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  • Total RNA
  • total RNA from a Gram negative organism. (bio.net)
  • For microarray analysis and other sensitive applications, Ambion also recommends a DNase treatment (such as with DNA free Treatment and Removal Reagents ) to remove contaminating genomic DNA from the total RNA prior to the MICROB Enrich procedure. (bio-medicine.org)
  • In the first step of the MICROB Enrich procedure, host-bacterial total RNA is incubated with an optimized mixture of capture oligonucleotides that bind to the mammalian 18S and 28S rRNAs and polyadenylated RNAs. (bio-medicine.org)
  • Rat liver total RNA (25 g) and E. coli total RNA (2 g) were mixed together and subjected to the MICROB Enrich procedure. (bio-medicine.org)
  • Another mixed sample of total RNA was subjected to a mock MICROB Enrich procedure in which the capture oligonucleotide mix was left out of the reaction. (bio-medicine.org)
  • Even with a short 6 hour IVT incubation time, 100 ng of input total RNA results in sufficient aRNA for several replicate array experiments. (thermofisher.com)
  • How I can to remove specific sequence from a fungu of a plant-fungu interaction of total RNA-Seq data? (usegalaxy.org)
  • small
  • The lack of an appropriate method for bacterial RNA amplification has hampered studies that rely on rare or limited samples, such as environmental collections, small volume cultures, and bacterial RNA isolated from host cells. (thermofisher.com)
  • There has recently been a surge in studies, including one by Waters et al ( 2017 ) in this issue of The EMBO Journal , that have used clever variations of the RNA ‐seq technique to comprehensively map small RNA -target networks. (embopress.org)
  • Because they are able to accurately make DNA copies of almost any type of RNA from very small amounts of starting material, they would do a better job of catching biomarkers for disease than anything currently available in an RNA-based liquid biopsy. (medicalautomation.org)
  • Altuvia S, Weinstein-Fischer D, Zhang A et al (1997) A small, stable RNA induced by oxidative stress: role as a pleiotropic regulator and antimutator. (springer.com)
  • Barquist L, Vogel J (2015) Accelerating discovery and functional analysis of small RNAs with new technologies. (springer.com)
  • Barquist L, Westermann AJ, Vogel J (2016) Molecular phenotyping of infection-associated small non-coding RNAs. (springer.com)
  • Strong σ70 promoters were predicted upstream of all novel small RNAs, indicating the potential for transcriptional activity. (biomedcentral.com)
  • The small RNA nitrogen stress-induced RNA 1 (NsiR1) is produced by Cyanobacteria under conditions of nitrogen deprivation. (wikipedia.org)
  • 1984 Rajneeshee bioterror attack AsrC small RNA Bacterial small RNA HilD 3'UTR PinT small RNA Typhoid Mary Giannella RA (1996). (wikipedia.org)
  • It was originally identified along with 26 other non-coding RNAs using a tiled Caulobacter microarray protocol specifically aimed at detecting small RNAs. (wikipedia.org)
  • agarose
  • RNA was precipitated and an equal fraction of each sample was analyzed by denaturing agarose gel electrophoresis. (bio-medicine.org)
  • A 1 µg aliquot of DNA from each respective bacterial sample was resolved on a 1% agarose gel in 0.5X TBE at 150 volts for 16 hours using a BioRad CHEF DRII system. (sigmaaldrich.com)
  • complementary
  • TGIRTs find strands of RNA and create complementary strands of DNA that encode the same information and can be rapidly sequenced to provide diagnostic information. (medicalautomation.org)