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  • Polymerase
  • Eukaryotic 5S rRNA is synthesized by RNA polymerase III, whereas other eukaroytic rRNAs are cleaved from a 45S precursor transcribed by RNA polymerase I. In Xenopus oocytes, it has been shown that fingers 4-7 of the nine-zinc finger transcription factor TFIIIA can bind to the central region of 5S RNA. (wikipedia.org)
  • transcription
  • Binding between 5S rRNA and TFIIIA serves to both repress further transcription of the 5S RNA gene and stabilize the 5S RNA transcript until it is required for ribosome assembly. (wikipedia.org)
  • rRNA
  • La protein interacts with these RNAs (including the 5S rRNA) through their 3' oligo-uridine tract, aiding stability and folding of the RNA. (wikipedia.org)
  • genome
  • The recently described phenomenon of gene silencing termed post-transcriptional gene silencing (PTGS) triggered by double-stranded RNA (dsRNA) appears to be a conserved mechanism involved in resistance to viruses, protection of the genome from repetitive elements, developmental timing, and heterochromatin silencing. (grantome.com)
  • The resulting libraries are composed of more than 37 million total genome matched reads mapping to intergenic regions, coding sequences, retrotransposons, inverted, tandem, and other repeated regions of the genome with more than half of the small RNAs arising from intergenic regions. (biomedcentral.com)
  • binding The human genome in particular is known to contain a large amount of SRP RNA related sequence, including Alu repeats. (wikipedia.org)
  • The regions near the 5'- and 3'-ends of the mammalian SRP RNA are similar to the dominant Alu family of middle repetitive sequences of the human genome. (wikipedia.org)
  • (wikipedia.org)
  • For example, the region of the hepatitis C virus genome that encodes the core protein is highly conserved, because it contains an RNA structure involved in an internal ribosome entry site. (wikipedia.org)
  • There are three distinct groups of RNA viruses depending on their genome and mode of replication: Double-stranded RNA viruses (Group III) contain from one to a dozen different RNA molecules, each coding for one or more viral proteins. (wikipedia.org)
  • Negative-sense ssRNA viruses (Group V) must have their genome copied by an RNA replicase to form positive-sense RNA. (wikipedia.org)
  • Retroviruses (Group VI) have a single-stranded RNA genome but, in general, are not considered RNA viruses because they use DNA intermediates to replicate. (wikipedia.org)
  • Studies of the small non-coding RNA transcriptome of this fungus under a variety of different conditions and the subsequent generation of cDNA libraries from size selected small RNA species, identified several non-coding RNAs (ncRNA) within the fungal genome. (wikipedia.org)
  • When the dsRNA is exogenous (coming from infection by a virus with an RNA genome or laboratory manipulations), the RNA is imported directly into the cytoplasm and cleaved to short fragments by Dicer. (wikipedia.org)
  • Biology
  • Presents an overview over the diverse functions RNA plays in fungal biology, highlighting the latest state of knowledge, as well as remaining questions and future challenges in this area. (apsnet.org)
  • Francis Crick recognized the potential significance of the Griffith protein-only hypothesis for scrapie propagation in the second edition of his "Central dogma of molecular biology" (1970): While asserting that the flow of sequence information from protein to protein, or from protein to RNA and DNA was "precluded", he noted that Griffith's hypothesis was a potential contradiction (although it was not so promoted by Griffith). (wikipedia.org)
  • gene expression
  • However, little is known about the spatial distribution of gene expression in fungal development. (biomedcentral.com)
  • Here, we used laser microdissection (LM) and RNA-seq to determine gene expression patterns in young fruiting bodies (protoperithecia) and non-reproductive mycelia of the ascomycete Sordaria macrospora . (biomedcentral.com)
  • Expressing an RNAi construct in maize kernels that targets the gene for alpha-amylase in Aspergillus flavus resulted in suppression of alpha-amylase ( amy1 ) gene expression and decreased fungal growth during in situ infection resulting in decreased aflatoxin production. (springer.com)
  • Here, we demonstrate that maize inbred B104 expressing an RNAi construct targeting the A. flavus alpha-amylase gene amy1 effectively reduces amy1 gene expression resulting in decreased fungal colonization and aflatoxin accumulation in kernels. (springer.com)
  • RNAi
  • RNAi is RNA-dependent gene silencing process that is controlled by the RNA-induced silencing complex (RISC) and is initiated by short double-stranded RNA molecules in a cell's cytoplasm, where they interact with the catalytic RISC component argonaute. (wikipedia.org)
  • double-stranded
  • (beds.ac.uk)
  • It is a double-stranded RNA non-enveloped virus The members of genus Orbivirus within the Reoviridae family are arthropod borne viruses and are responsible for high morbidity and mortality in ruminants. (wikipedia.org)
  • R2D2 carries tandem double-stranded RNA-binding domains to recognize the thermodynamically stable terminus of siRNA duplexes, whereas Dicer-2 the other less stable extremity. (wikipedia.org)
  • organism
  • These fungal prions are generally considered benign, and in some cases even confer a selectable advantage to the organism. (wikipedia.org)
  • novel
  • Conclusions: We demonstrate the success of a novel method of incorporating RNA-seq data into GHMM fungal gene prediction. (edu.au)
  • This family was established in 2016 to taxonomically accommodate Sclerotinia sclerotiorum negative-stranded RNA virus 1 (SsNSRV-1), a novel virus discovered in a hypovirulent strain of Sclerotinia sclerotiorum. (wikipedia.org)
  • The ncRNAs identified were further classified as small nucleolar RNAs (snoRNAs), small nuclear RNAs (snRNAs) or novel ncRNAs. (wikipedia.org)
  • purification
  • In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. (omegabiotek.com)
  • EZ-10 Column Animal total RNA Purification Kit provides a simple spin column technique for preparation of high quality, high-purity intact total RNA. (thomassci.com)
  • The EZ-10 Column Total RNA Mini-Prep Kit allows efficient purification of total RNA from various samples, total RNA is easily purified from animal or human cells and tissues using a simple spin format. (thomassci.com)
  • This kit provides a homogenizer column for filtration and homogenization of viscous plant cell lysate by centrifugation in combination with the HiBind RNA spin column for RNA purification. (omegabiotek.com)
  • bacteria
  • citation needed] The 5'-end of the small (4.5S) SRP RNA of many bacteria is cleaved by RNase P.[citation needed] The ends of the Bacillus subtilis SRP RNA are processed by RNase III. (wikipedia.org)