• This kit provides convenient and rapid methods for the isolation of total RNA from a variety of fungal species. (tebu-bio.com)
  • Purified total RNA from 100 mg fungal species was isolated with the E.Z.N.A.® Fungal RNA Kit. (omegabiotek.com)
  • RNA was extracted from fungal species as follows: sample 1 from E. edodes , sample 2 from V. volvacea , sample 3 from F. velutipes , sample 4 from H. erinaceum . (omegabiotek.com)
  • We designed rRNA-targeting probe sets to detect 42 bacterial and fungal genera or species common in cystic fibrosis (CF) sputum and demonstrated the taxon specificity of these probes, as well as a linear response over more than 3 logs of input RNA. (asm.org)
  • Sets of homologous protein sequences are rarely complete with respect to the fungal species of interest and are often small or unreliable, especially when closely related species have not been sequenced or annotated in detail. (edu.au)
  • In this study, a global survey of the host defense against fungal and bacterial infection was performed on the non-model lepidopteran pest species. (biomedcentral.com)
  • This kit is designed for preparation of high quality total RNA from a wide range of fungal species. (thomassci.com)
  • This kit is designed for preparation of high quality total RNA from a wide variety of plant species and tissues types. (thomassci.com)
  • Fungal infections by Rhodotorula species are increasingly reported in the literature and consist of bloodstream infections, especially in patients with central venous catheters (CVC), as well as centr. (bioportfolio.com)
  • Transcript contigs, protein sequences from multiple fungal species, and ab initio gene predictions were included as evidence sources in the Maker gene prediction pipeline, resulting in 6,992 consensus gene models. (biomedsearch.com)
  • Good examples are mitoviruses found in the two fungal species Sclerotinia homoeocarpa and Ophiostoma novo-ulmi. (wikipedia.org)
  • 2005) described that it is possible to extend the natural host range of Cryphonectria parasitica hypovirus 1 (CHV1) to several fungal species that are closely related to Cryphonectria parasitica using in vitro virus transfection techniques. (wikipedia.org)
  • This species is part of the community of fungal successors of decaying wood. (wikipedia.org)
  • Factors that likely contribute to the under-representation of fungal species among fossils include the nature of fungal fruiting bodies, which are soft, fleshy, and easily degradable tissues and the microscopic dimensions of most fungal structures, which therefore are not readily evident. (wikipedia.org)
  • Phylogenetic analyses suggest that the flagellum was lost early in the evolutionary history of the fungi, and consequently, the majority of fungal species lack a flagellum. (wikipedia.org)
  • Killer toxins are polypeptides secreted by some fungal species that kill sensitive cells of the same or related species, often functioning by creating pores in target cell membranes. (wikipedia.org)
  • However, DNA sequencing revealed a 5-base pair change in the internal transcribed spacer regions (a segment of RNA often used in molecular phylogenetics to identify or distinguish fungal species) between P. cyanescens and the Seattle collections. (wikipedia.org)
  • Studies of the small non-coding RNA transcriptome of this fungus under a variety of different conditions and the subsequent generation of cDNA libraries from size selected small RNA species, identified several non-coding RNAs (ncRNA) within the fungal genome. (wikipedia.org)
  • Viruses in this family are primarily soil-borne, some transmitted by fungal species of the order Chytridiales, others by no known vector. (wikipedia.org)
  • The species description was based on the phylogenetic comparison of the transcribed spacer (ITS) and RNA polymerase II subunit B (RPB2) using maximum likelihood method and by applying the Hasegawa-Kishino-Yano model based on a comparison of 1,062 nucleotides. (wikipedia.org)
  • Molecular analysis of 16S ribosomal RNA gene sequences shows the species to be closely related to Paenibacillus amylolyticus, P. pabuli and P. xylanilyticus. (wikipedia.org)
  • In addition, fungal species vary in intra-specific sequence similarity of the ITS region. (wikipedia.org)
  • The RNA could be modified to maintain its effectiveness as target species evolve tolerance to the original. (wikipedia.org)
  • Other unassessed risks include ecological (given the need for sustained presence for herbicide and other applications) and the possible for RNA drift across species boundaries. (wikipedia.org)
  • Computational methods predict a HAC-like intron with its characteristic RNA structure in 128 out of 156 species studied. (wikipedia.org)
  • Since URA3 allows for both positive and negative selection, it has been developed as a genetic marker for DNA transformations and other genetic techniques in bacteria and many fungal species. (wikipedia.org)
  • C. albicans is the most common fungal species isolated from biofilms either formed on (permanent) implanted medical devices or on human tissue. (wikipedia.org)
  • By the end of the 20th century three more fungal species had been identified as causes of sooty blotch on North Carolina apples, still based on their morphological type: Peltaster fructicola, Geastrumia polystigmatis and Leptodontium elatius. (wikipedia.org)
  • Their 1 month old DNA was extracted and two regions, ITS1 and 28S ribosomal RNA sequenced.Parsimony analysis, bootstrapping and the minimum evolution principle led to groups of species, further described by conidial and colony morphology. (wikipedia.org)
  • The lolines are insecticidal and insect-deterrent compounds that are produced in grasses infected by endophytic fungal symbionts of the genus Epichloë (anamorphic species: Neotyphodium). (wikipedia.org)
  • Double-stranded RNAs (dsRNAs) are widespread in plant pathogenic fungi, but their functions in fungal hosts remain mostly unclear, with a few exceptions. (apsnet.org)
  • Culture-based analyses correlated qualitatively with relative abundance data on bacterial and fungal taxa obtained by NanoString, and the analysis of serial samples demonstrated the use of this method to simultaneously detect bacteria and fungi and to detect microbes at low abundance without an amplification step. (asm.org)
  • IMPORTANCE Here we demonstrate the use of an RNA-based analysis of specific taxa of interest, including bacteria and fungi, within microbial communities. (asm.org)
  • Total RNA can be purified from fresh or frozen filamentous fungi samples using this kit. (thomassci.com)
  • In filamentous fungi, gene silencing through RNA interference (RNAi) shapes many biological processes, including pathogenicity. (figshare.com)
  • The soil is a heterogeneous environment that is densely populated with bacteria and fungi, and as such, represents an outstanding system in which to study the effects of bacterial-fungal interactions. (elifesciences.org)
  • They may have common ancestry with plant positive strand RNA viruses in supergroup 1 with potyvirus lineages, respectively A new clade (as yet unnamed) of six viruses infecting filamentous fungi has been reported. (wikipedia.org)
  • The ITS region is the most widely sequenced DNA region in molecular ecology of fungi and has been recommended as the universal fungal barcode sequence. (wikipedia.org)
  • Many double stranded RNA elements that have been described in fungi do not fit this description, and in these cases they are referred to as virus like particles or VLPs. (wikipedia.org)
  • There are also viruses that simply use fungi as vectors and are distinct from mycoviruses, because they cannot reproduce in the fungal cytoplasm. (wikipedia.org)
  • A protein as a stand-alone infectious agent stands in contrast to all other known infectious agents such as viruses, bacteria, fungi, and parasites, all of which contain nucleic acids (DNA, RNA, or both). (wikipedia.org)
  • Fungal fossils are difficult to distinguish from those of other microbes, and are most easily identified when they resemble extant fungi. (wikipedia.org)
  • Fungal effectors are secreted by pathogenic or beneficial fungi into and around host cells by invasive hyphae to disable defense components or facilitate colonization. (wikipedia.org)
  • 2005). "Fungal biodiversity: Distribution, conservation and prospecting of fungi from India" (PDF). (wikipedia.org)
  • SMN is evolutionarily conserved including the Fungi kingdom, though only fungal organisms with a great number of introns have the Smn gene (or the splicing factor spf30 paralogue). (wikipedia.org)
  • Here, we demonstrate that maize inbred B104 expressing an RNAi construct targeting the A. flavus alpha-amylase gene amy1 effectively reduces amy1 gene expression resulting in decreased fungal colonization and aflatoxin accumulation in kernels. (springer.com)
  • Analysis of trichothecenes mycotoxins in wheat kernels showed that the relative amount of deoxynivalenol (DON), calculated as [DON] per amount of fungal genomic DNA was reduced in all spikes infected with RNAi mutants, suggesting the possibility that the fungal RNAi pathways affect Fg's DON production. (figshare.com)
  • Together these data show that in F. graminearum, different key components of the RNAi machinery are crucial in different steps of fungal development and pathogenicity. (figshare.com)
  • RNAi is RNA-dependent gene silencing process that is controlled by the RNA-induced silencing complex (RISC) and is initiated by short double-stranded RNA molecules in a cell's cytoplasm, where they interact with the catalytic RISC component argonaute. (wikipedia.org)
  • RNAi mixed with a silicone surfactant that let the RNA molecules enter air-exchange holes in the plant's surface that disrupted the gene for tolerance, affecting it long enough to let the herbicide work. (wikipedia.org)
  • For example, the RNA interference (RNAi) mechanism of small interfering RNAs derived from L1 sequences can cause suppression of L1 retrotransposition. (wikipedia.org)
  • Historically, RNA interference was known by other names, including co-suppression, post-transcriptional gene silencing (PTGS), and quelling. (wikipedia.org)
  • Andrew Fire and Craig C. Mello shared the 2006 Nobel Prize in Physiology or Medicine for their work on RNA interference in the nematode worm Caenorhabditis elegans, which they published in 1998. (wikipedia.org)
  • Two types of small ribonucleic acid (RNA) molecules - microRNA (miRNA) and small interfering RNA (siRNA) - are central to RNA interference. (wikipedia.org)
  • RNA interference has an important role in defending cells against parasitic nucleotide sequences - viruses and transposons. (wikipedia.org)
  • RNA interference is under study for possible use as a spray-on insecticide by multiple companies, including Monsanto, Syngenta, and Bayer. (wikipedia.org)
  • Although introns are sometimes called intervening sequences, the term "intervening sequence" can refer to any of several families of internal nucleic acid sequences that are not present in the final gene product, including inteins, untranslated sequences (UTR), and nucleotides removed by RNA editing, in addition to introns. (wikipedia.org)
  • Our approach capitalises on the high quality of fungal transcript assemblies by incorporating predictions made directly from transcript sequences. (edu.au)
  • The RNA appeared to be an autonomous element, since P. infestans DNA did not contain cross-hybridizing sequences. (semanticscholar.org)
  • In addition to the universal ITS1+ITS4 primers used by many labs, several taxon-specific primers have been described that allow selective amplification of fungal sequences (e.g., see Gardes & Bruns 1993 paper describing amplification of basidiomycete ITS sequences from mycorrhiza samples). (wikipedia.org)
  • Sequences that are joined together in the final mature RNA after RNA splicing are exons. (wikipedia.org)
  • Examples of highly conserved sequences include the RNA components of ribosomes present in all domains of life, the homeobox sequences widespread amongst Eukaryotes, and the tmRNA in Bacteria. (wikipedia.org)
  • Statistical models such as profile-HMMs, and RNA covariance models which also incorporate structural information, can be helpful when searching for more distantly related sequences. (wikipedia.org)
  • In combination with HiBind ® RNA spin columns, this method permits purification of high-quality RNA from as much as 200 mg tissue. (tebu-bio.com)
  • The system is also efficient enough to purify total RNA from as little as 10 mg of tissue or 100 cells. (tebu-bio.com)
  • This kit uses a reagent similar to TRIzol for rapid RNA isolation from animal tissue, cells, and some of the plant tissue. (thomassci.com)
  • Of the seven phytotoxins isolated in fungal leaf spots from sugar beet (Beta vulgaris), it showed 89% growth inhibition. (wikipedia.org)
  • Of the seven phytotoxins isolated in fungal leaf spots from sugar beet (Beta vulgaris), betaenone B showed the least amount of phytotoxicity showing only 8% inhibition of growth while betaenone A and C showed 73% and 89% growth inhibition, respectively. (wikipedia.org)
  • DCLs undergo RNase III-type activities to process complementary double-strand RNAs into small RNAs with 21-26 nucleotides in length ( Carmell and Hannon, 2004 ). (frontiersin.org)
  • These fungal prions are generally considered benign, and in some cases even confer a selectable advantage to the organism. (wikipedia.org)
  • Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity towards CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails. (waw.pl)
  • Owing to its specificity for guanine, RNase T1 is often used to digest denatured RNA prior to sequencing. (wikipedia.org)
  • Other ribotoxins also cleave the RNA of the ribosomes, however there are many more points of cleavage- indicating much less specificity. (wikipedia.org)
  • Identification of another antifungal peptide named Drosomycin and RNA blots demonstrated that two distinct pathways(Toll, Imd) exist, involving Drosomycin and Diptericin respectively. (wikipedia.org)
  • Similarities of structure and function between several members in the Drosophila embryo and members in mammals being noted, study "The Dorsoventral Regulatory Gene Cassette spảtzle/Toll/cactus Controls the Potent Antifungal Response in Drosophila Adults" by Lemaitre and Hoffmann in 1996 illuminated the possible existing innate immunity in Drosophila in response to fungal challenge. (wikipedia.org)