• Treatment with the Ltc 1 peptide reduced the viral RNA in a dose-dependent manner with EC 50 values of 8.3 ± 1.2, 7.6 ± 2.7 and 6.8 ± 2.5 μM at 24, 48 and 72 h, respectively. (biomedcentral.com)
  • It is for quick and efficient isolation of total RNA from a mixture of RNA, DNA and proteins from samples taken from, animal, plant, yeast, or bacterial cells and will also isolate viral RNA. (severnbiotech.com)
  • Whether it is for protein electrophoresis or western blot, our pre-stained protein markers help you quickly determine the molecular weight of the target protein or evaluate the transfer efficiency. (acrobiosystems.com)
  • The side chains of the lysine residues have been modified by appending the CD40L-derived sequence 143Lys-Gly-Tyr-Tyr146 via a 6-aminohexanoic acid residue as a spacer. (cnrs.fr)
  • Western blotting and qRT-PCR were used to verify the expression of crucial target genes at the protein and RNA levels, respectively. (hindawi.com)
  • In RNA interference experiments, P3H2 protein synthesis was suppressed coordinately with prolyl 3-hydroxylation at Pro- 944, Pro-707, and the C-terminal GPP repeat of the pN 1(II) chain, but Pro-986 remained fully hydroxylated. (documents.pub)
  • Transmission electron microscopy, histological and immunohistochemical techniques, Western blotting and recombinant adeno-associated virus 9 transfection were used to illustrate the effects of CDDPs on IR and autophagy. (bvsalud.org)
  • The short-term neurobehavior tests, magnetic resonance imaging (MRI), laser speckle contrast imaging (LSCI), Evans blue extravasation assay, Fluoro-Jade C (FJC), TUNEL, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), western blotting, and quantitative-PCR were performed in this study. (biomedcentral.com)
  • Besides their canonical roles in translation, tRNAs also originate tRNA-derived small RNAs (tsRNAs), a class of small non-coding RNAs with regulatory functions ranging from translation regulation to gene expression control and cellular stress response. (mdpi.com)
  • Semi-solid (gels) or solid formulations (freeze-dried tablets with controlled release) were also developed. (europa.eu)
  • The aggregation of CP initiated by RNA recognition plays an important role in viral assembly, initiation and elongation. (oncotarget.com)
  • The differential accumulation of JaVH with distinct 3′ UTR suggested that the 3′ UTR of JaVH plays a crucial role in viral RNA accumulation. (biomedcentral.com)
  • Examples include mutations in the non-structural protein genes that increase or decrease subgenomic RNA transcription or alter genomic RNA replication, resulting in modified NOI expression. (allindianpatents.com)
  • Using the OmpR protein, we identify residues in the flexible linker and the C-terminal β-hairpin that enable associations of this archetypical response regulator with thioredoxin, but are dispensable for interactions of this transcription factor to its cognate sensor kinase EnvZ, DNA or RNA polymerase. (nature.com)
  • RNA polymerase transcribes genes encoding downstream adaptive outputs. (nature.com)
  • Japanese encephalitis virus (JEV) NS5 is a viral nonstructural protein that carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) domains. (biomedcentral.com)
  • Analysis of the amino acid sequence of NS5 led to the prediction that it carries both methyltransferase and RNA-dependent RNA polymerase (RdRp) activities. (biomedcentral.com)
  • The CP is essential in maintaining and protecting the RNA genome [ 8 ]. (oncotarget.com)
  • JEV is an enveloped, positive-stranded RNA virus whose genome consists of a single-stranded RNA molecule of approximately 11 kb. (biomedcentral.com)
  • The RNA genome of JEV consists of 98-nucleotide (nt) long 5' untranslated region (UTR) with the type-1 cap structure at its 5' terminus, a single open reading frame (ORF), and a 585-nt long 3'-UTR with no poly(A) tail at its 3' terminus [ 4 ]. (biomedcentral.com)
  • The PAI-vitronectin complex binds to the Arg-Gly-Asp motif of αv integrins and inhibits the integrin-mediated cell migration [ 28 - 33 ]. (biomedcentral.com)
  • Therefore, we examined comparatively five nonviral, lipid-mediated gene transfer methods and adenoviral based infection, as potential tools for efficient delivery of DNA to rat hepatic stellate cells and their transdifferentiated counterpart, i.e. myofibroblasts. (biomedcentral.com)
  • 20 ug of each lysate was boiled for 10 mins in Inv itrogen sample buffer with antioxidant and resolved using the Invitrogen NuPage method on the 10% Bis Tris gel with MOPS buffer, then transferred to Amersham Hybond C additional membrane. (checkpointinhibitor.com)
  • Western blot analysis was performed on whole cell extracts (30µg lysate) of A-431 (Lane 1), COS-7 (Lane 2), MDCK (Lane 3), C2C12 (Lane 4), PC-3 (Lane 5), L6 (Lane 6), RSC96 (Lane 7), tissue extracts (30µg lysate) of Mouse Lung (Lane 8), Rat Stomach (Lane 9), Mouse Pancreas (Lane 10) and Rat Brain (Lane 11). (thermofisher.com)
  • In vitro transcrip- tion and translation (10) used either T7 or T3 RNA polymer- ase for transcription. (nih.gov)
  • The biochemical properties of JEV NS5 have not been characterized due to the lack of a robust in vitro RdRp assay system, and the molecular mechanisms for the initiation of RNA synthesis by JEV NS5 remain to be elucidated. (biomedcentral.com)
  • RNA isolation, amplification, order inhibitor reverse transcription and quantitative PCR RNA was isolated in the samples making use of the RNeasy Mini kit. (checkpointinhibitor.com)
  • For instance, the p37 of pelargonium line pattern virus (PLPV) exerts its VSR function by sequestering small RNAs (Pérez-Cañamás and Hernández 2015 , 2018 ). (biomedcentral.com)
  • The blots were probed with Anti-beta Actin Mouse Monoclonal Antibody (Product # MA5-15739-1MG, 1:1000. (thermofisher.com)
  • The RNA is present in a number of mouse tissues and in the developing mouse fetus. (nih.gov)
  • Secretion of PAI-1 from glial cultures was detected by ELISA and western blotting analysis. (biomedcentral.com)
  • Transgenic offspring were identified by Southern blot analysis of tail DNA. (medscape.com)
  • Antiviral compounds that target RNA and chemicals that aim at stimulating plant disease resistance have received intensive attention in recent years [ 1 , 2 ]. (oncotarget.com)
  • Using the Agrobacterium co-infiltration transient expression system, the p37 of JaVH was shown to effectively suppress the activity of RNA silencing in Nicotiana benthamiana , which proved its role as a VSR (Zhu et al. (biomedcentral.com)
  • JaVH is a (+)-strand RNA virus that is neither 5′-capped nor 3′-polyadenylated. (biomedcentral.com)
  • Metabolic dependencies & novel genetic codes prevent functional transfer of DNA to other cells. (nih.gov)
  • Our results indicate that FuGENE™6-based methods may be optimized sufficiently to offer a feasible approach for gene transfer into rat hepatic stellate cells. (biomedcentral.com)
  • The data further demonstrate that adenoviral mediated transfer is a promising approach for gene delivery to these hepatic cells. (biomedcentral.com)
  • Detailed protocols for efficient gene transfer to various primary cells and continuous cell lines, irrespective whether these cells are grown as monolayers or in suspension have been established during the last decades. (biomedcentral.com)
  • A 39-kb Not I fragment of the human IGFBP-2 cosmid clone chBP2:4 [ 19 ] was gel-purified and microinjected into the pronucleus of single-cell embryos of FVB/N mice. (medscape.com)
  • After shaving of the injury site, mice were transferred into a stereotaxic frame (Just For Mice Stereotaxic Instrument, Stoelting, Solid wood Dale, Illinois) mounted with an electromagnetic controlled impact device (Impact One Stereotaxic Motorized Impactor, Richmond, Illinois). (wwec2012.net)
  • Taken together, we show that repeat RNA-sequestration of SRSF1 triggers the NXF1-dependent nuclear export of C9ORF72 transcripts retaining expanded hexanucleotide repeats and reveal a novel promising therapeutic target for neuroprotection. (nature.com)
  • However, when dark-cultured spores transferred to continuous light, they started to geminate in 48 hours. (scielo.br)
  • The phosphoryl group is transferred from the histidine kinase to a highly conserved aspartic acid residue in the N-terminal receiver domain of the cognate response regulator 3 (Fig. S1a ). (nature.com)
  • The CP forms aggregate discs that interact with the newly synthesized positive sense TMV RNA to assemble progeny virions. (oncotarget.com)
  • Furthermore, in HUVECs, the RNA sequencing data showed that corylin ameliorated cellular senescence. (researchsquare.com)
  • 5. The method as claimed in claim 4, wherein the linker is Gly-Gly-Ser-Gly or Gly- Ser-Ser-Gly. (sumobrain.com)