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  • mitoribosome
  • Here we present the cryo-EM structure analysis of the small (28S) subunit (SSU) of the 55S mitoribosome. (wordpress.com)
  • In addition, docking of previously published coordinates of the large (39S) subunit (LSU) into the cryo-EM map of the 55S mitoribosome reveals that mito-specific MRPs of both the SSU and LSU are involved directly in the formation of six of the 15 intersubunit bridges. (wordpress.com)
  • particles
  • However, we found L7 to be a highly specific component of Rlp7-associated complexes, revealing that the two proteins can bind simultaneously to pre-ribosomal particles.Thus, despite their predicted structural similarity, our data show that Rlp7 and L7 clearly bind at different positions on the same pre-60S particles.Our results also suggest that Rlp7 facilitates the formation of the hairpin structure of ITS2 during 60S ribosomal subunit maturation. (nih.gov)
  • Experimental evidence [ PMID: 9371771 ] has revealed that S10 has virtually no groups exposed on the ribosomal surface, and is one of the "split proteins": these are a discrete group that are selectively removed from 30S subunits under low salt conditions and are required for the formation of activated 30S reconstitution intermediate (RI*) particles. (ebi.ac.uk)
  • Preribosomal particles that will mature into 60S and 40S subunits are indicated by the pre-rRNAs contained within them. (nih.gov)
  • The reason behind this usage is because particles of ribosomes were first isolated from cell lysates through the use of a centrifuge. (bioexplorer.net)
  • The names of ribosomes, as well as their sub-particles, were derived from the characteristics of sedimentation during the process of centrifugation. (bioexplorer.net)
  • Nevertheless, the Svedberg (S) units of ribosomes are dependent on the shape and size of the particles and the properties of solutions during sedimentation. (bioexplorer.net)
  • Saccharomyces
  • Shown is the crystal structure of the Saccharomyces cerevisiae 60S subunit viewed from the solvent-accessible side () (PDB accession no. 3U5H and 3U5I). (nih.gov)
  • molecular
  • Translational regulation via L11: molecular switches on the ribosome turned on and off by thiostrepton and micrococcin. (ebi.ac.uk)
  • Since this discovery, the ribosome continued to become one of the pillars where the basis of human knowledge about cellular structures and functions at the biological and molecular level has been constructed. (bioexplorer.net)
  • Two ribosomal subunits are equivalent to 40S and 60S subunits which have molecular masses of 1.5 million g/mol and 3.0 million g/mol respectively? (bioexplorer.net)
  • Ultimately, a " complete " ribosome is called the 80S ribosome , which has a molecular mass of about 4.5 million g/mol. (bioexplorer.net)
  • 2018
  • In fact, mutant organs can be larger than normal, depending on the particular balance of growth between organs (Lee, 2018). (sdbonline.org)
  • functional
  • Although rRNAs build the matrix of the ribosome and carries out catalysing of the peptide-bond formation between amino acids, r-proteins also appear to play important structural and functional roles. (diva-portal.org)
  • Aminoglycosides are chemically diverse, broad-spectrum antibiotics that target functional centers within the bacterial ribosome to impact all four principle stages (initiation, elongation, termination, and recycling) of the translation mechanism. (nih.gov)
  • These findings provide structural and functional insights into aminoglycoside-induced impacts on the eukaryotic ribosome and implicate pleiotropic mechanisms of action beyond decoding. (nih.gov)
  • The intersubunit space also appears to be strongly conserved, as might be expected from the degree of functional conservation of the ribosome among kingdoms (Eukarya, Eubacteria, and Archaea). (rupress.org)
  • genes
  • Aspects of Diamond-Blackfan Anemia, the ribosomopathy that occurs in humans heterozygous for mutations in a number of Rp genes, are thought to reflect chronic p53 signaling, activated by accumulation of a ribosome assembly intermediate and nucleolar stress. (sdbonline.org)
  • complexity
  • We also explore the increasing complexity of ribosome biology and how this informs our understanding of developmental biology and human disease. (bloodjournal.org)