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  • mitoribosome
  • Here we present the cryo-EM structure analysis of the small (28S) subunit (SSU) of the 55S mitoribosome. (wordpress.com)
  • In addition, docking of previously published coordinates of the large (39S) subunit (LSU) into the cryo-EM map of the 55S mitoribosome reveals that mito-specific MRPs of both the SSU and LSU are involved directly in the formation of six of the 15 intersubunit bridges. (wordpress.com)
  • eIF3
  • Mutating these interactions displays severe growth defects and eliminates association of eIF3i/TIF34 and strikingly also eIF3g/TIF35 with eIF3 and 40S subunits in vivo. (nih.gov)
  • aminoglycoside-induced
  • These findings provide structural and functional insights into aminoglycoside-induced impacts on the eukaryotic ribosome and implicate pleiotropic mechanisms of action beyond decoding. (nih.gov)
  • Our results provide experimental support for aminoglycoside-induced dysfunction of the mitochondrial ribosome. (uzh.ch)
  • PMID
  • It forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits [ PMID: 12446671 , PMID: 15590835 , PMID: 16159874 ]. (ebi.ac.uk)
  • nucleolar
  • In Saccharomyces cerevisiae, Noc4 is involved in nucleolar processing of pre-18S ribosomal RNA and ribosome assembly. (ebi.ac.uk)
  • Aspects of Diamond-Blackfan Anemia, the ribosomopathy that occurs in humans heterozygous for mutations in a number of Rp genes, are thought to reflect chronic p53 signaling, activated by accumulation of a ribosome assembly intermediate and nucleolar stress. (sdbonline.org)
  • Archaea
  • The intersubunit space also appears to be strongly conserved, as might be expected from the degree of functional conservation of the ribosome among kingdoms (Eukarya, Eubacteria, and Archaea). (rupress.org)
  • Much less is known about the process of translation in archaea, but available evidence suggests that they possess a mixture of eubacterial and eukaryotic features. (guwsmedical.info)
  • rRNAs
  • This may be equated with a close contact of the 18S and 28S rRNAs in the translational domain centered on the upper subunit:subunit interface. (rupress.org)
  • Mutations
  • Phenotypic and biochemical analysis of i/TIF34 and b/PRT1 mutations that disrupt subunit interactions. (nih.gov)
  • contrast
  • A new optical device, the volta-phase plate, has been used for smaller molecules by improving contrast by an order of magnitude [ 13 ]. (bioscirep.org)
  • eIF2
  • eIF2 mediates the binding of tRNAmet to the ribosome in a GTP-dependent manner. (acris-antibodies.com)
  • eIF2 is a heterotrimer consisting of an alpha (also called eIF2S1), a beta (eIF2S2), and a gamma (eIF2S3) subunit. (acris-antibodies.com)
  • Since the cellular concentration of eIF2B is much lower than that of eIF2, even a small amount of phosphorylated eIF2 can completely abolish eIF2B activity by sequestration. (acris-antibodies.com)
  • Aminoglycosides
  • However, the sites of interaction of aminoglycosides with the eukaryotic ribosome and their modes of action in eukaryotic translation remain largely unexplored. (nih.gov)
  • Here, we use the combination of X-ray crystallography and single-molecule FRET analysis to reveal the interactions of distinct classes of aminoglycosides with the 80S eukaryotic ribosome. (nih.gov)
  • The distinct impacts of the aminoglycosides examined suggest that their chemical composition and distinct modes of interaction with the ribosome influence PTC read-through efficiency. (nih.gov)
  • Aminoglycosides target the decoding center of the 80S ribosome in a different ways. (nih.gov)