• The downstream transformation of BA involved a meta -cleavage pathway, although its genetic organization and function was not revealed and the overall network of genes driving the full metabolic pathway of OPP is still not known. (nature.com)
  • 4-OH-E1 meta-cleavage was catalyzed by a dioxygenase encoded by IM701_20340 or IM701_21000 or IM701_09625. (bvsalud.org)
  • This enzyme catalyses the following chemical reaction 2-hydroxymuconate-6-semialdehyde + NAD+ + H2O ⇌ {\displaystyle \rightleftharpoons } (2Z,4E)-2-hydroxyhexa-2,4-dienedioate + NADH + 2 H+ This substrate for this enzyme is formed by meta ring cleavage of catechol (EC 1.13.11.2, catechol 2,3-dioxygenase), and is an intermediate in the bacterial degradation of several aromatic compounds. (wikipedia.org)
  • However, the microbial metabolic pathway of the fungicide and the genetic systems driving its degradation by strain P3 remain unknown. (nature.com)
  • Dienelactone hydrolases play a crucial role in chlorocatechol degradation via the modified ortho cleavage pathway. (inrae.fr)
  • The analysis of both the metagenome of the consortium and the reconstructed metagenome-assembled genomes show that the most abundant bacterial genus in the consortium, Acidocella , possess many of the genes required for the degradation of diesel fuel aromatic hydrocarbons, which are often the most toxic component. (springeropen.com)
  • 2021. Differential roles of three different upper pathway meta ring cleavage product hydrolases in the degradation of dibenzo-p-dioxin and dibenzofuran by Sphingomonas wittichii strain RW1. (nih.gov)
  • The up-regulated members of ABC transport systems, aromatics degradation, and fatty acid degradation indicated a reinforced transfer and utilization of E2. (bvsalud.org)
  • Comparative genome analysis showed that strain MC4 harbors the complete pathway (lin genes) required for HCH degradation. (bvsalud.org)
  • The hydrolysis-, hydroxylated-, monoglucosylated-, methylated products and glutathione (GSH) conjugated derivative were identified and new degradation pathway of TPHP mediated by microorganism was proposed. (bvsalud.org)
  • His primary areas of investigation include Organic chemistry, Toluene dioxygenase, Pseudomonas putida, Biochemistry and NAD+ kinase. (research.com)
  • The concepts of his Pseudomonas putida study are interwoven with issues in Escherichia coli, Electron donor, Dioxygenase, Dehydrogenase and Naphthalene. (research.com)
  • Stereochemistry, Organic chemistry, Biochemistry, Enzyme and Pseudomonas putida are his primary areas of study. (research.com)
  • His Pseudomonas putida research is multidisciplinary, incorporating elements of Nuclear chemistry, Escherichia coli, Toluene, Chromatography and Toluene dioxygenase. (research.com)
  • His Escherichia coli research integrates issues from Regioselectivity, Microorganism, Pseudomonas putida, Metabolite and Toluene dioxygenase. (research.com)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • These results contribute to a better more biomass-derived carbohydrates are also used for produc- understanding of the aromatic metabolism of P. putida in tion of chemicals, a further valorization of lignin is an essential terms of growth and uptake rates, which will be helpful for component. (lu.se)
  • The root-mean-square deviation from ideal bond distances for the model is 0.014 A and the error in atomic co-ordinates is estimated to be 0.15 A. DLH is a monomeric enzyme containing 236 amino acid residues and is a member of the beta-ketoadipate pathway found in bacteria and fungi. (inrae.fr)
  • The second ben/cat operon was located in a 92-kb scaffold along with (i) an operon ( opp ) comprising genes for the transformation of OPP to BA and 2-hydroxypenta-2,4-dienoate (and genes for its transformation) and (ii) an incomplete biphenyl catabolic operon ( bph ). (nature.com)
  • Therefore, elucidation of the microbial metabolic pathway of OPP is a prerequisite for the downstream exploitation of strain P3 in any environmental application. (nature.com)
  • They isolated a Pseudomonas azelaica strain HPB1 which was able to degrade OPP through the production of 2,3-dihydroxybiphenyl. (nature.com)
  • 2021. Separate upper pathway ring cleavage dioxygenases are required for growth of Sphingomonas wittichii strain RW1 on dibenzofuran and dibenzo-p-dioxin. (nih.gov)
  • 2-Hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85, xylG [gene], praB [gene] ) is an enzyme with systematic name (2E,4Z)-2-hydroxy-6-oxohexa-2,4-dienoate:NAD+ oxidoreductase. (wikipedia.org)
  • Dienelactone hydrolase (DLH), an enzyme from the beta-ketoadipate pathway, catalyzes the hydrolysis of dienelactone to maleylacetate. (inrae.fr)
  • David T. Gibson has included themes like Plasmid, DNA, Cleavage and Hydroquinone in his Enzyme study. (research.com)
  • Specific activities of Catechol-2,3-dioxygenase and effects of temperature and pH and their stabilities on the enzyme relative activities were observed. (openmicrobiologyjournal.com)
  • Twelve intermediate products were detected in the water-sediment system, including the observation of the glutathione (GSH) conjugated derivative, monoglucosylated product, (OH)2-DPHP and CH3-O-DPHP for the first time in microorganism-mediated TPHP transformation. (bvsalud.org)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • Cytochrome P450 monooxygenase (EstP1), 2-keto-4-pentenoate hydratase, pyruvate dehydrogenase, acetyl-CoA acetyltransferase, TonB-dependent receptor were involved in E2 catabolism. (bvsalud.org)
  • Genomic analysis identified two orthologous operons encoding the ortho -cleavage of benzoic acid (BA) ( ben/cat ). (nature.com)
  • Metabolism of 4-amino-3-hydroxybenzoic acid by Bordetella sp. (wikipedia.org)
  • He works mostly in the field of Biochemistry, limiting it down to topics relating to Pseudomonas and, in certain cases, Cell-free system, Substituent and Alcohol oxidoreductase, as a part of the same area of interest. (research.com)
  • 6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
  • 2-keto-4-pentenoic acid might be an emblematic product of such process. (bvsalud.org)
  • Its application results in the production of large wastewater volumes which require treatment on site 2 . (nature.com)
  • Our inhibitor binding studies suggest that its substrate, dienelactone, is held in the active site by hydrophobic interactions around the lactone ring and by the ion pairs between its carboxylate and Arg-81 and Arg-206. (inrae.fr)
  • Advanced oxidation processes (APOs) of aromatic compounds, particularly of phenol, yield several benzene derivatives, such as hydroquinone, catechol, and resorcinol, as intermediate metabolites of its transformation. (hindawi.com)
  • The formation of hydroquinone and -benzoquinone at early stages of phenol oxidation increases the toxicity of phenol wastewaters, showing that these compounds were more toxic and less degradable than the original pollutant [ 2 ]. (hindawi.com)
  • are capable of utilizing this aromatic compound [ 10 - 15 ], there are a considerable number of studies about the toxic effects of hydroquinone. (hindawi.com)
  • 2-Hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85, xylG [gene], praB [gene] ) is an enzyme with systematic name (2E,4Z)-2-hydroxy-6-oxohexa-2,4-dienoate:NAD+ oxidoreductase. (wikipedia.org)
  • that degraded 200 mg/L of paracetamol in approximately 10 hours while excreting a dark brown component to the medium, and a slow-growing Pseudomonas sp. (biorxiv.org)