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  • tracrRNA
  • We show here that in a subset of these systems, the mature crRNA that is base-paired to trans-activating crRNA (tracrRNA) forms a two-RNA structure that directs the CRISPR-associated protein Cas9 to introduce double-stranded (ds) breaks in target DNA. (sciencemag.org)
  • wherein
  • 2. The method according to claim 1 wherein the target gene specific silencing activity is a transcriptional gene silencing (TGS) activity or a post-transcriptional gene silencing (PTGS) activity preferably selected from RNA interference and/or translational attenuation. (freepatentsonline.com)
  • 7. The method according to any of the claims 1- 6 wherein said double stranded RNA molecule is a siRNA or a miRNA. (freepatentsonline.com)
  • 9. The method according to any one of claims 1- 8 wherein the double stranded RNA molecule comprises a double stranded portion and at least one 3′-overhang of from 1-10, preferably from 1-5 nucleotides. (freepatentsonline.com)
  • 12. The method according to any one of claims 1 to 11 wherein the double stranded RNA molecule further comprises 5′- and/or 3′-modifications preferably selected from lipid groups, e.g. cholesterol groups and vitamins. (freepatentsonline.com)
  • 14. The method of any of claims 1 to 13, wherein the antisense strand is completely complementary to said target RNA, wherein complementarity comprises Watson-Crick and base pairs and Wobble (G-U, U-G) base pairs. (freepatentsonline.com)
  • recognition
  • In DNA-targeting CRISPR-Cas systems, the RNA component of the complex encodes target recognition by forming a site-specific hybrid (R-loop) with its complement (protospacer) on an invading DNA while displacing the noncomplementary strand. (pnas.org)
  • vitro
  • In vitro transcribed-CRISPR guide RNAs trigger innate immune response in cells, but can be prevented by removing the triphosphate moiety. (eurekalert.org)
  • Together with novel data from our labs, we find that the optimal on-target efficiency prediction model strongly depends on whether the guide RNA is expressed from a U6 promoter or transcribed in vitro. (biomedcentral.com)
  • programmable
  • Collectively, our data show for the first time that RNA-guided cytidine deaminases are capable of programmable single and multiplex base editing in an invertebrate model. (g3journal.org)
  • complex
  • The present invention relates to methods and compositions for modulating RNA silencing efficiency by providing selective RISC (RNA-induced silencing complex) formation. (freepatentsonline.com)
  • This study provides unprecedented insight into the organization and function of pseudouridylation guide RNPs, and Dr. Terns indicates that "this is only the beginning of the gold mine of new information that this system will yield about a complex that is also involved in rRNA processing and the function of telomerase in eukaryotes. (brightsurf.com)
  • system
  • Nonetheless, to date the CRISPR RNA/Cas9 system has not been adapted for Drosophila , a key genetic model, and germline transmission of Cas9-induced changes has not been achieved in any organism. (genetics.org)
  • single
  • The activities of poor or unsuccessful single-guide RNAs (sgRNAs) initiating with a 5' adenine were improved by rescuing 5' end homogeneity of the sgRNA. (nih.gov)