AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPsychiatry and PsychologyPhenomena and ProcessesInformation ScienceHealth Care
RNABase SequenceRNA, MessengerRNA, ViralNucleic Acid HybridizationRNA, Small InterferingRNA, RibosomalNucleic Acid ConformationDNARNA, BacterialRNA EditingRNA SplicingRNA-Binding ProteinsMolecular Sequence DataRibonucleoproteinsDNA-Directed RNA PolymerasesRNA VirusesRNA InterferenceBinding SitesModels, MolecularRNA, Double-StrandedRNA, TransferProtein BindingEscherichia coliRNA, CatalyticOligoribonucleotidesAmino Acid SequenceRNA FoldingRNA Polymerase IIRNA, FungalRNA, Transfer, ValGene Transfer TechniquesRNA StabilityBase PairingRNA, AntisenseRNA HelicasesRNA Processing, Post-TranscriptionalRibosomal ProteinsRNA, Small NuclearRNA PrecursorsRNA, UntranslatedKineticsMacromolecular SubstancesRNA CapsSequence Analysis, RNARibosomesTranscription, GeneticGene Transfer, HorizontalRNA, PlantEmbryo TransferNuclear Magnetic Resonance, BiomolecularCrystallography, X-RayRNA, ProtozoanProtein Structure, TertiaryRNA, NeoplasmBacterial ProteinsRNA Ligase (ATP)ValineProtein ConformationDEAD-box RNA HelicasesRNA Polymerase IIIProtein Structure, SecondaryMolecular WeightTranscription FactorsMutationRNA Polymerase IRNA, NuclearRNA, GuideRNA, Ribosomal, 28STransfer (Psychology)Cell LineRNA, Ribosomal, 18SRNA, Ribosomal, 23SCatechol O-MethyltransferaseFluorescence Resonance Energy TransferPlasmidsRNA TransportRNA, Ribosomal, 16SRNA, Spliced LeaderRNA, SatelliteGenetic VectorsProtein BiosynthesisRNA, ArchaealVirus ReplicationAdoptive TransferHeLa CellsPhospholipid Transfer ProteinsRNA CleavagePatient TransferTemplates, GeneticRNA, Heterogeneous NuclearDNA PrimersRibonucleasesTransfectionGenome, ViralRNA, Small CytoplasmicPromoter Regions, GeneticRNA 3' End ProcessingRNA, Small UntranslatedTransfer Factor
MolecularRibosomeMammalianProteinsClustersTissueInteractionsStructuresClassificationAssayFunctionalAnalyticalInfectionFeaturesComponentsPresentGrowthCellsPatientImportantDrugRequiresViral RNA genomeSequence1994MethodsVariabilityReplicationEnzymesGeneSpeciesComprisesTransporterPreviouslySpecificDataLargeEvidenceRegionCommonResult
Molecular2
  • The molecular mass of SARS-CoV-2 M pro as determined by mass spectroscopy is 33797.0 Da, consistent with its theoretical molecular mass of 33796.8 Da. (nature.com)
  • In particular, the relationship between these dynamic nucleoprotein complexes and the molecular architecture of the ParB protein is unclear and is the subject of the work presented here. (elifesciences.org)
Ribosome2
  • Many cellular processes, including ribosome biogenesis, are regulated through post-transcriptional RNA modifications. (nature.com)
  • We determined subnanometer-resolution cryo-electron microscopy structures of eukaryotic ribosome-Sec61 complexes. (cipsm.de)
Mammalian2
  • While RNA makes up most of the composition of bacterial and cytosolic eukaryotic ribosomes, mammalian mitochondrial ribosomes present a more elaborate protein shell, which aids coping with the oxidative microenvironment. (nature.com)
  • These studies provide important insight into the complex ways in which mammalian cells have evolved to counteract viral infections. (cancer.gov)
Proteins4
  • The genetic information of life on Earth have been conserved in natural nucleotides-A, G, C, T(U) forming two exclusive sets of base pairs A-T(U) & G-C, that complementing to the fundamental processes of DNA replication & RNA transcription by several polymerases and translation to functional proteins via ribosomal reactions. (fortuneonline.org)
  • The UGT1 family constitutes a complex gene locus on human chromosome 2q37 and comprises 13 first exons that encode the unique N-terminal domains of the UGT1A proteins and exons 2 to 5 that encode the C-terminal domain, which is identical in all UGT1A family members ( Owens and Ritter, 1992 ). (aspetjournals.org)
  • The trimeric Sec61/SecY complex is a protein-conducting channel (PCC) for secretory and membrane proteins. (cipsm.de)
  • By bioluminescence resonance energy transfer assay, we show that mutant CALR proteins produced in 1 cell can specifically interact in trans with the TpoR on a target cell. (ox.ac.uk)
Clusters1
  • The use of single-cell RNA sequencing identified 26 clusters that were annotated to 10 different immune cell types. (biomedcentral.com)
Tissue4
  • Many complex disorders are driven by a coupling of housekeeping (universally expressed in all tissues) and tissue-selective (expressed only in specific tissues) dysregulated pathways. (biomedcentral.com)
  • RNA isolation and cDNA synthesis Tissue homogenization from 15 replicates from each group was attained inside a mortar with liquid nitrogen. (alkpathway.com)
  • Quick, tissue was homogenized within a mortar with liquid nitrogen and complete RNA was extracted making use of Trizol reagent and Micro to Midi Kit prior to DNase therapy. (alkpathway.com)
  • Immunohistochemistry Immunohistochemistry was performed using formalin-fixed, paraffin-embedded tissue sections using the avidin-biotin peroxidase complex method. (angiogenesis-blog.com)
Interactions2
  • These interactions, also referred to as the interactome , embody a complex network of functional pathways that closely work together to modulate the cellular machinery. (biomedcentral.com)
  • New insights about the evolution, recognition and structural modularity of GNRA and A-minor RNA-RNA interactions are proposed. (cnrs.fr)
Structures1
  • Expression of this genome entails several, highly regulated processes, with newly synthesised transcripts being cleaved, chemically modified, polyadenylated and further matured in neighbouring structures known as mitochondrial RNA granules (MRGs). (nature.com)
Classification1
  • Taxonomic classification of those E. ictaluri specific phages must rely on a synthesis of morphological and genomic details, taking into consideration that phage evolution continues to be profoundly directed by lateral gene transfer, and that a rational hierarchical technique of phage classification really should be based within the degree of DNA and protein sequence identity for several genetic loci. (hivprotease-signal.com)
Assay1
Functional1
  • Importantly, the CALR-sTFR1 complex remains functional for TpoR activation. (ox.ac.uk)
Analytical2
  • Current techniques for small-molecule detection suffer from various limitations including low analytical sensitivity and complex sample processing. (justia.com)
  • He designs analytical devices and methods for complex data acquisition, simulation and analysis in biopharmaceutical and biomedical applications. (cancer.gov)
Infection2
  • Here, we characterized the staphylococcal transcriptome in vivo and the joint fluid metabolome in a prosthetic joint infection with an acute presentation using deep RNA sequencing and nuclear magnetic resonance spectroscopy, respectively. (biomedcentral.com)
  • The aim of this study was to compare the in vivo expression of virulence and metabolic genes of S. aureus in a prosthetic joint infection in a human subject with growth in vitro as reference using RNA sequencing (RNA-seq). (biomedcentral.com)
Features1
  • As the programs for the simulations such as Amber, CHARMM, BOSS and so on have good parameters for DNA, they succeeded in producing dynamic features of complexes of DNA and fluorescent materials (FMs).However, it is necessary to always make parameters for each FM when new FMs are designed for a specific DNA sequence. (int-conf-chem-structures.org)
Components3
  • The review also outlines the vast applications of UBPs as novel information storage components as well as in creation of semi-synthetic organisms expressing non canonical amino acids, high affinity aptamer generation, PCR based diagnostics and sitespecific labelling of RNAs. (fortuneonline.org)
  • While the RNA components of the mitoribosome are mitochondrially-encoded, all MRPs and assembly factors are encoded in the nuclear genome, thus requiring coordination between two genomes for the assembly of these macromolecular complexes. (nature.com)
  • Amplicon sequencing was used for detection of bacteria in fluid obtained by sonication of prosthesis components (all joint fluid was used for RNA-seq). (biomedcentral.com)
Present1
  • In its absence, mtLSU particles (visualized by cryo-EM at the resolution of 2.6 Å) present disordered RNA domains, partial occupancy of bL36m and bound MALSU1:L0R8F8:mtACP anti-association module, allowing five mtLSU biogenesis intermediates with different intersubunit interface configurations to be placed along the assembly pathway. (nature.com)
Growth1
  • Growth occurred in the presence of methoxylated aromatic compounds (3,4-dimethoxybenzoate and vanillate) together with complex proteinaceous substrates. (uibk.ac.at)
Cells1
  • This is consistent with CALR-mutated cells that expose TpoR carrying immature N-linked sugars at the cell surface. (ox.ac.uk)
Patient1
  • Treatment sis pattern, and the patient was subsequently intubated and plans for the patient included three courses of CHOP (cyclo- transferred to the intensive care unit. (cdc.gov)
Important1
  • γ-Secretase is a pivotal intramembrane-cleaving protease complex and important drug target for Alzheimer's disease. (cipsm.de)
Drug1
  • We identified a mechanism-based inhibitor (N3) by computer-aided drug design, and then determined the crystal structure of M pro of SARS-CoV-2 in complex with this compound. (nature.com)
Requires1
  • The reaction catalyzed by PHM is still not fully understood, but requires two single electron transfer steps. (biomedcentral.com)
Viral RNA genome2
  • Hallmarks of HCV infection are its high propensity in establishing persistence and the fact that the viral RNA genome undergoes extensive mutations during replication. (bmj.com)
  • The nucleoprotein (NP) binds the viral RNA genome as oligomers assembled with the polymerase in a ribonucleoprotein complex required for transcription and replication of influenza A virus. (shengsci.com)
Sequence3
  • Some tRNAs contain a fifth stem-loop named extra arm, which is located in the RNA sequence between the anticodon stem and the T stem [ 5 ]. (biomedcentral.com)
  • Thus each amino acid is held in its proper place while the polymerizing mechanism links it to the two adjacent amino acids ' determined by the sequence of nucleotides in the information-carrying RNA. (nih.gov)
  • The infrared absorption and 1H nuclear magnetic resonance analyses of chloroform solutions of the terminally-blocked segment corresponding to the 2-9 sequence of emerimicins III and IV, -(Aib)3-L-Val-Gly-L-Leu-(Aib)2-, are consistent with the presence of a 3(10)-helical structure of high thermal stability. (shengsci.com)
19942
  • The first example of this was Rhodobacter sphaeroides in 1989, but additional discoveries quickly followed with Brucella melitensis in 1993, Burkholderia cepacia complex in 1994, Rhizobium meliloti in 1995, Bacillus thuringiensis in 1996, and now about 10% of bacterial species are known to have large replicons that are separate from the main chromosome. (wikipedia.org)
  • 28, 1994 and entitled "Multifunctional molecular complexes for gene transfer to cells" which is incorporated herein by reference. (justia.com)
Methods2
  • The results obtained for Hb A or des-His Hb A with two methods were completely mutually consistent. (researchgate.net)
  • The present invention is in the field of methods for the transfer of genetic information, e.g., foreign DNA, into target cells, especially eukaryotic cells. (justia.com)
Variability1
  • Consistent with GWA studies, we found large variability in our datasets. (plos.org)
Replication2
  • HCV replicons were used to test the effect of escape mutations on HCV protease activity and RNA replication. (bmj.com)
  • Introduction of mutations at five positions of the 1073-1081 epitope prevented CTL recognition but three of these reduced protease activity and RNA replication. (bmj.com)
Enzymes1
  • The activities of these enzymes are consistent with degradation of the mucosal layer of the human gastrointestinal tract, glycosaminoglycans and other cellular glycans found throughout the body. (embl-heidelberg.de)
Gene4
  • A seeming paradox was posed by the native-like biochemical properties of inherited Swyer variant Y72F: its near-native gene-regulatory activity is consistent with the father's male development, but at odds with the daughter's XY female somatic phenotype. (nih.gov)
  • To study gene expression during neuronal cell death, we carried out a differential screen of an array of 9,120 cDNA clones from a human infant brain library (library 1NIB [ 20 ]) with complex cDNA targets derived from neuronally differentiated rat pheocytochroma PC12 cells treated with C 2 -ceramide compared to control PC12 cells. (biomedcentral.com)
  • The ultimate usefulness of gene transfer techniques is of enormous potential benefit in a number of areas. (justia.com)
  • The transfer of genetic material into cells is the basis of a number of processes now widely accepted in the areas of molecular biology, gene therapy and genetic immunization. (justia.com)
Species1
  • The formation of fruiting bodies (FBs, basidiomes, basidiocarps) that are in particular formed by species from the Basidiomycota class Agaricomycetes [ 1 ] is one of the most complex developmental processes in the fungal life cycle. (biomedcentral.com)
Comprises1
  • and since the problems are several and different, the present invention comprises a multifunctional complex, i.e., a molecular conjugate of various ligands intended to surmount specific obstacles. (justia.com)
Transporter3
  • However, understanding the underlying molecular mechanism has been hindered by the complete absence of structures for any EIIA(Glc)-transporter complexes. (nih.gov)
  • Here we present the 3.9 Å crystal structure of Escherichia coli EIIA(Glc) in complex with the maltose transporter, an ATP-binding cassette (ABC) transporter. (nih.gov)
  • Consistent over-expression of the two contigs homology to CYP96A13 and ABCC1 transporter, respectively, were further qRT-PCR validated using additional plants from the resistant and susceptible populations. (biomedcentral.com)
Previously2
  • The parameters obtained in the final trial were also consistent with those derived from previously published data on the growth of Pacific angel sharks, despite the differences in some life history traits and highly variable growth rates of this specific population. (elasmollet.org)
  • Furthermore, minor increases in RNA expression were seen in FSHD2 myoblasts for some of the PCDH β cluster isoforms, tRNA isoforms, and a HOXB isoform in comparison to controls, in addition to the previously reported effects on DUX4 expression. (biomedcentral.com)
Specific1
  • The complex is comprised of A) said nucleic acid composition and B) a transfer moiety comprising 1) one or more cationic polyamines bound to said nucleic acid composition, 2) one or more endosome membrane disrupting components attached to at least one nitrogen of the polyamine and 3) one or more receptor specific binding components. (justia.com)
Data3
  • Consistent with the contractile data, phosphorylation of phospholamban at threonine-17 was higher in TG hearts. (frontiersin.org)
  • Emerging studies provide new data shedding some light on the complex and pivotal role of red blood cells (RBCs) in nitric oxide (NO) metabolism and paracrine regulation of endothelial function. (mdpi.com)
  • For instance, you can left 4 dead 2 unlocker download Mac apps that get barely used, remove duplicates and similar files, transfer large old data especially movies and photos to an external backup drive, clean web browser junks, and empty Trash, etc. (yetrecords.com)
Large2
  • It must contain large, complex molecules capable of representing @ large amount of information intisir possible alternative configurations. (nih.gov)
  • There is therefore a requirement for more fundamental insight into this complex pathway that involves a very large number of components. (biomedcentral.com)
Evidence2
  • The elucidation of a codon pattern should provide evidence on the mechaniam of transfer RNA specificity. (nih.gov)
  • Since its discovery, evidence that siRNA was able to act as an RNA interference effector, led to its acceptation as a novel medicine. (mdpi.com)
Region2
Common1
  • Sterling AP, our laboratory has recently shown that E6-AP and RPF-1 synergistically enhance PR transactivation, and that they may exist in a common complex in vivo (McKenna et al. (forex-consultant.com)
Result1
  • Inhibition of ALS enzyme will affect synthesis of the branched-chain amino acids (Val, Leu and Ile) and eventually result in the death of plants. (biomedcentral.com)