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  • exon
  • Paronetto MP, Bernardis I, Volpe E, Bechara E, Sebestyen E, Eyras E, Valcarcel J. Regulation of FAS exon definition and apoptosis by the ewing sarcoma protein. (upf.edu)
  • Depletion and overexpression experiments showed that EWS promotes exon 6 inclusion and consequently the synthesis of the proapoptotic FAS/CD95 isoform, whereas an EWS-FLI1 fusion protein characteristic of Ewing sarcomas shows decreased activity. (upf.edu)
  • Biochemical analyses revealed that EWS binding promotes the recruitment of U1snRNP and U2AF65 to the splice sites flanking exon 6 and therefore exon definition. (upf.edu)
  • expression
  • Consistent with a role for EWS in the regulation of programmed cell death, cells depleted of EWS show decreased sensitivity to FAS-induced apoptosis, and elevated EWS expression enhances apoptosis in EWS-haploinsufficient Ewing sarcoma cells. (upf.edu)
  • localization
  • After export into the cytoplasm, Bach1 forms fiber-like structures on microtubules by colocalizing with intracellular hyaluronic acid-binding protein (IHABP), which regulates the subcellular localization of Bach1 [ 13 ]. (hindawi.com)
  • RBP-RNA interaction can begin during transcription and be sustained until the RNA is degraded or occur transiently to mediate or regulate specific processes, e.g. splicing, transport, localization, translation and decay [ 2 ]. (biochemsoctrans.org)
  • Previously, however, non-phosphorylation PTMs with equally important regulatory functions have emerged, producing a growing library of reversibly and differentially-modified cytoplasmic PTRPs, including RBPs, whose modification state may alter their structure/conformation and thereby regulate their function, e.g. by influencing their cellular localization, RNA-binding activity, protein-protein interactions or combinations thereof [ 5 ]. (biochemsoctrans.org)
  • RNA localization pathways direct numerous mRNAs to distinct subcellular regions and affect many physiological processes. (rupress.org)
  • cytoplasmic
  • Under normal physiological conditions, nuclear Nrf2 contributes to vascular protection by inducing expression of the glutamate cysteine ligase modulatory subunit (GCLM) and the light chain component of system x c − (xCT) in human endothelial cells, while cytoplasmic Nrf2 is bound and inhibited by Kelch-like ECH-associated protein 1 (Keap1) [ 15 ]. (hindawi.com)
  • Instead, we show that translation occurs within cytoplasmic Fus granules leading to local protein production from APC-RNPs. (rupress.org)
  • interactions
  • The pathway maps illustrate protein interactions and regulation to provide a comprehensive picture of signaling and disease processes. (bio-rad.com)
  • stress granules
  • Several RNA granule types exist that share common components and are either constitutively present (such as neuronal transport granules and P-bodies) or form in response to stress (stress granules). (rupress.org)
  • interaction
  • Calmodulin (CaM) is recognized as a major calcium sensor and orchestrator of regulatory events through its interaction with a diverse group of cellular proteins. (embl-heidelberg.de)
  • Many investigations have focused on defining the region of interaction between CaM and its cellular targets and the action of CaM on target protein function. (embl-heidelberg.de)
  • Three recognition motifs for CaM interaction are discussed in the context of experimental investigations of a variety of CaM target proteins. (embl-heidelberg.de)
  • assemblies
  • Our results suggest that at least some of the disorders caused by the aggregation of human prion-like proteins would rely on the formation of classical amyloid assemblies rather than being caused by amorphous aggregates. (biomedcentral.com)
  • Expression
  • The gene and protein expression data of age- and stage-matched prostate cancer specimens from The Cancer Genome Atlas were analyzed. (biomedcentral.com)
  • B) Two representative clones of A673/TR/empty, A673/TR/preLOX, A673/TR/LOX-PP and A673/TR/LOXenz cells were incubated in the absence or in the presence of doxycycline (DOX, 1 µg/ml) during 48 hours to induce the expression of the corresponding proteins. (nih.gov)
  • human
  • Isolation of cDNAs encoding a human protein that binds selectively to DNA modified by the anticancer drug cis-diamminedichloroplatinum(II). (springer.com)
  • Human RNA binding proteins containing intrinsically disordered regions with an amino acid composition resembling those of yeast prion domains, like TDP-43 or FUS, are being found to aggregate in different neurodegenerative disorders. (biomedcentral.com)
  • This observation raises the question of whether it is indeed the lack of an ordered structure in these human prion-like protein aggregates the underlying cause of their toxicity in different pathological states. (biomedcentral.com)
  • Here we characterize the IBs formed by the human prion-like RNA-processing protein HNRPDL. (biomedcentral.com)
  • type
  • Prion proteins are a particularly intriguing type of amyloids, since their aggregated states have a self-perpetuating ability. (biomedcentral.com)
  • motifs
  • Bach1 contains six cysteine-proline (CP) motifs, four of which are located in a heme-binding region near the C-terminus. (hindawi.com)
  • Because CaM can bind with high affinity to a relatively small alpha-helical region of many proteins, success in clearly defining the essential elements of CaM binding motifs seems feasible and should provide a means of identifying CaM binding proteins. (embl-heidelberg.de)
  • representative
  • Swapping experiments in which the TEL oligomerization domain was exchanged by the homologous domains of representative vertebrate ETS proteins including ETS‐1, ERG‐2 and GABPα show that oligomerization is a specific property of the TEL amino‐terminal conserved domain. (embopress.org)