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  • antisense
  • To construct transformation vectors that produce RNAs capable of duplex formation, gene-specific sequences in the sense and antisense orientations were linked and placed under the control of a strong viral promoter. (pnas.org)
  • genome
  • While genes comprise less than 2% of our DNA, up to 80% of the genome is copied into RNA molecules that can execute the instructions stored in DNA. (uiuc.edu)
  • sequences
  • Inhibition is sequence-specific in that the nucleotide sequences of the duplex region of the RNA and of a portion of the target gene are identical. (google.com)
  • Included in these studies is identifying those intramolecular and intermolecular sequences in 1/2-sbsRNAs and mRNAs that bind STAU, defining STAU-containing RNA-binding complexes, and characterizing the physiological significance of the various STAU-mediated pathways. (rochester.edu)
  • cells
  • Poliovirus-infected HeLa cells contain a small amount of an RNA that sediments slightly faster than 16S. (sciencemag.org)
  • We show that upon transfection into cells, siRNAs rapidly localize to P-bodies in their native double-stranded conformation, as indicated by fluorescence resonance energy transfer imaging and that Ago2 is at least in part responsible for this siRNA localization pattern, indicating RISC involvement. (pubmedcentralcanada.ca)
  • target
  • A process is provided of introducing an RNA into a living cell to inhibit gene expression of a target gene in that cell. (google.com)
  • c) introducing the RNA into the target cell, thereby inhibiting expression of the target gene. (google.com)
  • 15. The method of claim 12 in which said double-stranded ribonucleic acid structure is at least 25 bases in length and each of the ribonucleic acid strands is able to specifically hybridize to a deoxyribonucleic acid strand of the target gene over the at least 25 bases. (google.com)
  • chromatography
  • After digestion with DNase I, RNA was purified by using chromatography through a P-6 spin column (Bio-Rad) and analyzed by electrophoresis on a sequencing gel. (pnas.org)
  • polyadenylation
  • Over the last 10 years, the study of RNA metabolism in trypanosomatid protozoa has unraveled novel mechanisms of eukaryotic gene expression such as polycistronic transcription ( 1 ), trans-splicing ( 2 , 3 ), mitochondrial RNA editing ( 4 - 6 ), and coupling of trans-splicing and polyadenylation ( 7 ). (pnas.org)
  • Thus, STAU1 binding to 3'UTR inverted Alu elements, like removal of these elements by alternative RNA 3'-end cleavage and polyadenylation, obviates a PKR-mediated innate immune response to cytoplasmic 3'UTR inverted Alu elements. (rochester.edu)
  • recognition
  • Thus, there is great potential in developing chemical probes and therapeutic ligands for the recognition of RNA sequence and structure. (jove.com)
  • infection
  • 14. The method of claim 13 in which a formulation comprised of the RNA is applied on or adjacent to a plant, and disease associated with nematode infection of the plant is thereby reduced. (google.com)
  • Double-stranded RNA pattern and partial sequence data indicate plant virus infection associated with the ringspot disease of European mountain ash ( Sorbus aucuparia L. (springer.com)
  • probes
  • Positive clones were further analysed by using them as hybridisation probes in Northern blots of total plant RNA and in Southern hybridisation with genomic DNA from Sorbus aucuparia leaves. (springer.com)
  • Thus
  • thus, RNA expression from these constructs in transgenic plants can be persistent and heritable. (pnas.org)
  • thereby
  • We found that LINP1 enhances repair of DNA double-strand breaks by serving as a scaffold linking Ku80 and DNA-PKcs, thereby coordinating the NHEJ pathway. (nih.gov)
  • complex
  • Cherry chlorotic rusty spot and Amasya cherry diseases are associated with a complex pattern of mycoviral-like double-stranded RNAs. (deepdyve.com)
  • Snapshots of TRBP-RBD2 in complex with DNA-RNA and dsDNA, captured at the end of 100 ns long simulations, are shown in Fig.1(b) and (c), respectively. (uiuc.edu)