Young KM , Reinhart-King CA , Cellular mechanosignaling for sensing and transducing matrix rigidity Current opinion in cell biology. (vanderbilt.edu)
Integrin-mediated1
Vinculin binding sites are protein domains predominantly found in talin and talin-like molecules, enabling binding of vinculin to talin, stabilising integrin-mediated cell-matrix junctions. (wikipedia.org)
Substrate3
Finally, talin is a substrate for the calcium-ion activated protease, calpain II, which is also concentrated at points of cell-substratum contact. (wikipedia.org)
how a cell can detect, measure and respond to the rigidity of its substrate and how these processes apply to larger biological systems. (mechanobio.info)
Our findings showed that fibroblasts respond to substrate rigidity by recruiting more force-bearing integrins and modulating integrin sampling frequency of the ECM, rather than simply overloading the existing integrin-ligand bonds, to promote focal adhesion maturation. (bvsalud.org)
Extracellular2
It also allows cells to measure extracellular rigidity, since cells in which talin is prevented from forming mechanical linkages can no longer distinguish whether they are on a soft or rigid surface. (wikipedia.org)
Extracellular matrix (ECM) rigidity serves as a crucial mechanical cue impacting diverse biological processes. (bvsalud.org)
Integrins4
In these situations, talin codistributes with concentrations of integrins in the plasma membrane. (wikipedia.org)
Furthermore, in vitro binding studies suggest that integrins bind to talin, although with low affinity. (wikipedia.org)
It binds to ß-catenin/N-cadherin complexes in apical adherens junctions (AJs), which maintain cell-to-cell adhesions, and to talin/integrins in the focal adhesions (FAs) that attach cells to the basal membrane. (bvsalud.org)
Formation of the complex between VBS and vinculin requires prior unfolding of this middle domain: once released from the talin hydrophobic core, the VBS helix is then available to induce the 'bundle conversion' conformational change within the vinculin head domain thereby displacing the intramolecular interaction with the vinculin tail, allowing vinculin to bind actin. (wikipedia.org)
Adhesions1
Discovered in 1983 by Keith Burridge and colleagues, talin is a ubiquitous cytosolic protein that is found in high concentrations in focal adhesions. (wikipedia.org)
Focal adhesion1
Recently Kumar et al combined cellular electron cryo-tomography with FRET based tension measurements and find that the regions of high talin tension within focal adhesion have highly aligned and linear underlying filamentous actin structures while regions of low talin tension have less well-aligned actin filaments. (wikipedia.org)
Defines1
By buffering cellular forces, talin defines the physiological force range in which a number of mechanosensitive cellular processes can take place. (mechanobio.info)
Affecting adhesion1
Talin-1 is involved in each part of extravasation affecting adhesion, trans-endothelial migration and the invasion stages. (wikipedia.org)
Cellular1
However, understanding the molecular mechanisms of rigidity sensing has been limited by the spatial resolution and force sensitivity of current cellular force measurement techniques. (bvsalud.org)
Tension2
Here we developed a method to functionalize DNA tension probes on soft hydrogel surfaces in a controllable and reliable manner, enabling molecular tension fluorescence microscopy for rigidity sensing studies. (bvsalud.org)
Thus, hydrogel-based molecular tension fluorescence microscopy implemented on a standard confocal microscope provides a simple and effective means to explore detailed molecular force information for rigidity-dependent biological processes. (bvsalud.org)
We also demonstrated that ECM rigidity positively regulates the pN force of T cell receptor-ligand bond and T cell receptor mechanical sampling frequency, promoting T cell activation. (bvsalud.org)
Cell1
Talin is a high-molecular-weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts. (wikipedia.org)
I will discuss how the properties under force of integrin-ECM bonds, and of the adaptor protein talin, drive and regulate matrix sensing. (ibecbarcelona.eu)
One of the proteins that control this link is talin, which organizes cytosolic signalling proteins into discrete complexes on ß-integrin tails referred to as focal adhesions (FAs). (bvsalud.org)
Advances in the field of mechanotransduction have demonstrated that focal adhesion complex proteins such as spectrin 6 , talin 7 , and integrin 8 can be deformed, unfolded, and thus activated by forces of physiologic magnitudes. (researchsquare.com)
Recently Kumar et al combined cellular electron cryo-tomography with FRET based tension measurements and find that the regions of high talin tension within focal adhesion have highly aligned and linear underlying filamentous actin structures while regions of low talin tension have less well-aligned actin filaments. (wikipedia.org)
Discovered in 1983 by Keith Burridge and colleagues, talin is a ubiquitous cytosolic protein that is found in high concentrations in focal adhesions. (wikipedia.org)
High affinity1
This structure revealed that the talin binding KN region of KANK1 contains a novel motif where a ß-hairpin stabilizes the α-helical region, explaining both its specific interaction with talin R7 and high affinity. (bvsalud.org)
Actomyosin1
We propose a model whereby actomyosin forces on talin eliminate KANK1 from talin binding in the centre of FAs while retaining it at the adhesion periphery. (bvsalud.org)
Binds1
The adapter protein KANK1 binds to talin in the region of FAs known as the adhesion belt. (bvsalud.org)
Helical1
The hydrophobic residues that define the VBS are themselves 'masked' and are buried in the core of a series of helical bundles that make up the talin rod. (wikipedia.org)
Drives1
Also, talin-1 drives extravasation mechanism through engineered human microvasculature in microfluidic systems. (wikipedia.org)
Domain2
Talin consists of a large C-terminal rod domain that contains bundles of alpha helices and an N-terminal FERM (band 4.1, ezrin, radixin, and moesin) domain with three subdomains: F1, F2, and F3. (wikipedia.org)
Talin also has a middle domain, which has a structure consisting of five alpha helices that fold into a bundle. (wikipedia.org)
Complex1
Here, we adapted a non-covalent crystallographic chaperone to resolve the talin-KANK1 complex. (bvsalud.org)