to the site of viral replication in membranous compartments. (prolekarniky.cz)
  • For efficient replication, (+)RNA viruses recruit numerous host proteins [1] - [5] . (prolekarniky.cz)
  • Among the identified host proteins are RNA-binding proteins, such as translation factors, ribosomal proteins and RNA-modifying enzymes [1] . (prolekarniky.cz)
  • The co-opted host proteins likely affect several steps in viral RNA replication, including the assembly of the replicase complex and/or viral RNA synthesis. (prolekarniky.cz)
  • The single genomic RNA codes for two replication proteins, p33 and p92 pol , which are sufficient to support TBSV replicon (rep)RNA replication in yeast ( Saccharomyces cerevisiae ) model host [13] , [14] . (prolekarniky.cz)
  • DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular procsses involving RNA binding and alteration of RNA secondary structure. (nih.gov)
  • 2023). Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. (upenn.edu)
  • Long-term memory consolidation: The role of RNA-binding proteins with prion-like domains. (tcd.ie)
  • Wang, X., Jia, H., Jankowsky, E. & Anderson, J.T. Degradation of hypomodified tRNA(iMet) in vivo involves RNA-dependent ATPase activity of the DExH helicase Mtr4p. (nature.com)
  • Identified as a prototypic member of the DEAD box family and documented as an ATPase and RNA helicase, p68 plays essential and diverse functions in the control of gene expression ranging from pre-mRNA/rRNA processing and mRNA decay/stability to transcriptional activation and initiation. (gsu.edu)
  • The data obtained with wt and ATPase deficient Ded1p mutants support the model that Ded1p unwinds local structures at the 3′-end of the TBSV (−)RNA, rendering the RNA compatible for initiation of (+)-strand synthesis. (prolekarniky.cz)
  • eIF4A is a highly conserved RNA-stimulated ATPase and helicase involved in the initiation of mRNA translation. (aber.ac.uk)
  • The N-terminal domain also contains motif III (S-A-T) which was proposed to participate in linking ATPase and helicase activities. (embl.de)
  • A new yeast poly(A) polymerase complex involved in RNA quality control. (nature.com)
  • Here we show, however, that RNA polymerase II (Pol II) inside human nucleoli operates near genes encoding rRNAs to drive their expression. (nature.com)
  • The hRPC62 subunit of human RNA polymerase III displays helicase activity. (mpg.de)
  • In Eukaryotes, tRNAs, 5S RNA and U6 RNA are transcribed by RNA polymerase (Pol) III. (mpg.de)
  • cystein protease, helicase, and RNA polymerase. (cdc.gov)
  • RNA-directed RNA polymerase. (cathdb.info)
  • TATA-box binding protein is not required for RNA Polymerase II transcription in mouse embryonic stem cells. (elifesciences.org)
  • An ATP-dependent 3'-5' DNA helicase which is a component of the core-TFIIH basal transcription factor, involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. (embl.de)
  • 2022). Sexually dimorphic RNA helicases DDX3X and DDX3Y differentially regulate RNA metabolism through phase separation. (upenn.edu)
  • Our findings revise theories of labour division between the major RNA polymerases, and identify nucleolar Pol II as a major factor in protein synthesis and nuclear organization, with potential implications for health and disease. (nature.com)
  • A fragment of the Trypanosoma brucei ZC3H41 protein encompassing the ATP-dependent RNA helicase domain was successfully subcloned for expression in a bacterial system (Escherichia coli). (iucc.ac.il)
  • Izhaki-Tavor, LS & Dessau, M 2020, ' ATP-dependent RNA helicase domain of the ZC3H41 protein from Trypanosoma brucei: Expression, purification and crystallization ', Acta Crystallographica Section F:Structural Biology Communications , vol. 76, pp. 604-608. (iucc.ac.il)
  • RNA-protein multiome approach helps to discover that the posttranscriptional regulation of the translational machinery is crucial for the fidelity of cortical development. (elifesciences.org)
  • Altered Ribostasis: RNA-Protein Granules in Degenerative Disorders. (tcd.ie)
  • The structure visualizes a transition-state mimic of the reaction and suggests how eukaryotic DEAH/RHA helicases couple ATP hydrolysis to RNA translocation. (cipsm.de)
  • All eukaryotic plus-stranded (+)RNA viruses have similar replication cycles in infected cells. (prolekarniky.cz)
  • Eukaryotic DNA repair helicase RAD3/ERCC-2, an ATP-dependent 5'-3' DNA helicase involved in nucleotide excision repair of UV-damaged DNA. (embl.de)
  • Eukaryotic TFIIH basal transcription factor complex helicase XPB subunit. (embl.de)
  • Eukaryotic ATP-dependent DNA helicase Q. A DNA helicase that may play a role in the repair of DNA that is damaged by ultraviolet light or other mutagens. (embl.de)
  • Bacterial and eukaryotic antiviral SKI2-like helicase. (embl.de)
  • de la Cruz, J., Kressler, D., Tollervey, D. & Linder, P. Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3′ end formation of 5.8S rRNA in Saccharomyces cerevisiae . (nature.com)
  • Additional putative genotypes have been identifi ed through analysis of an 130-nt fragment of the To explore the genetic diversity of avian hepatitis E virus helicase domain, suggesting that diversity within aHEV is strains, we characterized the near-complete genome of a higher than recognized ( 11 ). (cdc.gov)
  • Sloan, K.E., Bohnsack, M.T., Schneider, C. & Watkins, N.J. The roles of SSU processome components and surveillance factors in the initial processing of human ribosomal RNA. (nature.com)
  • Existing models suggest that RNA polymerases I and III (Pol I and Pol III) are the only enzymes that directly mediate the expression of the ribosomal RNA (rRNA) components of ribosomes. (nature.com)
  • All the catfish RNA helicases contained conserved helicase signature motifs, demonstrating that the RNA helicase gene family was highly conserved. (syr.edu)
  • This gene encodes a DEXD/H box RNA helicase that functions as an antiviral factor and promotes RIG-I-like receptor-mediated signaling. (nih.gov)
  • Glomerular endothelial expression of type I IFN-stimulated gene, DExD/H-Box helicase 60 via toll-like receptor 3 signaling: possible involvement in the pathogenesis of lupus nephritis. (nih.gov)
  • Figure 2: Mtr4p unwinding rates versus tether tension and nucleotide composition for unwinding the 16-bp RNA hairpin. (nature.com)
  • Catalyzes RNA-template-directed extension of the 3'-end of an RNA strand by one nucleotide at a time. (cathdb.info)
  • In vitro, wild type recombinant eIF4A1 and its phospho-null variant both support translation in cell free wheat germ extracts dependent upon eIF4A, but the phosphomimetic variant does not support translation and was also deficient in ATP hydrolysis and helicase activity. (aber.ac.uk)
  • DNA helicases utilize the energy from ATP hydrolysis to unwind double-stranded DNA. (cathdb.info)
  • In this study, a total of 54 DExD/H-box RNA helicase genes including 37 DDX (DEAD-box) and 17 DHX (DEAH-box) genes were characterized in channel catfish (Ictalurus punctatus), and annotated through phylogenetic and syntenic analyses. (syr.edu)
  • DEAH-box RNA helicases. (embl.de)
  • RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. (nature.com)
  • Callahan, K.P. & Butler, J.S. TRAMP complex enhances RNA degradation by the nuclear exosome component Rrp6. (nature.com)
  • Mtr4 is an essential RNA helicase associated with the nuclear exosome and aids in the processing and degradation of many RNAs. (richmond.edu)
  • To investigate the ability of the conditional mtr4 mutants in processing various RNAs, we used chemiluminescent northern blotting to demonstrate one conditional mutant's defects in RNA processing and degradation. (richmond.edu)
  • To understand how the 3' to 5' Mtr4 helicase engages RNA and the nuclear exosome, we reconstituted 14-subunit Mtr4-containing RNA exosomes from Saccharomyces cerevisiae, Schizosaccharomyces pombe, and human and show that they unwind structured substrates to promote degradation. (rcsb.org)
  • Competition for the exosome core may ensure that RNA is committed to degradation by DIS3 when engaged by MTR4. (rcsb.org)
  • RNA helicases are a large family of enzymes that regulate the biogenesis and homeostasis of RNA. (postech.ac.kr)
  • Here, we report that a large fraction of RNA helicases regulate the lifespan of Caenorhabditis elegans. (postech.ac.kr)
  • MicroRNAs (miRNAs) and other classes of short non-coding RNAs regulate essential processes in the development and function of the nervous system. (harvard.edu)
  • Bernstein, J., Patterson, D.N., Wilson, G.M. & Toth, E.A. Characterization of the essential activities of Saccharomyces cerevisiae Mtr4p, a 3′>5′ helicase partner of the nuclear exosome. (nature.com)
  • We identified a stable MLE core comprising the DExH helicase module and two auxiliary domains: a dsRBD and an OB-like fold. (cipsm.de)
  • MLE core is an unusual DExH helicase that can unwind blunt-ended RNA duplexes and has specificity for uridine nucleotides. (cipsm.de)
  • The OB-like and dsRBD folds bind the DExH module and contribute to form the entrance of the helicase channel. (cipsm.de)
  • van Hoof, A., Lennertz, P. & Parker, R. Yeast exosome mutants accumulate 3′-extended polyadenylated forms of U4 small nuclear RNA and small nucleolar RNAs. (nature.com)
  • The shield prevents Pol I from producing sense intergenic noncoding RNAs (sincRNAs) that can disrupt nucleolar organization and rRNA expression. (nature.com)
  • We reveal a nucleolar Pol-II-dependent mechanism that drives ribosome biogenesis, identify disease-associated disruption of nucleoli by noncoding RNAs, and establish locus-targeted R-loop modulation. (nature.com)
  • Traditionally, the nucleolar Pol I and nucleoplasmic Pol III are viewed as the sole mammalian RNA polymerases that directly mediate housekeeping ribosome biogenesis. (nature.com)
  • We loaded a human exosome with an optimized DNA-RNA chimera that stalls MTR4 during unwinding and determined its structure to an overall resolution of 3.45 Å by cryoelectron microscopy (cryo-EM). (rcsb.org)
  • EXOSC10 remains bound to the core, but its catalytic module and cofactor C1D are displaced by RNA-engaged MTR4. (rcsb.org)
  • 2023). Genome-wide RNA binding analysis of C9orf72 poly(PR) dipeptides. (upenn.edu)
  • RNA genome. (cdc.gov)
  • LOCUS AB187514 7746 bp RNA linear VRL 01-SEP-2005 DEFINITION Norovirus Hu/GI/Otofuke/1979/JP genomic RNA, complete genome. (cdc.gov)
  • Inclusion of Mtr4p within the TRAMP complex increases the rate constant for unwinding initiation but does not change the characteristics of Mtr4p's helicase mechanism. (nature.com)
  • In situ poly(A) hybridization indicates that the export of poly(A) + RNAs is blocked in the cryophyte/los4-2 mutant at warm or high temperatures but not at low temperatures, whereas the los4-1 mutation weakens mRNA export at both low and warm temperatures. (edu.sa)
  • These results demonstrate an important role of the CRYOPHYTE/LOS4 RNA helicase in mRNA export, plant development, and stress responses. (edu.sa)
  • 7746 /organism="Norovirus Hu/GI/Otofuke/1979/JP" /mol_type="genomic RNA" /strain="Otofuke" /db_xref="taxon:290280" /country="Japan" CDS 5. (cdc.gov)
  • These two superfamilies encompass a large number of DNA and RNA helicases from archaea, eubacteria, eukaryotes and viruses that seem to be active as monomers or dimers. (embl.de)
  • Here, single-molecule high-resolution optical trapping measurements reveal that Mtr4p unwinds RNA duplexes by 3′-to-5′ translocation on the loading strand, that strand separation occurs in discrete steps of 6 base pairs and that a single Mtr4p molecule performs consecutive unwinding steps. (nature.com)
  • Our data indicate that Mtr4p utilizes a previously unknown unwinding mode that combines aspects of canonical translocating helicases and non-canonical duplex-sensing helicases, thereby restricting directional translocation to duplex regions. (nature.com)
  • Analysis of the relative rates of synonymous (dS) and nonsynonymous (dN) substitutions revealed that the RNA helicase genes were subjected to strong negative (purifying) selection. (syr.edu)
  • Meta-analysis was conducted to determine expression of the RNA helicase genes during the critical period (90-110 days post-fertilization, dpf) of male gonad differentiation. (syr.edu)
  • In general, the vast majority of the RNA helicase genes (31) were expressed at higher levels in females than in males. (syr.edu)
  • Cluster identity was assigned based on the top 10 marker genes of each cluster ( Table S2 ), followed by inspection of RNA in situ hybridization patterns. (stowers.org)
  • These findings suggested that RNA helicases may play important roles in sex development and differentiation in teleosts. (syr.edu)
  • DDX60, a DEXD/H box helicase, is a novel antiviral factor promoting RIG-I-like receptor-mediated signaling. (nih.gov)
  • The HCMV helicase-primase complex (pUL105-pUL102-pUL70) is essential for viral DNA replication and could thus be a relevant antiviral target. (frontiersin.org)
  • Bernstein, J. & Toth, E.A. Yeast nuclear RNA processing. (nature.com)
  • Ten of the fifteen mutations cause premature termination of a large DEAxQ-box helicase, the human ortholog of yeast Sen1p, involved in RNA maturation and termination. (nih.gov)
  • Violin plots show distribution of expression levels for ATP-dependent RNA helicase DHX8 (SMED30020946) in cells (dots) of each of the 12 neoblast clusters. (stowers.org)
  • Expression of ATP-dependent RNA helicase DHX8 (SMED30020946) in the t-SNE clustered sub-lethally irradiated X1 and X2 cells. (stowers.org)
  • Anderson, J.T. & Wang, X. Nuclear RNA surveillance: no sign of substrates tailing off. (nature.com)
  • Schmidt, K. & Butler, J.S. Nuclear RNA surveillance: role of TRAMP in controlling exosome specificity. (nature.com)
  • RNAs, including long noncoding RNAs (lncRNA), are known to be abundant and important structural components of the nuclear infrastructure. (harvard.edu)
  • The structure reveals an RNA-engaged helicase atop the non-catalytic core, with RNA captured within the central channel and DIS3 exoribonuclease active site. (rcsb.org)
  • The product Assay kit for Guinea pig Probable ATP-dependent RNA helicase DDX17(DDX17) (ELISA) is intended to be used for research purposes only. (rnagrade.com)
  • The product Assay kit for Guinea pig Probable ATP-dependent RNA helicase DDX17(DDX17) (ELISA) should be kept between two and eight degrees Celsius to ensure the retention of the stability and reactivity of the reagents included in the kit. (rnagrade.com)
  • Promising new inhibitors that target the viral helicase-primase complex have been reported to block replication of herpes simplex and varicella-zoster viruses, but they have no activity against human cytomegalovirus (HCMV), another herpesvirus. (frontiersin.org)
  • However, the role of RNA homeostasis in aging processes remains unknown. (postech.ac.kr)
  • We also find that Ded1p is a component of the tombusvirus replicase complex and Ded1p binds to the 3′-end of the viral minus-stranded RNA. (prolekarniky.cz)
  • In addition, we have developed an in vitro assay for Flock house virus (FHV), a small RNA virus of insects, that also demonstrated positive effect on FHV replicase activity by the added Ded1p helicase. (prolekarniky.cz)
  • After the assembly of the membrane-bound viral replicase complexes (VRC), the viral replicase uses the viral RNA as a template to produce complementary (−)RNA. (prolekarniky.cz)
  • Because HEL-1 and IIS are evolutionarily well conserved, a similar mechanism for longevity regulation via an RNA helicase-dependent regulation of FOXO signaling may operate in mammals, including humans. (postech.ac.kr)
  • RNA helicase activity of Mtr4p is critical for biological functions of the enzyme, but the molecular basis for RNA unwinding is not understood. (nature.com)
  • It has a DNA unwinding activity characteristic of helicases with a 3' to 5' polarity. (embl.de)
  • It acts by opening DNA either around the RNA transcription start site or the DNA. (embl.de)
  • Bacterial ATP-dependent DNA helicase recG. (embl.de)